[Objectives]This study was conducted to investigate the molecular characteristics,genetic structure and evolutionary mechanism of cucurbit aphid-borne yellows virus(CABYV),which is of great significance for clarifying...[Objectives]This study was conducted to investigate the molecular characteristics,genetic structure and evolutionary mechanism of cucurbit aphid-borne yellows virus(CABYV),which is of great significance for clarifying the epidemic laws of the disease in the field and formulating long-term sustainable control strategies.[Methods]120 melon leaves suspected to be infected with CABYV were randomly collected from Aksu City,Xinjiang.RT-PCR was used to detect the virus.MEGA 7.0 was used to construct a phylogenetic tree.DnaSP v5.10 was used to analyze the genetic diversity among different sequences,and RDP v.4.31 software was used to analyze possible recombination events of CABYV sequence.[Results]The detection rate of CABYV was 31.67%.A new CABYV-2 was isolated,sequenced and cloned from these positive samples.The length of the genome is 5497 bp,encoding six open reading frames.Compared with 23 CABYV strains isolated from different countries in NCBI database,CABYV-2 shared the highest homology with KR231942.1 and the lowest homology with JF939812.1.The results of evolutionary tree analysis showed that the 24 isolates were divided into two branches due to different geographical factors,including CABYV-2 and KR2319421,which were clustered together and belonged to branch 1.The results of genetic diversity and neutrality tests showed that CABYV was highly variable and the population was expanding.The results of recombination analysis showed that there were two recombinants,EU636992.1 and KR231949.1,which exacerbated the variation of CABYV.[Conclusions]The CABYV isolate from Aksu City,Xinjiang had the closest relationship with the Korean isolate KR231942.1,and the farthest relationship with the isolates from European countries.There were large genetic differences between the isolates of branch 1 and branch 2,and the recombination of CABYV in different hosts aggravated the variation of CABYV.Recombination and negative selection may be important reasons for the genetic variation of CABYV.展开更多
基金Supported by National Natural Science Foundation of China(31760511)Linfen Key R&D Program(2009)+1 种基金2020 National Key R&D Program-Technology Helps the Economy 2020 Key Project(2020-NK-ZL34)Fundamental Research Program of Shanxi Province(201901D211403)。
文摘[Objectives]This study was conducted to investigate the molecular characteristics,genetic structure and evolutionary mechanism of cucurbit aphid-borne yellows virus(CABYV),which is of great significance for clarifying the epidemic laws of the disease in the field and formulating long-term sustainable control strategies.[Methods]120 melon leaves suspected to be infected with CABYV were randomly collected from Aksu City,Xinjiang.RT-PCR was used to detect the virus.MEGA 7.0 was used to construct a phylogenetic tree.DnaSP v5.10 was used to analyze the genetic diversity among different sequences,and RDP v.4.31 software was used to analyze possible recombination events of CABYV sequence.[Results]The detection rate of CABYV was 31.67%.A new CABYV-2 was isolated,sequenced and cloned from these positive samples.The length of the genome is 5497 bp,encoding six open reading frames.Compared with 23 CABYV strains isolated from different countries in NCBI database,CABYV-2 shared the highest homology with KR231942.1 and the lowest homology with JF939812.1.The results of evolutionary tree analysis showed that the 24 isolates were divided into two branches due to different geographical factors,including CABYV-2 and KR2319421,which were clustered together and belonged to branch 1.The results of genetic diversity and neutrality tests showed that CABYV was highly variable and the population was expanding.The results of recombination analysis showed that there were two recombinants,EU636992.1 and KR231949.1,which exacerbated the variation of CABYV.[Conclusions]The CABYV isolate from Aksu City,Xinjiang had the closest relationship with the Korean isolate KR231942.1,and the farthest relationship with the isolates from European countries.There were large genetic differences between the isolates of branch 1 and branch 2,and the recombination of CABYV in different hosts aggravated the variation of CABYV.Recombination and negative selection may be important reasons for the genetic variation of CABYV.
