Objective To identify genes that may be related to embryo implantation Materials&Methods The PCR subtraction technique was applied at implantation and inter-plantation sites on day 4.5 of pregnancy in mice.Two nov...Objective To identify genes that may be related to embryo implantation Materials&Methods The PCR subtraction technique was applied at implantation and inter-plantation sites on day 4.5 of pregnancy in mice.Two novel Expressed Sequence Tags(ESTs),EST8 and EST81 were identified;their expression in tissues was analyzed by Northern blotting,and their full-length cDNAs were synthesized by PCR.Results We found that these two novel ESTs(EST8and EST81)were noticeably expressed in implantation site in the mouse on day 4.5 of pregnancy.EST8 was expressed at high level in livers and implantation sites of the mice,while at low level in ovaries and inter-plantation sites.EST81 was predominantly expressed in implantation site and ovary,and at low level in all other tissues.Their complete cDNAs,1665bp and 1264 bp respectively,were synthesized by using PCR.Conclusion The two full-length cDNAs were responsible for embryo implantation,and their functions need to be further studied.展开更多
基金Supported by the National Key Basic Research Special Funds(No.G1999055903)
文摘Objective To identify genes that may be related to embryo implantation Materials&Methods The PCR subtraction technique was applied at implantation and inter-plantation sites on day 4.5 of pregnancy in mice.Two novel Expressed Sequence Tags(ESTs),EST8 and EST81 were identified;their expression in tissues was analyzed by Northern blotting,and their full-length cDNAs were synthesized by PCR.Results We found that these two novel ESTs(EST8and EST81)were noticeably expressed in implantation site in the mouse on day 4.5 of pregnancy.EST8 was expressed at high level in livers and implantation sites of the mice,while at low level in ovaries and inter-plantation sites.EST81 was predominantly expressed in implantation site and ovary,and at low level in all other tissues.Their complete cDNAs,1665bp and 1264 bp respectively,were synthesized by using PCR.Conclusion The two full-length cDNAs were responsible for embryo implantation,and their functions need to be further studied.
