AIM:To investigate the protective effect of human umbilical cord mesenchymal stem cells(hUCMSCs)modified by the LIF gene on the retinal function of diabetic model rats and preliminarily explore the possible mechanism....AIM:To investigate the protective effect of human umbilical cord mesenchymal stem cells(hUCMSCs)modified by the LIF gene on the retinal function of diabetic model rats and preliminarily explore the possible mechanism.METHODS:A stably transfected cell line of hUCMSCs overexpressing leukemia inhibitory factor(LIF)was constructed.Overexpression was verified by fluorescent quantitative polymerase chain reaction(qPCR).Forty-eight adult Sprague-Dawley rats were randomly divided into a normal control group(group A),streptozotocin-induced diabetic control group(group B),diabetic rats at 3mo injected with empty vector-transfected hUCMSCs(group C)or injected with LIF-hUCMSCs(group D).Four weeks after the intravitreal injection,analyses in all groups included retinal function using flash electroretinogram(F-ERG),retinal blood vessel examination of retinal flat mounts perfused with fluorescein isothiocyanate-dextran(FITCdextran),and retinal structure examination of sections using hematoxylin and eosin staining.Expression levels of adiponectin(APN),high-sensitivity C-reactive protein(hsCRP),and neurotrophin-4(NT-4)in each group was detected using immunohistochemistry,PCR,Western blotting,and ELISA,respectively.RESULTS:A stable transgenic cell line of LIF-hUCMSCs was constructed.F-ERG and FITC-dextran examinations revealed no abnormalities of retinal structure and function in group A,severe damage of the retinal blood vessels and function in group B,and improved retinal structure and function in group C and especially group D.qPCR,ELISA,and Western blot analyses revealed progressively higher APN and NT-4 expression levels in groups B,C,and D than in group A.hs-CRP expression was significantly higher in group B than in groups A,C,and D,and was significantly higher in group C than in group D(P<0.05).CONCLUSION:LIF-hUCMSCs protect the retina of diabetic rats by upregulating APN and NT-4 expression and downregulating hs-CRP expression in the retina.展开更多
A new method to access sulfonated dibenz[b,e]azepines via visible-light photoredox catalysis is described.Emplo ying inexpe nsive and commercially available sulfonyl chlorides to form the sulfonyl radicals enables the...A new method to access sulfonated dibenz[b,e]azepines via visible-light photoredox catalysis is described.Emplo ying inexpe nsive and commercially available sulfonyl chlorides to form the sulfonyl radicals enables the alkyne addition reaction followed by a 7-membered radical cyclization step to furnish sulfonated dibenzazepines.展开更多
A post-Ugi/diastereoselective cascade reaction was developed to construct naturally existing spiroindolines via a facile and metal-free one-pot protocol.During the construction,a new C-C bond was formed through a sele...A post-Ugi/diastereoselective cascade reaction was developed to construct naturally existing spiroindolines via a facile and metal-free one-pot protocol.During the construction,a new C-C bond was formed through a selective 5-exo-dig indole cyclization on the propiolamide;and a new C-N bond was diastereoselectively formed which controlled the configuration of three chiral centers simultaneously.展开更多
基金Tianjin Science and Technology Project(No.14JCYBJC27400)。
文摘AIM:To investigate the protective effect of human umbilical cord mesenchymal stem cells(hUCMSCs)modified by the LIF gene on the retinal function of diabetic model rats and preliminarily explore the possible mechanism.METHODS:A stably transfected cell line of hUCMSCs overexpressing leukemia inhibitory factor(LIF)was constructed.Overexpression was verified by fluorescent quantitative polymerase chain reaction(qPCR).Forty-eight adult Sprague-Dawley rats were randomly divided into a normal control group(group A),streptozotocin-induced diabetic control group(group B),diabetic rats at 3mo injected with empty vector-transfected hUCMSCs(group C)or injected with LIF-hUCMSCs(group D).Four weeks after the intravitreal injection,analyses in all groups included retinal function using flash electroretinogram(F-ERG),retinal blood vessel examination of retinal flat mounts perfused with fluorescein isothiocyanate-dextran(FITCdextran),and retinal structure examination of sections using hematoxylin and eosin staining.Expression levels of adiponectin(APN),high-sensitivity C-reactive protein(hsCRP),and neurotrophin-4(NT-4)in each group was detected using immunohistochemistry,PCR,Western blotting,and ELISA,respectively.RESULTS:A stable transgenic cell line of LIF-hUCMSCs was constructed.F-ERG and FITC-dextran examinations revealed no abnormalities of retinal structure and function in group A,severe damage of the retinal blood vessels and function in group B,and improved retinal structure and function in group C and especially group D.qPCR,ELISA,and Western blot analyses revealed progressively higher APN and NT-4 expression levels in groups B,C,and D than in group A.hs-CRP expression was significantly higher in group B than in groups A,C,and D,and was significantly higher in group C than in group D(P<0.05).CONCLUSION:LIF-hUCMSCs protect the retina of diabetic rats by upregulating APN and NT-4 expression and downregulating hs-CRP expression in the retina.
基金We gratefully ack no wledge the National Natural Scie nee Foundation of China(No.21901027)the Science and Technology Research Program of Chongqing Mun icipal Educati on Commissi on(Nos.KJQN201901345 and KJQN201901346)+1 种基金the Chongqing Natural Science Foundation Postdoctoral Science Foundation Project(No.cstc2019jcyj-bshX0053)the Scientific Research Founda-tion of the Chongqing University of Arts and Sciences(No.R2019FXY11)。
文摘A new method to access sulfonated dibenz[b,e]azepines via visible-light photoredox catalysis is described.Emplo ying inexpe nsive and commercially available sulfonyl chlorides to form the sulfonyl radicals enables the alkyne addition reaction followed by a 7-membered radical cyclization step to furnish sulfonated dibenzazepines.
基金The authors would like to thank the Science and Technology Research Program of Chongqing Municipal Education Commission(CXQT19029 and KJZD-M201801301)Science&Technology Department of Sichuan Province(2017FZ0084)the Natural Science Foundation Project of CQ CSTSC(cstc2018jszx-cyzdX0023 and cstc2021jcyj-bsh0233).We would also like to thank Ms H.Z.Liu and J.Xu for obtaining the HRMS and NMR data.
文摘A post-Ugi/diastereoselective cascade reaction was developed to construct naturally existing spiroindolines via a facile and metal-free one-pot protocol.During the construction,a new C-C bond was formed through a selective 5-exo-dig indole cyclization on the propiolamide;and a new C-N bond was diastereoselectively formed which controlled the configuration of three chiral centers simultaneously.
