Major ozonated autohemotherapy is classically used in treating ischemic disorder of the lower limbs In the present study, we performed major ozonated autohemotherapy treatment in patients with acute cerebral infarctio...Major ozonated autohemotherapy is classically used in treating ischemic disorder of the lower limbs In the present study, we performed major ozonated autohemotherapy treatment in patients with acute cerebral infarction, and assessed outcomes according to the U.S. National Institutes of Health Stroke Score, Modified Rankin Scale, and transcranial magnetic stimulation motor-evoked potential. Compared with the control group, the clinical total effective rate and the cortical potential rise rate of the upper limbs were significantly higher, the central motor conduction time of upper limb was significantly shorter, and the upper limb motor-evoked potential amplitude was significantly increased, in the ozone group. In the ozone group, the National Institutes of Health Stroke Score was positively correlated with the central motor conduction time and the motor-evoked potential amplitude of the upper limb. Central motor conduction time and motor-evoked potential amplitude of the upper limb may be effective indicators of motor-evoked potentials to assess upper limb motor function in cerebral infarct patients. Furthermore, major ozonated autohemotherapy may promote motor function recovery of the upper limb in patients with acute cerebral infarction.展开更多
Xiaoyan 60(XY60) is a new wheat variety bred in the laboratory of Zhensheng Li.After salt treatment, seedlings of XY60 maintain green leaves and produce longer roots than the high yielding cultivar Zhongmai 175(ZM175)...Xiaoyan 60(XY60) is a new wheat variety bred in the laboratory of Zhensheng Li.After salt treatment, seedlings of XY60 maintain green leaves and produce longer roots than the high yielding cultivar Zhongmai 175(ZM175).To explain these different phenotypes we carried out an RNA-Seq analysis using 12 samples from three tissues of both varieties subjected to salt and control treatments.By comparing data from the salt treated plants with the control, 703, 979, and 1197 differentially expressed genes(DEGs) were detected in new leaves, old leaves, and roots of XY60, respectively.The corresponding numbers for ZM175 were 613, 1401, and 1301.The most significantly enriched Gene Ontology(GO) terms and KEGG pathways were associated with polyunsaturated fatty acid(PUFA) metabolism in both new and old leaves from XY60.They were associated with photosynthesis and energy metabolism in ZM175.The most significantly enriched KEGG pathway in roots of both varieties was “glucosinolate biosynthesis”.In addition, jasmonic acid(JA) concentration in XY60 was higher than in ZM175, although it increased significantly in both varieties following salt treatment.Trends in relative expression levels of AOS, MYC2, and JAZ revealed by qRT-PCR were concordant with those from RNA-Seq.Our results suggest that PUFAs may contribute to salt tolerance in common wheat by enhancing the photosynthetic system and JA-related pathways.展开更多
目的:探讨身体质量指数(body mass index,BMI)与放疗前、后乳腺癌相关淋巴水肿(breast cancer related lymphedema,BCRL)的相关性。方法:分析2013年11月至2015年2月281例于河北医科大学第四医院收治的单侧乳腺癌术后女性患者的前瞻性临...目的:探讨身体质量指数(body mass index,BMI)与放疗前、后乳腺癌相关淋巴水肿(breast cancer related lymphedema,BCRL)的相关性。方法:分析2013年11月至2015年2月281例于河北医科大学第四医院收治的单侧乳腺癌术后女性患者的前瞻性临床资料,根据BMI<25、25~27、BMI≥28分为BMI低组(n=94)、中组(n=89)、高组(n=98),比较健患侧上肢体积差(upper limb volume difference,ULVD),采用广义估计方程(GEE)模型和线性逻辑回归模型进行单因素和多因素分析,评估放疗对BCRL(定义为ULVD≥200 m L)的影响,并与其他BMI分界值分组结果进行比较。结果:放疗前、后ULVD平均值分别为40.6、42.9 m L,中位值均为30.0 m L,二者之间差异无统计学意义(P>0.05)。放疗前(2例缺失)BMI低、中、高组的BCRL发生率分别为2.2%(2/93)、6.8%(6/88)、13.3%(13/98),放疗后(1例缺失)分别为1.1%(1/93)、12.4%(11/89)、12.2%(12/98),GEE模型多因素分析显示放疗未增加BCRL率(P=0.529)。线性逻辑回归模型多因素分析显示,与BMI低组相比中组和高组放疗前(RR=4.199,P=0.693和RR=10.999,P=0.002)、放疗后(RR=13.287,P=0.047和RR=14.308,P=0.029)的BCRL发生率差异具有统计学意义。按BMI<25、25~29、BMI≥30分组,分析结果与以上类似。结论:乳腺癌患者术后放疗发生BCRL,中国乳腺癌患者理想的BMI<28,与国外欧美患者为主的BMI<30不同。展开更多
High light induced photooxidation (HLIP) usually leads to leaf premature senescence and causes great yield loss in winter wheat. In order to explore the genetic control of wheat tolerance to HLIP stress, a quantitat...High light induced photooxidation (HLIP) usually leads to leaf premature senescence and causes great yield loss in winter wheat. In order to explore the genetic control of wheat tolerance to HLIP stress, a quantitative trait loci (QTL) analysis was conducted on a set of doubled haploid population, derived from two winter wheat cultivars. Actual values of chlorophyll content (Chl), minimum fluorescence level (Fo), maximum fluorescence level (Fm), and the maximum quan^m efficiency of photosystem II (Fv/Fm) under both HLIP and non-stress conditions as well as the ratios of HLIP to non-stress were evaluated. HLIP considerably reduced Chl, Fm, and Fv/Fm, but in- creased Fo, compared with that under non-stress condition. A total of 27, 16, and 28 QTLs were associated with the investigated traits under HLIP and non-stress and the ratios of HLIP to non-stress, respectively. Most of the QTLs for the ratios of HLIP to non-stress collocated or nearly linked with those detected under HLIP condition. HLIP-induced QTLs were mapped on 15 chromosomes, involving in 1A, 1B, 1D, 2A, 2B, 2D, 3A, 3B, 4A, 4D, 5B, 6A, 6B, 7A, and 7D while those expressed under non-stress condition involved in nine chromosomes, includ- ing 1B, 1D, 2A, 2B, 3B, 4A, 5A, 5B, and 7A. The expression patterns of QTLs under HLIP condition were different from that under non-stress condition except for six loci on five chromosomes. The phenotypic variance explained by individual QTL ranged from 5.0% to 19.7% under HLIP, 8.3% to 20.8% under non-stress, and 4.9% to 20.2% for the ratios of HLIP to non-stress, respectively. Some markers, for example, Xgwm192 and WMC331 on 4D regulating Chl, Fo, Fm, and Fv/Fm under HLIP condition, might be used in marker assistant selection.展开更多
Growth light intensities largely determine photo-synthesis, biomass, and grain yield of cereal crops. To explore the genetic basis of light responses of biomass and photosynthetic parameters in wheat (Triticum aestiv...Growth light intensities largely determine photo-synthesis, biomass, and grain yield of cereal crops. To explore the genetic basis of light responses of biomass and photosynthetic parameters in wheat (Triticum aestivum L.), a quantitative trait locus (QTL) analysis was carried out in a doubled haploid (DH) population grown in low light (LL), medium light (ML), and high light (HL), respectively. The results showed that the wheat seedlings grown in HL produced more biomass with lower total chlorophyll content (Chl), carotenoid content, and maximum photochemical efficiency of photosystem II (Fv/Fm) while the wheat seed-lings grown in LL produced less biomass with higher Chl compared with those grown in ML. In total, 48 QTLs were identified to be associated with the investigated parameters in relation to growth light intensities. These QTLs were mapped to 15 chromosomes which individually explained 6.3%-36.0% of the phenotypic variance, of which chromo-somes 3A, 1D, and 6B were specifically involved in LL response, 5D and 7A specifically involved in ML response, and 4B specifically involved in HL response. Several light-responsive QTLs were co-located with QTLs for photosyn-thetic parameters, biomass, and grain weight under various conditions which may provide new hints to uncover the genetic control of photosynthesis, biomass, and grain weight.展开更多
Ug99, also designated as TFKSK, is a race of Puccinia graminis Pers.:Pers f. sp. tn'tici Eriks. and E. Henn (Pgt) with broad virulence to wheat. It is the first known Pgt race possessing virulence to Sr31, a stem ...Ug99, also designated as TFKSK, is a race of Puccinia graminis Pers.:Pers f. sp. tn'tici Eriks. and E. Henn (Pgt) with broad virulence to wheat. It is the first known Pgt race possessing virulence to Sr31, a stem rust resistance (Sr) gene deployed in wheat varieties world- wide (Singh et al., 2011 ). Since the first detection of TFKSK in 1998, a total of 13 Ug99 variants have been identified in several African countries.展开更多
Stem rust, caused by Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn. (Pgt), is a destructive wheat disease and has the potential to cause significant yield losses (Olivera et al., 2015;Soko et al., ...Stem rust, caused by Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn. (Pgt), is a destructive wheat disease and has the potential to cause significant yield losses (Olivera et al., 2015;Soko et al., 2018). The emergence of Pgt race TTKSK, virulent to the widely deployed stem rust resistance gene Sr31 and originally named as isolate Pgt-Ug99 (Pretorius et al., 2000).展开更多
Stripe rust,caused by Puccinia striformis f.sp.tritici(Pst),is one of the most destructive diseases of wheat(Triticum aestivum L)worldwide.Xiaoyan 78829,a partial amphidiploid developed by crossing common wheat with T...Stripe rust,caused by Puccinia striformis f.sp.tritici(Pst),is one of the most destructive diseases of wheat(Triticum aestivum L)worldwide.Xiaoyan 78829,a partial amphidiploid developed by crossing common wheat with Thinopyrum intermedium,is immune to wheat stripe rust.To transfer the resis-tance gene of this excellent germplasm resource to wheat,the tr anslocation line WTT11 was produced by pollen irradiation and assessed for immunity to stripe rust races CYR32,CYR33 and CYR34.A novel stripe rust-resistance locus derived from Th.intermedium was confirmed by linkage and diagnostic marker analyses.Molecular cytogenetic analyses revealed that WTT11 carries a TTh 2DL translocation.The breakpoint of 1B was located at 95.5 MB,and the alien segments were found to be homoeologous to wheat-group chromosomes 6 and 7 according to a wheat660K single-nucleotide polymorphism(SNP)array analysis.Ten previously developed PCR-based markers were confirmed to rapidly trace the alien segments of WTT11,and 20 kompetitive allele-specific PCR(KASP)markers were developed to enable genotyping of Th.intermedium and common wheat.Evaluation of agronomic traits in two consecutive crop seasons uncovered some favorable agronomic traits in WTT11,such as lower plant height and longer main panicles,that may be applicable to wheat improvement.As a novel genetic resource,the new resistance locus may be useful for wheat disease-resistance breeding.展开更多
Dear Editor,Seed storage proteins (SSPs) are frequently important determinants of crop quality traits (Shewry and Casey, 1999). Dissecting and enhancing the genetic contributions of individual SSPs to their target...Dear Editor,Seed storage proteins (SSPs) are frequently important determinants of crop quality traits (Shewry and Casey, 1999). Dissecting and enhancing the genetic contributions of individual SSPs to their target traits are essential for effectively improving crop quality attributes. However, such a task is often difficult to accomplish, because SSPs are frequently expressed from multigene families and exhibit strong allelic variation. Consequently, detailed knowledge of the function of individual SSPs in crop quality trait is still limited. This scenario is well illustrated by high-molecular-weight glutenin subunits (HMW- GSs), a complex family of SSPs that are involved in wheat enduse quality through affecting dough functionality (Bek6s, 2012; Rasheed et al., 2014).展开更多
The translocation frequency up to 3.7% has been obtained by crossing a Triticum-Thinopyrum substitution with a Triticum-Hayna/dia substitution. The pattern of translocations includes not only wheat-alien Robertson tra...The translocation frequency up to 3.7% has been obtained by crossing a Triticum-Thinopyrum substitution with a Triticum-Hayna/dia substitution. The pattern of translocations includes not only wheat-alien Robertson translocations but also inserting translocations involving Thinopyrum chromatin and Haynaldia chromatin. The result indicates that it may be a new method of producing translocations to make two different wheat-alien substitution cross with each other.展开更多
Background Hypothalamic hamartoma(HH)is a congenital non-progressive lesion of hypothalamus during fetal development.Mass-like lesions in different anatomical locations often develop a variously disabling course prese...Background Hypothalamic hamartoma(HH)is a congenital non-progressive lesion of hypothalamus during fetal development.Mass-like lesions in different anatomical locations often develop a variously disabling course presenting with cognitive decline,psychiatric symptoms,as well as multiple seizure types.As a rare disease,HH is relatively common in infants and children,but it is extremely rare in adults.Case presentation We reported a case of adult-onset hypothalamic hamartoma,and summarized and analyzed relevant reports and studies of HH worldwide.The patient had clinical manifestations characterized by multiple seizure forms.After stereotactic radiofrequency thermocoagulation and drug treatment,the condition was effectively controlled.The patient was followed up till October 2022,with no recurrence of seizures.Conclusions Epilepsy caused by HH can resemble that of temporal lobe seizures,as HH forms a complex epileptogenic network with other regions of the brain through anatomical and functional connections.Early treatment of HH can provide better control of the symptoms of epilepsy,and patients with longer disease courses may have more complications.展开更多
Rates of photosynthesis, tolerance to photooxidative stress, and senescence are all important physiological factors that affect plant development and thus agricultural productivity. GRAS proteins play essential roles ...Rates of photosynthesis, tolerance to photooxidative stress, and senescence are all important physiological factors that affect plant development and thus agricultural productivity. GRAS proteins play essential roles in plant growth and development as well as in plant responses to biotic and abiotic stresses. So far few GRAS genes in wheat (Triticum aestivum L.) have been characterized. A previous transcriptome analysis indicated that the expression of a GRAS gene (TaSCL14) was induced by high-light stress in Xiaoyan 54 (XY54), a common wheat cultivar with strong tolerance to high-light stress. In this study, TaSCL14 gene was isolated from XY54 and mapped on chromosome 4A. TaSCL14 was expressed in various wheat organs, with high levels in stems and roots. Our results confirmed that TaSCL14 expression was indeed responsive to high-light stress. Barley stripe mosaic virus (BSMV)-based virus-induced gene silencing (VIGS) of TaSCL14 in wheat was performed to help characterize its potential functions. Silencing of TaSCL14 resulted in inhibited plant growth, decreased photosynthetic capacity, and reduced tolerance to photooxidative stress. In addition, silencing of TaSCL14 in wheat promoted leaf senescence induced by darkness. These results suggest that TaSCL14 may act as a multifunctional regulator involved in plant growth, photosynthesis, tolerance to photooxidative stress, and senescence.展开更多
Partial amphiploids created by crossing common wheat (Triticum aestivum L.) and Thinopyrum ponticum (Podp.) Barkworth & D. R. Dewey are important intermediates in wheat breeding because of their resistance to maj...Partial amphiploids created by crossing common wheat (Triticum aestivum L.) and Thinopyrum ponticum (Podp.) Barkworth & D. R. Dewey are important intermediates in wheat breeding because of their resistance to major wheat diseases. In this study, we examined the chromosome compositions of five Xiaoyan-series wheat-Th, ponticum partial amphiploids (Xiaoyan 68, Xiaoyan 693, Xiaoyan 784, Xiaoyan 7430, and Xiaoyan 7631) using GISH, multicolor-GISH, and multicolor-FISH. We found several chromosome changes in these lines. For example, wheat chromosomes 1B and 2B were added in Xiaoyan 68 and Xiaoyan 7430, respectively, while wheat chromosome 6B was eliminated from Xiaoyan 693 and Xiaoyan 7631. Chromosome rearrangements were also detected in these amphiploids, including an interspecific translocation involving chromosome 4D and some intergenomic translocations, such as A--B and A--D translocations, among wheat genomes. Analysis of the Th. ponticum chromosomes in the amphiploids showed that some lines shared the same alien chromosomes. We also evaluated these partial amphiploids for resistance to nine races of stem rust, including TTKSK (commonly known as Ug99). Three lines, Xiaoyan 68, Xiaoyan 784, and Xiaoyan 7430, exhibited excellent resistance to all nine races, and could therefore be valuable sources of stem rust resistance in wheat breeding.展开更多
TaPHT1.2 is a functional, root predominantly expressed and low phosphate (Pi) inducible high-affinity Pi transporter in wheat, which is more abundant in the roots of P-efficient wheat genotypes (e.g., Xiaoyan 54) ...TaPHT1.2 is a functional, root predominantly expressed and low phosphate (Pi) inducible high-affinity Pi transporter in wheat, which is more abundant in the roots of P-efficient wheat genotypes (e.g., Xiaoyan 54) than in P-inefficient genotypes (e.g., Jing 411) under both Pi-deficient and Pi-sufficient conditions. To characterize TaPHT1.2 further, we genetically mapped a TaPHT1.2 transporter, TaPHT1.2-D1, on the long arm of chromosome 4D using a recombinant inbred line population derived from Xiaoyan 54 and Jing 411, and isolated a 1,302 bp fragment of the TaPHT1.2-D1 promoter (PrTaPHT1.2-D1) from Xiaoyan 54. TaPHT1.2-D1 shows collinearity with OsPHT1.2 that has previously been reported to mediate the translocation of Pi from roots to shoots. PrTaPHT1.2-D contains a number of Pi-starvation responsive elements, including P1BS, WRKY-binding W-box, and helix-loop-helix-binding elements. PrTaPHT1.2-D1 was then used to drive expression of 13-glucuronidase (GUS) reporter gene in Arabidopsis through Agrobacterium-mediated transformation. Histochemical analysis of transgenic Arabidopsis plants showed that the reporter gene was specifically induced by Pi-starvation and predominantly expressed in the roots. As there is only one SNP between the TaPHT1.2-D1 promoters of Xiaoyan 54 and Jing 411, and this SNP does not exist within the Pi-starvation responsive elements, the differential expression of TaPHT1.2 in Xiaoyan 54 and Jing 411 may not be caused by this SNP.展开更多
In order to explore the genetics of dark-induced senescence in winter wheat (Triticum aestivum L.), a quantitative trait loci (QTL) analysis was carried out in a doubled haploid population developed from a cross b...In order to explore the genetics of dark-induced senescence in winter wheat (Triticum aestivum L.), a quantitative trait loci (QTL) analysis was carried out in a doubled haploid population developed from a cross between the varieties Hanxuan 10 (HX) and Lumai 14 (LM). The senescence parameters chlorophyll content (Chl a+b, Chl a, and Chl b), original fluorescence (Fo), maximum fluorescence level (Fm), maximum photochemical efficiency (FvlFm), and ratio of variable fluorescence to original fluorescence (FvlFo) were evaluated in the second leaf of whole three-leaf seedlings subjected to 7 d of darkness. A total of 43 QTLs were identified that were associated with dark-induced senescence using composite interval mapping. These QTLs were mapped to 20 loci distributed on 11 chromosomes: 1B, 1D, 2A, 2B, 3B, 3D, 5D, 6A, 6B, 7A, and 7B. The phenotypic variation explained by each QTL ranged from 7.5% to 19.4%. Eleven loci coincided with two or more of the analyzed parameters. In addition, 14 loci co-located or were linked with previously reported QTLs regulating flag leaf senescence, tolerance to high light stress, and grain protein content (Gpc), separately.展开更多
The wild relatives of wheat (Triticum aestivum L.) contain tremendous amounts of potentially useful genes and represent a promising source of genetic diversity for wheat improvement (Bommineni and Jauhar, 1997). T...The wild relatives of wheat (Triticum aestivum L.) contain tremendous amounts of potentially useful genes and represent a promising source of genetic diversity for wheat improvement (Bommineni and Jauhar, 1997). Thinopyrum ponticum (Popd.) Barkworth and D. R. Dewey [syn. Agropyron elongatum (Host) P. Beauv., Elytrigia pontica (Podp.) Holub, Lophopy- rum ponticum (Podp.) A. L6ve] (2n = 10x = 70), has high crossability with various Triticum species. Numerous studies have shown that Th. ponticum carries many potentially valu- able resistance genes against biotic and abiotic stresses (Shannon, 1978; Cox, 1991; Zheng et al., 2014a,b). Transferring the useful genes from Th. ponticum to common wheat through chromosome engineering had been a successful way to enhance the resistance of wheat to pests and diseases (Sharma et al., 1989; McIntosh, 1991).展开更多
基金supported by the Guangdong Province Medical Science Research Fund, No. B200258
文摘Major ozonated autohemotherapy is classically used in treating ischemic disorder of the lower limbs In the present study, we performed major ozonated autohemotherapy treatment in patients with acute cerebral infarction, and assessed outcomes according to the U.S. National Institutes of Health Stroke Score, Modified Rankin Scale, and transcranial magnetic stimulation motor-evoked potential. Compared with the control group, the clinical total effective rate and the cortical potential rise rate of the upper limbs were significantly higher, the central motor conduction time of upper limb was significantly shorter, and the upper limb motor-evoked potential amplitude was significantly increased, in the ozone group. In the ozone group, the National Institutes of Health Stroke Score was positively correlated with the central motor conduction time and the motor-evoked potential amplitude of the upper limb. Central motor conduction time and motor-evoked potential amplitude of the upper limb may be effective indicators of motor-evoked potentials to assess upper limb motor function in cerebral infarct patients. Furthermore, major ozonated autohemotherapy may promote motor function recovery of the upper limb in patients with acute cerebral infarction.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA08030105)Key Programs of the Chinese Academy of Sciences (KFZD-SW112)STS Project of Chinese Academy of Sciences (KFJ-STS-ZDTP-024)
文摘Xiaoyan 60(XY60) is a new wheat variety bred in the laboratory of Zhensheng Li.After salt treatment, seedlings of XY60 maintain green leaves and produce longer roots than the high yielding cultivar Zhongmai 175(ZM175).To explain these different phenotypes we carried out an RNA-Seq analysis using 12 samples from three tissues of both varieties subjected to salt and control treatments.By comparing data from the salt treated plants with the control, 703, 979, and 1197 differentially expressed genes(DEGs) were detected in new leaves, old leaves, and roots of XY60, respectively.The corresponding numbers for ZM175 were 613, 1401, and 1301.The most significantly enriched Gene Ontology(GO) terms and KEGG pathways were associated with polyunsaturated fatty acid(PUFA) metabolism in both new and old leaves from XY60.They were associated with photosynthesis and energy metabolism in ZM175.The most significantly enriched KEGG pathway in roots of both varieties was “glucosinolate biosynthesis”.In addition, jasmonic acid(JA) concentration in XY60 was higher than in ZM175, although it increased significantly in both varieties following salt treatment.Trends in relative expression levels of AOS, MYC2, and JAZ revealed by qRT-PCR were concordant with those from RNA-Seq.Our results suggest that PUFAs may contribute to salt tolerance in common wheat by enhancing the photosynthetic system and JA-related pathways.
文摘目的:探讨身体质量指数(body mass index,BMI)与放疗前、后乳腺癌相关淋巴水肿(breast cancer related lymphedema,BCRL)的相关性。方法:分析2013年11月至2015年2月281例于河北医科大学第四医院收治的单侧乳腺癌术后女性患者的前瞻性临床资料,根据BMI<25、25~27、BMI≥28分为BMI低组(n=94)、中组(n=89)、高组(n=98),比较健患侧上肢体积差(upper limb volume difference,ULVD),采用广义估计方程(GEE)模型和线性逻辑回归模型进行单因素和多因素分析,评估放疗对BCRL(定义为ULVD≥200 m L)的影响,并与其他BMI分界值分组结果进行比较。结果:放疗前、后ULVD平均值分别为40.6、42.9 m L,中位值均为30.0 m L,二者之间差异无统计学意义(P>0.05)。放疗前(2例缺失)BMI低、中、高组的BCRL发生率分别为2.2%(2/93)、6.8%(6/88)、13.3%(13/98),放疗后(1例缺失)分别为1.1%(1/93)、12.4%(11/89)、12.2%(12/98),GEE模型多因素分析显示放疗未增加BCRL率(P=0.529)。线性逻辑回归模型多因素分析显示,与BMI低组相比中组和高组放疗前(RR=4.199,P=0.693和RR=10.999,P=0.002)、放疗后(RR=13.287,P=0.047和RR=14.308,P=0.029)的BCRL发生率差异具有统计学意义。按BMI<25、25~29、BMI≥30分组,分析结果与以上类似。结论:乳腺癌患者术后放疗发生BCRL,中国乳腺癌患者理想的BMI<28,与国外欧美患者为主的BMI<30不同。
基金supported by the National Key Basic Research Program (Nos. 2009CB118506, 2004CB117205, and 2002CB111304)the Natural Science Foundation of China (No. 30330390)+1 种基金the Knowledge Innovation Program (KIP) key project from the Chinese Academy of Sciences (No. KSCX1-YW-03)the National Science and Tech-nology Supporting Program (No. 2006GB24910466)
文摘High light induced photooxidation (HLIP) usually leads to leaf premature senescence and causes great yield loss in winter wheat. In order to explore the genetic control of wheat tolerance to HLIP stress, a quantitative trait loci (QTL) analysis was conducted on a set of doubled haploid population, derived from two winter wheat cultivars. Actual values of chlorophyll content (Chl), minimum fluorescence level (Fo), maximum fluorescence level (Fm), and the maximum quan^m efficiency of photosystem II (Fv/Fm) under both HLIP and non-stress conditions as well as the ratios of HLIP to non-stress were evaluated. HLIP considerably reduced Chl, Fm, and Fv/Fm, but in- creased Fo, compared with that under non-stress condition. A total of 27, 16, and 28 QTLs were associated with the investigated traits under HLIP and non-stress and the ratios of HLIP to non-stress, respectively. Most of the QTLs for the ratios of HLIP to non-stress collocated or nearly linked with those detected under HLIP condition. HLIP-induced QTLs were mapped on 15 chromosomes, involving in 1A, 1B, 1D, 2A, 2B, 2D, 3A, 3B, 4A, 4D, 5B, 6A, 6B, 7A, and 7D while those expressed under non-stress condition involved in nine chromosomes, includ- ing 1B, 1D, 2A, 2B, 3B, 4A, 5A, 5B, and 7A. The expression patterns of QTLs under HLIP condition were different from that under non-stress condition except for six loci on five chromosomes. The phenotypic variance explained by individual QTL ranged from 5.0% to 19.7% under HLIP, 8.3% to 20.8% under non-stress, and 4.9% to 20.2% for the ratios of HLIP to non-stress, respectively. Some markers, for example, Xgwm192 and WMC331 on 4D regulating Chl, Fo, Fm, and Fv/Fm under HLIP condition, might be used in marker assistant selection.
基金supported by the National Key Basic Research Program (2009CB118506)the Knowledge Innovation Program (KIP) key project from the Chinese Academy of Sciences (KSCX2-EW-N-02)the Natural Science Foundation of China (31371609)
文摘Growth light intensities largely determine photo-synthesis, biomass, and grain yield of cereal crops. To explore the genetic basis of light responses of biomass and photosynthetic parameters in wheat (Triticum aestivum L.), a quantitative trait locus (QTL) analysis was carried out in a doubled haploid (DH) population grown in low light (LL), medium light (ML), and high light (HL), respectively. The results showed that the wheat seedlings grown in HL produced more biomass with lower total chlorophyll content (Chl), carotenoid content, and maximum photochemical efficiency of photosystem II (Fv/Fm) while the wheat seed-lings grown in LL produced less biomass with higher Chl compared with those grown in ML. In total, 48 QTLs were identified to be associated with the investigated parameters in relation to growth light intensities. These QTLs were mapped to 15 chromosomes which individually explained 6.3%-36.0% of the phenotypic variance, of which chromo-somes 3A, 1D, and 6B were specifically involved in LL response, 5D and 7A specifically involved in ML response, and 4B specifically involved in HL response. Several light-responsive QTLs were co-located with QTLs for photosyn-thetic parameters, biomass, and grain weight under various conditions which may provide new hints to uncover the genetic control of photosynthesis, biomass, and grain weight.
基金supported by the Ministry of Science and Technology of China (No. 2014DFA31540)Chinese Academy of Sciences (No. SAJC201305)the Bill & Melinda Gates Foundation to Cornell University for the Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project
文摘Ug99, also designated as TFKSK, is a race of Puccinia graminis Pers.:Pers f. sp. tn'tici Eriks. and E. Henn (Pgt) with broad virulence to wheat. It is the first known Pgt race possessing virulence to Sr31, a stem rust resistance (Sr) gene deployed in wheat varieties world- wide (Singh et al., 2011 ). Since the first detection of TFKSK in 1998, a total of 13 Ug99 variants have been identified in several African countries.
基金supported by the Natural Science Foundation of China (No. 31571644)the National Key Basic Research Program (No. 2015CB150106)the Ministry of Science and Technology of China (2014DFA31540)
文摘Stem rust, caused by Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn. (Pgt), is a destructive wheat disease and has the potential to cause significant yield losses (Olivera et al., 2015;Soko et al., 2018). The emergence of Pgt race TTKSK, virulent to the widely deployed stem rust resistance gene Sr31 and originally named as isolate Pgt-Ug99 (Pretorius et al., 2000).
基金the National Key Research and Development Pro-gram of China(2016YFD0102000)the National Natural Sci-ence Foundation of China(no.31971875).
文摘Stripe rust,caused by Puccinia striformis f.sp.tritici(Pst),is one of the most destructive diseases of wheat(Triticum aestivum L)worldwide.Xiaoyan 78829,a partial amphidiploid developed by crossing common wheat with Thinopyrum intermedium,is immune to wheat stripe rust.To transfer the resis-tance gene of this excellent germplasm resource to wheat,the tr anslocation line WTT11 was produced by pollen irradiation and assessed for immunity to stripe rust races CYR32,CYR33 and CYR34.A novel stripe rust-resistance locus derived from Th.intermedium was confirmed by linkage and diagnostic marker analyses.Molecular cytogenetic analyses revealed that WTT11 carries a TTh 2DL translocation.The breakpoint of 1B was located at 95.5 MB,and the alien segments were found to be homoeologous to wheat-group chromosomes 6 and 7 according to a wheat660K single-nucleotide polymorphism(SNP)array analysis.Ten previously developed PCR-based markers were confirmed to rapidly trace the alien segments of WTT11,and 20 kompetitive allele-specific PCR(KASP)markers were developed to enable genotyping of Th.intermedium and common wheat.Evaluation of agronomic traits in two consecutive crop seasons uncovered some favorable agronomic traits in WTT11,such as lower plant height and longer main panicles,that may be applicable to wheat improvement.As a novel genetic resource,the new resistance locus may be useful for wheat disease-resistance breeding.
文摘Dear Editor,Seed storage proteins (SSPs) are frequently important determinants of crop quality traits (Shewry and Casey, 1999). Dissecting and enhancing the genetic contributions of individual SSPs to their target traits are essential for effectively improving crop quality attributes. However, such a task is often difficult to accomplish, because SSPs are frequently expressed from multigene families and exhibit strong allelic variation. Consequently, detailed knowledge of the function of individual SSPs in crop quality trait is still limited. This scenario is well illustrated by high-molecular-weight glutenin subunits (HMW- GSs), a complex family of SSPs that are involved in wheat enduse quality through affecting dough functionality (Bek6s, 2012; Rasheed et al., 2014).
文摘The translocation frequency up to 3.7% has been obtained by crossing a Triticum-Thinopyrum substitution with a Triticum-Hayna/dia substitution. The pattern of translocations includes not only wheat-alien Robertson translocations but also inserting translocations involving Thinopyrum chromatin and Haynaldia chromatin. The result indicates that it may be a new method of producing translocations to make two different wheat-alien substitution cross with each other.
基金supported by the foundation of Medical Science and Technology of Guangdong(A2022402).
文摘Background Hypothalamic hamartoma(HH)is a congenital non-progressive lesion of hypothalamus during fetal development.Mass-like lesions in different anatomical locations often develop a variously disabling course presenting with cognitive decline,psychiatric symptoms,as well as multiple seizure types.As a rare disease,HH is relatively common in infants and children,but it is extremely rare in adults.Case presentation We reported a case of adult-onset hypothalamic hamartoma,and summarized and analyzed relevant reports and studies of HH worldwide.The patient had clinical manifestations characterized by multiple seizure forms.After stereotactic radiofrequency thermocoagulation and drug treatment,the condition was effectively controlled.The patient was followed up till October 2022,with no recurrence of seizures.Conclusions Epilepsy caused by HH can resemble that of temporal lobe seizures,as HH forms a complex epileptogenic network with other regions of the brain through anatomical and functional connections.Early treatment of HH can provide better control of the symptoms of epilepsy,and patients with longer disease courses may have more complications.
基金financially supported by the National Natural Science Foundation of China(No.31371609)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA08010403)the National Key Basic Research Program(No.2009CB118506)
文摘Rates of photosynthesis, tolerance to photooxidative stress, and senescence are all important physiological factors that affect plant development and thus agricultural productivity. GRAS proteins play essential roles in plant growth and development as well as in plant responses to biotic and abiotic stresses. So far few GRAS genes in wheat (Triticum aestivum L.) have been characterized. A previous transcriptome analysis indicated that the expression of a GRAS gene (TaSCL14) was induced by high-light stress in Xiaoyan 54 (XY54), a common wheat cultivar with strong tolerance to high-light stress. In this study, TaSCL14 gene was isolated from XY54 and mapped on chromosome 4A. TaSCL14 was expressed in various wheat organs, with high levels in stems and roots. Our results confirmed that TaSCL14 expression was indeed responsive to high-light stress. Barley stripe mosaic virus (BSMV)-based virus-induced gene silencing (VIGS) of TaSCL14 in wheat was performed to help characterize its potential functions. Silencing of TaSCL14 resulted in inhibited plant growth, decreased photosynthetic capacity, and reduced tolerance to photooxidative stress. In addition, silencing of TaSCL14 in wheat promoted leaf senescence induced by darkness. These results suggest that TaSCL14 may act as a multifunctional regulator involved in plant growth, photosynthesis, tolerance to photooxidative stress, and senescence.
基金supported by the grants from the National Natural Science Foundation of China(No.31171539)the National High-Tech Research and Development Program of China(No.2011AA1001)the National Key Technology R&D Program of China(No.2013BAD05B01)
文摘Partial amphiploids created by crossing common wheat (Triticum aestivum L.) and Thinopyrum ponticum (Podp.) Barkworth & D. R. Dewey are important intermediates in wheat breeding because of their resistance to major wheat diseases. In this study, we examined the chromosome compositions of five Xiaoyan-series wheat-Th, ponticum partial amphiploids (Xiaoyan 68, Xiaoyan 693, Xiaoyan 784, Xiaoyan 7430, and Xiaoyan 7631) using GISH, multicolor-GISH, and multicolor-FISH. We found several chromosome changes in these lines. For example, wheat chromosomes 1B and 2B were added in Xiaoyan 68 and Xiaoyan 7430, respectively, while wheat chromosome 6B was eliminated from Xiaoyan 693 and Xiaoyan 7631. Chromosome rearrangements were also detected in these amphiploids, including an interspecific translocation involving chromosome 4D and some intergenomic translocations, such as A--B and A--D translocations, among wheat genomes. Analysis of the Th. ponticum chromosomes in the amphiploids showed that some lines shared the same alien chromosomes. We also evaluated these partial amphiploids for resistance to nine races of stem rust, including TTKSK (commonly known as Ug99). Three lines, Xiaoyan 68, Xiaoyan 784, and Xiaoyan 7430, exhibited excellent resistance to all nine races, and could therefore be valuable sources of stem rust resistance in wheat breeding.
基金supported by the Ministry of Science and Technology of China (No.2005CB120904 and 2006AA10A105)the National Natural Science Foundation of China (No.30890133 and 30521001)the Chinese Academy of Sciences (No.KSCX2-YW-N-001)
文摘TaPHT1.2 is a functional, root predominantly expressed and low phosphate (Pi) inducible high-affinity Pi transporter in wheat, which is more abundant in the roots of P-efficient wheat genotypes (e.g., Xiaoyan 54) than in P-inefficient genotypes (e.g., Jing 411) under both Pi-deficient and Pi-sufficient conditions. To characterize TaPHT1.2 further, we genetically mapped a TaPHT1.2 transporter, TaPHT1.2-D1, on the long arm of chromosome 4D using a recombinant inbred line population derived from Xiaoyan 54 and Jing 411, and isolated a 1,302 bp fragment of the TaPHT1.2-D1 promoter (PrTaPHT1.2-D1) from Xiaoyan 54. TaPHT1.2-D1 shows collinearity with OsPHT1.2 that has previously been reported to mediate the translocation of Pi from roots to shoots. PrTaPHT1.2-D contains a number of Pi-starvation responsive elements, including P1BS, WRKY-binding W-box, and helix-loop-helix-binding elements. PrTaPHT1.2-D1 was then used to drive expression of 13-glucuronidase (GUS) reporter gene in Arabidopsis through Agrobacterium-mediated transformation. Histochemical analysis of transgenic Arabidopsis plants showed that the reporter gene was specifically induced by Pi-starvation and predominantly expressed in the roots. As there is only one SNP between the TaPHT1.2-D1 promoters of Xiaoyan 54 and Jing 411, and this SNP does not exist within the Pi-starvation responsive elements, the differential expression of TaPHT1.2 in Xiaoyan 54 and Jing 411 may not be caused by this SNP.
基金supported by the National Basic Research Program of China (2009CB118506 and 2009CB118300)the National Natural Science Foundation of China (30800683)the Knowledge Innovation Program Key Project from the Chinese Academy of Sciences (KSCX1-YW-03 and KSCX2-EW-N-02)
文摘In order to explore the genetics of dark-induced senescence in winter wheat (Triticum aestivum L.), a quantitative trait loci (QTL) analysis was carried out in a doubled haploid population developed from a cross between the varieties Hanxuan 10 (HX) and Lumai 14 (LM). The senescence parameters chlorophyll content (Chl a+b, Chl a, and Chl b), original fluorescence (Fo), maximum fluorescence level (Fm), maximum photochemical efficiency (FvlFm), and ratio of variable fluorescence to original fluorescence (FvlFo) were evaluated in the second leaf of whole three-leaf seedlings subjected to 7 d of darkness. A total of 43 QTLs were identified that were associated with dark-induced senescence using composite interval mapping. These QTLs were mapped to 20 loci distributed on 11 chromosomes: 1B, 1D, 2A, 2B, 3B, 3D, 5D, 6A, 6B, 7A, and 7B. The phenotypic variation explained by each QTL ranged from 7.5% to 19.4%. Eleven loci coincided with two or more of the analyzed parameters. In addition, 14 loci co-located or were linked with previously reported QTLs regulating flag leaf senescence, tolerance to high light stress, and grain protein content (Gpc), separately.
基金supported by the grants from the National High-Tech Research and Development Program of China (No. 2011AA1001)the National Key Technology R&D Program of China (No. 2013BAD05B01)the National Natural Science Foundation of China (No. 31171539)
文摘The wild relatives of wheat (Triticum aestivum L.) contain tremendous amounts of potentially useful genes and represent a promising source of genetic diversity for wheat improvement (Bommineni and Jauhar, 1997). Thinopyrum ponticum (Popd.) Barkworth and D. R. Dewey [syn. Agropyron elongatum (Host) P. Beauv., Elytrigia pontica (Podp.) Holub, Lophopy- rum ponticum (Podp.) A. L6ve] (2n = 10x = 70), has high crossability with various Triticum species. Numerous studies have shown that Th. ponticum carries many potentially valu- able resistance genes against biotic and abiotic stresses (Shannon, 1978; Cox, 1991; Zheng et al., 2014a,b). Transferring the useful genes from Th. ponticum to common wheat through chromosome engineering had been a successful way to enhance the resistance of wheat to pests and diseases (Sharma et al., 1989; McIntosh, 1991).