Lanostane Triterpenoids from Fruiting Bodies of Ganoderma leucocontextum

Six new lanostane-type triterpenoids,namely leucocontextins S-X(1-6),together with twelve known compounds,were isolated from the fruiting bodies of Ganoderma leucocontextum.Their structures were established by MS and NMR data.

Steroids and Sesquiterpenes From Cultures of the Fungus Phellinus igniarius

Two new steroids,3a,17a,19,20-tetrahydroxy-4a-methylpregn-8-ene(1)and 3a,12a,17a,20-tetrahydroxy-4amethylpregn-8-ene(2)and three new sesquiterpenoids,12-hydroxy-a-cadinol(3),3a,12-dihydroxy-d-cadinol(4),and 3a,6a-dihydroxyspiroax-4-ene(5),have been isolated from cultures of the fungus Phellinus igniarius.Their structures were characterized based on extensive spectroscopic data.In preliminary in vitro assays,compounds 3 and 4 exhibited the vascular-activities against phenylephrine-induced vasoconstriction with the relaxing rates of 11.0%and 7.0%at 3910-4 M,respectively.

Irpexoates A-D,Four Triterpenoids with Malonyl Modifications from the Fruiting Bodies of the Medicinal Fungus Irpex lacteus

Four eburicane-type triterpenoids with malonyl modifications,namely irpexoates A-D(1-4),were isolated from the fruiting bodies of the medicinal fungus Irpex lacteus.The structures of the new compounds were established by extensive spectroscopic methods,including 1D and 2D NMR,HRESIMS spectroscopic analysis.Irpexoate B(2)displayed weak cytotoxicity against four human cancer cell lines(A-549,SMMC-7721,MCF-7,SW480)with IC50 values varying from 22.9 to 34.0 pM,and irpexoate D(4)showed weak cytotoxicity against the human cancer cell line SW480 with an IC50 value of 35.2μM.

Four New Sesquiterpenoids from Cultures of the Fungus Phellinidium sulphurascens

Four new sesquiterpenoids,namely 12-hydroxy-3-oxodrimenol(1),11-hydroxyacetoxydrim-7-en-3b-ol(2),2,6-dimethyl-7,10-epoxy-10-hydroxymethyldodeca-2,11-dien-6-ol(3),and 7,10-epoxy-2,6,10-trimethyldodeca-2,11-diene-4,6-diol(4),along with fourteen known compounds,were isolated from the cultures of Phellinidium sulphurascens.The structures of compounds 1–4 were established on the basis of extensive spectroscopic analysis.All of them were evaluated for their cytotoxic activities.

Six New Vibralactone Derivatives from Cultures of the Fungus Boreostereum vibrans

Phytochemical reinvestigation on the cultural broth of Boreostereum vibrans led to the isolation of six new vibralactone derivatives,vibralactone N(1),vibralactone O(2),vibralactone P(3),10-lactyl vibralactone G(4),(3S*,4R*)-6-acetoxymethyl-2,2-dimethyl-3,4-dihydro-2H-chromene-3,4-diol(5),vibralactone Q(6).Their structures were elucidated by extensive spectroscopic methods.

Vibralactone Biogenesis-Associated Analogues from Submerged Cultures of the Fungus Boreostereum vibrans

A scale-up fermentation of the fungus Boreostereum vibrans facilitated the isolation of six new vibralactone biogenesis-associated analogues,namely vibralactamide A(1),vibralactone T(2),13-O-lactyl vibralactone(3),10-O-acetyl vibralactone G(4),(11R,12R)-and(11S,12R)-vibradiol(5,6).Their structures were established via extensive spectroscopic analyses,specific optical rotation comparison,and Snatzke’s method.The biosynthetic pathway for vibralactamide A was postulated.The absolute configuration of vibralactone B was revised by single crystal X-ray diffraction analysis.This work puts the divergent vibralactone biosynthesis pathway one step further and expands the structural diversity of vibralactone-associated compounds.

Seco-tremulane Sesquiterpenoids from the Cultures of the Medicinal Fungus Irpex lacteus HFG1102

Six previously undescribed 5,6-seco-tremulane analogues,together with two known ones,were isolated from the culture broth of the medicinal fungus Irpex lacteus HFG1102.The structures of the new compounds were elucidated via extensive spectroscopic methods,including NMR and HRMS spectroscopic analyses.

Fissisternoids A and B,two 2’,5’-quinodihydrochalcone-based meroterpenoid enantiomers with unusual carbon skeletons from Fissistigma bracteolatum

Two pairs of meroterpenoid enantiomers,(±)-fissisternoids A(1)and B(2),along with their putative biogenetic precursor 3 were isolated from the branches and leaves of Fissistigma bracteolatum.Compound 1 represents an unprecedented meroterpenoid featuring a unique tricyclo[3,3,1,01’,5’]decane central framework,and compound 2 possesses a rare 6/6/5/4 tetracyclic carbon skeleton,both of which were derived from quinodihydrochalcone and monoterpenoid via a key[4+2]Diels–Alder cyclization and Prins reaction.Their structures and absolute configurations were established using spectroscopic data,X-ray diffraction,and electronic circular dichroism calculations.Compounds 1 and 2 exhibit anti-inflammatory activity(IC50:13.2μM,1;9.8μM,2)via suppression of inflammatory cytokines IL-1β,IL-6,and iNOS.