Understanding of genetic diversity among sengon(paraseranthes falcataria)is very important for selection and improvement of its breeding.This study determined the genetic diversity among the previous preferred 36 clon...Understanding of genetic diversity among sengon(paraseranthes falcataria)is very important for selection and improvement of its breeding.This study determined the genetic diversity among the previous preferred 36 clones which selected from 6 districts.Of 8 Paraserianthes falcataria SSR markers and 76 SSR markers selected from Acacia species,only 13 markers showed polymorphic pattern and amplified a total of 61 alleles.The polymorphism information content(PIC)value at a locus ranged from 0.099 to 0.799.The average number of alleles per locus ranged from 2 to 9 with an average of 4.92.Six SSR markers were highly informative and had PIC values above 0.60.The results demonstrated that the SSR DNA-markers developed from Acacia species could be successfully used to differentiate genotypes of P.falcataria.Cluster analysis of SSR data using UPGMA separated 36 clones into 2 divergent clusters at similarity coefficient of 0.56.The diverse clones identified in the study would be useful for developing intraspecific hybrids to exploit hybrid vigour and for commercial cultivation to broaden genetic base.Meanwhile,the DNA fingerprints obtained for each clone can be used for identification of breeding population and accelerate research of P.falcataria breeding.展开更多
文摘Understanding of genetic diversity among sengon(paraseranthes falcataria)is very important for selection and improvement of its breeding.This study determined the genetic diversity among the previous preferred 36 clones which selected from 6 districts.Of 8 Paraserianthes falcataria SSR markers and 76 SSR markers selected from Acacia species,only 13 markers showed polymorphic pattern and amplified a total of 61 alleles.The polymorphism information content(PIC)value at a locus ranged from 0.099 to 0.799.The average number of alleles per locus ranged from 2 to 9 with an average of 4.92.Six SSR markers were highly informative and had PIC values above 0.60.The results demonstrated that the SSR DNA-markers developed from Acacia species could be successfully used to differentiate genotypes of P.falcataria.Cluster analysis of SSR data using UPGMA separated 36 clones into 2 divergent clusters at similarity coefficient of 0.56.The diverse clones identified in the study would be useful for developing intraspecific hybrids to exploit hybrid vigour and for commercial cultivation to broaden genetic base.Meanwhile,the DNA fingerprints obtained for each clone can be used for identification of breeding population and accelerate research of P.falcataria breeding.
