Objective:This study was performed to explore the conversion of adipose-derived stem cells(ADSCs)into sweat gland-like cells for the purpose of sweat gland regeneration.Methods:ADSCs and human sweat gland(hSG)cells we...Objective:This study was performed to explore the conversion of adipose-derived stem cells(ADSCs)into sweat gland-like cells for the purpose of sweat gland regeneration.Methods:ADSCs and human sweat gland(hSG)cells were isolated,cultured,and identified.The ADSCs were then cultured in combination with epidermal growth factor and/or cocultured with hSG cells in a Transwell coculturing system to transform the ADSCs into hSG-like cells.Phenotypic changes of the ADSCs were examined by morphological observation and immunocytochemical analysis of specific markers.Results:The ADSCs showed sweat gland-like morphologic changes and expressed sweat gland markers(cytokeratins 7,14,and 18).Conclusion:These findings revealed that ADSCs can differentiate into hSG-like cells after coculture in a Transwell system and that epidermal growth factor can enhance the efficiency of differentiation.ADSCs may serve as a potential source of cells for sweat gland regeneration.展开更多
基金This study was supported by the National Natural Science Fund of China(Nos.81271711 and 81471796).
文摘Objective:This study was performed to explore the conversion of adipose-derived stem cells(ADSCs)into sweat gland-like cells for the purpose of sweat gland regeneration.Methods:ADSCs and human sweat gland(hSG)cells were isolated,cultured,and identified.The ADSCs were then cultured in combination with epidermal growth factor and/or cocultured with hSG cells in a Transwell coculturing system to transform the ADSCs into hSG-like cells.Phenotypic changes of the ADSCs were examined by morphological observation and immunocytochemical analysis of specific markers.Results:The ADSCs showed sweat gland-like morphologic changes and expressed sweat gland markers(cytokeratins 7,14,and 18).Conclusion:These findings revealed that ADSCs can differentiate into hSG-like cells after coculture in a Transwell system and that epidermal growth factor can enhance the efficiency of differentiation.ADSCs may serve as a potential source of cells for sweat gland regeneration.
