Human umbilical cord blood was collected from full-term deliveries scheduled for cesarean section. Mononuclear cells were isolated, amplified and induced as mesenchymal stem cells. Isolated mesenchymal stem cells test...Human umbilical cord blood was collected from full-term deliveries scheduled for cesarean section. Mononuclear cells were isolated, amplified and induced as mesenchymal stem cells. Isolated mesenchymal stem cells tested positive for the marker CD29, CD44 and CD105 and negative for typical hematopoietic and endothelial markers. Following treatment with neural induction medium containing brain-derived neurotrophic factor for 7 days, the adherent cells exhibited neuron-like cellular morphology. Immunohistochemical staining and reverse transcription-PCR revealed that the induced mesenchymal stem cells expressed the markers for neuron-specific enolase and neurofilament. The results demonstrated that human umbilical cord blood-derived mesenchymal stem cells can differentiate into neuron-like cells induced by brain-derived neurotrophic factor in vitro.展开更多
Objective:allergic granulomatous vas-culitis is relatively rare in clinic.We aimed to analyze the clinical characteristics of allergic granulomatous vasculi-tis.Methods:collecting allergic granulomatous vasculitis pat...Objective:allergic granulomatous vas-culitis is relatively rare in clinic.We aimed to analyze the clinical characteristics of allergic granulomatous vasculi-tis.Methods:collecting allergic granulomatous vasculitis patients in Taihe Hospital from January 2017 to June 2020,sorting out their general information,system involvement and clinical examination results,and analyzing their clini-cal characteristics.Results:a total of 5 patients diagnosed with allergic granulomatous vasculitis were collected,all with multiple organ involvement,including the skin,re-spiratory system,circulatory system,digestive system,urinary system,and nervous system.Respiratory system involvement was mainly due to decreased lung function;60%manifested as obstructive ventilatory dysfunction.Lung biopsy showed a large amount of eosinophil infil-tration in the interstitium of the alveolar septum,and eo-sinophil and fibrin-like exudation in the alveolar cavity.The circulatory system was mainly affected by myocardi-al damage,and patient two(P2)had pericardial effusion.60%of nervous system involvement was central nervous system involvement,and 40%was peripheral nerve in-volvement.Skin system involvement accounted for 60%.Urinary system involvement was mainly manifested as hematuria and proteinuria.Digestive system involvement was mainly manifested as gastritis,gastric ulcer and other diseases.Conclusion:allergic granulomatous vasculitis is clinically rare.The clinical manifestations are atypical and often involve damage to multiple systems.Clinicians need to fully understand the characteristics of the disease and make comprehensive judgments based on the onset process and examination results to avoid misdiagnosis.展开更多
基金the National Basic Research Program of China(973 Program),No. 2005CB522604
文摘Human umbilical cord blood was collected from full-term deliveries scheduled for cesarean section. Mononuclear cells were isolated, amplified and induced as mesenchymal stem cells. Isolated mesenchymal stem cells tested positive for the marker CD29, CD44 and CD105 and negative for typical hematopoietic and endothelial markers. Following treatment with neural induction medium containing brain-derived neurotrophic factor for 7 days, the adherent cells exhibited neuron-like cellular morphology. Immunohistochemical staining and reverse transcription-PCR revealed that the induced mesenchymal stem cells expressed the markers for neuron-specific enolase and neurofilament. The results demonstrated that human umbilical cord blood-derived mesenchymal stem cells can differentiate into neuron-like cells induced by brain-derived neurotrophic factor in vitro.
文摘Objective:allergic granulomatous vas-culitis is relatively rare in clinic.We aimed to analyze the clinical characteristics of allergic granulomatous vasculi-tis.Methods:collecting allergic granulomatous vasculitis patients in Taihe Hospital from January 2017 to June 2020,sorting out their general information,system involvement and clinical examination results,and analyzing their clini-cal characteristics.Results:a total of 5 patients diagnosed with allergic granulomatous vasculitis were collected,all with multiple organ involvement,including the skin,re-spiratory system,circulatory system,digestive system,urinary system,and nervous system.Respiratory system involvement was mainly due to decreased lung function;60%manifested as obstructive ventilatory dysfunction.Lung biopsy showed a large amount of eosinophil infil-tration in the interstitium of the alveolar septum,and eo-sinophil and fibrin-like exudation in the alveolar cavity.The circulatory system was mainly affected by myocardi-al damage,and patient two(P2)had pericardial effusion.60%of nervous system involvement was central nervous system involvement,and 40%was peripheral nerve in-volvement.Skin system involvement accounted for 60%.Urinary system involvement was mainly manifested as hematuria and proteinuria.Digestive system involvement was mainly manifested as gastritis,gastric ulcer and other diseases.Conclusion:allergic granulomatous vasculitis is clinically rare.The clinical manifestations are atypical and often involve damage to multiple systems.Clinicians need to fully understand the characteristics of the disease and make comprehensive judgments based on the onset process and examination results to avoid misdiagnosis.