<strong>Purpose:</strong> The aim of the present study was to determine the degree of using floss and the demographic characteristics of medical faculties of Inner Mongolia Medical University. <strong&g...<strong>Purpose:</strong> The aim of the present study was to determine the degree of using floss and the demographic characteristics of medical faculties of Inner Mongolia Medical University. <strong>Materials and Methods:</strong> Participants completed the questionnaire in the classroom. These participants came from the medical faculties of Chinese Medicine and Western Medicine. These participants were interviewed to determine the demographic characteristics. Non-flossors responded according to their reasons for not flossing. <strong>Results:</strong> A total of 3481 effective data were available for analysis. The floss prevalence was 17.21%, which was lower by 5.72% for students living in rural areas, when compared to students living in urban areas. The largest proportion of non-flossing participants in rural areas answered, “I do not know what a floss is”, while participants in urban answered, “I do not want to use it”. The largest proportion of answered flossing frequency was less than once a month. <strong>Conclusion:</strong> The present study indicates that the prevalence of using floss in medical faculties in Inner Mongolia Medical University was higher, when compared to that in Sichuan province, China, but lower than that in other countries. Although the students use floss, more than one-third of students use floss less than once a month, which is close to “never”. The prevalence in rural areas is lower than those in urban areas. Male not only comprises the total number of prevalence, but also has a frequency greater than that in females. The emphasis on using floss should be improved in medical students.展开更多
Aggregation-induced emission(AIE)materials offer promising perspectives in disease diagnosis and therapeutics given their unique optical and photochemical properties.A key step toward translational applications for AI...Aggregation-induced emission(AIE)materials offer promising perspectives in disease diagnosis and therapeutics given their unique optical and photochemical properties.A key step toward translational applications for AIE materials is to systematically and vigorously evaluate their biosafety and biocompatibility.While previous studies focus on cellular viability and toxicity,the impact of AIE materials on detailed stress responses manifesting cellular fitness has been less explored.Herein,this work provides the first piece of evidence to support amphiphilic functionalization of AIE nanoparticles minimizes the deterioration on proteome stability and cellular protein homeostasis(proteostasis).To this end,four scaffolds of AIE molecules were prepared,further functionalized into eight nanoparticles with two amphiphilic shells respectively,and characterized for their physicochemical properties.Thermal shift assay quantitatively demonstrates that AIE materials after amphiphilic functionalization into nanoparticles enhance proteome thermodynamic stability and ameliorate proteome aggregation propensity in cellular lysate,echoed by cell viability and fractionation experiments.Intriguingly,poor polydispersity index(PDI)of functionalized nanoparticles exaggerates their retention and aggregation in the cell.Comparative proteomic analysis uncovers that amphiphilic functionalization of AIE materials can minimize the deterioration of cellular protein homeostasis network.Finally,vigorous interrogation of functionalized AIE nanoparticles in mice model reveals the complexity of factors affecting the biocompatibility profiles in vivo,including materials’size,PDI,and treatment frequencies.Overall,amphiphilic functionalization of AIE materials into nanoparticles is necessary to maintain proteome stability and balance cellular protein homeostasis.展开更多
文摘<strong>Purpose:</strong> The aim of the present study was to determine the degree of using floss and the demographic characteristics of medical faculties of Inner Mongolia Medical University. <strong>Materials and Methods:</strong> Participants completed the questionnaire in the classroom. These participants came from the medical faculties of Chinese Medicine and Western Medicine. These participants were interviewed to determine the demographic characteristics. Non-flossors responded according to their reasons for not flossing. <strong>Results:</strong> A total of 3481 effective data were available for analysis. The floss prevalence was 17.21%, which was lower by 5.72% for students living in rural areas, when compared to students living in urban areas. The largest proportion of non-flossing participants in rural areas answered, “I do not know what a floss is”, while participants in urban answered, “I do not want to use it”. The largest proportion of answered flossing frequency was less than once a month. <strong>Conclusion:</strong> The present study indicates that the prevalence of using floss in medical faculties in Inner Mongolia Medical University was higher, when compared to that in Sichuan province, China, but lower than that in other countries. Although the students use floss, more than one-third of students use floss less than once a month, which is close to “never”. The prevalence in rural areas is lower than those in urban areas. Male not only comprises the total number of prevalence, but also has a frequency greater than that in females. The emphasis on using floss should be improved in medical students.
基金National Natural Science Foundation of China,Grant/Award Numbers:22107100,21907091,21725506China Postdoctoral Science Foundation,Grant/Award Number:2019M661138+2 种基金LiaoNing Revitalization Talents Program from the Liaoning province of China,Grant/Award Number:XLYC1907048Dalian Innovation Fund,Grant/Award Number:2020JJ26GX027Youth Innovation Promotion Association,CAS,Grant/Award Number:2020184。
文摘Aggregation-induced emission(AIE)materials offer promising perspectives in disease diagnosis and therapeutics given their unique optical and photochemical properties.A key step toward translational applications for AIE materials is to systematically and vigorously evaluate their biosafety and biocompatibility.While previous studies focus on cellular viability and toxicity,the impact of AIE materials on detailed stress responses manifesting cellular fitness has been less explored.Herein,this work provides the first piece of evidence to support amphiphilic functionalization of AIE nanoparticles minimizes the deterioration on proteome stability and cellular protein homeostasis(proteostasis).To this end,four scaffolds of AIE molecules were prepared,further functionalized into eight nanoparticles with two amphiphilic shells respectively,and characterized for their physicochemical properties.Thermal shift assay quantitatively demonstrates that AIE materials after amphiphilic functionalization into nanoparticles enhance proteome thermodynamic stability and ameliorate proteome aggregation propensity in cellular lysate,echoed by cell viability and fractionation experiments.Intriguingly,poor polydispersity index(PDI)of functionalized nanoparticles exaggerates their retention and aggregation in the cell.Comparative proteomic analysis uncovers that amphiphilic functionalization of AIE materials can minimize the deterioration of cellular protein homeostasis network.Finally,vigorous interrogation of functionalized AIE nanoparticles in mice model reveals the complexity of factors affecting the biocompatibility profiles in vivo,including materials’size,PDI,and treatment frequencies.Overall,amphiphilic functionalization of AIE materials into nanoparticles is necessary to maintain proteome stability and balance cellular protein homeostasis.