A comprehensive analysis of the humoral immune response to the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is essential in understanding COVID-19 pathogenesis and developing antibody-based diagnostics a...A comprehensive analysis of the humoral immune response to the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is essential in understanding COVID-19 pathogenesis and developing antibody-based diagnostics and therapy.In this work,we performed a longitudinal analysis of antibody responses to SARS-CoV-2 proteins in 104 serum samples from 49 critical COVID-19 patients using a peptide-based SARS-CoV-2 proteome microarray.Our data show that the binding epitopes of IgM and IgG antibodies differ across SARS-CoV-2 proteins and even within the same protein.展开更多
基金This research was supported by grants from the National Key R&D Program of China(2020YFC0861000,2020YFE0202200,2018YFE0207300)Beijing Municipal Science&Technology Commission(Z211100002521021)+4 种基金National Natural Science Foundation of China Grants(81671618,81871302,81673040,31870823)CAMS Innovation Fund for Medical Sciences(CIFMS)(2020-I2M-COV19-001,2017-I2M-3-001,2017-I2M-B&R-01,2019-I2M-5-063)the State Key Laboratory of Proteomics(SKLP-C202001,SKLP-0201703,SKLP-K201505)the Beijing Municipal Education Commission and the National Program on Key Basic Research Project(2018YFA0507503,2017YFC0906703,2018ZX09733003)This work is supported by Beijing Key Clinical Specialty for Laboratory Medicine-Excellent Project(No.ZK201000)。
文摘A comprehensive analysis of the humoral immune response to the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is essential in understanding COVID-19 pathogenesis and developing antibody-based diagnostics and therapy.In this work,we performed a longitudinal analysis of antibody responses to SARS-CoV-2 proteins in 104 serum samples from 49 critical COVID-19 patients using a peptide-based SARS-CoV-2 proteome microarray.Our data show that the binding epitopes of IgM and IgG antibodies differ across SARS-CoV-2 proteins and even within the same protein.
