Effect of pharmacological intervention on MIP-1α,MIP-1β and MCP-1 expression in patients with psoriasis vulgaris

Objective:To detect the expression level of macrophage inflammatory protein-1(MIP-1)α,MIP-1βand monocyte chemoattractant protein-1(MCP-1)in with psoriasis vulgaris and explore the role in the pathogenesis of psoriasis vulgaris.Methods:The level of MIP-1α,MIP-1βand MCP-1 in peripheral blood from 50 patients with psoriasis vulgaris and 50 normal controls were measured by enzyme linked immunosorbent assay.The correlation with psoriasis area and severity index(PASI)was analyzed.The level of MIP-1α,MIP-1βand MCP-1 was compared between psoriasis vulgaris patients at active stage and resting stage.And the change in MIP-1α,MIP-1βand MCP-1 before and after therapy was also observed.Results:The content of MIP-1α,MIP-1βand MCP-1 in patients with psoriasis vulgaris was(1342.78±210.30),(175.28±28.18)and(266.86±32.75)ng/L,respectively,significantly higher than those in control group(P<0.05).The expression level of MIP-1α,MIP-1βand MCP-1 in peripheral blood of patients with psoriasis vulgaris was positively correlated wilh PASI(P<0.01).After acitretin therapy,expression level of MIP-1α,MIP-1βand MCP-1 in peripheral blood of patients with psoriasis vulgaris was significantly decreased.Conclusions:Chemokine factor MIP-1α,MIP-1βand MCP-1 may be involved in the pathogenesis of psoriasis vulgaris.

Effect of Yinxieling decoction on PASI,TNF-α and IL-8 in patients with psoriasis vulgaris

Objective:To study the effect of Yinxieling decoction on PASI,TNF-α and IL-8 in patients with psoriasis vulgaris.Methods:A total of 120 cases of psoriasis vulgaris were divided into 4 groups according to syndrome differentiation of TCM and randomized controlled method:wind heat syndrome group(group A).blood stasis syndrome group(group B) blood dryness syndrome group(group C)nd control group(group D)(n=30 per group).Patients in observation groups were treated with Yinxieling decoction,while patients in control group were treated by placebo for 8 weeks.Levels of TNT-α and IL-8 were determined before treatment.4 and 8 weeks after treatment.psoriasis area and severity index score was also performed before and after treatment.Results:psoriasis area and severity index score and serum level of TNF-α,IL-8 were significantly decreased in all groups.The decrease in three observation groups was more significant(P<0.05 or P<0.01),and the decrease in wind heat syndrome group was the most significant(P<0.01).psoriasis area and severity index was positively correlated with TNF-α and IT.-8,respectively(P<0.05).Conclusions:Yinxieling decoction has therapeutical effect on psoriasis vulgaris via regulating TNF-α and IL-8.

Ultrasonic findings of papillary thyroid carcinoma and its relationship with osteopontin expression

Objective: The ultrasonic findings of papillary thyroid carcinoma were analyzed by acoustic power pulse imaging, and the relationship between the expression of osteopontin and the expression of osteopontin was analyzed. Methods: Using color Doppler ultrasound in patients with lesions, morphology, internal echo, calcification and blood flow of the situation, and then switch to ARFI VTI mode and VTQ mode, VTI were measured with two-dimensional area and area ratio of VTQ mode SWV;osteopontin expression was detected in two groups of patients with thyroid tissues. Analysis of difference between the two the group index by using SPSS18.0 software, Pearson linear correlation method is used to analyze the area ratio and SWV value of thyroid tissue expression correlated with OPN. Results: The shape of papillary thyroid carcinoma nodules showed that the boundary was irregular and the shape of the nodules was burr. The shape of benign nodules was more than 1 with low echo in the interior. The shape of benign nodules showed clear boundary and middle hyperechoic in the interior. No calcification occurred in most lesions in that observation group, the area ratio in VTI mode is obviously higher than that of the control group, the difference was statistically significant (T=7.313, P=0.000), the SWV value in the VTQ model of the observation group was also significantly higher than that in the control group, the difference was statistically significant (t=43.334, P=0.000). The expression of OPNmRNA in the observation group was significantly higher than that of the control group , and the difference was statistically significant (t=8.894, P=0.000). Pearson linear correlation analysis showed that there was a significant positive correlation between OPNmRNA expression in papillary thyroid carcinoma and area ratio in VTI model and SWV course in VTQ model (r=0.235, 0.264;P<0.05). Conclusion: It is important for the diagnosis of papillary thyroid carcinoma to use the technique of acoustic power pulse imaging combined with the detection of OPNmRNA expression. It can be used as a new method for the diagnosis and prognosis of papillary thyroid carcinoma.