Long-term survival and risk factors in esophageal squamous cell carcinoma:A Kaplan-Meier and cox regression study

BACKGROUND The global incidence of esophageal cancer(EC)remains high.Despite advan-cements in medical technology and deeper research into the causes and treatment methods of EC,the effectiveness of treatment for EC is still unsatisfactory.Therefore,it is crucial to address the urgent problem of improving the long-term survival rate of EC patients and providing personalized treatment.AIM To analyze the survival prognosis and influencing factors of esophageal squamous cell carcinoma(ESCC).METHODS A retrospective analysis was conducted on the clinical data of 115 patients with pT3N0M0 ESCC who underwent radical surgery alone from January 1,2013,to December 31,2019.The Kaplan–Meier method was used to evaluate the 1-year,3-year,and 5-year survival rates and median survival time of the patients.The Cox proportional hazards regression model was used to assess the hazard ratios(HRs)and 95%confidence intervals(95%CIs)of risk factors.RESULTS The 1-year,3-year,and 5-year overall survival(OS)rates for the 115 EC patients analyzed were 85.22%,50.43%,and 37.48%,respectively.The median OS was 37.00(95%CI:24.93-49.07)months,and the median disease-free survival was 21.00(95%CI:14.71-27.29)months.Both univariate and multivariate Cox regression analyses revealed that high body mass index(BMI;HR=1.137,95%CI:1.054-1.226),positive perineural invasion(PNI;HR=13.381,95%CI:4.899-36.547),and smoking(HR=2.415,95%CI:1.388-4.203)were independent risk factors for a poor prognosis.In contrast,compared to the upper thoracic location of the tumor,middle thoracic(HR=0.441,95%CI:0.240-0.810)and lower thoracic(HR=0.328,95%CI:0.144-0.750)locations were protective factors.CONCLUSION BMI,tumor location,PNI,and smoking are associated with the prognosis of ESCC patients.This study highlights the prognostic risk factors for T3N0M0 ESCC patients and offers personalized insights for clinical treatment.

Influence of paeonol combined with γ ray on glioma cell apoptosis and apoptosis gene expressiona

Objective: To study the influence of paeonol combined with γ ray on glioma cell apoptosis and apoptosis gene expression. Methods: Glioma C6 cells were cultured and divided into control group (treated with serum-free DMEM), ray group (exposed to 4 Gy γ ray), Pae group (treated with serum-free DMEM contains 4 μg/mL paeonol) and γ+Pae group (treated with serum-free DMEM contains 4 μg/mL paeonol and exposed to 4 Gy γ ray). The cell proliferation activity value as well as the expression of pro-apoptosis genes and anti-apoptosis genes was measured after the intervention. Results: After 6 h, 12 h, 18 h and 24 h of intervention, cell proliferation activity value of ray group, Pae group and γ+Pae group were greatly lower than that of control group, and cell proliferation activity value of γ+Pae group was obviously lower than that of ray group and Pae group. After 24 h of intervention, p21cip1, TRAIL, LRIG1, NPRL2 and SEPT7 mRNA expression in ray group, Pae group and γ+Pae group were notably higher than those in control group whereas PIAS3, CEACAM1, EZH2, MDM2, c-myc and Survivin mRNA expression were greatly lower than those in control group;p21cip1, TRAIL, LRIG1, NPRL2 and SEPT7 mRNA expression in γ+Pae group were remarkably higher than those in ray group and Pae group whereas PIAS3, CEACAM1, EZH2, MDM2, c-myc and Survivin mRNA expression were greatly lower than those in ray group and Pae group. Conclusion: Paeonol combined with γ ray can be more effective than γ ray alone in inducing the apoptosis of glioma cells, increasing the expression of pro-apoptosis genes and decreasing the expression of anti-apoptosis genes.