Construction of mice ex vivo spinal cord slice model of neuromyelitis optica induced by NMO-immunoglobulin G

Objective:Using neuromyelitis optica immunoglobulin G(NMO-IgG)to induced ex vivo mice spinal cord slice model.Methods:Vibratome-cut transverse spinal cord slices from 7-day-old C57BL/6Jmouse pups were cultured on transwell porous supports for 7days,then randomly divided into the control group and NMO model group.Slices of the control group were further cultured with human serum complement,while slices from NMO model group were exposed to complement and NMO-IgG.After 24-hour incubation,slices of both groups were measured for aquaporin-4(AQP4),glial fibrillary acidic protein(GFAP),myelin basic protein(MBP)and neurofilament light chain(NFL)by immunofluorescence.Results:Slices exposed to NMO-IgG showed astrocyte swelling,and a significant loss of AQP4and GFAP staining.Ratios of the loss of AQP4and GFAP staining were 77.74%±6.75%and 75.62%±5.76%respectively in the model group,and NMO-like injury score was 3.11±0.60.But there were no obvious losses of AQP4and GFAP staining in the control group,and NMO-like lesion score was 0.00.There were significant differences between the two groups with regards to the above indexes(P<0.01).Ratios of the loss of MBP and NFL staining in the model group were 37.60%±4.88%and46.29%±4.98%respectively,while the corresponding figures in the control group were 9.10%±1.63%and 5.80%±0.81%,and the differences between the two groups were statistically significant(P<0.01).Conclusion:These results suggested that NMO-IgG-induced ex vivo spinal cord slice model possesses typical features of NMO,and this model might be useful for relevant fundamental studies.

细菌降解蒽醌染料研究进展（英文）

本文综述了近年来细菌降解蒽醌染料的研究进展及机理,以期为蒽醌染料废水的实际处理提供理论依据。目前主要利用物理、化学及生物法处理工业印染废水中的各种染料。与前两者相比,生物法具有经济且环保的特点。本文以蒽醌染料的分类及结构为基础,总结近年来已报道的蒽醌染料高效降解细菌的多样性;初步探讨细菌吸附、降解蒽醌染料的机理与主要影响因素;根据目前的研究进展及存在问题,提出细菌降解蒽醌染料的研究方向。