Fungi are well known for production of antibiotics and other bioactive secondary metabolites,that can be served as pharmaceuticals,therapeutic agents and industrially useful compounds.However,compared with the charact...Fungi are well known for production of antibiotics and other bioactive secondary metabolites,that can be served as pharmaceuticals,therapeutic agents and industrially useful compounds.However,compared with the characterization of prokaryotic biosynthetic gene clusters(BGCs),less attention has been paid to evaluate fungal BGCs.This is partially because heterologous expression of eukaryotic gene constructs often requires replacement of original promoters and terminators,as well as removal of intron sequences,and this substantially slow down the workflow in natural product discovery.It is therefore of interest to investigate the possibility and effectiveness of heterologous expression and library screening of intact BGCs without refactoring in industrial friendly microbial cell factories,such as the yeast Saccharomyces cerevisiae.Here,we discuss the importance of developing new research directions on library screening of fungal BGCs in yeast without refactoring,followed by outlooking prominent opportunities and challenges for future advancement.展开更多
基金This work was supported by National Key Research and Development Program(2018YFA0901800)National Natural Science Foundation of China(22078012)+1 种基金Chinese Postdoctoral Science Foundation(2020M670115)the Novo Nordisk Foundation(NNF10CC1016517),the Knut and Alice Wallenberg Foundation and Beijing Advanced Innovation Center for Soft Matter Science and Engineering.
文摘Fungi are well known for production of antibiotics and other bioactive secondary metabolites,that can be served as pharmaceuticals,therapeutic agents and industrially useful compounds.However,compared with the characterization of prokaryotic biosynthetic gene clusters(BGCs),less attention has been paid to evaluate fungal BGCs.This is partially because heterologous expression of eukaryotic gene constructs often requires replacement of original promoters and terminators,as well as removal of intron sequences,and this substantially slow down the workflow in natural product discovery.It is therefore of interest to investigate the possibility and effectiveness of heterologous expression and library screening of intact BGCs without refactoring in industrial friendly microbial cell factories,such as the yeast Saccharomyces cerevisiae.Here,we discuss the importance of developing new research directions on library screening of fungal BGCs in yeast without refactoring,followed by outlooking prominent opportunities and challenges for future advancement.
