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纳他霉素对芒果采后胶孢炭疽菌的抑菌效果及机理 被引量:13
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作者 刘佳怡 王嘉欣 +4 位作者 宋海超 张正科 徐祥彬 吉训聪 史学群 《植物学报》 CAS CSCD 北大核心 2019年第4期455-463,共9页
以纳他霉素为抑菌剂,实验测定了离体条件下不同浓度纳他霉素对胶孢炭疽菌(Colletotrichumgloeosporioides)的孢子萌发及菌丝生长的抑制效果,以及活体损伤接种炭疽病菌后,纳他霉素对芒果(Mangiferaindica)果实炭疽病的防治效果。通过测... 以纳他霉素为抑菌剂,实验测定了离体条件下不同浓度纳他霉素对胶孢炭疽菌(Colletotrichumgloeosporioides)的孢子萌发及菌丝生长的抑制效果,以及活体损伤接种炭疽病菌后,纳他霉素对芒果(Mangiferaindica)果实炭疽病的防治效果。通过测定纳他霉素处理后胶孢炭疽菌的细胞膜相对渗透率、可溶性蛋白含量、细胞膜完整性、孢子内活性氧水平和线粒体分布情况,初步探明其抑菌机理。结果表明, 3 mg·L–1纳他霉素可显著抑制胶孢炭疽菌孢子萌发、芽管伸长和菌落生长,80 mg·L–1纳他霉素可有效抑制芒果贮存过程中果实炭疽病斑的扩展。纳他霉素处理后胶孢炭疽菌细胞膜相对渗透率和可溶性蛋白含量增加;2mg·L–1纳他霉素处理8小时,处理组胶孢炭疽菌孢子细胞膜损伤染色率为33.6%,对照组染色率为13.9%;处理组胞内活性氧产生染色率达46.9%,比对照组高39.7%;同时观察到纳他霉素使胞内线粒体分布不均且荧光信号微弱。以上结果表明,纳他霉素可以破坏胶孢炭疽病菌细胞膜,诱导活性氧大量积累,并降低线粒体活性,从而干扰菌体正常生理活性,使其代谢活动受影响,从而达到抑菌目的。 展开更多
关键词 纳他霉素 芒果 胶孢炭疽病菌 抑菌机理
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芒果胶孢炭疽病菌应答菌丝机械损伤产生无性孢子的分子机制 被引量:1
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作者 王丽妍 卢梦瑶 +5 位作者 童悦 徐祥斌 张正科 孟兰环 史学群 宋海超 《植物学报》 CAS CSCD 北大核心 2020年第5期551-563,共13页
胶孢炭疽菌(Colletotrichum gloeosporioides)是引发芒果(Mangifera indica)炭疽病的主要病原体。室内平板培养胶孢炭疽菌不产生或产生很少分生孢子的情况时有发生,但菌丝在机械损伤后24–48小时会产生大量分生孢子。胶孢炭疽菌应答机... 胶孢炭疽菌(Colletotrichum gloeosporioides)是引发芒果(Mangifera indica)炭疽病的主要病原体。室内平板培养胶孢炭疽菌不产生或产生很少分生孢子的情况时有发生,但菌丝在机械损伤后24–48小时会产生大量分生孢子。胶孢炭疽菌应答机械损伤诱导产孢的核心基因及关键代谢通路尚未见报道。基于转录组测序(RNA-seq)技术检测了芒果胶孢炭疽菌菌丝在机械损伤处理后2小时内5个时间点的基因表达变化,对差异表达基因进行GO富集和KEGG代谢通路富集分析,并对菌丝响应胁迫的基因动态表达数据进行分析。基于常微分方程ODE模型结合变量选择技术,构建了动态基因调控网络。结果表明,有417个差异表达基因参与应答胶孢炭疽菌菌丝机械损伤,分属12个聚类模块,有4条通路存在显著富集,分别是丙酮酸代谢、硫代谢、黄曲霉素合成途径和二萜合成途径。结合功能注释筛选出12个应答菌丝损伤胁迫的核心基因。研究结果为后续深入开展芒果胶孢炭疽菌产孢和致病机理研究奠定了重要基础。 展开更多
关键词 芒果 胶孢炭疽菌 损伤胁迫 RNA-SEQ 动态表达
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Experimental study of an anti-icing method over an airfoil based on pulsed dielectric barrier discharge plasma 被引量:15
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作者 Yongqiang TIAN zhengke zhang +2 位作者 Jinsheng CAI Leilei YANG Lei KANG 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2018年第7期1449-1460,共12页
Aircraft icing has long been a plague to aviation for its serious threat to flight safety. Even though lots of methods for anti-icing have been in use or studied for quite a long time, new methods are still in great d... Aircraft icing has long been a plague to aviation for its serious threat to flight safety. Even though lots of methods for anti-icing have been in use or studied for quite a long time, new methods are still in great demand for both civil and military aircraft. The current study in this paper uses widely used Dielectric Barrier Discharge(DBD) plasma actuation to anti-ice on a NACA0012 airfoil model with a chord length of 53.5 cm in a closed-circuit icing wind tunnel. An actuator was installed at the leading edge of the airfoil model, and actuated by a pulsed low-temperature plasma power source. The actuator has two types of layout, a striped electrode layout and a meshy electrode layout.The ice accretion process or anti-icing process was recorded by a CCD camera and an infrared camera. Instantaneous pictures and infrared contours show that both types of DBD plasma actuators have the ability for anti-ice under a freestream velocity of 90 m/s, a static temperature of -7℃,an Median Volume droplet Diameter(MVD) of 20 lm, and an Liquid Water Content(LWC) of 0.5 g/m^3. The detected variations of temperatures with time at specific locations reveal that the temperatures oscillate for some time after spraying at first, and then tend to be nearly constant values.This shows that the key point of the anti-icing mechanism with DBD plasma actuation is to achieve a thermal equilibrium on the model surface. Besides, the power consumption in the anti-icing process was estimated in this paper by Lissajous figures measured by an oscilloscope, and it is lower than those of existing anti-icing methods. The experimental results presented in this paper indicate that the DBD plasma anti-icing method is a promising technique in the future. 展开更多
关键词 Anti icing Icing wind tunnel Lissajous figures PlaSma actuator Pulsed dielectric barrier discharge
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Response of ammonia-oxidizing archaea to heavy metal contamination in freshwater sediment 被引量:1
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作者 Ningning Li Yao Chen +6 位作者 zhengke zhang Sha Chang Dawei Huang Sili Chen Qingwei Guo Shuguang Xie Yongxin Bing 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2019年第3期392-399,共8页
It has been well-documented that the distribution of ammonia-oxidizing bacteria(AOB) and archaea(AOA) in soils can be affected by heavy metal contamination, whereas information about the impact of heavy metal on these... It has been well-documented that the distribution of ammonia-oxidizing bacteria(AOB) and archaea(AOA) in soils can be affected by heavy metal contamination, whereas information about the impact of heavy metal on these ammonia-oxidizing microorganisms in freshwater sediment is still lacking. The present study explored the change of sediment ammonia-oxidizing microorganisms in a freshwater reservoir after being accidentally contaminated by industrial discharge containing high levels of metals. Bacterial amoA gene was found to be below the quantitative PCR detection and was not successfully amplified by conventional PCR. The number of archaeal amoA gene in reservoir sediments were 9.62 × 10~2–1.35 × 10~7 copies per gram dry sediment. AOA abundance continuously decreased, and AOA richness, diversity and community structure also considerably varied with time. Therefore, heavy metal pollution could have a profound impact on freshwater sediment AOA community. This work could expand our knowledge of the effect of heavy metal contamination on nitrification in natural ecosystems. 展开更多
关键词 Ammonia-oxidizing ARCHAEA FRESHWATER SEDIMENT HEAVY metal Nitrosopumilus
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