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PpERF3 positively regulates ABA biosynthesis by activating PpNCED2/3 transcription during fruit ripening in peach 被引量:10
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作者 Xiaobei Wang Wenfang Zeng +8 位作者 Yifeng Ding Yan Wang Liang Niu Jia-Long Yao Lei Pan zhenhua lu Guochao Cui Guohuai Li Zhiqiang Wang 《Horticulture Research》 SCIE 2019年第1期1535-1544,共10页
The plant hormone ethylene regulates ripening in climacteric fruits.The phytohormone abscisic acid(ABA)affects ethylene biosynthesis,but whether ethylene influences ABA biosynthesis is unknown.To explore this possibil... The plant hormone ethylene regulates ripening in climacteric fruits.The phytohormone abscisic acid(ABA)affects ethylene biosynthesis,but whether ethylene influences ABA biosynthesis is unknown.To explore this possibility,we investigated the interactions between the ABA biosynthesis genes PpNCED2/3 and the ethylene response transcription factor PpERF3 in peach fruit.The ABA content increased during fruit maturation and reached a peak at stage S4 III.The increase was greatly inhibited by the ethylene inhibitor 1-MCP,which also suppressed PpERF3 expression.PpERF3 shared a similar expression profile with PpNCED2/3,encoding a rate-limiting enzyme involved in ABA biosynthesis,during fruit ripening.A yeast one-hybrid assay suggested that the nuclear-localized PpERF3 might bind to the promoters of PpNCED2/3.PpERF3 increased the expression of PpNCED2/3 as shown by dual-luciferase reporters,promoter-GUS assays and transient expression analyses in peach fruit.Collectively,these results suggest that ethylene promotes ABA biosynthesis through PpERF3’s regulation of the expression of ABA biosynthesis genes PpNCED2/3. 展开更多
关键词 ERF LIMITING fruit
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Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model 被引量:2
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作者 Wenfang Zeng Liang Niu +9 位作者 Zhaohui Wang Xiaobei Wang Yan Wang Lei Pan zhenhua lu Guochao Cui Weining Weng Mingqiao Wang Xun Meng Zhiqiang Wang 《Horticulture Research》 SCIE 2020年第1期2328-2341,共14页
Peach(Prunus persica)is a typical climacteric fruit that produces ethylene rapidly during ripening,and its fruit softens quickly.Stony hard peach cultivars,however,do not produce large amounts of ethylene,and the frui... Peach(Prunus persica)is a typical climacteric fruit that produces ethylene rapidly during ripening,and its fruit softens quickly.Stony hard peach cultivars,however,do not produce large amounts of ethylene,and the fruit remains firm until fully ripe,thus differing from melting flesh peach cultivars.To identify the key proteins involved in peach fruit ripening,an antibody-based proteomic analysis was conducted.A mega-monoclonal antibody(mAb)library was generated and arrayed on a chip(mAbArray)at a high density,covering~4950 different proteins of peach.Through the screening of peach fruit proteins with the mAbArray chip,differentially expressed proteins recognized by 1587 mAbs were identified,and 33 corresponding antigens were ultimately identified by immunoprecipitation and mass spectrometry.These proteins included not only important enzymes involved in ethylene biosynthesis,such as ACO1,SAHH,SAMS,and MetE,but also novel factors such as NUDT2.Furthermore,protein–protein interaction analysis identified a metabolon containing SAHH and MetE.By combining the antibody-based proteomic data with the transcriptomic and metabolic data,a mathematical model of ethylene biosynthesis in peach was constructed.Simulation results showed that MetE is an important regulator during peach ripening,partially through interaction with SAHH. 展开更多
关键词 PEACH CYCLE SCREENING
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比较肿瘤性与非肿瘤性机械性肠梗阻的临床诊治效果 被引量:1
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作者 陆振华 谢连和 张琳 《临床检验杂志(电子版)》 2016年第3期123-125,共3页
目的比较肿瘤性与非肿瘤性机械性肠梗阻的临床诊治效果。方法选取2013年2月-2015年2月于我院治疗的180例机械性肠梗阻患者为研究对象,对其临床资料进行回顾性分析,其中67例肿瘤性肠梗阻患者为肿瘤组,113例非肿瘤性肠梗阻患者为非肿瘤组... 目的比较肿瘤性与非肿瘤性机械性肠梗阻的临床诊治效果。方法选取2013年2月-2015年2月于我院治疗的180例机械性肠梗阻患者为研究对象,对其临床资料进行回顾性分析,其中67例肿瘤性肠梗阻患者为肿瘤组,113例非肿瘤性肠梗阻患者为非肿瘤组。比较两组患者的诊疗效果。结果肿瘤组患者均行手术治疗,手术方式主要为肠切除术或肠切除+造瘘术;非肿瘤组中,有56例行非手术治疗,57例行手术治疗,手术方式主要为肠切除术或粘连松解术;肿瘤组患者治疗总有效率为76.12%,低于非肿瘤组的81.42%,但差异无统计学意义(P>0.05)。结论手术治疗是临床治疗肿瘤性肠梗阻的首选治疗方法,而非肿瘤肠梗阻患者则需要根据临床情况合理选择治疗方法,这对提高治疗效果、改善预后具有重要意义。 展开更多
关键词 机械性肠梗阻 诊断 治疗
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Diagnosing and reintegrating traceability of infectious diseases via metagenomic next-generation sequencing:Study of a severe case of Rickettsia japonica infection
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作者 zhenhua lu Jing Han +4 位作者 Yuanyuan Wang Zhen He Shuxuan Song Kun Liu Zhongjun Shao 《Infectious Medicine》 2024年第1期79-83,共5页
Background:In this study,we present a case of Japanese spotted fever(JSF)caused by Rickettsia japonica and use this case to investigate the process of diagnosing and reintegrating traceability of infectious diseases v... Background:In this study,we present a case of Japanese spotted fever(JSF)caused by Rickettsia japonica and use this case to investigate the process of diagnosing and reintegrating traceability of infectious diseases via metagenomic next-generation sequencing(mNGS).Methods:From data relating to epidemiological history,clinical and laboratory examinations,and mNGS se-quencing,a diagnosis of severe JSF was concluded.Results:A detailed field epidemiological investigation discovered parasitic Haemaphysalis longicornis from a host animal(dog)in the domicile of the patient,within which R.japonica was detected,along with a diverse array of other potentially pathogenic microorganisms that could cause other infectious diseases.Conclusion:The mNGS provided an efficient method to diagnose JSF infection.This methodology could also be applied to field epidemiological investigations to establish the traceability of infectious diseases. 展开更多
关键词 Rickettsial disease Japanese spotted fever mNGS Field epidemiological investigation
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昆山市急救志愿者培训成果转化现状分析及管理方法研究
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作者 陆振华 陶秀萍 《中华卫生应急电子杂志》 2024年第3期160-164,共5页
目的分析昆山市急救中心培训科通过招募急救志愿者的方式提高急救公益性培训成果转化的可行性与成效,探讨一种可持续应用的管理方法。方法对2018年至2023年志愿者信息进行统计。应用SPSS21软件对志愿者一般情况、成果转化情况、志愿者... 目的分析昆山市急救中心培训科通过招募急救志愿者的方式提高急救公益性培训成果转化的可行性与成效,探讨一种可持续应用的管理方法。方法对2018年至2023年志愿者信息进行统计。应用SPSS21软件对志愿者一般情况、成果转化情况、志愿者培训成果转化管理路径及成效进行数据统计和描述性分析。结果志愿者招募人数逐年增加,完成志愿服务次数逐年增加,培训成果转化率高,转化形式表现为宣传册、媒体宣传、微信公众号、广场宣传、网课培训,社会评价高;在志愿者接受志愿服务调查结果中,59.79%支持全年服务,因家庭(15.47%)、工作(19.59%)、个人(5.15%)原因暂停服务人数占40.21%;在参加志愿者意愿调查中,35.05%为了学习急救知识,28.87%为精神信仰,19.59%受他人影响,16.49%服务社会;在培训科开展志愿者培训成果转化管理中,效果评估与培训保障是管理的亮点,促进培训成果转化。结论培训科在提升急救公益培训成果转化管理中选定急救志愿者为目标受众,管理路径中完成受训者、工作环境、组织三个层面的培训成果转化引领,加强观念障碍和执行障碍管理,注重培训全过程的转化准备,激发转化动机、提供转化机会、控制转化结果,管理思路清晰,管理目标明确,管理方法严谨,可操作性强,适用于急救公益性培训活动管理。 展开更多
关键词 院前急救 志愿者 培训保障 培训成果转化
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