The purpose of this research is to create a simulated environment for teaching algorithms,big data processing,and machine learning.The environment is similar to Google Maps,with the capacity of finding the fastest pat...The purpose of this research is to create a simulated environment for teaching algorithms,big data processing,and machine learning.The environment is similar to Google Maps,with the capacity of finding the fastest path between two points in dynamic traffic situations.However,the system is significantly simplified for educational purposes.Students can choose different traffic patterns and program a car to navigate through the traffic dynamically based on the changing traffic.The environments used in the project are Visual IoT/Robotics Programming Language Environment(VIPLE)and a traffic simulator developed in the Unity game engine.This paper focuses on creating realistic traffic data for the traffic simulator and implementing dynamic routing algorithms in VIPLE.The traffic data are generated from the recorded real traffic data published on the Arizona Maricopa County website.Based on the generated traffic data,VIPLE programs are developed to implement the traffic simulation with support for dynamic changing data.展开更多
Staphylococcus aureus produces staphyloxanthin,a C30 carotenoid with golden color,as an antioxidant to promote bacterial resistance to reactive oxygen species.The biosynthesis pathway of staphyloxanthin involves a ser...Staphylococcus aureus produces staphyloxanthin,a C30 carotenoid with golden color,as an antioxidant to promote bacterial resistance to reactive oxygen species.The biosynthesis pathway of staphyloxanthin involves a series of catalytic enzymes.Aldehyde dehydrogenase(AldH)is a dehydrogenase recently identified to convert 4,4’-diaponeurosporenaldehyde into 4,4’-diaponeurosporenoic acid during staphyloxanthin biosynthesis.Here,we present the crystallographic structures of apo-and holo-forms of S.aureus AldH.The dimeric enzyme contains a unique C-terminal helix,which resembles a“gatekeeper”helix found in human membrane-bound fatty aldehyde dehydrogenase(FALDH).Particularly,the helix adopts“open”and“closed”conformations in apo-and holo-AldH,respectively,to control the access of the substrate tunnel.Mutagenesis in combination with in vitro and in vivo activity assays identifies several residues essential for S.aureus AldH substrate recognition and enzyme catalytic turnover.Our results provide insights into substrate recognition of S.aureus AldH toward polyunsaturated long-chain aldehydes at atomic resolution.展开更多
文摘The purpose of this research is to create a simulated environment for teaching algorithms,big data processing,and machine learning.The environment is similar to Google Maps,with the capacity of finding the fastest path between two points in dynamic traffic situations.However,the system is significantly simplified for educational purposes.Students can choose different traffic patterns and program a car to navigate through the traffic dynamically based on the changing traffic.The environments used in the project are Visual IoT/Robotics Programming Language Environment(VIPLE)and a traffic simulator developed in the Unity game engine.This paper focuses on creating realistic traffic data for the traffic simulator and implementing dynamic routing algorithms in VIPLE.The traffic data are generated from the recorded real traffic data published on the Arizona Maricopa County website.Based on the generated traffic data,VIPLE programs are developed to implement the traffic simulation with support for dynamic changing data.
基金supported by the National Natural Science Foundation of China(21671203 and 21877131)RGC of Hong Kong(17305415 and 17333616)+1 种基金the Ministry of Education of China(IRT-17R111)the Fundamental Research Funds for the Central Universities.
文摘Staphylococcus aureus produces staphyloxanthin,a C30 carotenoid with golden color,as an antioxidant to promote bacterial resistance to reactive oxygen species.The biosynthesis pathway of staphyloxanthin involves a series of catalytic enzymes.Aldehyde dehydrogenase(AldH)is a dehydrogenase recently identified to convert 4,4’-diaponeurosporenaldehyde into 4,4’-diaponeurosporenoic acid during staphyloxanthin biosynthesis.Here,we present the crystallographic structures of apo-and holo-forms of S.aureus AldH.The dimeric enzyme contains a unique C-terminal helix,which resembles a“gatekeeper”helix found in human membrane-bound fatty aldehyde dehydrogenase(FALDH).Particularly,the helix adopts“open”and“closed”conformations in apo-and holo-AldH,respectively,to control the access of the substrate tunnel.Mutagenesis in combination with in vitro and in vivo activity assays identifies several residues essential for S.aureus AldH substrate recognition and enzyme catalytic turnover.Our results provide insights into substrate recognition of S.aureus AldH toward polyunsaturated long-chain aldehydes at atomic resolution.
