In this paper,a design method for an acoustic cloak in the presence of background mean flows is proposed by using topology optimization,which enables the associated fabrication of the cloaking design.The density-based...In this paper,a design method for an acoustic cloak in the presence of background mean flows is proposed by using topology optimization,which enables the associated fabrication of the cloaking design.The density-based topology optimization method is used to allocate the designated materials,thus providing the structure of the cloak.The optimization problem is efficiently solved with the gradient-based globally convergent method of moving asymptotes,which utilizes the derivative information from the finite element simulation studies of the linearized acoustic potential equation.This paper introduces the whole design method first then numerically demonstrates the corresponding performance,which shall constitute the main contribution of the present work.展开更多
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a new member of TNF family. It was reported that TRAIL could induce apoptosis of tumor cells but not normal cells in tissue culture system. To f...Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a new member of TNF family. It was reported that TRAIL could induce apoptosis of tumor cells but not normal cells in tissue culture system. To further study the biological activity and potential clinical significance, a recombinant soluble TRAIL (rsTRAIL) has been expressed stably in E. coli after transformation of pET28b vector containing the extracellular domain of TRAIL. The yield of rsTRAIL is approximately as high as 60% of whole bacterial proteins. The rsTRAIL, purified by Ni^+ -agarose affinity chromatography, could remarkably trigger apoptosis at the concentrations of 0.1—1 μg/mL in all 7 tumor cell lines tested in vitro. However, this killing activity has not been observed in mouse fibroblast cell line (NIH3T3) as normal control. Further investigation shows that the rsTRAIL could also kill primary tumor cells isolated freshly from patients with cardiac cancer, breast cancer and malignant thymoma, while the normal human展开更多
Active exploratory behaviors have often been associated with theta oscillations in rodents,while theta oscillations during active exploration in non-human primates are still not well understood.We recorded neural acti...Active exploratory behaviors have often been associated with theta oscillations in rodents,while theta oscillations during active exploration in non-human primates are still not well understood.We recorded neural activities in the frontal eye field(FEF)and V4 simultaneously when monkeys performed a free-gaze visual search task.Saccades were strongly phase-locked to theta oscillations of V4 and FEF local field potentials,and the phase-locking was dependent on saccade direction.The spiking probability of V4 and FEF units was significantly modulated by the theta phase in addition to the time-locked modulation associated with the evoked response.V4 and FEF units showed significantly stronger responses following saccades initiated at their preferred phases.Granger causality and ridge regression analysis showed modulatory effects of theta oscillations on saccade timing.Together,our study suggests phase-locking of saccades to the theta modulation of neural activity in visual and oculomotor cortical areas,in addition to the theta phase locking caused by saccade-triggered responses.展开更多
We present P(TMC-co-DLLA)copolymer with the molar ratio of TMC:DLLA紏15:85 was used to systematic study of in vivo and in vitro degradation behaviors.Dense homogeneous copolymer specimens were prepared by compression ...We present P(TMC-co-DLLA)copolymer with the molar ratio of TMC:DLLA紏15:85 was used to systematic study of in vivo and in vitro degradation behaviors.Dense homogeneous copolymer specimens were prepared by compression molding method.The in vitro and in vivo degradation were,respectively,performed at simulative body condition and implanted into rat’s subcutaneous condition.Investigations were followed via physicochemical and histological analysis such as SEM,GPC,DSC,FTIR and H&E stain.The results demonstrate that copolymeric material can degrade in phosphate buffer solution(PBS)and in rat’s body,and the in vivo degradation rate is faster.Obvious decline of molecule weight and mass loss has been observed,which led to the attenuation of mechanical strength.Furthermore,apart from the hydrolysis,macrophagocytes took part in the phagocytosis in vivo,indicating that degradation rate could be regulated by the combinational mechanism.It is concluded that P(TMC-co-DLLA)copolymer is a promising candidate for tissue repair.展开更多
基金This work was supported by the Beijing Municipal Science&Technology Commission(Grant Z181100001018030)the National Natural Science Foundation of China(Grant 11561130148)the Newton Advanced Fellowship from the Royal Society(Ref.NA14081).
文摘In this paper,a design method for an acoustic cloak in the presence of background mean flows is proposed by using topology optimization,which enables the associated fabrication of the cloaking design.The density-based topology optimization method is used to allocate the designated materials,thus providing the structure of the cloak.The optimization problem is efficiently solved with the gradient-based globally convergent method of moving asymptotes,which utilizes the derivative information from the finite element simulation studies of the linearized acoustic potential equation.This paper introduces the whole design method first then numerically demonstrates the corresponding performance,which shall constitute the main contribution of the present work.
文摘Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a new member of TNF family. It was reported that TRAIL could induce apoptosis of tumor cells but not normal cells in tissue culture system. To further study the biological activity and potential clinical significance, a recombinant soluble TRAIL (rsTRAIL) has been expressed stably in E. coli after transformation of pET28b vector containing the extracellular domain of TRAIL. The yield of rsTRAIL is approximately as high as 60% of whole bacterial proteins. The rsTRAIL, purified by Ni^+ -agarose affinity chromatography, could remarkably trigger apoptosis at the concentrations of 0.1—1 μg/mL in all 7 tumor cell lines tested in vitro. However, this killing activity has not been observed in mouse fibroblast cell line (NIH3T3) as normal control. Further investigation shows that the rsTRAIL could also kill primary tumor cells isolated freshly from patients with cardiac cancer, breast cancer and malignant thymoma, while the normal human
基金We are grateful for the financial support from the National Natural Science Foundation of China(31671108 and 31800900)the National Key R&D Program of China(2017YFC1307500)+1 种基金the Shenzhen Science and Technology Innovation Commission(JCYJ20180508152240368)the Shenzhen Basic Research Program(JCYJ20200109114805984).
文摘Active exploratory behaviors have often been associated with theta oscillations in rodents,while theta oscillations during active exploration in non-human primates are still not well understood.We recorded neural activities in the frontal eye field(FEF)and V4 simultaneously when monkeys performed a free-gaze visual search task.Saccades were strongly phase-locked to theta oscillations of V4 and FEF local field potentials,and the phase-locking was dependent on saccade direction.The spiking probability of V4 and FEF units was significantly modulated by the theta phase in addition to the time-locked modulation associated with the evoked response.V4 and FEF units showed significantly stronger responses following saccades initiated at their preferred phases.Granger causality and ridge regression analysis showed modulatory effects of theta oscillations on saccade timing.Together,our study suggests phase-locking of saccades to the theta modulation of neural activity in visual and oculomotor cortical areas,in addition to the theta phase locking caused by saccade-triggered responses.
基金The authors would like to express their gratitude to the National Natural Science Foundation of China(No.31330028,31470923,31271011)National Basic Research Program of China(973 Program,No.2012CB933600),the 111 Project(B14018)+1 种基金New Century Excellent Talents in University(No.NCET-12-0856)This study is also supported by Major Basic Research Foundation of Shanghai Science and Technique Committee(14JC1490800).
文摘We present P(TMC-co-DLLA)copolymer with the molar ratio of TMC:DLLA紏15:85 was used to systematic study of in vivo and in vitro degradation behaviors.Dense homogeneous copolymer specimens were prepared by compression molding method.The in vitro and in vivo degradation were,respectively,performed at simulative body condition and implanted into rat’s subcutaneous condition.Investigations were followed via physicochemical and histological analysis such as SEM,GPC,DSC,FTIR and H&E stain.The results demonstrate that copolymeric material can degrade in phosphate buffer solution(PBS)and in rat’s body,and the in vivo degradation rate is faster.Obvious decline of molecule weight and mass loss has been observed,which led to the attenuation of mechanical strength.Furthermore,apart from the hydrolysis,macrophagocytes took part in the phagocytosis in vivo,indicating that degradation rate could be regulated by the combinational mechanism.It is concluded that P(TMC-co-DLLA)copolymer is a promising candidate for tissue repair.
