Aptamer-Based Cell-Surface Profiling with Single-Cell Resolution Enables Precise Cancer Characterization

Molecular profiling of cell-surface proteins is a powerful strategy for precise cancer diagnosis.While mass cytometry(MC)enables synchronous detection of over 40 cellular parameters,its full potential in disease classification is challenged by the limited types of recognition probes currently available.In this work,we synthesize a panel of heavy isotopeconjugated aptamers to profile cancer-associated signatures on the surface of hematological malignancy(HM)cells.Based on 15 molecular signatures,we performed cell-surface profiling that allowed the precise classification of 8 HM cell lines.Combined with machine-learning technology,this aptamer-based MC platform also achieved multiclass identification of HM subtypes in clinical sampleswith 100%accuracy in the training cohort and 80%accuracy in the test cohort.Therefore,we report an effective and practical strategy for precise cancer classification at the singlecell level,paving the way for its clinical use in the near future.

Mild synthesis of ultra-bright carbon dots with solvatochromism for rapid lipid droplet monitoring in varied physiological processes

Lipid droplets(LDs)participating in various cellular activities and are increasingly being emphasized.Fluorescence imaging provides powerful tool for dynamic tracking of LDs,however,most current LDs probes remain inconsistent performance such as low Photoluminescence Quantum Yield(PLQY),poor photostability and tedious washing procedures.Herein,a novel yellow-emissive carbon dot(OT-cD)has been synthesized conveniently with high PLQY up to 90%.Besides,OT-CD exhibits remarkable amphiphilicity and solvatochromic property with lipid-water partition coefficient higher than 2,which is much higher than most LDs probes.These characters enable OT-CD high brightness,stable and wash-free LDs probing,and feasible for in vivo imaging.Then,detailed observation of LDs morphological and polarity variation dynamically in different cellular states were recorded,including ferroptosis and other diseases processes.Furthermore,fast whole imaging of zebrafish and identifed LD enrichment in injured liver indicate its further feasibility for in vivo application.In contrast to the reported studies to date,this approach provides a versatile conventional synthesis system for high-performance LDs targeting probes,combing the advantages of easy and high-yield production,as well as robust brightness and stability for long-term imaging,facilitating investigations into organelle interactions and LD-associated diseases.

Wet-spinning fabrication of shear-patterned alginate hydrogel microfibers and the guidance of cell alignment

Native tissue is naturally comprised of highly-ordered cell-matrix assemblies in a multi-hierarchical way,and the nano/submicron alignment of fibrous matrix is found to be significant in supporting cellular functionalization.In this study,a self-designed wet-spinning device appended with a rotary receiving pool was used to continuously produce shear-patterned hydrogel microfibers with aligned submicron topography.The process that the flow-induced shear force reshapes the surface of hydrogel fiber into aligned submicron topography was systematically analysed.Afterwards,the effect of fiber topography on cellular longitudinal spread and elongation was investigated by culturing rat neuron-like PC12 cells and human osteosarcoma MG63 cells with the spun hydrogel microfibers,respectively.The results suggested that the stronger shear flow force would lead to more distinct aligned submicron topography on fiber surface,which could induce cell orientation along with fiber axis and therefore form the cell-matrix dual-alignment.Finally,a multi-hierarchical tissue-like structure constructed by dual-oriented cell-matrix assemblies was fabricated based on this wet-spinning method.This work is believed to be a potentially novel biofabrication scheme for bottom-up constructing of engineered linear tissue,such as nerve bundle,cortical bone,muscle and hepatic cord.

Three-dimensional flower-like SnS_(2)-supported bimetallic zeolite imidazole compound with enhanced electrocatalytic activity for methanol oxidation

Proton exchange membrane fuel cells(PEMFCs)are considered as one of the most effective technologies to resolve environmental problems and energy crisis for future automotive applications[1-4].The direct methanol fuel cell(DMFC)[5-6]is a new type of PEMFC using methanol as fuel[7].Because of its higher energy density and conversion efficiency,methanol can be used as direct fuel at a relatively lower operating temperature[8]with portable great potential in equipment and transportation utilization.Therefore,developing DMFCs that use methanol aqueous solution or steam methanol as fuel is a good choice to solve energy problems[9].

A review of hydrogels used in endoscopic submucosal dissection for intraoperative submucosal cushions and postoperative management

Endoscopic submucosal dissection(ESD)has been clinically proved to have prominent advantages in the treatment of early gastrointestinal cancers over traditional surgery,including less trauma,fewer complications,a quicker recovery and lower costs.During the procedure of ESD,appropriate and multifunctional submucosal injected materials(SIMs)as submucosal cushions play an important role,however,even with many advances in design strategies of SIMs over the past decades,the performance of the submucosal cushions with postoperative management function seems to be still unsatisfactory.In this review,we gave a brief historical recount about the clinical development of SIMs,then some common applications of hydrogels used as SIMs in ESD were summarized,while an account of the universal challenges during ESD procedure was also outlined.Going one step further,some cutting-edge functional strategies of hydrogels for novel applications in ESD were exhibited.Finally,we concluded the advantages of hydrogels as SIMs for ESD as well as the treatment dilemma clinicians faced when it comes to deeply infiltrated lesions,some technical perspectives about linking the clinical demand with commercial supply were also proposed.Encompassing the basic elements of SIMs used in ESD surgery and the corresponding postoperative management requirements,this review could be a good reference for relevant practitioners in expanding the research horizon and improving the well-being index of patients.