Host-plant volatiles play an important role as cues for herbivores in search of resources, mates and oviposition sites in complex environments. Plant volatile-based attractants can be developed for pest monitoring and...Host-plant volatiles play an important role as cues for herbivores in search of resources, mates and oviposition sites in complex environments. Plant volatile-based attractants can be developed for pest monitoring and control. Previously, we indicated that mated female adults of Callosobruchus chinensis showed choice preference behavior toward 2-hexenal and benzaldehyde. Our objective here was to investigate the synergistic effect of host-derived attractive volatiles in attracting C. chinensis under laboratory and field conditions in Shanxi Province, China. We hypothesized that the ratio and concentration of volatiles derived from Vigna radiata play critical roles for C. chinensis in locating this host. Therefore, we collected and identified the volatiles of mungbean by using headspace collection and GC-MS. The effectiveness of different ratios and concentrations of two compounds (2-hexenal and benzaldehyde) that elicit C. chinensis searching behavior were examined in Y-tube olfactometer assays. The combination of 300 μg μL^–1 2-hexenal and 180 μg μL^–1 benzaldehyde loadings exhibited a synergistic effect on attracting C. chinensis (82.35%). Compared to control traps, the adults were significantly attracted to traps baited with blends, and more attraction to females than males was found for blend traps in the field experiments. Our results suggest that blends of this specific concentration and ratio of benzaldehyde and 2-hexenal can be used in traps as attractants for C. chinensis monitoring and control in the field.展开更多
The nutrient status and dynamics of three clover （Trifolium spp.） varieties at different growth stages were investigated with the crude protein content as an indicator of nutrient equilibrium. The crude protein, neu...The nutrient status and dynamics of three clover （Trifolium spp.） varieties at different growth stages were investigated with the crude protein content as an indicator of nutrient equilibrium. The crude protein, neutral detergent fiber （NDF）, phosphorus （P）, and calcium （Ca） content followed a decreasing trend at the initial growing stage and increased later. NDF and Ca content peaked in May, while crude protein and P content reached the highest in May and the lowest in July. The crude ash content followed an uncertain pattern as plants growing. The ash content of both wild-type red clover （T. pratensis L.） and ＇Sativum＇ decreased at the beginning and then increased. However, the ash content of ＇Revendef white clover （T. repens L.） decreased all through the experimental period. The general trend was observed for all three clover varieties as the ash content was the highest at vegetative growing stage （May）, and the acidic detergent fiber （ADF） content increased with time. The nutrient equilibrium value was ranked as ＇Revendei＇〉wild-type red clover〉＇Sativum＇.展开更多
基金This research was funded by the Shanxi Provincial Key Research and Development Project,China(201803D221004-8)the earmarked fund for China Agriculture Research System(CARS-08-G10)+1 种基金the Research and Demonstration of Key Green Technology for High Quality of Coarse Cereals in Northwest Shanxi(201703D211002-8)the Shanxi Province Science Foundation for Youths(201801D221305).
文摘Host-plant volatiles play an important role as cues for herbivores in search of resources, mates and oviposition sites in complex environments. Plant volatile-based attractants can be developed for pest monitoring and control. Previously, we indicated that mated female adults of Callosobruchus chinensis showed choice preference behavior toward 2-hexenal and benzaldehyde. Our objective here was to investigate the synergistic effect of host-derived attractive volatiles in attracting C. chinensis under laboratory and field conditions in Shanxi Province, China. We hypothesized that the ratio and concentration of volatiles derived from Vigna radiata play critical roles for C. chinensis in locating this host. Therefore, we collected and identified the volatiles of mungbean by using headspace collection and GC-MS. The effectiveness of different ratios and concentrations of two compounds (2-hexenal and benzaldehyde) that elicit C. chinensis searching behavior were examined in Y-tube olfactometer assays. The combination of 300 μg μL^–1 2-hexenal and 180 μg μL^–1 benzaldehyde loadings exhibited a synergistic effect on attracting C. chinensis (82.35%). Compared to control traps, the adults were significantly attracted to traps baited with blends, and more attraction to females than males was found for blend traps in the field experiments. Our results suggest that blends of this specific concentration and ratio of benzaldehyde and 2-hexenal can be used in traps as attractants for C. chinensis monitoring and control in the field.
基金Supported by the Science and Technology Foundation(2008BADB3B09-03)
文摘The nutrient status and dynamics of three clover （Trifolium spp.） varieties at different growth stages were investigated with the crude protein content as an indicator of nutrient equilibrium. The crude protein, neutral detergent fiber （NDF）, phosphorus （P）, and calcium （Ca） content followed a decreasing trend at the initial growing stage and increased later. NDF and Ca content peaked in May, while crude protein and P content reached the highest in May and the lowest in July. The crude ash content followed an uncertain pattern as plants growing. The ash content of both wild-type red clover （T. pratensis L.） and ＇Sativum＇ decreased at the beginning and then increased. However, the ash content of ＇Revendef white clover （T. repens L.） decreased all through the experimental period. The general trend was observed for all three clover varieties as the ash content was the highest at vegetative growing stage （May）, and the acidic detergent fiber （ADF） content increased with time. The nutrient equilibrium value was ranked as ＇Revendei＇〉wild-type red clover〉＇Sativum＇.
