期刊文献+

二次检索

题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

年份

学科

期刊

共找到1篇文章
< 1 >
每页显示 20 50 100
A novel Lactococcus lactis L-arabinose isomerase for D-tagatose production from lactose
1
作者 Susu Zhang zhenshang xu +4 位作者 Ming Ma Guoyan Zhao Runlei Chang Hongli Si Meixue Dai 《Food Bioscience》 SCIE 2022年第4期339-348,共10页
A new strain of Lactococcus lactis producing D-tagatose was isolated and identified.Its L-arabinose isomerase coding gene was cloned and expressed in Escherichia coli BL21(DE3).The optimal temperature and pH of the pu... A new strain of Lactococcus lactis producing D-tagatose was isolated and identified.Its L-arabinose isomerase coding gene was cloned and expressed in Escherichia coli BL21(DE3).The optimal temperature and pH of the purified enzyme were 50◦C and 8.0.To produce D-tagatose from lactose,β-D-galactosidases from Lc.Lactis,Lactiplantibacillus plantarum,and Streptococcus thermophilus were further incorporated into E.coli by two strategies.Theseβ-D-galactosidases were fused to L-arabinose isomerase coupled with a peptide linker(GGGGS)3.Meanwhile,they were co-expressed with L-arabinose isomerase using pETDuet-1 vector.Among these recombinant strains,the cell co-expressing L-arabinose isomerase and S.thermophilusβ-D-galactosidase showed maximal activity.SDS-PAGE results confirmed that exogenous enzymes had the maximum soluble expression level in this strain.At the optimal condition,the conversion rate of D-tagatose from 300 g/L lactose achieved to 42.4%,and the volumetric productivity reached 4.28 g/L/h at 15 h.This research established a highly efficient biotransformation system to produce D-tagatose from lactose. 展开更多
关键词 Lactose D-tagatose Lactic acid bacteria L-arabinose isomerase β-D-galactosidase
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部