[Objectives]To investigate the expression and significance of miR-21 and P-gp in colon cancer cell line HCT-15.[Methods]RT-qPCR was used to detect the miR-21 and ABCB1 mRNA expression level of P-gp coding genes in nor...[Objectives]To investigate the expression and significance of miR-21 and P-gp in colon cancer cell line HCT-15.[Methods]RT-qPCR was used to detect the miR-21 and ABCB1 mRNA expression level of P-gp coding genes in normal colon mucosal cell NCM460 and colon cancer cell HCT-15,and Western Blot was used to detect the expression of P-gp in these two cell lines.[Results]The results of RT-qPCR showed that the expression level of miR-21 and ABCB1 mRNA in colon cancer cell HCT-15 was significantly higher than that in normal colon cell NCM460,and the difference was statistically significant(P<0.05).Western Blot results showed that the expression level of P-gp in HCT-15 was significantly higher than that in NCM460,and the difference was statistically significant(P<0.05).[Conclusions]The expression level of miR-21 and ABCB1 mRNA/P-gp in colon cancer cell HCT-15 was higher than that in normal colonic mucosal cell NCM460,and the relationship between miR-21 and multidrug resistance in colon cancer might be related to P-gp.展开更多
基金Provincial-level Project of Hebei of Priority Discipline Construction(031412)School-level Natural Fund Project of Chengde Medical University(201830).
文摘[Objectives]To investigate the expression and significance of miR-21 and P-gp in colon cancer cell line HCT-15.[Methods]RT-qPCR was used to detect the miR-21 and ABCB1 mRNA expression level of P-gp coding genes in normal colon mucosal cell NCM460 and colon cancer cell HCT-15,and Western Blot was used to detect the expression of P-gp in these two cell lines.[Results]The results of RT-qPCR showed that the expression level of miR-21 and ABCB1 mRNA in colon cancer cell HCT-15 was significantly higher than that in normal colon cell NCM460,and the difference was statistically significant(P<0.05).Western Blot results showed that the expression level of P-gp in HCT-15 was significantly higher than that in NCM460,and the difference was statistically significant(P<0.05).[Conclusions]The expression level of miR-21 and ABCB1 mRNA/P-gp in colon cancer cell HCT-15 was higher than that in normal colonic mucosal cell NCM460,and the relationship between miR-21 and multidrug resistance in colon cancer might be related to P-gp.
