Monomethyl auristatin E(MMAE)is a derivative of the marine peptide Dolastatin 10,which has therapeutic effects against various cancers according to its antimitotic activity in multiple clinical trials.The antibody dru...Monomethyl auristatin E(MMAE)is a derivative of the marine peptide Dolastatin 10,which has therapeutic effects against various cancers according to its antimitotic activity in multiple clinical trials.The antibody drug conjugate(ADC)of MMAE is currently used in clinical practice.However,the safety issues of MMAE-based ADC,such as high drug toxicity and poor bioavailability,still exist when using it for anticancer therapy.A sustained release of drug delivery approach should be used to reduce toxicity and achieve sufficient anticancer effects.Herein,PLGA-b-PEG 2000 with excellent biocompatibility and slow degradation ability was adopted to construct MMAE-loaded nanoparticles for safe and effective chemotherapy.The sustained release effect and the immunogenic cell death(ICD)effect of PLGA-MMAE nanoparticles were assessed by in vitro experiments.The PLGA-MMAE nanoparticles effectively accumulated in the tumor through the enhanced permeability and retention(EPR)effect,inducing cell apoptosis and causing a certain degree of immune response.The sustained drug release of PLGA-MMAE improved the bioavailability and effectively reduced the toxicity and development of the tumor compared to the effect of free MMAE or ADC.Overall,this study provides a safe and effective chemotherapeutic approach,as well as a simple and effective synthetic process for MMAE-based nanoparticles,improving their therapeutic efficacy and safety.展开更多
Objective:To systematically evaluate the effect of modified length of gastric tube implantation in stroke patients.Methods:Randomized controlled studies on the effect of narrative therapy on negative emotions of malig...Objective:To systematically evaluate the effect of modified length of gastric tube implantation in stroke patients.Methods:Randomized controlled studies on the effect of narrative therapy on negative emotions of malignant tumor patients were published in PubMed,EMbase,Web of Science,Cochrane Library,CNKY.Wanfang Data,VIP Database and CBM disc from database establishment to May 2021.RewMan 5.2 software was used for the meta-analysis.Results:Ten articles were included,involving 1140 patients in total.Results of meta-analysis showed that improved length of gastric tube implantation could reduce the incidence of aspiration pneumonia in stroke patients with enteral nutrition[OR=0.18,95%CI(0.10,0.31),P<0.00001],reduce gastroesophageal reflux rate[OR=0.13,95%CI(0.04,0.38),P<0.0002],the incidence of aspiration[OR=0.23,95%CI(0.11,0.38),P<0.00001],the incidence of abdominal distension and diarrhea[OR=0.13,95%CI(0.06,0.28),P<0.00001],the incidence of choking cough[OR=0.20,95%CI(0.07,0.61),P<0.005],but no difference between the two groups in terms of reducing gastric residual[OR=0.10,95%CI(2.40,2.60),P=0.94].Conclusions:The modified length of the gastric tube can replace the traditional length of the gastric tube in the application of stroke patients,can reduce the complications of enteral nutrition,is safe and feasible.However,due to the limitation of the quality and quantity of the included studies,we should be cautious about the results of this meta-analysis and expect to carry out more large-sample and high-quality randomized controlled trials for demonstration.展开更多
基金funded by the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(No.820LH027)the Hainan Provincial Natural Science Foundation of China(No.823RC472)+1 种基金the Open Project Program of Wuhan National Laboratory for Optoelectronics(No.2021WNLOKF008)the Hainan University Scientific Research Foundation(KYQD(ZR)19107).
文摘Monomethyl auristatin E(MMAE)is a derivative of the marine peptide Dolastatin 10,which has therapeutic effects against various cancers according to its antimitotic activity in multiple clinical trials.The antibody drug conjugate(ADC)of MMAE is currently used in clinical practice.However,the safety issues of MMAE-based ADC,such as high drug toxicity and poor bioavailability,still exist when using it for anticancer therapy.A sustained release of drug delivery approach should be used to reduce toxicity and achieve sufficient anticancer effects.Herein,PLGA-b-PEG 2000 with excellent biocompatibility and slow degradation ability was adopted to construct MMAE-loaded nanoparticles for safe and effective chemotherapy.The sustained release effect and the immunogenic cell death(ICD)effect of PLGA-MMAE nanoparticles were assessed by in vitro experiments.The PLGA-MMAE nanoparticles effectively accumulated in the tumor through the enhanced permeability and retention(EPR)effect,inducing cell apoptosis and causing a certain degree of immune response.The sustained drug release of PLGA-MMAE improved the bioavailability and effectively reduced the toxicity and development of the tumor compared to the effect of free MMAE or ADC.Overall,this study provides a safe and effective chemotherapeutic approach,as well as a simple and effective synthetic process for MMAE-based nanoparticles,improving their therapeutic efficacy and safety.
文摘Objective:To systematically evaluate the effect of modified length of gastric tube implantation in stroke patients.Methods:Randomized controlled studies on the effect of narrative therapy on negative emotions of malignant tumor patients were published in PubMed,EMbase,Web of Science,Cochrane Library,CNKY.Wanfang Data,VIP Database and CBM disc from database establishment to May 2021.RewMan 5.2 software was used for the meta-analysis.Results:Ten articles were included,involving 1140 patients in total.Results of meta-analysis showed that improved length of gastric tube implantation could reduce the incidence of aspiration pneumonia in stroke patients with enteral nutrition[OR=0.18,95%CI(0.10,0.31),P<0.00001],reduce gastroesophageal reflux rate[OR=0.13,95%CI(0.04,0.38),P<0.0002],the incidence of aspiration[OR=0.23,95%CI(0.11,0.38),P<0.00001],the incidence of abdominal distension and diarrhea[OR=0.13,95%CI(0.06,0.28),P<0.00001],the incidence of choking cough[OR=0.20,95%CI(0.07,0.61),P<0.005],but no difference between the two groups in terms of reducing gastric residual[OR=0.10,95%CI(2.40,2.60),P=0.94].Conclusions:The modified length of the gastric tube can replace the traditional length of the gastric tube in the application of stroke patients,can reduce the complications of enteral nutrition,is safe and feasible.However,due to the limitation of the quality and quantity of the included studies,we should be cautious about the results of this meta-analysis and expect to carry out more large-sample and high-quality randomized controlled trials for demonstration.