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Temperature‑Arousing Self‑Powered Fire Warning E‑Textile Based on p-n Segment Coaxial Aerogel Fibers for Active Fire Protection in Firefighting Clothing 被引量:1
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作者 Hualing He Yi Qin +6 位作者 zhenyu zhu Qing Jiang Shengnan Ouyang Yuhang Wan Xueru Qu Jie Xu Zhicai Yu 《Nano-Micro Letters》 SCIE EI CAS CSCD 2023年第12期141-160,共20页
Firefighting protective clothing is a crucial protective equipment for firefighters to minimize skin burn and ensure safety firefighting operation and rescue mission.A recent increasing concern is to develop self-powe... Firefighting protective clothing is a crucial protective equipment for firefighters to minimize skin burn and ensure safety firefighting operation and rescue mission.A recent increasing concern is to develop self-powered fire warning materials that can be incorporated into the firefighting clothing to achieve active fire protection for firefighters before the protective clothing catches fire on fireground.However,it is still a challenge to facilely design and manufacture thermoelectric(TE)textile(TET)-based fire warning electronics with dynamic surface conformability and breathability.Here,we develop an alternate coaxial wet-spinning strategy to continuously produce alternating p/n-type TE aerogel fibers involving n-type Ti_(3)C_(2)T_(x)MXene and p-type MXene/SWCNT-COOH as core materials,and tough aramid nanofiber as protective shell,which simultaneously ensure the flexibility and high-efficiency TE power generation.With such alternating p/n-type TE fibers,TET-based self-powered fire warning sensors with high mechanical stability and wearability are successfully fabricated through stitching the alternating p-n segment TE fibers into aramid fabric.The results indicate that TET-based fire warning electronics containing 50 p-n pairs produce the open-circuit voltage of 7.5 mV with a power density of 119.79 nW cm-2 at a temperature difference of 300℃.The output voltage signal is then calculated as corresponding surface temperature of firefighting clothing based on a linear relationship between TE voltage and temperature.The fire alarm response time and flame-retardant properties are further displayed.Such self-powered fire warning electronics are true textiles that offer breathability and compatibility with body movement,demonstrating their potential application in firefighting clothing. 展开更多
关键词 Self-powered fire warning Coaxial wet spinning P-n segment thermoelectric fiber Thermoelectric textiles Active fire protection
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Numerical Simulation of Low Cycle Fatigue Behavior of Ti_(2)AlNb Alloy Subcomponents
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作者 Yanju Wang zhenyu zhu +1 位作者 Aixue Sha Wenfeng Hao 《Computer Modeling in Engineering & Sciences》 SCIE EI 2023年第9期2655-2676,共22页
Many titanium alloy subcomponents are subjected to fatigue loading in aerospace engineering,resulting in fatigue failure.The fatigue behavior of Ti_(2)AlNb alloy subcomponents was investigated based on the Seeger fati... Many titanium alloy subcomponents are subjected to fatigue loading in aerospace engineering,resulting in fatigue failure.The fatigue behavior of Ti_(2)AlNb alloy subcomponents was investigated based on the Seeger fatigue life theory and the improved Lemaitre damage evolution theory.Firstly,the finite element models of the standard openhole specimen and Y-section subcomponents have been established by ABAQUS.The damage model parameters were determined by fatigue tests,and the reliability of fatigue life simulation results of the Ti_(2)AlNb alloy standard open-hole specimen was verified.Meanwhile,the fatigue life of Ti_(2)AlNb alloy Y-section subcomponents was predicted.Under the same initial conditions,the average error of fatigue life predicted by two different models was 20.6%.Finally,the effects of loading amplitude,temperature,and Y-interface angle on fatigue properties of Ti_(2)AlNb Y-section subcomponents were investigated.These results provide a new idea for evaluating the fatigue life of various Ti_(2)AlNb alloy subcomponents. 展开更多
关键词 Fatigue life prediction stiffness degeneration Ti2AlNb alloy continuous damage mechanics numerical simulation
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Physical characteristics of high concentrated gas hydrate reservoir in the Shenhu production test area,South China Sea
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作者 Jiapeng JIN Xiujuan WANG +7 位作者 zhenyu zhu Pibo SU Lixia LI Qingping LI Yiqun GUO Jin QIAN Zhendong LUAN Jilin ZHOU 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第2期694-709,共16页
High concentrated and heterogeneous distribution of gas hydrates have been identified in the gas hydrate production test region in the Shenhu area,South China Sea.The gas hydrate-bearing sediments with high saturation... High concentrated and heterogeneous distribution of gas hydrates have been identified in the gas hydrate production test region in the Shenhu area,South China Sea.The gas hydrate-bearing sediments with high saturation locate at two ridges of submarine canyon with different thickness and saturations just above the bottom simulating reflection.The crossplots of gamma ray,acoustic impedance(P-impedance)and porosity at four sites show that the sediments can be divided into the upper and lower layers at different depths,indicating different geotechnical reservoir properties.Therefore,the depositional environments and physical properties at two ridges are analyzed and compared to show the different characteristics of hydrate reservoir.High porosity,high P-wave velocity,and coarse grain size indicate better reservoir quality and higher energy depositional environment for gas hydrate at Sites W18 and W19 than those at Sites W11 and W17.Our interpretation is that the base of canyon deposits at Sites W18 and W19 characterized by upward-coarsening units may be turbidity sand layers,thus significantly improving the reservoir quality with increasing gas hydrate saturation.The shelf and slope sliding deposits compose of the fine-grained sediments at Sites W11 and W17.The gas hydrate production test sites were conducted at the ridge of W11 and W17,mainly because of the thicker and larger area of gas hydrate-bearing reservoirs than those at Sites W18 and W19.All the results provide useful insights for assessing reservoir quality in the Shenhu area. 展开更多
关键词 reservoir properties gas hydrate production test region depositional environment South China Sea
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Effect of processing on the alkaloids in Aconitum tubers by HPLC-TOF/MS 被引量:11
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作者 Min Liu Yan Cao +4 位作者 Diya Lv Wen Zhang zhenyu zhu Hai Zhang Yifeng Chai 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2017年第3期170-175,共6页
According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and the effect of processing is unclear. This research aimed to explore the effect of processing on cardiac effica... According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and the effect of processing is unclear. This research aimed to explore the effect of processing on cardiac efficacy of alkaloids in Aconitum tubers. First, the chemical ingredients in unprocessed and processed Aconitum tubers were identified and compared by using high performance liquid chromatography time-of-flight mass spectrometry(HPLC-TOF/MS) and multivariate pattern recognition methods. Then the representative alkaloids in Aconitum tubers, aconitine, benzoylaconine, and aconine, which belong to diester-diterpenoid alkaloids,monoester-diterpenoid alkaloids, and amine-diterpenoid alkaloids, respectively, were selected for further validation of attenuated mechanism. Subsequent pharmacological experiments with aconitine, benzoylaconine,and aconine in SD rats were used to validate the effect of processing on cardiac functions. After processing the Aconitum tubers, it was found that the contents of diester-diterpenoid alkaloids were reduced, and those of monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids were increased, suggesting that diesterditerpenoid alkaloids were transformed into monoester-diterpenoid alkaloids and amine-diterpenoid alkaloids.Through further decocting the aconitine in boiling water, it was confirmed that the three alkaloids could be progressively transformed. Pharmacological experiments with aconitine, benzoylaconine, and aconine in SD rats showed that aconitine at a dose of 0.01 mg/kg and aconine at a dose of 10 mg/kg enhanced the cardiac function, while benzoylaconine at a dose of 2 mg/kg weakened the cardiac function. The effect of processing is attributed to the transformation of the most toxic diester-diterpenoid alkaloids into less toxic monoesterditerpenoid alkaloids and amine-diterpenoid alkaloids. 展开更多
关键词 ACONITUM TUBERS ALKALOIDS PROCESSING HPLC-TOF/MS
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Screening of immune cell activators from Astragali Radix using a comprehensive two-dimensional NK-92MI cell membrane chromatography/C18 column/time-of-flight mass spectrometry system 被引量:1
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作者 Xinyi Chai Yanqiu Gu +8 位作者 Lei Lv Chun Chen Fei Feng Yan Cao Yue Liu zhenyu zhu Zhanying Hong Yifeng Chai Xiaofei Chen 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2022年第5期725-732,共8页
Astragali Radix(AR)is a clinically used herbal medicine with multiple immunomodulatory activities that can strengthen the activity and cytotoxicity of natural killer(NK)cells.However,owing to the complexity of its com... Astragali Radix(AR)is a clinically used herbal medicine with multiple immunomodulatory activities that can strengthen the activity and cytotoxicity of natural killer(NK)cells.However,owing to the complexity of its composition,the specific active ingredients in AR that act on NK cells are not clear yet.Cell membrane chromatography(CMC)is mainly used to screen the active ingredients in a complex system of herbal medicines.In this study,a new comprehensive two-dimensional(2D)NK-92MI CMC/C18 column/time-of-flight mass spectrometry(TOFMS)system was established to screen for potential NK cell activators.To obtain a higher column efficiency,3-mercaptopropyltrimethoxysilane-modified silica was synthesized to prepare the NK-92MI CMC column.In total,nine components in AR were screened from this system,which could be washed out from the NK-92MI/CMC column after 10 min,and they showed good affinity for NK-92MI/CMC column.Two representative active compounds of AR,isoastragaloside Ⅰ and astragaloside IV,promoted the killing effect of NK cells on K562 cells in a dose-dependent manner.It can thus suggest that isoastragaloside Ⅰ and astragaloside Ⅳ are the main immunomodulatory components of AR.This comprehensive 2D NK-92MI CMC analytical system is a practical method for screening immune cell activators from other herbal medicines with immunomodulatory effects. 展开更多
关键词 NK cell activators Cell membrane chromatography Immunomodulatory herbal medicine Covalent bonding
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Development of a surface plasmon resonance biosensor for accurate and sensitive quantitation of small molecules in blood samples
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作者 Minyu Qi Diya Lv +7 位作者 Ying Zhang Dongyao Wang Xiaofei Chen zhenyu zhu Zhanying Hong Yifeng Chai Hai Zhang Yan Cao 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2022年第6期929-936,共8页
Therapeutic drug monitoring(TDM)has played an important role in clinical medicine for precise dosing.Currently,chromatographic technology and immunoassay detection are widely used in TDM and have met most of the needs... Therapeutic drug monitoring(TDM)has played an important role in clinical medicine for precise dosing.Currently,chromatographic technology and immunoassay detection are widely used in TDM and have met most of the needs of clinical drug therapy.However,some problems still exist in practical applications,such as complicated operation and the influence of endogenous substances.Surface plasmon resonance(SPR)has been applied to detect the concentrations of small molecules,including pesticide residues in crops and antibiotics in milk,which indicates its potential for in vivo drug detection.In this study,a new SPR-based biosensor for detecting chloramphenicol(CAP)in blood samples was developed and validated using methodological verification,including precision,accuracy,matrix effect,and extraction recovery rate,and compared with the classic ultra-performance liquid chromatographyultraviolet(UPLC-UV)method.The detection range of SPR was 0.1-50 ng/mL and the limit of detection was 0.099±0.023 ng/mL,which was lower than that of UPLC-UV.The intra-day and inter-day accuracies of SPR were 98%-114% and 110%-122%,which met the analysis requirement.The results show that the SPR biosensor is identical to UPLC-UV in the detection of CAP in rat blood samples;moreover,the SPR biosensor has better sensitivity.Therefore,the present study shows that SPR technology can be used for the detection of small molecules in the blood samples and has the potential to become a method for therapeutic drug monitoring. 展开更多
关键词 Surface plasmon resonance UPLC-UV CHLORAMPHENICOL Methodological verification
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动物消化道微生物多样性与宿主协同进化关系的研究进展
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作者 俄广旭 白天天 +1 位作者 朱振宇 郭雪峰 《生物多样性》 CAS CSCD 北大核心 2023年第11期202-221,共20页
动物消化道栖息着复杂的微生物群落,它们与动物的生理和代谢过程有着密切的联系。现有研究表明消化道微生物的演化与多样性受到动物生存环境、饮食习惯、生活方式等因素影响,而消化道微生物群落组成和功能也可以影响到动物的进化,这种... 动物消化道栖息着复杂的微生物群落,它们与动物的生理和代谢过程有着密切的联系。现有研究表明消化道微生物的演化与多样性受到动物生存环境、饮食习惯、生活方式等因素影响,而消化道微生物群落组成和功能也可以影响到动物的进化,这种寄生与被寄生物种间的相互作用是二者进化过程中微生物多样性的重要驱动力。本文概述了动物与消化道微生物进化关系及不同动物的消化道微生物群的组成,并探讨了动物与消化道微生物群在适应性进化过程中的联系以及不同动物中消化道优势微生物,我们发现尽管动物消化道微生物区系组成具有多样性,但大多数动物消化道70%–90%的微生物群落仍然来自于厚壁菌门和拟杆菌门,其原因可能是这两个菌门在分解复杂碳水化合物方面有重要作用。同时我们也指出当前研究应考虑到动物和消化道微生物进化趋势的定义、进化速度以及动物与消化道微生物间存在的竞争与合作等因素对于二者进化关系的影响,以便于验证其准确性。 展开更多
关键词 动物 消化道 消化道微生物区系 共同进化 多样性 优势菌门
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MatHub-2d:二维材料输运数据库及其高迁移率二维半导体材料高通量筛选应用 被引量:1
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作者 姚明佳 计嘉琳 +6 位作者 李鑫 朱振宇 葛军饴 David J.Singh 奚晋扬 杨炯 张文清 《Science China Materials》 SCIE EI CAS CSCD 2023年第7期2768-2776,共9页
近些年来二维材料因其独特的物理化学性质引起了广泛关注.载流子迁移率是材料在电子设备应用中最重要的特性之一.在本文中,我们介绍了如何通过高通量计算筛选来发现高迁移率二维半导体材料.基于最近开发的MatHub-2d数据库(包含约1900个... 近些年来二维材料因其独特的物理化学性质引起了广泛关注.载流子迁移率是材料在电子设备应用中最重要的特性之一.在本文中,我们介绍了如何通过高通量计算筛选来发现高迁移率二维半导体材料.基于最近开发的MatHub-2d数据库(包含约1900个二维材料的结构信息及其第一性原理计算结果),以带隙、磁性、弹性模量和形变势作为搜索标准,通过初步筛选,得到133个候选者.对这些体系,我们使用形变势方法和玻尔兹曼输运理论预测了迁移率.最终,我们预测19种二维材料在室温下(300 K)具有高迁移率(>10^(3)cm^(-2)V^(-1)s^(-1))和良好的稳定性.这些材料高迁移率的来源主要是较小的形变势常数、较大的弹性模量,以及较小的有效质量.其中有两种类型的化合物值得关注,BX(X=P,As,Sb)和ZO_(2)(Z=Ge,Sn,Pb),它们具有面内各向同性高迁移率.BX中“flower-like”化学键有利于p型和n型电输运,而Z-O反键态是ZO_(2)型二维材料良好电子传导的原因.除了这些二维材料,Si_(2)P_(2)、Ga_(2)O_(2)、Ge_(2)N_(2)等同样也表现出高的电子迁移率.这些高迁移率二维材料在新型半导体电子器件中具有潜在的应用前景. 展开更多
关键词 two-dimensional materials high-throughput computational screening MatHub-2d MOBILITY
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Chinese expert consensus on the overall management of liver function in conversion therapy for liver cancer(2022 edition)
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作者 Qinghua Meng Zhengqiang Yang +19 位作者 zhenyu zhu Juan Li Xinyu Bi Xiao Chen Chunyi Hao Zhen Huang Fei Li Xiao Li Guangming Li Yinmo Yang Yefan Zhang Haitao Zhao Hong Zhao Xu zhu Jiye zhu Jianqiang Cai The Liver Tumor Branch of the China International Exchange and Promotive Association for Medical and Healthcare(CPAM) The Gastrointestinal Cancer Multidisciplinary Cooperation Group of Cancer Hospital Chinese Academy of Medical Sciences&Peking Union Medical College The Liver Cancer Professional Committee of Chinese Medical Doctor Association 《Chinese Medical Journal》 SCIE CAS CSCD 2023年第24期2909-2911,共3页
Introduction Primary liver cancer is a significant health issue in China,where it ranks fourth in morbidity and second in mortality among all malignant tumors.[1-4]The 5-year overall survival rate of Chinese patients ... Introduction Primary liver cancer is a significant health issue in China,where it ranks fourth in morbidity and second in mortality among all malignant tumors.[1-4]The 5-year overall survival rate of Chinese patients with liver cancer from 2012 to 2015 was only 12.2%for males and 13.1%for females.[5]Hepatocellular carcinoma(HCC)accounts for 75-85%of primary liver cancers.[6]Furthermore,most liver cancers are unresectable at diagnosis.To combat this,conversion therapy is used,which involves systematic treatments like anti-angiogenic drugs or molecular targeted therapy combined with immunotherapies,as well as locoregional treatments such as transcatheter arterial chemoembolization(TACE),hepatic artery infusion chemotherapy(HAIC),ablation therapy,and radiation therapy.The goal is to eliminate unresectable status and increase the chances of surgery.The reported 5-year survival rate after salvage surgery following tumor downstaging varied from 24.9-57%. 展开更多
关键词 DRUGS INFUSION eliminate
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Synthesis and Structure of the Inclusion Compound COF300-ALA
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作者 Yuexian Hong Mingxuan Zhang +4 位作者 Zhipeng Zhou zhenyu zhu Jiaxing Lu Junliang Sun Zhikun Zheng 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2023年第22期2957-2962,共6页
Background and Originality Content,Inclusion single crystals(IsCs)are characterized by the encapsulation of guest molecules within the crystal lattice of a host framework,resulting in,well-defined channels or cavities... Background and Originality Content,Inclusion single crystals(IsCs)are characterized by the encapsulation of guest molecules within the crystal lattice of a host framework,resulting in,well-defined channels or cavities throughout the material. Host molecules can be broadly di.Videdinto wo main categories.i)The more widespreadgroup is unimolecular inclusion compounds where one host molecule interacts with one guest species.Familiar examples of this cate-gory include calixarenes,cyclodextrins,crown ethers,bai cavitands.cucurbituils,uoaciyclophanes,and various carcerands,ii) the other is multimoiecular inclusion caompounds where two or more host molecules are involved in forming cavities,channels,or layers in the crystal structure to accommodate guest molecules.Host molecules of,this kind include urea,choleic acis,Dianin's compound,etc.,and crystline porous materials such as metal-organic poliyhedrali and porous organic[18]cagesll are well-known examples. 展开更多
关键词 Covalent organic frameworks Inclusion compound Organic crystals
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胡焕庸线两侧城镇建设用地变化及其碳排放差异 被引量:22
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作者 张梅 黄贤金 +2 位作者 揣小伟 朱振宇 汪煜 《资源科学》 CSSCI CSCD 北大核心 2019年第7期1262-1273,共12页
城镇建设用地在利用和扩张过程中承载和拉动着大量的碳排放。揭示胡焕庸线东西两侧城镇建设用地的碳排放差异,并探讨其驱动因素,能为东西部的协调发展、碳减排工作和新型城镇化提供重要参考。本文借助夜间灯光遥感数据和GIS技术,提出了... 城镇建设用地在利用和扩张过程中承载和拉动着大量的碳排放。揭示胡焕庸线东西两侧城镇建设用地的碳排放差异,并探讨其驱动因素,能为东西部的协调发展、碳减排工作和新型城镇化提供重要参考。本文借助夜间灯光遥感数据和GIS技术,提出了边界阈值法,对胡焕庸线东西两侧的城镇建设用地进行了提取和分析,并综合相关文献中的碳排放估算方法对其所承载的碳排放进行估算和分析,在此基础上,对其关联性进行了剖析,并结合相关社会经济数据对研究结果进行深入讨论。研究表明:①边界阈值法提取结果的检验结果较好,优于经验阈值法和邻域分析法。②胡焕庸线存在被打破的可能性,2000—2013年东、西两侧城镇建设用地的面积比从95:5变为92:8,差异缩小。③西部地区的快速发展是以高碳排放为代价的,2000—2013年东、西两侧年碳排放量比值从93:7变为90:10,差异缩小。④东、西两侧的城镇建设用地每增加1 km2时,年均碳排放量分别增长1.79万t和2.58万t。因此,国家在继续重视碳排放量较大的东部地区的同时,也应重视西部地区加剧的高碳排放问题。可通过引导城镇建设用地的集约式扩张、优化产业结构和提倡绿色低碳的生活方式等手段促使西部地区走上集约低碳的新型城镇化道路。 展开更多
关键词 胡焕庸线 城镇建设用地 碳排放 时空差异 边界阈值法 夜间灯光遥感数据
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Chinese expert consensus on conversion therapy for hepatocellular carcinoma(2021 edition) 被引量:9
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作者 Hui-Chuan Sun Jian Zhou +77 位作者 Zheng Wang Xiufeng Liu Qing Xie Weidong Jia Ming Zhao Xinyu Bi Gong Li Xueli Bai Yuan Ji Li Xu Xiao-Dong zhu Dousheng Bai Yajin Chen Yongjun Chen Chaoliu Dai Rongping Guo Wenzhi Guo Chunyi Hao Tao Huang Zhiyong Huang Deyu Li Gang Li Tao Li Xiangcheng Li Guangming Li Xiao Liang Jingfeng Liu Fubao Liu Shichun Lu Zheng Lu Weifu Lv Yilei Mao Guoliang Shao Yinghong Shi Tianqiang Song Guang Tan Yunqiang Tang Kaishan Tao Chidan Wan Guangyi Wang Lu Wang Shunxiang Wang Tianfu Wen Baocai Xing Bangde Xiang Sheng Yan Dinghua Yang Guowen Yin Tao Yin zhenyu Yin Zhengping Yu Bixiang Zhang Jialin Zhang Shuijun Zhang Ti Zhang Yamin Zhang Yubao Zhang Aibin Zhang Haitao Zhao Ledu Zhou Wu Zhang zhenyu zhu Shukui Qin Feng Shen Xiujun Cai Gaojun Teng Jianqiang Cai Minshan Chen Qiang Li Lianxin Liu Weilin Wang Tingbo Liang Jiahong Dong Xiaoping Chen Xuehao Wang Shusen Zheng Jia Fan 《Hepatobiliary Surgery and Nutrition》 SCIE 2022年第2期227-252,I0011-I0014,共30页
Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected pa... Recent advances in systemic and locoregional treatments for patients with unresectable or advanced hepatocellular carcinoma(HCC)have resulted in improved response rates.This has provided an opportunity for selected patients with initially unresectable HCC to achieve adequate tumor downstaging to undergo surgical resection,a‘conversion therapy’strategy.However,conversion therapy is a new approach to the treatment of HCC and its practice and treatment protocols are still being developed.Review the evidence for conversion therapy in HCC and develop consensus statements to guide clinical practice.Evidence review:Many research centers in China have accumulated significant experience implementing HCC conversion therapy.Preliminary findings and data have shown that conversion therapy represents an important strategy to maximize the survival of selected patients with intermediate stage to advanced HCC;however,there are still many urgent clinical and scientific challenges for this therapeutic strategy and its related fields.In order to summarize and learn from past experience and review current challenges,the Chinese Expert Consensus on Conversion Therapy for Hepatocellular Carcinoma(2021 Edition)was developed based on a review of preliminary experience and clinical data from Chinese and non-Chinese studies in this field and combined with recommendations for clinical practice.Sixteen consensus statements on the implementation of conversion therapy for HCC were developed.The statements generated in this review are based on a review of clinical evidence and real clinical experience and will help guide future progress in conversion therapy for patients with HCC. 展开更多
关键词 Hepatocellular carcinoma(HCC) conversion therapy surgical resection systematic treatment locoregional treatment CONSENSUS China
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In situ synthesis and unidirectional insertion of membrane proteins in liposome-immobilized silica stationary phase for rapid preparation of microaffinity chromatography 被引量:2
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作者 Yanqiu Gu Rong Wang +11 位作者 Panpan Chen Shengnan Li Xinyi Chai Chun Chen Yue Liu Yan Cao Diya Lv Zhanying Hong zhenyu zhu Yifeng Chai Yongfang Yuan Xiaofei Chen 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2022年第9期3682-3693,共12页
Cell membrane affinity chromatography has been widely applied in membrane protein(MP)-targeted drug screening and interaction analysis.However,in current methods,the MP sources are derived from cell lines or recombina... Cell membrane affinity chromatography has been widely applied in membrane protein(MP)-targeted drug screening and interaction analysis.However,in current methods,the MP sources are derived from cell lines or recombinant protein expression,which are time-consuming for cell culture or purification,and also difficult to ensure the purity and consistent orientation of MPs in the chromatographic stationary phase.In this study,a novel in situ synthesis membrane protein affinity chromatography(iSMAC)method was developed utilizing cell-free protein expression(CFE)and covalent immobilized affinity chromatography,which achieved efficient in situ synthesis and unidirectional insertion of MPs into liposomes in the stationary phase.The advantages of iSMAC are:1)There is no need to culture cells or prepare recombinant proteins;2)Specific and purified MPs with stable and controllable content can be obtained within 2 h;3)MPs maintain the transmembrane structure and a consistent orientation in the chromatographic stationary phase;4)The flexible and personalized construction of cDNAs makes it possible to analyze drug binding sites.iSMAC was successfully applied to screen PDGFRβinhibitors from Salvia miltiorrhiza and Schisandra chinensis.Micro columns prepared by in-situ synthesis maintain satisfactory analysis activity within 72 h.Two new PDGFRβinhibitors,salvianolic acid B and gomisin D,were screened out with KD values of 13.44 and 7.39μmol/L,respectively.In vitro experiments confirmed that the two compounds decreasedα-SMA and collagen Ⅰ mRNA levels raised by TGF-βin HSC-T6 cells through regulating the phosphorylation of p38,AKT and ERK.In vivo,Sal B could also attenuate CCl_(4)-induced liver fibrosis by downregulating PDGFRβdownstream related protein levels.The iSMAC method can be applied to other general MPs,and provides a practical approach for the rapid preparation of MP-immobilized or other biological solid-phase materials. 展开更多
关键词 Affinity chromatography In situ synthesis of membrane proteins Unidirectional insertion PDGFRβ Drug screening Ligand–protein interaction Antihepatic fibrosis
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Activation of the cold-sensing TRPM8 channel triggers UCP1-dependent thermogenesis and prevents obesity 被引量:2
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作者 Shuangtao Ma Hao Yu +12 位作者 Zhigang Zhao Zhidan Luo Jing Chen Yinxing Ni Rongbing Jin Liqun Ma Peijian Wang zhenyu zhu Li Li Jian Zhong Daoyan Liu Bernd Nilius Zhiming zhu 《Journal of Molecular Cell Biology》 SCIE CAS CSCD 2012年第2期88-96,共9页
Brown adipose tissue(BAT)is an energy-expending organ that produces heat.Expansion or activation of BAT prevents obesity and diabetes.Chronic cold exposure enhances thermogenesis in BAT through uncoupling protein 1(UC... Brown adipose tissue(BAT)is an energy-expending organ that produces heat.Expansion or activation of BAT prevents obesity and diabetes.Chronic cold exposure enhances thermogenesis in BAT through uncoupling protein 1(UCP1)activation triggered via a b-adrenergic pathway.Here,we report that the cold-sensing transient receptor potential melastatin 8(TRPM8)is functionally present in mouse BAT.Challenging brown adipocytes with menthol,a TRPM8 agonist,up-regulates UCP1 expression and requires protein kinase A activation.Upon mimicking long-term cold exposure with chronic dietary menthol application,menthol significantly increased the core temperatures and locomotor activity in wild-type mice;these effects were absent in both TRPM82/2 and UCP12/2 mice.Dietary obesity and glucose abnormalities were also prevented by menthol treatment.Our results reveal a previously unrecognized role for TRPM8,suggesting that stimulation of this channel mediates BAT thermogenesis,which could constitute a promising way to treat obesity. 展开更多
关键词 TRPM8 uncoupling protein 1 MENTHOL THERMOGENESIS brown adipose tissue OBESITY
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High thermoelectric properties with low thermal conductivity due to the porous structure induced by the dendritic branching in n-type PbS 被引量:2
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作者 zhenyu zhu Janak Tiwari +3 位作者 Tianli Feng Zhan Shi Yue Lou Biao Xu 《Nano Research》 SCIE EI CSCD 2022年第5期4739-4746,共8页
PbS-based thermoelectric materials have attracted extensive attention in recent years for the advantages of earth abundancy and low cost,which is considered to be a substitute for traditional PbTe material.However,the... PbS-based thermoelectric materials have attracted extensive attention in recent years for the advantages of earth abundancy and low cost,which is considered to be a substitute for traditional PbTe material.However,their high thermal conductivity restricts its development.Hence,in order to improve their thermoelectric performance from reducing the thermal conductivity,a kind of dendritic PbS with controlled crystal grain and morphology are obtained by solution synthesis.By adjusting the amount of surfactant(CTAB),the specific formation process of dendrites is regulated.After sintering,the dendritic PbS nanoparticles are easy to form porous structure due to the overlapping and staggered arrangement of dendritic branches.For comparison,we also prepare a kind of regular octahedral PbS and a dense packing arrangement is formed because of the integrity of the octahedral structure.DFT-based Boltzmann transport equation is used to prove the crucial role of porous structure in scattering phonon.Finally,a maximum zT=1.0 at 773 K in n-type PbS is obtained,which still keep a high-speed growth and is expected to get higher zT value in a higher temperature region.Our work may shed light to other thermoelectric materials from the formation of porous structure to reduce the thermal conductivity. 展开更多
关键词 thermoelectric materials lead sulfide solution synthesis porous structure
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Evolutionary Game-theoretic Analysis for Residential Users Considering Integrated Demand Response 被引量:1
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作者 Bingtuan Gao Chen Chen +2 位作者 Yanhui Qin Xiaofeng Liu zhenyu zhu 《Journal of Modern Power Systems and Clean Energy》 SCIE EI CSCD 2021年第6期1500-1509,共10页
As an important part of demand side,residential users have the characteristics of imperfect rationality and strong randomness,which are rarely considered in the existing study.Moreover,to effectively improve the energ... As an important part of demand side,residential users have the characteristics of imperfect rationality and strong randomness,which are rarely considered in the existing study.Moreover,to effectively improve the energy efficiency,integrated demand response(IDR)is proposed as an effective measure to reduce the local energy supply pressure.This paper focuses on a scenario for IDR programs,in which the intelligent building aggregator(IBA)wants to encourage residential users to participate in IDR according to a proper contract price policy.To analyze how the participation degree tendency evolves over time,an evolutionary game approach is proposed considering residential users’bounded-rationality.A symmetric evolutionary game model and an asymmetric evolutionary game model are established,and the stability of equilibrium points in the above models is proven.Simulation results show that different contract price policies will obviously influence residential users’strategy,and affect the stable equilibrium points of the evolutionary game.The simulation results provide an effective reference for IBA to set proper and effective price incentives. 展开更多
关键词 Contract price evolutionary game intelligent community energy consumption behavior integrated demand response(IDR)
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Elimination of inter-domain interactions increases the cleavage fidelity of the restriction endonuclease Dralll 被引量:1
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作者 Wei zhuo Xuhui Lai +5 位作者 Liqing Zhang Siu-Hong Chan Fengjuan Li zhenyu zhu Maojun Yang Dapeng Sun 《Protein & Cell》 SCIE CAS CSCD 2014年第5期357-368,共12页
Dralll is a type liP restriction endonucleases (REases) that recognizes and creates a double strand break within the gapped palindromic sequence CACTNNN^GTG of double-stranded DNA indicates nicking on the bottom st... Dralll is a type liP restriction endonucleases (REases) that recognizes and creates a double strand break within the gapped palindromic sequence CACTNNN^GTG of double-stranded DNA indicates nicking on the bottom strand; indicates nicking on the top strand). However, wild type Dralll shows significant star activity. In this study, it was found that the prominent star site is CATSGTT;GTG, consisting of a star 5' half (CAT) and a canonical 3' half (GTG). Dralll nicks the 3' canonical half site at a faster rate than the 5' star half site, in contrast to the similar rate with the canonical full site. The crystal structure of the Dralll protein was solved. It indicated, as supported by mutagenesis, that Dralll possesses a ~13a- metal HNH active site. The structure revealed extensive intra-molecular interactions between the N-terminal domain and the C-terminal domain containing the HNH active site. Disruptions of these interactions through site- directed mutagenesis drastically increased cleavage fidelity. The understanding of fidelity mechanisms will enable generation of high fidelity REases. 展开更多
关键词 Dralll restriction endonuclease fidelity substrate specificity star activity inter-domain interactionsite-directed mutagenesis
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Accurate and explainable machine learning for the power factors of diamond-like thermoelectric materials 被引量:1
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作者 Zhe Yang Ye Sheng +5 位作者 Cong zhu Jianyue Ni zhenyu zhu Jinyang Xi Wu Zhang Jiong Yang 《Journal of Materiomics》 SCIE 2022年第3期633-639,共7页
The application of machine learning(ML)-based methods to the study of thermoelectric(TE)materials is promising.Although conventional ML algorithms can achieve high prediction performance,their lack of interpretability... The application of machine learning(ML)-based methods to the study of thermoelectric(TE)materials is promising.Although conventional ML algorithms can achieve high prediction performance,their lack of interpretability severely obstructs researchers from extracting material-oriented insights from ML models.In this work,high ML-based prediction performance was achieved with respect to TE power factors(PFs),and the results were well understood by the SHapley Additive exPlanations(SHAP),a method to identify the correlations between targets and descriptors.We designed a robust PF prediction model for diamond-like compounds via a stacking technique,and the model achieved a coefficient of determination value above 0.95 on the test set.From the SHAP analysis,the PFs were negatively correlated with electronegativity and positively correlated with the descriptor“volume per atom”based on the previously reported dataset.TE domain knowledge was adopted to understand these correlations.This work shows that ML models can achieve high accuracy while exhibiting good interpretability,making them useful for materials scientists. 展开更多
关键词 Machine learning Thermoelectric materials INTERPRETABILITY Power factor
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Screening potential P-glycoprotein inhibitors by combination of a detergent-free membrane protein extraction with surface plasmon resonance biosensor
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作者 Yuhong Cao Jiahao Fang +7 位作者 Yiwei Shi Hui Wang Xiaofei Chen Yue Liu zhenyu zhu Yan Cao Zhanying Hong Yifeng Chai 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2022年第7期3113-3123,共11页
P-glycoprotein(P-gp)highly expressed in cancer cells can lead to multidrug resistance(MDR)and the combination of anti-cancer drugs with P-gp inhibitor has been a promising strategy to reverse MDR in cancer treatment.I... P-glycoprotein(P-gp)highly expressed in cancer cells can lead to multidrug resistance(MDR)and the combination of anti-cancer drugs with P-gp inhibitor has been a promising strategy to reverse MDR in cancer treatment.In this study,we established a label-free and detergent-free system combining surface plasmon resonance(SPR)biosensor with styrene maleic acid(SMA)polymer membrane proteins(MPs)stabilization technology to screen potential P-gp inhibitors.First,P-gp was extracted from MCF-7/ADR cells using SMA polymer to form SMA liposomes(SMALPs).Following that,SMALPs were immobilized on an SPR biosensor chip to establish a P-gp inhibitor screening system,and the affinity between P-gp and small molecule ligand was determined.The methodological investigation proved that the screening system had good specificity and stability.Nine P-gp ligands were screened out from 50 natural products,and their affinity constants with P-gp were also determined.The in vitro cell verification experiments demonstrated that tetrandrine,fangchinoline,praeruptorin B,neobaicalein,and icariin could significantly increase the sensitivity of MCF-7/ADR cells to Adriamycin(Adr).Moreover,tetrandrine,praeruptorin B,and neobaicalein could reverse MDR in MCF-7/ADR cells by inhibiting the function of P-gp.This is the first time that SMALPs-based stabilization strategy was applied to SPR analysis system.SMA polymer can retain P-gp in the environment of natural lipid bilayer and thus maintain the correct conformation and physiological functions of P-gp.The developed system can quickly and accurately screen small molecule ligands of complex MPs and obtain affinity between complex MPs and small molecule ligands without protein purification. 展开更多
关键词 Styrene maleic acid P-GLYCOPROTEIN Surface plasmon resonance Membrane proteins Inhibitor screening Affinity calculation Natural products Multidrug resistance
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硼酸盐:氧离子导体设计的一个新方向
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作者 李晓辉 杨利 +10 位作者 朱振宇 王晓鸽 陈柏桦 黄森传 魏先益 蔡国鸿 Manuel Pascal 杨四海 林建华 匡小军 孙俊良 《Science China Materials》 SCIE EI CAS CSCD 2022年第10期2737-2745,共9页
降低固体氧化物燃料电池(SOFCs)的工作温度正在推动新型氧离子导体材料的开发.在此,受氧离子导体材料结构共性的启发,基于Sr(Si/Ge)O_(3)材料中刚性(Si/Ge)_(3)O_(9)基团无法容纳氧空位,以及BO_(n)多面体在配位数、旋转、变形和连接上... 降低固体氧化物燃料电池(SOFCs)的工作温度正在推动新型氧离子导体材料的开发.在此,受氧离子导体材料结构共性的启发,基于Sr(Si/Ge)O_(3)材料中刚性(Si/Ge)_(3)O_(9)基团无法容纳氧空位,以及BO_(n)多面体在配位数、旋转、变形和连接上的巨大灵活性,我们首次通过计算预测和实验验证,报道了基于硼酸盐的新型氧离子导体材料(Gd/Y)_(1−x)-Zn_(x)BO_(3−0.5x).(Gd/Y)BO_(3)中的氧空位可以通过孤立的B_(3)O_(9)三元环形成B_(3)O_(8)结构单元来容纳,并通过B_(3)O_(9)和B_(3)O_(8)单元之间的氧交换合作机制来传输,而这些结构单元作为过渡态的打开和伸展则有助于氧的传输.这项研究为设计和开发新型氧离子导体开辟了新方向,有望从硼酸盐家族发现更多新的氧离子导体. 展开更多
关键词 氧离子导体 硼酸盐 结构单元 配位数 合作机制 过渡态 氧空位 结构共性
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