Puccinia striiformis f. sp. tritici （Pst） is the obligate biotrophic fungus responsible for stripe rust wheat. In this study, we developed and characterized 20 polymorphic microsatelUte markers from the genomic sequ...Puccinia striiformis f. sp. tritici （Pst） is the obligate biotrophic fungus responsible for stripe rust wheat. In this study, we developed and characterized 20 polymorphic microsatelUte markers from the genomic sequence of an isolate of Chinese Pst race C^32. Polymorphism at each simple sequence repeat （SSR） locus was determined using 32 Pst isolates from 7 countries. The number of alleles varied from 2 to 7 across isolates, and the observed and expected heterozygosities ranged from 0.33 to 0.97 （mean 0.62） and 0.23 to 0.73 （mean 0.51 ）, respectively. As expected the genomic SSR markers were more polymorphic than the expressed sequence tag （EST）-SSR markers developed previously. These markers will be more useful for population genetics and molecular genetics studies in Pst.展开更多
We are pleased to invite you to join us in celebrating the birth of a new life sciences academic journal,Stress Biology.Stress Biology was launched this year as an open access journal published by Springer Nature in p...We are pleased to invite you to join us in celebrating the birth of a new life sciences academic journal,Stress Biology.Stress Biology was launched this year as an open access journal published by Springer Nature in partnership with Northwest A&F University of China.We plan to have the journal indexed by SCIE in 2024 and aim to obtain its first impact factor of no less than 5.0 in 2024.展开更多
基金supported by the National Basic Research Program(973)of China(No.2013CB127700)the Special Fund for Agro-Scientific Research in the Public Interest(No.201303023)+1 种基金the National Natural Science Foundation of China(No.31371882)the 111 Project from the Ministry of Education of China(No.B07049)
文摘Puccinia striiformis f. sp. tritici （Pst） is the obligate biotrophic fungus responsible for stripe rust wheat. In this study, we developed and characterized 20 polymorphic microsatelUte markers from the genomic sequence of an isolate of Chinese Pst race C^32. Polymorphism at each simple sequence repeat （SSR） locus was determined using 32 Pst isolates from 7 countries. The number of alleles varied from 2 to 7 across isolates, and the observed and expected heterozygosities ranged from 0.33 to 0.97 （mean 0.62） and 0.23 to 0.73 （mean 0.51 ）, respectively. As expected the genomic SSR markers were more polymorphic than the expressed sequence tag （EST）-SSR markers developed previously. These markers will be more useful for population genetics and molecular genetics studies in Pst.
文摘We are pleased to invite you to join us in celebrating the birth of a new life sciences academic journal,Stress Biology.Stress Biology was launched this year as an open access journal published by Springer Nature in partnership with Northwest A&F University of China.We plan to have the journal indexed by SCIE in 2024 and aim to obtain its first impact factor of no less than 5.0 in 2024.
