Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological status.Although a broad spectrum...Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological status.Although a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly characterized.An immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this need.Here,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory factors.To fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant.展开更多
The therapeutic efficacy of radioimmunotherapy against triple negative breast cancer(TNBC)is largely limited by the complicated tumor microenvironment(TME)and its immunosuppressive state.Thus developing a strategy to ...The therapeutic efficacy of radioimmunotherapy against triple negative breast cancer(TNBC)is largely limited by the complicated tumor microenvironment(TME)and its immunosuppressive state.Thus developing a strategy to reshape TME is expected to achieve highly efficient radioimmunotherapy.Therefore,we designed and synthesized a tellurium(Te)-driven maple leaf manganese carbonate nanotherapeutics(MnCO3@Te)by gas diffusion method,but also provided a chemical catalytic strategy in situ to augment ROS level and activate immune cells for improving cancer radioimmunotherapy.As expected,with the help of H2O2 in TEM,MnCO3@Te heterostructure with reversible Mn3+/Mn2+transition could catalyze the intracellular ROS overproduction to amplify radiotherapy.In addition,by virtue of the ability to scavenge H+in TME by carbonate group,MnCO3@Te directly promote the maturation of dendritic cells and macrophage M1 repolarization by stimulator of interferon genes(STING)pathway activation,resulting in remodeling immuno-microenvironment.As a result,MnCO3@Te synergized with radiotherapy and immune checkpoint blockade therapy effectively inhibited the breast cancer growth and lung metastasis in vivo.Collectively,these findings indicate that MnCO3@Te as an agonist,successfully overcome radioresistance and awaken immune systems,showing promising potential for solid tumor radioimmunotherapy.展开更多
Aging increases the risk of various diseases. The main goal of aging research is to find therapies that attenuate aging and alleviate aging-related diseases. In this study, we screened a natural product library for ge...Aging increases the risk of various diseases. The main goal of aging research is to find therapies that attenuate aging and alleviate aging-related diseases. In this study, we screened a natural product library for geroprotective compounds using Werner syndrome (WS) human mesenchymal stem cells (hMSCs), a premature aging model that we recently established. Ten candidate compounds were identified and quercetin was investigated in detail due to its leading effects. Mechanistic studies revealed that quercetin alleviated senescence via the enhancement of cell proliferation and restoration of heterochromatin architecture in WS hMSCs. RNA-sequencing analysis revealed the transcriptional commonalities and differences in the geroprotective effects by quercetin and Vitamin C. Besides WS hMSCs, quercetin also attenuated cellular senescence in Hutchinson-Gilford progeria syndrome (HGPS) and physiological-aging hMSCs. Taken together, our study identifies quercetin as a geroprotective agent against accelerated and natural aging in hMSCs, providing a potential therapeutic intervention for treating age-associated disorders.展开更多
Vascular cell functionality is critical to blood vessel homeostasis. Constitutive NF-κB activation in vascular cells results in chronic vascular inflammation, leading to various cardiovascular diseases. However, how ...Vascular cell functionality is critical to blood vessel homeostasis. Constitutive NF-κB activation in vascular cells results in chronic vascular inflammation, leading to various cardiovascular diseases. However, how NF-κB regulates human blood vessel homeostasis remains largely elusive. Here, using CRISPR/Cas9-mediated gene editing, we generated RelA knockout human embryonic stem cells (hESCs) and differentiated them into various vascular cell derivatives to study how NF- KS modulates human vascular cells under basal and inflammatory conditions. Multi-dimensional phenotypic assessments and transcriptomic analyses revealed that RelA deficiency affected vascular cells via modulatinginflammation, survival, vasculogenesis, cell differentia- tion and extracellular matrix organization in a cell type- specific manner under basal condition, and that RelA protected vascular cells against apoptosis and modu- lated vascular inflammatory response upon tumor necrosis factor a (TNFa) stimulation. Lastly, further evaluation of gene expression patterns in IKBo knockout vascular cells demonstrated that IKBa acted largely independent of RelA signaling. Taken together, our data reveal a protective role of NF-κB/ReiA in modulating human blood vessel homeostasis and map the human vascular transcriptomic landscapes for the discovery of novel therapeutic targets.展开更多
Remarkable progress in ageing research has been achieved over the past decades.General perceptions and experimental evidence pinpoint that the decline of physical function often initiates by cell senescence and organ ...Remarkable progress in ageing research has been achieved over the past decades.General perceptions and experimental evidence pinpoint that the decline of physical function often initiates by cell senescence and organ ageing.Epigenetic dynamics and immunometabolic reprogramming link to the alterations of cellular response to intrinsic and extrinsic stimuli,representing current hotspots as they not only(re-)shape the individual cell identity,but also involve in cell fate decision.This review focuses on the present findings and emerging concepts in epigenetic,inflammatory,and metabolic regulations and the consequences of the ageing process.Potential therapeutic interventions targeting cell senescence and regulatory mechanisms,using state-of-the-art techniques are also discussed.展开更多
Background:Epidermal stem cells(EpSCs)that reside in cutaneous hair follicles and the basal layer of the epidermis are indispensable for wound healing and skin homeostasis.Little is known about the effects of photoche...Background:Epidermal stem cells(EpSCs)that reside in cutaneous hair follicles and the basal layer of the epidermis are indispensable for wound healing and skin homeostasis.Little is known about the effects of photochemical activation on EpSC differentiation,proliferation and migration during wound healing.The present study aimed to determine the effects of photodynamic therapy(PDT)on wound healing in vivo and in vitro.Methods:We created mouse full-thickness skin resection models and applied 5-aminolevulinic acid(ALA)for PDT to the wound beds.Wound healing was analysed by gross evaluation and haematoxylin–eosin staining in vivo.In cultured EpSCs,protein expression was measured using flow cytometry and immunohistochemistry.Cell migration was examined using a scratch model;apoptosis and differentiation were measured using flow cytometry.Results:PDT accelerated wound closure by enhancing EpSC differentiation,proliferation and migration,thereby promoting re-epithelialization and angiogenesis.PDT inhibited inflammatory infiltration and expression of proinflammatory cytokines,whereas the secretion of growth factors was greater than in other groups.The proportion of transient amplifying cells was significantly greater in vivo and in vitro in the PDT groups.EpSC migration was markedly enhanced after ALAinduced PDT.Conclusions:Topical ALA-induced PDT stimulates wound healing by enhancing re-epithelialization,promoting angiogenesis as well as modulating skin homeostasis.This work provides a preliminary theoretical foundation for the clinical administration of topical ALA-induced PDT in skin wound healing.展开更多
Eosinophils are terminally differentiated cells derived from hematopoietic stem cells(HSCs)in the bone marrow.Several studies have confirmed the effective roles of eosinophils in asthmatic airway pathogenesis.However,...Eosinophils are terminally differentiated cells derived from hematopoietic stem cells(HSCs)in the bone marrow.Several studies have confirmed the effective roles of eosinophils in asthmatic airway pathogenesis.However,their regulatory functions have not been well elucidated.Here,increased C-C chemokine ligand 6(CCL6)in asthmatic mice and the human orthologs CCL15 and CCL23 that are highly expressed in asthma patients are described,which are mainly derived from eosinophils.Using Cc/6 knockout mice,further studies revealed CCL6-dependent allergic airway inflammation and committed eosinophilia in the bone marrow following ovalbumin(OVA)challenge and identified a CCL6-CCR1 regulatory axis in hematopoietic stem cells(HSCs).Eosinophil differentiation and airway inflammation were remarkably decreased by the specific CCR1 antagonist BX471.Thus,the study identifies that the CCL6-CCR1 axis is involved in the crosstalk between eosinophils and HSCs during the development of allergic airway inflammation,which also reveals a potential therapeutic strategy for targeting G protein-coupled receptors(GPCRs)for future clinical treatment of asthma.展开更多
Susceptibility of gastrointestinal dysmotility increases with age-associated colonic degeneration.A paucity of remedies reversing colonic degeneration per se hinders the fundamental relief of symptoms.Here we discover...Susceptibility of gastrointestinal dysmotility increases with age-associated colonic degeneration.A paucity of remedies reversing colonic degeneration per se hinders the fundamental relief of symptoms.Here we discovered the correlation between colon degeneration and altered nicotinamide adenine dinucleotide(NAD)level in aged mice.Compared to 3-month-old young controls,2-year-old mice showed a spectrum of degenerative colonic phenotypes and exhibited a significant elongated transit time and slowed stool frequency in the context of Lomotil-induced slow-transit constipation.Despite upregulated colonic tryptophan hydroxylases expression,serotonin release and expression of colon-predominant type IV serotonin receptor,reduced viability of interstitial cells of Cajal while enhanced aquaporins(Aqp1,3 and 11)led to a less colonic motility and increased luminal dehydration in aged mice.Notably,this colonic degeneration was accompanied with reduced key NAD^(+)-generating enzyme expression and lowered NAD^(+)/NADH ratio in aged colon.Three-month continuous administration of beta nicotinamide mononucleotide,a NAD^(+)precursor,elevated colonic NAD^(+)level and improved defecation in aged mice.In contrast,pharmacological inhibition of nicotinamide phosphoribosyltransferase,the rate-limiting enzyme for NAD^(+)biosynthesis,induced a reduction in colonic NAD content and impaired gastrointestinal function in young mice.Taken together,these findings suggest the beneficial effect of NAD^(+)in maintaining colonic homoeostasis and reactivating NAD^(+)biosynthesis may represent a promising strategy to counteract age-related gastrointestinal degeneration.展开更多
Embryonic stem cells (ESCs) are a potential source of generating transplantable hematopoietic stem and progenitor cells,which in turn can serve as "seed" cells for hematopoietic regeneration.In this study,we aimed...Embryonic stem cells (ESCs) are a potential source of generating transplantable hematopoietic stem and progenitor cells,which in turn can serve as "seed" cells for hematopoietic regeneration.In this study,we aimed to gauge the ability of mouse ESCs directly differentiating into hematopoietic cells in adult bone marrow (BM).To this end,we first derived a new mouse ESC line that constitutively expressed the green fluorescent protein (GFP) and then injected the ESCs into syngeneic BM via intra-tibia.The progeny of the transplanted ESCs were then analyzed at different time points after transplantation.Notably,however,most injected ESCs differentiated into non-hematopoietic cells in the BM whereas only a minority of the cells acquired hematopoietic cell surface markers.This study provides a strategy for evaluating the differentiation potential of ESCs in the BM micro-environment,thereby having important implications for the physiological maintenance and potential therapeutic applications of ESCs.展开更多
The multimode fiber(MMF)has great potential to transmit high-resolution images with less invasive methods in endoscopy due to its large number of spatial modes and small core diameter.However,spatial modes crosstalk w...The multimode fiber(MMF)has great potential to transmit high-resolution images with less invasive methods in endoscopy due to its large number of spatial modes and small core diameter.However,spatial modes crosstalk will inevitably occur in MMFs,which makes the received images become speckles.A conditional generative adversarial network(GAN)composed of a generator and a discriminator was utilized to reconstruct the received speckles.We conduct an MMF imaging experimental system of transmitting over 1 m MMF with a 50μm core.Compared with the conventional method of U-net,this conditional GAN could reconstruct images with fewer training datasets to achieve the same performance and shows higher feature extraction capability.展开更多
Dear Editor,Emerging evidences show close associations between miRNAs and liver regeneration.MiR-126 is known as a governor of vascular integrity and angiogenesis.MiR-126 is involved in the self-renewal of hematopoiet...Dear Editor,Emerging evidences show close associations between miRNAs and liver regeneration.MiR-126 is known as a governor of vascular integrity and angiogenesis.MiR-126 is involved in the self-renewal of hematopoietic stem cells(HSCS)and leukemia stem cells.1,2 MiR-126 also plays a vital role in hepatocellular carcinogenesis,however,the functions of miR-126 in liver regeneration were still unclear.展开更多
Maintenance of tissue-specific stem cells is vital for organ homeostasis and organismal longevity.Hematopoietic stem cells(HSCs) are the most primitive cell type in the hematopoietic system.They divide asymmetricall...Maintenance of tissue-specific stem cells is vital for organ homeostasis and organismal longevity.Hematopoietic stem cells(HSCs) are the most primitive cell type in the hematopoietic system.They divide asymmetrically and give rise to daughter cells with HSC identity(selfrenewal) and progenitor progenies(differentiation),which further proliferate and differentiate into full hematopoietic lineages.Mammalian ageing process is accompanied with abnormalities in the HSC self-renewal and differentiation.Transcriptional changes and epigenetic modulations have been implicated as the key regulators in HSC ageing process.The DNA damage response(DDR)in the cells involves an orchestrated signaling pathway,consisting of cell cycle regulation,cell death and senescence,transcriptional regulation,as well as chromatin remodeling.Recent studies employing DNA repair-deficient mouse models indicate that DDR could intrinsically and extrinsically regulate HSC maintenance and play important roles in tissue homeostasis of the hematopoietic system.In this review,we summarize the current understanding of how the DDR determines the HSC fates and finally contributes to organismal ageing.展开更多
The biogenesis of autophagosomes provides the basis for macroautophagy to capture and degrade intracellular cargoes.Binding of the autophagy-related protein ATG8/LC3 to autophagic membranes is essential to autophagoso...The biogenesis of autophagosomes provides the basis for macroautophagy to capture and degrade intracellular cargoes.Binding of the autophagy-related protein ATG8/LC3 to autophagic membranes is essential to autophagosome formation,which involves the specific and dynamic processing of ATG8/LC3 by cysteine protease ATG4.However,to date,the mechanism whereby ATG4 is recruited to the membranes,the interaction of ATG4 and ATG8/LC3 on the membranes,and its role in the growth of phagophore are not completely understood.Here,we used fluorescence recovery after photobleaching to monitor the turnover of GFP-tagged ATG4B and LC3B in living animal cells.The data show that ATG4B localizes to early autophagic membranes in an LC3B-dependent manner.During autophagy,ATG4B and LC3B undergo rapid cytosol/isolation membrane exchange but not at the cytosol/completed autophagosome.In addition,ATG4B activity controls the efficiency of autophagosome formation by impacting the membrane binding/dissociation of LC3B.These data suggest that ATG4 and LC3 play interdependent roles in the formation of autophagosomes.展开更多
Dear Editor,Most cancer cells maintain the length of their telomeres via telomerase(Günes and Rudolph,2013;Kim et al.,1994).However,in some cancers,telomeres are maintained not by telomerase but by alternative le...Dear Editor,Most cancer cells maintain the length of their telomeres via telomerase(Günes and Rudolph,2013;Kim et al.,1994).However,in some cancers,telomeres are maintained not by telomerase but by alternative lengthening of telomeres(Bryan et al.,1997).Telomerase activity appears to be reduced in some bone marrow(BM)diseases,including chronic myeloid leukemia,acute myeloid leukemia(AML),and myeloproliferative neoplasms(MPN),leading to shortened telomeres that correlate with leukemogenesis(Bouillon et al.,2018;Engelhardt et al.,2000;Shay et al.,1996).Our current study aimed to determine the definitive roles of telomeres and telomerase in leukemogenesis.展开更多
Dear Editor,Telomere attrition is one of the hallmark of aging.Lategeneration Terc knockout mice exhibit impaired hematopoiesis,1 while the underling mechanisms remain poorly understood.Retrotransposon long interspers...Dear Editor,Telomere attrition is one of the hallmark of aging.Lategeneration Terc knockout mice exhibit impaired hematopoiesis,1 while the underling mechanisms remain poorly understood.Retrotransposon long interspersed element-1(L1)is the only human retrotransposable elements capable of autonomous retrotransposition,and evolutionarily inactive.Recent studies reported that L1 is derepressed during the aging process with redistribution and reorganization of the heterochromatin.2 Considering that telomere shortening can cause chromosome instability and rearrangements,3 we speculate that L1 may play a role in impaired hematopoiesis in telomere dysfunctional mice.展开更多
Hematopoietic stem cells(HSCs)are responsible for generating all blood cells throughout life.Apart from the role of HSCs in maintaining the homeostasis of blood cell production process,they must respond quickly to hem...Hematopoietic stem cells(HSCs)are responsible for generating all blood cells throughout life.Apart from the role of HSCs in maintaining the homeostasis of blood cell production process,they must respond quickly to hematopoietic challenges,such as infection or blood loss.HSCs can be directly/indirectly activated and engage in blood formation for the acute needs in response to inflammation.Recent findings highlight the emerging role of inflammation signaling on HSC fate decision and shaping the hematopoietic system during aging.Here,we summarize recent studies identifying the changes in inflammation and their role in modulation of HSC function and discuss the interaction between inflammation and HSC biology in the contexts of aging and hematological malignancy.展开更多
Aberrant expression of circular RNAs(circ RNAs)is frequently linked to colorectal cancer(CRC).Here,we identified circ ZFR as a promising biomarker for CRC diagnosis and prognosis.Circ ZFR was upregulated in CRC tissue...Aberrant expression of circular RNAs(circ RNAs)is frequently linked to colorectal cancer(CRC).Here,we identified circ ZFR as a promising biomarker for CRC diagnosis and prognosis.Circ ZFR was upregulated in CRC tissues and serum exosomes and its level was linked to cancer incidence,advanced-stages,and metastasis.In both in vitro and in vivo settings,circ ZFR promoted the growth and spread while suppressing apoptosis of CRC.Exosomes with circ ZFR overexpression promoted the proliferation and migration of cocultured CRC cells.Mechanistically,epithelial splicing regulatory protein 1(ESRP1)in CRC cells may enhance the production of circ ZFR.BCL2-associated transcription factor 1(BCLAF1)bound to circ ZFR,which prevented its ubiquitinated degradation.Additionally,circ ZFR sponged mi R-3127-5p to boost rhotekin 2(RTKN2)expression.Our TCP1-CD-QDs nanocarrier was able to carry and deliver circ ZFR si RNA(si-circ ZFR)to the vasculature of CRC tissues and cells,which inhibited the growth of tumors in patient-derived xenograft(PDX)models.Taken together,our results show that circ ZFR is an oncogenic circ RNA,which promotes the development and spread of CRC in a BCLAF1 and mi R-3127-5p-dependent manner.Circ ZFR is a possible serum biopsy marker for the diagnosis and a desirable target for further treatment of CRC.展开更多
基金supported by the National Natural Science Foundation of China(31730036,31871380,31871382,31930055,31930058,32000500,32022034,32030033,32070730,32130046,3217050247,32150005,32200595,32222024,81730019,81730022,81830014,81921006,81925005,81970426,81971301,81971312,82030041,82061160495,82070805,82071595,82090020,82100841,82120108009,82122024,82125002,82125011,82125012,82130045,82171284,82173061,82173398,82225007,82225015,82225017,82225018,82230047,82230088,82271600,91949106,91949201,92049116,92049302,92049304,92149303,92149306,92157202,92168201,92169102,92249301,92268201)the National Key Research and Development Program of China(2018YFA0800700,2018YFC2000100,2018YFC2000102,2018YFC2002003,2019YFA0110900,2019YFA0801703,2019YFA0801903,2019YFA0802202,2019YFA0904800,2020YFA0113400,2020YFA0803401,2020YFA0804000,2020YFC2002900,2020YFC2008000,2020YFE0202200,2021YFA0804900,2021YFA1100103,2021YFA1100900,2021YFE0114200,2021ZD0202400,2022YFA0806001,2022YFA0806002,2022YFA0806600,2022YFA1103200,2022YFA1103601,2022YFA1103701,2022YFA1103800,2022YFA1103801,2022YFA1104100,2022YFA1104904,2022YFA1303000,2022YFC2009900,2022YFC2502401,2022YFC3602400,2022YFE0118000,2022ZD0213200)+14 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16030302,XDB39000000,XDB39030600)the Youth Innovation Promotion Association of Chinese Academy of Sciences(2020085,2021080)CAS Project for Young Scientists in Basic Research(YSBR-076)the Program of the Beijing Natural Science Foundation(JQ20031)Clinical Research Operating Fund of Central High level hospitals(2022-PUMCHE-001)CAMS Innovation Fund for Medical Sciences(CIFMS)(2022-I2M1-004)Talent Program of the Chinese Academy of Medical Science(2022RC310-10)Research Funds from Health@Inno HK Program launched by Innovation Technology Commission of the Hong Kong Special Administrative Region,Guangdong Basic and Applied Basic Research Foundation(2020B1515020044)Guangzhou Planned Project of Science and Technology(202002020039)the Major Technology Innovation of Hubei Province(2019ACA141)the Science and Technology Major Project of Hunan Provincial Science and Technology Department(2021SK1010)Shanghai Municipal Science and Technology Major Project(2017SHZDZX01)the Natural Science Foundation of Sichuan Province(2023NSFSC0003)Yunnan Fundamental Research Project(202201AS070080)the State Key Laboratory of Membrane Biology。
文摘Aging biomarkers are a combination of biological parameters to(i)assess age-related changes,(ii)track the physiological aging process,and(iii)predict the transition into a pathological status.Although a broad spectrum of aging biomarkers has been developed,their potential uses and limitations remain poorly characterized.An immediate goal of biomarkers is to help us answer the following three fundamental questions in aging research:How old are we?Why do we get old?And how can we age slower?This review aims to address this need.Here,we summarize our current knowledge of biomarkers developed for cellular,organ,and organismal levels of aging,comprising six pillars:physiological characteristics,medical imaging,histological features,cellular alterations,molecular changes,and secretory factors.To fulfill all these requisites,we propose that aging biomarkers should qualify for being specific,systemic,and clinically relevant.
基金supported by National Science Fund for Distinguished Young Scholars(82225025)National Natural Science Foundation of China(21877049,32171296,32201166,82172088)+2 种基金Guangdong Natural Science Foundation(2020B1515120043)Guangdong Basic and Applied Basic Research Fund Project(No.2021A1515111027)K.C.Wong Education Foundation.
文摘The therapeutic efficacy of radioimmunotherapy against triple negative breast cancer(TNBC)is largely limited by the complicated tumor microenvironment(TME)and its immunosuppressive state.Thus developing a strategy to reshape TME is expected to achieve highly efficient radioimmunotherapy.Therefore,we designed and synthesized a tellurium(Te)-driven maple leaf manganese carbonate nanotherapeutics(MnCO3@Te)by gas diffusion method,but also provided a chemical catalytic strategy in situ to augment ROS level and activate immune cells for improving cancer radioimmunotherapy.As expected,with the help of H2O2 in TEM,MnCO3@Te heterostructure with reversible Mn3+/Mn2+transition could catalyze the intracellular ROS overproduction to amplify radiotherapy.In addition,by virtue of the ability to scavenge H+in TME by carbonate group,MnCO3@Te directly promote the maturation of dendritic cells and macrophage M1 repolarization by stimulator of interferon genes(STING)pathway activation,resulting in remodeling immuno-microenvironment.As a result,MnCO3@Te synergized with radiotherapy and immune checkpoint blockade therapy effectively inhibited the breast cancer growth and lung metastasis in vivo.Collectively,these findings indicate that MnCO3@Te as an agonist,successfully overcome radioresistance and awaken immune systems,showing promising potential for solid tumor radioimmunotherapy.
基金supported by the National Key Research and Development Program of China(2017YFA0103304)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16010100)+5 种基金the National Key Research and Development Program of China(2015CB964800,2017YFA0102802,2014CB910503 and 2018YFA0107203)the National High Tech no logy Research and Development Program of China(2015AA020307)the National Natural Science Foundation of China(Grant Nos.31671429,91749202,91749123,81625009,81330008,81371342,81471414,81422017,81601233,81671377,31601109,31601158,81771515 and 81701388)Program of Beijing Municipal Science and Technology Commission(Z151100003 915072)Key Research Program of the Chinese Academy of Sciences(KJZDEW-TZ-L05),Beijing Municipal Commission of Health and Family Planning(PXM2018_026283_000002)Advanced Innovation Center for Human Brain Protection(117212).
文摘Aging increases the risk of various diseases. The main goal of aging research is to find therapies that attenuate aging and alleviate aging-related diseases. In this study, we screened a natural product library for geroprotective compounds using Werner syndrome (WS) human mesenchymal stem cells (hMSCs), a premature aging model that we recently established. Ten candidate compounds were identified and quercetin was investigated in detail due to its leading effects. Mechanistic studies revealed that quercetin alleviated senescence via the enhancement of cell proliferation and restoration of heterochromatin architecture in WS hMSCs. RNA-sequencing analysis revealed the transcriptional commonalities and differences in the geroprotective effects by quercetin and Vitamin C. Besides WS hMSCs, quercetin also attenuated cellular senescence in Hutchinson-Gilford progeria syndrome (HGPS) and physiological-aging hMSCs. Taken together, our study identifies quercetin as a geroprotective agent against accelerated and natural aging in hMSCs, providing a potential therapeutic intervention for treating age-associated disorders.
文摘Vascular cell functionality is critical to blood vessel homeostasis. Constitutive NF-κB activation in vascular cells results in chronic vascular inflammation, leading to various cardiovascular diseases. However, how NF-κB regulates human blood vessel homeostasis remains largely elusive. Here, using CRISPR/Cas9-mediated gene editing, we generated RelA knockout human embryonic stem cells (hESCs) and differentiated them into various vascular cell derivatives to study how NF- KS modulates human vascular cells under basal and inflammatory conditions. Multi-dimensional phenotypic assessments and transcriptomic analyses revealed that RelA deficiency affected vascular cells via modulatinginflammation, survival, vasculogenesis, cell differentia- tion and extracellular matrix organization in a cell type- specific manner under basal condition, and that RelA protected vascular cells against apoptosis and modu- lated vascular inflammatory response upon tumor necrosis factor a (TNFa) stimulation. Lastly, further evaluation of gene expression patterns in IKBo knockout vascular cells demonstrated that IKBa acted largely independent of RelA signaling. Taken together, our data reveal a protective role of NF-κB/ReiA in modulating human blood vessel homeostasis and map the human vascular transcriptomic landscapes for the discovery of novel therapeutic targets.
基金This work was supported by Grants 2016YFA0100602,2017YFA0103302,and 2018YFA0109300 from the National Key Research and Development Program of ChinaGrants 81525010,81770155,91749117,91749203,81901403,82030039,82022026,and 82071572 from the National Natural Science Foundation of China+3 种基金Grant 2C32003 from Opening Project of Key Laboratory of Integrative Chinese and Western Medicine for the Diagnosis and Treatment of Circulatory Diseases of Zhejiang ProvinceGrant 2019B151502008 from the Science Foundation for Distinguished Young Scholars of Guangdong ProvinceGrant 2018GZR110103002 from Innovative Team Program of Guangzhou Regenerative Medicine and Health Guangdong LaboratoryGrant 2017ZT07S347 from the Program for Guangdong Introducing Innovative and Enterpreneurial Teams.
文摘Remarkable progress in ageing research has been achieved over the past decades.General perceptions and experimental evidence pinpoint that the decline of physical function often initiates by cell senescence and organ ageing.Epigenetic dynamics and immunometabolic reprogramming link to the alterations of cellular response to intrinsic and extrinsic stimuli,representing current hotspots as they not only(re-)shape the individual cell identity,but also involve in cell fate decision.This review focuses on the present findings and emerging concepts in epigenetic,inflammatory,and metabolic regulations and the consequences of the ageing process.Potential therapeutic interventions targeting cell senescence and regulatory mechanisms,using state-of-the-art techniques are also discussed.
基金supported by National Natural Science Foundation of China(grant No.81571902,31872742).
文摘Background:Epidermal stem cells(EpSCs)that reside in cutaneous hair follicles and the basal layer of the epidermis are indispensable for wound healing and skin homeostasis.Little is known about the effects of photochemical activation on EpSC differentiation,proliferation and migration during wound healing.The present study aimed to determine the effects of photodynamic therapy(PDT)on wound healing in vivo and in vitro.Methods:We created mouse full-thickness skin resection models and applied 5-aminolevulinic acid(ALA)for PDT to the wound beds.Wound healing was analysed by gross evaluation and haematoxylin–eosin staining in vivo.In cultured EpSCs,protein expression was measured using flow cytometry and immunohistochemistry.Cell migration was examined using a scratch model;apoptosis and differentiation were measured using flow cytometry.Results:PDT accelerated wound closure by enhancing EpSC differentiation,proliferation and migration,thereby promoting re-epithelialization and angiogenesis.PDT inhibited inflammatory infiltration and expression of proinflammatory cytokines,whereas the secretion of growth factors was greater than in other groups.The proportion of transient amplifying cells was significantly greater in vivo and in vitro in the PDT groups.EpSC migration was markedly enhanced after ALAinduced PDT.Conclusions:Topical ALA-induced PDT stimulates wound healing by enhancing re-epithelialization,promoting angiogenesis as well as modulating skin homeostasis.This work provides a preliminary theoretical foundation for the clinical administration of topical ALA-induced PDT in skin wound healing.
基金supported by the National Natural Science Foundation of China(81930003,81420108001,81870007,81920108001)the Major Research Plan(91642202).
文摘Eosinophils are terminally differentiated cells derived from hematopoietic stem cells(HSCs)in the bone marrow.Several studies have confirmed the effective roles of eosinophils in asthmatic airway pathogenesis.However,their regulatory functions have not been well elucidated.Here,increased C-C chemokine ligand 6(CCL6)in asthmatic mice and the human orthologs CCL15 and CCL23 that are highly expressed in asthma patients are described,which are mainly derived from eosinophils.Using Cc/6 knockout mice,further studies revealed CCL6-dependent allergic airway inflammation and committed eosinophilia in the bone marrow following ovalbumin(OVA)challenge and identified a CCL6-CCR1 regulatory axis in hematopoietic stem cells(HSCs).Eosinophil differentiation and airway inflammation were remarkably decreased by the specific CCR1 antagonist BX471.Thus,the study identifies that the CCL6-CCR1 axis is involved in the crosstalk between eosinophils and HSCs during the development of allergic airway inflammation,which also reveals a potential therapeutic strategy for targeting G protein-coupled receptors(GPCRs)for future clinical treatment of asthma.
基金This work was supported by grants from the Natural Science Foundation of Zhejiang province(Y13H030005)to QS,National Natural Science Foundation of China(81400221)Hangzhou Normal University(PF14002004017)to XZ。
文摘Susceptibility of gastrointestinal dysmotility increases with age-associated colonic degeneration.A paucity of remedies reversing colonic degeneration per se hinders the fundamental relief of symptoms.Here we discovered the correlation between colon degeneration and altered nicotinamide adenine dinucleotide(NAD)level in aged mice.Compared to 3-month-old young controls,2-year-old mice showed a spectrum of degenerative colonic phenotypes and exhibited a significant elongated transit time and slowed stool frequency in the context of Lomotil-induced slow-transit constipation.Despite upregulated colonic tryptophan hydroxylases expression,serotonin release and expression of colon-predominant type IV serotonin receptor,reduced viability of interstitial cells of Cajal while enhanced aquaporins(Aqp1,3 and 11)led to a less colonic motility and increased luminal dehydration in aged mice.Notably,this colonic degeneration was accompanied with reduced key NAD^(+)-generating enzyme expression and lowered NAD^(+)/NADH ratio in aged colon.Three-month continuous administration of beta nicotinamide mononucleotide,a NAD^(+)precursor,elevated colonic NAD^(+)level and improved defecation in aged mice.In contrast,pharmacological inhibition of nicotinamide phosphoribosyltransferase,the rate-limiting enzyme for NAD^(+)biosynthesis,induced a reduction in colonic NAD content and impaired gastrointestinal function in young mice.Taken together,these findings suggest the beneficial effect of NAD^(+)in maintaining colonic homoeostasis and reactivating NAD^(+)biosynthesis may represent a promising strategy to counteract age-related gastrointestinal degeneration.
基金supported by the grants from Tianjin Government (Nos 07JCZDJC10600,08ZCKFSF03200 and 09ZCZDSF03800)the Ministry of Science and Technology of China (Nos 2008AA1011005,2008AA022311,2009CB521803,2009CB918900,2010CB944900 and 2010DFB30270)
文摘Embryonic stem cells (ESCs) are a potential source of generating transplantable hematopoietic stem and progenitor cells,which in turn can serve as "seed" cells for hematopoietic regeneration.In this study,we aimed to gauge the ability of mouse ESCs directly differentiating into hematopoietic cells in adult bone marrow (BM).To this end,we first derived a new mouse ESC line that constitutively expressed the green fluorescent protein (GFP) and then injected the ESCs into syngeneic BM via intra-tibia.The progeny of the transplanted ESCs were then analyzed at different time points after transplantation.Notably,however,most injected ESCs differentiated into non-hematopoietic cells in the BM whereas only a minority of the cells acquired hematopoietic cell surface markers.This study provides a strategy for evaluating the differentiation potential of ESCs in the BM micro-environment,thereby having important implications for the physiological maintenance and potential therapeutic applications of ESCs.
基金supported by the National Key R&D Program of China(No.2018YFB2201803)the National Natural Science Foundation of China(Nos.61821001,61901045,and 61625104)。
文摘The multimode fiber(MMF)has great potential to transmit high-resolution images with less invasive methods in endoscopy due to its large number of spatial modes and small core diameter.However,spatial modes crosstalk will inevitably occur in MMFs,which makes the received images become speckles.A conditional generative adversarial network(GAN)composed of a generator and a discriminator was utilized to reconstruct the received speckles.We conduct an MMF imaging experimental system of transmitting over 1 m MMF with a 50μm core.Compared with the conventional method of U-net,this conditional GAN could reconstruct images with fewer training datasets to achieve the same performance and shows higher feature extraction capability.
基金This work is supported by National Natural Science Foundation of China(91749203,81525010,91949125,81801372,82030039)Program for Guangdong Introducing Innovative and Enterpreneurial Teams(2017ZT07S347)+2 种基金Innovative Team Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory(2018GZR110103002)Natural Science Foundation of Zhejiang Province(LGD19H030001)Key Laboratory of Aging and Cancer Biology of Zhejiang province,School of medicine,Hangzhou Normal University.
文摘Dear Editor,Emerging evidences show close associations between miRNAs and liver regeneration.MiR-126 is known as a governor of vascular integrity and angiogenesis.MiR-126 is involved in the self-renewal of hematopoietic stem cells(HSCS)and leukemia stem cells.1,2 MiR-126 also plays a vital role in hepatocellular carcinogenesis,however,the functions of miR-126 in liver regeneration were still unclear.
基金supported by the National Natural Science Foundation of China(Grant No.81571380)the Natural Science Foundation of Zhejiang Province–China(Grant No.LY16H080009)+2 种基金supported by the National Natural Science Foundation of China(Grant Nos.81130074,81420108017,and 81525010)funded by the National Key R&D Plan from the Ministry of Science and Technology of China(Grant No.SQ2016ZY05002341)partially supported by the Deutsche Forschungsgemeinschaft(DFG),Germany
文摘Maintenance of tissue-specific stem cells is vital for organ homeostasis and organismal longevity.Hematopoietic stem cells(HSCs) are the most primitive cell type in the hematopoietic system.They divide asymmetrically and give rise to daughter cells with HSC identity(selfrenewal) and progenitor progenies(differentiation),which further proliferate and differentiate into full hematopoietic lineages.Mammalian ageing process is accompanied with abnormalities in the HSC self-renewal and differentiation.Transcriptional changes and epigenetic modulations have been implicated as the key regulators in HSC ageing process.The DNA damage response(DDR)in the cells involves an orchestrated signaling pathway,consisting of cell cycle regulation,cell death and senescence,transcriptional regulation,as well as chromatin remodeling.Recent studies employing DNA repair-deficient mouse models indicate that DDR could intrinsically and extrinsically regulate HSC maintenance and play important roles in tissue homeostasis of the hematopoietic system.In this review,we summarize the current understanding of how the DDR determines the HSC fates and finally contributes to organismal ageing.
基金the National Natural Science Foundation of China(31671434,31701203,81420108017,81525010,and 91749203)the National Key Research and Development Program of China(2016YFA0100602,2017YFA0103302,2020YFA0112404,and 2018YFC2000705)+1 种基金the Program for Guangdong Introducing Innovative and Entrepreneurial Teams(2017ZT07S347)the Fundamental Research Funds for the Central Universities(21617336).
文摘The biogenesis of autophagosomes provides the basis for macroautophagy to capture and degrade intracellular cargoes.Binding of the autophagy-related protein ATG8/LC3 to autophagic membranes is essential to autophagosome formation,which involves the specific and dynamic processing of ATG8/LC3 by cysteine protease ATG4.However,to date,the mechanism whereby ATG4 is recruited to the membranes,the interaction of ATG4 and ATG8/LC3 on the membranes,and its role in the growth of phagophore are not completely understood.Here,we used fluorescence recovery after photobleaching to monitor the turnover of GFP-tagged ATG4B and LC3B in living animal cells.The data show that ATG4B localizes to early autophagic membranes in an LC3B-dependent manner.During autophagy,ATG4B and LC3B undergo rapid cytosol/isolation membrane exchange but not at the cytosol/completed autophagosome.In addition,ATG4B activity controls the efficiency of autophagosome formation by impacting the membrane binding/dissociation of LC3B.These data suggest that ATG4 and LC3 play interdependent roles in the formation of autophagosomes.
基金supported by the National Key Research and Development Program of China(2016YFA0100600,2017YFA0103400)the National Natural Science Foundation of China(81421002,81730006,81430004,81870086,8181101081)+1 种基金CAMS Initiative for Innovative Medicine(2017-I2M-3-009,2016-I2M-1-017)the CAMS Fundamental Research Funds for Central Research Institutes(2018PT31005)。
文摘Dear Editor,Most cancer cells maintain the length of their telomeres via telomerase(Günes and Rudolph,2013;Kim et al.,1994).However,in some cancers,telomeres are maintained not by telomerase but by alternative lengthening of telomeres(Bryan et al.,1997).Telomerase activity appears to be reduced in some bone marrow(BM)diseases,including chronic myeloid leukemia,acute myeloid leukemia(AML),and myeloproliferative neoplasms(MPN),leading to shortened telomeres that correlate with leukemogenesis(Bouillon et al.,2018;Engelhardt et al.,2000;Shay et al.,1996).Our current study aimed to determine the definitive roles of telomeres and telomerase in leukemogenesis.
基金supported by Grants 2016YFA0100602,2017YFA0103302,2018YFA0109300 from the National Key Research and Development Program of ChinaGrants 81525010,91749203,81871116,81501214,91749117,81770155,and 81771502 from the National Natural Science Foundation of China+2 种基金Grants LQ14C070002 from the Natural Science Foundation of Zhejiang Province of ChinaGrant 2018GZR110103002 from Innovative Team Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory and Grant 2017ZT07S347 from the Program for Guangdong Introducing Innovative and Enterpreneurial Teamssupported by the Science Foundation for Distinguished Young Scholars of Guangdong Province(2019B151502008)to Hu Wang.
文摘Dear Editor,Telomere attrition is one of the hallmark of aging.Lategeneration Terc knockout mice exhibit impaired hematopoiesis,1 while the underling mechanisms remain poorly understood.Retrotransposon long interspersed element-1(L1)is the only human retrotransposable elements capable of autonomous retrotransposition,and evolutionarily inactive.Recent studies reported that L1 is derepressed during the aging process with redistribution and reorganization of the heterochromatin.2 Considering that telomere shortening can cause chromosome instability and rearrangements,3 we speculate that L1 may play a role in impaired hematopoiesis in telomere dysfunctional mice.
基金supported by grants 2016YFA0100602,2017YFA0103302 from the National Key Research and Development Programof ChinaGrants 81420108017,81525010,and 91749203 from and the National Natural Science Foundation of China+1 种基金the Program for Guangdong Introducing Innovative and Entrepreneurial Teams(2017ZT07S347)Innovative Team Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory(2018GZR110103002).
文摘Hematopoietic stem cells(HSCs)are responsible for generating all blood cells throughout life.Apart from the role of HSCs in maintaining the homeostasis of blood cell production process,they must respond quickly to hematopoietic challenges,such as infection or blood loss.HSCs can be directly/indirectly activated and engage in blood formation for the acute needs in response to inflammation.Recent findings highlight the emerging role of inflammation signaling on HSC fate decision and shaping the hematopoietic system during aging.Here,we summarize recent studies identifying the changes in inflammation and their role in modulation of HSC function and discuss the interaction between inflammation and HSC biology in the contexts of aging and hematological malignancy.
基金supported by the National Natural Science Foundation of China(81771502,82302899,32071349,81701820)the Natural Science Foundation of Zhejiang Province(LH19H160001,LY20H180014)the Department of Health of Zhejiang Province(2018KY473,2018PY025)。
文摘Aberrant expression of circular RNAs(circ RNAs)is frequently linked to colorectal cancer(CRC).Here,we identified circ ZFR as a promising biomarker for CRC diagnosis and prognosis.Circ ZFR was upregulated in CRC tissues and serum exosomes and its level was linked to cancer incidence,advanced-stages,and metastasis.In both in vitro and in vivo settings,circ ZFR promoted the growth and spread while suppressing apoptosis of CRC.Exosomes with circ ZFR overexpression promoted the proliferation and migration of cocultured CRC cells.Mechanistically,epithelial splicing regulatory protein 1(ESRP1)in CRC cells may enhance the production of circ ZFR.BCL2-associated transcription factor 1(BCLAF1)bound to circ ZFR,which prevented its ubiquitinated degradation.Additionally,circ ZFR sponged mi R-3127-5p to boost rhotekin 2(RTKN2)expression.Our TCP1-CD-QDs nanocarrier was able to carry and deliver circ ZFR si RNA(si-circ ZFR)to the vasculature of CRC tissues and cells,which inhibited the growth of tumors in patient-derived xenograft(PDX)models.Taken together,our results show that circ ZFR is an oncogenic circ RNA,which promotes the development and spread of CRC in a BCLAF1 and mi R-3127-5p-dependent manner.Circ ZFR is a possible serum biopsy marker for the diagnosis and a desirable target for further treatment of CRC.