Cytoplasmic male sterility(CMS)is an important tool for producing F1 hybrids,which can exhibit heterosis.The companion system,restorer-of-fertility(Rf),is poorly understood at the molecular level and would be valuable...Cytoplasmic male sterility(CMS)is an important tool for producing F1 hybrids,which can exhibit heterosis.The companion system,restorer-of-fertility(Rf),is poorly understood at the molecular level and would be valuable in producing restorer lines for hybrid seed production.The identity of the Rf gene in Capsicum(pepper)is currently unclear.In this study,using bulked segregant RNA sequencing(BSR-seq),a strong candidate Rf gene,Capana06g002866,which is annotated as a NEDD8 conjugating enzyme E2,was identified.Capana06g002866 has an ORF of 555 bp in length encoding 184 amino acids;it can be cloned from F1 plants from the hybridization of the CMS line 8A and restorer line R1 but is not found in CMS line 8A.With qRT-PCR validation,Capana06g002866 was found to be upregulated in restorer accessions compared to sterile accessions.The relative expression in flower buds increased with the developmental stage in F1 plants,while the expression was very low in all flower bud stages of the CMS lines.These results provide new insights into the Rf gene in pepper and will be useful for other crops utilizing the CMS system.展开更多
Pepper(Capsicum spp.)is an important vegetable crop that provides a unique pungent sensation when eaten.Through construction of a pepper variome map,we examined the main groups that emerged during domestication and br...Pepper(Capsicum spp.)is an important vegetable crop that provides a unique pungent sensation when eaten.Through construction of a pepper variome map,we examined the main groups that emerged during domestication and breeding of C.annuum,their relationships and temporal succession,and the molecular events underlying the main transitions.The results showed that the initial differentiation in fruit shape and pungency,increase in fruit weight,and transition from erect to pendent fruits,as well as the recent appearance of large,blocky,sweet fruits(bell peppers),were accompanied by strong selection/fixation of key alleles and introgressions in two large genomic regions.Furthermore,we identified Up,which encodes a BIG GRAIN protein involved in auxin transport,as a key domestication gene that controls erect vs pendent fruit orientation.The up mutation gained increased expression especially in the fruit pedicel through a 579-bp sequence deletion in its 5′upstream region,resulting in the phenotype of pendent fruit.The function of Up was confirmed by virus-induced gene silencing.Taken together,these findings constitute a cornerstone for understanding the domestication and differentiation of a key horticultural crop.展开更多
基金funded by the Scientific Research Start-up Funds for Openly recruited Doctors of Science and Technology Innovation Funds of Gansu Agricultural University,China(GAU-KYQD-2018-17)the National Natural Sciences Foundation of China(31560555,31760572).
文摘Cytoplasmic male sterility(CMS)is an important tool for producing F1 hybrids,which can exhibit heterosis.The companion system,restorer-of-fertility(Rf),is poorly understood at the molecular level and would be valuable in producing restorer lines for hybrid seed production.The identity of the Rf gene in Capsicum(pepper)is currently unclear.In this study,using bulked segregant RNA sequencing(BSR-seq),a strong candidate Rf gene,Capana06g002866,which is annotated as a NEDD8 conjugating enzyme E2,was identified.Capana06g002866 has an ORF of 555 bp in length encoding 184 amino acids;it can be cloned from F1 plants from the hybridization of the CMS line 8A and restorer line R1 but is not found in CMS line 8A.With qRT-PCR validation,Capana06g002866 was found to be upregulated in restorer accessions compared to sterile accessions.The relative expression in flower buds increased with the developmental stage in F1 plants,while the expression was very low in all flower bud stages of the CMS lines.These results provide new insights into the Rf gene in pepper and will be useful for other crops utilizing the CMS system.
基金China National Key Technology Research and Development Program(2016YFD0100200,2016YFD0101700,2018YFD1000800,and 2020YFD1001100)National Natural Science Foundation of China(NSFC grants 31722048,31972411,3170110879,32102382,and 32102386)+5 种基金Central Public-interest Scientific Institution Basal Research Fund(Y2022GH04,IVF-BRF2021015)Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IVFCAAS)Earmarked Fund for the China Agriculture Research System(CARS-25)CAAS-GAAS Coordinated Innovation Project of the Chinese Academy of Agricultural Sciences(2019)Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture and Rural Affairs,P.R.ChinaHorizon 2020 G2P-SOL project(grant no.677379)from the EU.
文摘Pepper(Capsicum spp.)is an important vegetable crop that provides a unique pungent sensation when eaten.Through construction of a pepper variome map,we examined the main groups that emerged during domestication and breeding of C.annuum,their relationships and temporal succession,and the molecular events underlying the main transitions.The results showed that the initial differentiation in fruit shape and pungency,increase in fruit weight,and transition from erect to pendent fruits,as well as the recent appearance of large,blocky,sweet fruits(bell peppers),were accompanied by strong selection/fixation of key alleles and introgressions in two large genomic regions.Furthermore,we identified Up,which encodes a BIG GRAIN protein involved in auxin transport,as a key domestication gene that controls erect vs pendent fruit orientation.The up mutation gained increased expression especially in the fruit pedicel through a 579-bp sequence deletion in its 5′upstream region,resulting in the phenotype of pendent fruit.The function of Up was confirmed by virus-induced gene silencing.Taken together,these findings constitute a cornerstone for understanding the domestication and differentiation of a key horticultural crop.
