AbnI:Anα-ketoglutarate-dependent dioxygenase involved in brassicicene C-H functionalization and ring system rearrangement

We reported the characterization of a novel brassicicene diterpene biosynthetic gene cluster,which contains a uniqueα-ketoglutarate-dependent dioxygenase(αKGD)enzyme,AbnI.Our findings revealed that AbnI demonstrates remarkable substrate promiscuity and is capable of activating multiple sites on both 5-8-5 and 5-9-5 brassicicene skeletons,resulting in skeleton modifications and an unexpected ring system rearrangement.These results suggested the potential utility of AbnI as an enzymatic tool for terpene C-H functionalization.In addition,the catalytic mechanism of AbnI and its potential ecological implications were discussed.

被动声学监测技术在西黑冠长臂猿监测中的应用

近年来,被动声学监测技术被广泛应用于陆生哺乳动物的监测,它能以较低的价格和非侵入的方式在特定区域进行野生动物无人值守监测,面临的主要问题是需要人工收回数据和后期数据分析整理较为困难。本研究设计了一套被动声学监测系统用于西黑冠长臂猿(Nomascusconcolor)监测,监测系统在野外由太阳能供电,使用自研的指向性拾音器阵列采集鸣声数据,并通过无线网桥实时传输数据至管护局办公楼的服务器进行存储,通过后台的鸣声数据管理系统辅助研究人员识别鸣声和辨认鸣声来源方向,简化数据采集和处理流程。该系统在哀牢山国家级自然保护区枇杷箐科研监听点对两个西黑冠长臂猿群体进行了351天的连续监测,特点为:系统运行长期稳定,数据通过无线方式传输便捷高效且不受季节天气影响。指向性拾音器阵列能有效分辨长臂猿鸣声来源方向,弥补了传统监测设备难以分辨鸣声方向的缺陷。该系统与现有人工监测方法相比在数据采集的持续性、连续性、完整度以及鸣声数据处理智能化和监测成本方面均具有一定优势,符合西黑冠长臂猿持续长期监测需求,未来可作为西黑冠长臂猿自动化监测的解决方案进行推广应用。