Reliability and Validity of the Chinese Version of the Lymphedema Quality of Life Questionnaire

Objective To study the reliability and validity of the Chinese version of the Lymphedema Quality of Life Questionnaire(LYMQOL)in lymphedema patients.Methods LYMQOL was translated into Chinese.The Chinese version of the LYMQOL was distributed with the official Wechat account"Lymphedema Channel"to lymphedema patients who were recruited from October 28^(th),2020 to February 23^(rd),2021.Patients with upper limb lymphedema and lower limb lymphedema completed the LYMQOL-ARM subscale and the LYMQOL-LEG subscale separately,at enrollment,1 week later,and 1 month later.Reliability,validity,feasibility,responsiveness and average time required for completing the questionnaire were assessed.Results A total of 195 patients participated in the study.The Chinese questionnaire showed high reliability with Cronbach’sαcoefficients of 0.849-0.902 for the LYMQOL-ARM and intraclass correlation coefficient(ICC)of 0.848-0.884 and Cronbach’sαcoefficients of 0.726-0.902 for the LYMQOL-LEG and ICC of 0.863-0.900.The LYMQOL showed moderate to good correlations with the EQ-5 D(0.4<r<0.6,P<0.01;for the LYMQOLARM,n=95,for the LYMQOL-LEG,n=102).Responsiveness analysis suggested that quality of life of upper limb lymphedema patients decreased after 1 month(P<0.05).The average time of patients to finish the questionnaire was approximately 12 minutes.Conclusions The Chinese version of the LYMQOL is easy to answer,comprehensive and appropriate in length,and has good reliability and validity.It may be utilized to assist treatment decision-making and track changes in clinical setting or research for lymphedema.

Decoding the molecular landscape of keloids:new insights from single-cell transcriptomics

Keloids are a fibrotic disease caused by an excessive accumulation of extracellular matrix in the dermis;they have neoplasia-like properties of aggressive growth and high posttreatment recurrence rates.Therefore,it is imperative to gain additional insight into the pathobiology of keloid formation.Single-cell RNA sequencing(scRNA-seq)technology has brought data-driven innovation to understanding the pathogenesis of keloids by breaking the limitations of traditional sequencing technologies to resolve cell composition and to distinguish functional cell subtypes at an unprecedented resolution.The present review aims to cover the application of scRNAseq technology in keloids and its exploratory findings,including the depiction of the cellular landscape of keloids,fibroblast heterogeneity,the lineage development of Schwann cells and the mesenchymal-activation phenomenon of endothelial cells.Furthermore,scRNA-seq records the transcriptional profiles of fibroblasts and immune cells in a more refined manner,and this gene expression information provides excellent material for inferring intercellular communication networks and lays an important theoretical foundation for future studies.

Advances in the pathogenesis and clinical application prospects of tumor biomolecules in keloid

Keloid scarring is a kind of pathological healing manifestation after skin injury and possesses various tumor properties,such as the Warburg effect,epithelial-mesenchymal transition(EMT),expression imbalances of apoptosis-related genes and the presence of stem cells.Abnormal expression of tumor signatures is critical to the initiation and operation of these effects.Although previous experimental studies have recognized the potential value of a single or several tumor biomolecules in keloids,a comprehensive evaluation system for multiple tumor signatures in keloid scarring is still lacking.This paper aims to summarize tumor biomolecules in keloids from the perspectives of liquid biopsy,genetics,proteomics and epigenetics and to investigate their mechanisms of action and feasibility from bench to bedside.Liquid biopsy is suitable for the early screening of people with keloids due to its noninvasive and accurate performance.Epigenetic biomarkers do not require changes in the gene sequence and their reversibility and tissue specificity make them ideal therapeutic targets.Nonetheless,given the ethnic specificity and genetic predisposition of keloids,more large-sample multicenter studies are indispensable for determining the prevalence of these signatures and for establishing diagnostic criteria and therapeutic efficacy estimations based on these molecules.