Polyploids contribute substantially to plant evolution and biodiversity;however,the mechanisms by which they succeed are still unclear.According to the polyploid adaptation hypothesis,successful polyploids spread by r...Polyploids contribute substantially to plant evolution and biodiversity;however,the mechanisms by which they succeed are still unclear.According to the polyploid adaptation hypothesis,successful polyploids spread by repeated adaptive responses to new environments.Here,we tested this hypothesis using two tetraploid yellowcresses(Rorippa),the endemic Rorippa elata and the widespread Rorippa palustris,in the temperate biodiversity hotspot of the Hengduan Mountains.Speciation modes were resolved by phylogenetic modeling using 12 low-copy nuclear loci.Phylogeographical patterns were then examined using haplotypes phased from four plastid and ITS markers,coupled with historical niche reconstruction by ecological niche modeling.We inferred the time of hybrid origins for both species as the mid-Pleistocene,with shared glacial refugia within the southern Hengduan Mountains.Phylogeographic and ecological niche reconstruction indicated recurrent northward colonization by both species after speciation,possibly tracking denuded habitats created by glacial retreat during interglacial periods.Common garden experiment involving perennial R.elata conducted over two years revealed significant changes in fitness-related traits across source latitudes or altitudes,including latitudinal increases in survival rate and compactness of plant architecture,suggesting gradual adaptation during range expansion.These findings support the polyploid adaptation hypothesis and suggest that the spread of polyploids was aided by adaptive responses to environmental changes during the Pleistocene.Our results thus provide insight into the evolutionary success of polyploids in high-altitude environments.展开更多
基金supported by the National Natural Science Foundation of China(31800177,32170224,and U1802242)the Strategic Priority Research Program of Chinese Academy of Sciences(XDB31000000)+2 种基金T-S.H.is also supported by the Youth Innovation Promotion Association CAS(2020391)China Scholarship Council(201804910061)CAS Light of West China Program.We acknowledge Ya-Long Guo at the Institute of Botany CAS for providing Capsella rubella seeds and revising the manuscript.
文摘Polyploids contribute substantially to plant evolution and biodiversity;however,the mechanisms by which they succeed are still unclear.According to the polyploid adaptation hypothesis,successful polyploids spread by repeated adaptive responses to new environments.Here,we tested this hypothesis using two tetraploid yellowcresses(Rorippa),the endemic Rorippa elata and the widespread Rorippa palustris,in the temperate biodiversity hotspot of the Hengduan Mountains.Speciation modes were resolved by phylogenetic modeling using 12 low-copy nuclear loci.Phylogeographical patterns were then examined using haplotypes phased from four plastid and ITS markers,coupled with historical niche reconstruction by ecological niche modeling.We inferred the time of hybrid origins for both species as the mid-Pleistocene,with shared glacial refugia within the southern Hengduan Mountains.Phylogeographic and ecological niche reconstruction indicated recurrent northward colonization by both species after speciation,possibly tracking denuded habitats created by glacial retreat during interglacial periods.Common garden experiment involving perennial R.elata conducted over two years revealed significant changes in fitness-related traits across source latitudes or altitudes,including latitudinal increases in survival rate and compactness of plant architecture,suggesting gradual adaptation during range expansion.These findings support the polyploid adaptation hypothesis and suggest that the spread of polyploids was aided by adaptive responses to environmental changes during the Pleistocene.Our results thus provide insight into the evolutionary success of polyploids in high-altitude environments.
