AIM:To investigate the expression of Ras homolog(Rho)C,vascular endothelial growth factor(VEGF) and CD105 in esophageal squamous cell carcinoma.METHODS:Semi-quantitative reverse transcriptase polymerase chain reaction...AIM:To investigate the expression of Ras homolog(Rho)C,vascular endothelial growth factor(VEGF) and CD105 in esophageal squamous cell carcinoma.METHODS:Semi-quantitative reverse transcriptase polymerase chain reaction,in situ hybridization and immunohistochemical streptavidin-biotin- peroxidase methods were used to detect expression of Rho C m RNA and protein,and VEGF protein in 62 cases with esophageal squamous cell carcinoma,31 cases with adjacent atypical hyperplastic tissues,and 62 cases with normal esophageal mucosa.CD105 antibody labeling was used to measure microvascular density.Expression levels were compared according to clinicopathologic and patient parameters.RESULTS:Expression of Rho C m RNA showed a positive correlation with the protein level in esophageal squamous cell carcinoma,as well as with VEGF protein levels.Rho C m RNA expression was mainly located within the cytoplasm of the tumor cells,appearing as blue to purple particles by in situ hybridization.The differences in Rho C m RNA expression in esophageal squamous cell carcinoma,adjacent atypical hyperplasia and normal esophageal mucosa were significant(P < 0.05).The relative expression of Rho C m RNA in cancer tissues with lymph node metastasis was significantly higher than in the tissues without lymph node metastasis(P < 0.05).VEGF protein expression was consistent with microvascular density(t = 25.52,P < 0.05).Positive expression of VEGF protein in esophageal squamous cell carcinoma of different histologic gradings did not differ significantly.Positive expression of VEGF protein in carcinoma tissues with deep infiltration was significantly higher than in tissues with only superficial infiltration(P < 0.05).The positive expression of VEGF protein in cancer tissues with lymph node metastasis was significantly higher than in the tissues without lymph node metastasis(P < 0.05).CONCLUSION:Rho C protein may upregulate VEGF expression,thereby promoting tumor angiogenesis.Rho C m RNA and protein expression was correlated with metastasis.展开更多
Schwannomas are rarely observed in the gastrointestinal tract.The most common symptoms of a gastric schwannoma are abdominal pain or dyspepsia,gastrointestinal bleeding,and an abdominal mass.Many gastric schwannomas a...Schwannomas are rarely observed in the gastrointestinal tract.The most common symptoms of a gastric schwannoma are abdominal pain or dyspepsia,gastrointestinal bleeding,and an abdominal mass.Many gastric schwannomas are asymptomatic and are discovered incidentally or at postmortem.The diagnosis of a schwannoma is based on immunohistochemical positivity for S-100 protein.Wepresent a case report of a rare complication of gastric schwannoma causing gastroduodenal intussusception that was successfully managed by a BillrothⅡdistal gastrectomy.In this rare case,the patient had intermittent,colicky abdominal pain,nausea,and vomiting for over 4wk accompanied by a weight loss.A diagnosis of gastric intussusception was made by computed tomography.A BillrothⅡdistal gastrectomy was then performed,and complete en bloc removal(R0 resection)was achieved.Pathology confirmed a gastric schwannoma through positive immunohistochemical staining for S-100 protein.展开更多
Objective:To study the effect of trimetazidine combined with bisoprolol on the cardiac function, ventricular remodeling and neuroendocrine factors in patients with chronic heart failure.Methods: A total of 52 patients...Objective:To study the effect of trimetazidine combined with bisoprolol on the cardiac function, ventricular remodeling and neuroendocrine factors in patients with chronic heart failure.Methods: A total of 52 patients with chronic heart failure who were treated in our hospital between January 2012 and November 2015 were collected and divided into the control group (n=26) who received bisoprolol therapy and the observation group (n=26) who received trimetazidine combined with bisoprolol therapy according to the double-blind randomized control method, and both groups were treated for 3 months. Before treatment and after 3 months of treatment, cardiac color Doppler diasonograph was used to determine the levels of cardiac function parameters and ventricular remodeling parameters, and RIA method was used to determine the levels if peripheral blood neuroendocrine factors.Results: Before treatment, the differences in cardiac function, ventricular remodeling and neuroendocrine factor levels were not statistically significant between two groups of patients. After 3 months of treatment, cardiac function parameters LVEDd and LVESD levels of observation group were lower than those of control group while LVEF level was higher than that of control group, and ventricular remodeling parameters LVPWT, IVSS, PWD, PWS and LVMI levels were lower than those of control group;peripheral blood neuroendocrine factors NE, ALD, AngⅡ, ANP and ET contents of observation group were lower than those of control group.Conclusion:Trimetazidine combined with bisoprolol can optimize the cardiac function, suppress the ventricular remodeling process and regulate the neuroendocrine factor secretion in patients with chronic heart failure, and it contributes to the patients' overall optimization.展开更多
Objective:The aim of this study was to investigate the biological effects of occupational extremely low-frequency electromagnetic field(ELF-EMF)exposure on the thyroid gland.Methods:We conducted a prospective analysis...Objective:The aim of this study was to investigate the biological effects of occupational extremely low-frequency electromagnetic field(ELF-EMF)exposure on the thyroid gland.Methods:We conducted a prospective analysis of 85 workers(exposure group)exposed to an ELF-EMF(100μT,10-100 Hz)produced by the electromagnetic aircraft launch system and followed up on thyroid function indices,immunological indices,and color Doppler images for 3 years.Additionally,116 healthy volunteers were randomly selected as controls(control group),the thyroid function of whom was compared to the exposure group.Results:No significant difference was observed in thyroid function between the exposure and control groups.During the follow-up of the exposure group,the serum free triiodothyronine(FT3)level was found to slowly decrease and free thyroxine(FT4)level slowly increase with increasing exposure time.However,no significant difference was found in thyroid-stimulating hormone(TSH)over the three years,and no significant difference was observed in the FT3,FT4 and TSH levels between different exposure subgroups.Furthermore,no significant changes were observed in thyroid autoantibody levels and ultrasound images between subgroups or over time.Conclusion:Long-term exposure to ELF-EMF may promote thyroid secretion of T4 and inhibit deiodination of T4 to T3.ELF-EMF has no significant effect on thyroid immune function and morphology.展开更多
Objective:To explore the expressions of IL-1β and COX-2 in the gingiva tissues in rat periodontitis model with different estrogen levels, and the effect of estrogen level on periodontitis.Methods:A total of 40 female...Objective:To explore the expressions of IL-1β and COX-2 in the gingiva tissues in rat periodontitis model with different estrogen levels, and the effect of estrogen level on periodontitis.Methods:A total of 40 female Wistar rats were randomized into 4 groups, i.e. normal control group (n=10), periodontitis group (n=10), castraction periodontitis group (n=10), and estrogen therapy group (n=10). RT-PCR was used to detect the expressions of IL-1β and COX-2 in the gingiva tissues in each group.Results:The expression intensity of IL-1β and COX-2 in the estrogen therapy group was significantly lower than that in the castraction periodontitis group (P<0.05).Conclusions:Estrogen can significantly down regulate the expressions of IL-1β and COX-2 in order to alleviate the symptoms of periodontitis.展开更多
基金Supported by First Affiliated Hospital of Zhengzhou University,Henan-based Research Projects with Cutting-Edge Technology,No.132300410126 and No.132300410458
文摘AIM:To investigate the expression of Ras homolog(Rho)C,vascular endothelial growth factor(VEGF) and CD105 in esophageal squamous cell carcinoma.METHODS:Semi-quantitative reverse transcriptase polymerase chain reaction,in situ hybridization and immunohistochemical streptavidin-biotin- peroxidase methods were used to detect expression of Rho C m RNA and protein,and VEGF protein in 62 cases with esophageal squamous cell carcinoma,31 cases with adjacent atypical hyperplastic tissues,and 62 cases with normal esophageal mucosa.CD105 antibody labeling was used to measure microvascular density.Expression levels were compared according to clinicopathologic and patient parameters.RESULTS:Expression of Rho C m RNA showed a positive correlation with the protein level in esophageal squamous cell carcinoma,as well as with VEGF protein levels.Rho C m RNA expression was mainly located within the cytoplasm of the tumor cells,appearing as blue to purple particles by in situ hybridization.The differences in Rho C m RNA expression in esophageal squamous cell carcinoma,adjacent atypical hyperplasia and normal esophageal mucosa were significant(P < 0.05).The relative expression of Rho C m RNA in cancer tissues with lymph node metastasis was significantly higher than in the tissues without lymph node metastasis(P < 0.05).VEGF protein expression was consistent with microvascular density(t = 25.52,P < 0.05).Positive expression of VEGF protein in esophageal squamous cell carcinoma of different histologic gradings did not differ significantly.Positive expression of VEGF protein in carcinoma tissues with deep infiltration was significantly higher than in tissues with only superficial infiltration(P < 0.05).The positive expression of VEGF protein in cancer tissues with lymph node metastasis was significantly higher than in the tissues without lymph node metastasis(P < 0.05).CONCLUSION:Rho C protein may upregulate VEGF expression,thereby promoting tumor angiogenesis.Rho C m RNA and protein expression was correlated with metastasis.
基金Supported by Department of General Surgery,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai,China
文摘Schwannomas are rarely observed in the gastrointestinal tract.The most common symptoms of a gastric schwannoma are abdominal pain or dyspepsia,gastrointestinal bleeding,and an abdominal mass.Many gastric schwannomas are asymptomatic and are discovered incidentally or at postmortem.The diagnosis of a schwannoma is based on immunohistochemical positivity for S-100 protein.Wepresent a case report of a rare complication of gastric schwannoma causing gastroduodenal intussusception that was successfully managed by a BillrothⅡdistal gastrectomy.In this rare case,the patient had intermittent,colicky abdominal pain,nausea,and vomiting for over 4wk accompanied by a weight loss.A diagnosis of gastric intussusception was made by computed tomography.A BillrothⅡdistal gastrectomy was then performed,and complete en bloc removal(R0 resection)was achieved.Pathology confirmed a gastric schwannoma through positive immunohistochemical staining for S-100 protein.
文摘Objective:To study the effect of trimetazidine combined with bisoprolol on the cardiac function, ventricular remodeling and neuroendocrine factors in patients with chronic heart failure.Methods: A total of 52 patients with chronic heart failure who were treated in our hospital between January 2012 and November 2015 were collected and divided into the control group (n=26) who received bisoprolol therapy and the observation group (n=26) who received trimetazidine combined with bisoprolol therapy according to the double-blind randomized control method, and both groups were treated for 3 months. Before treatment and after 3 months of treatment, cardiac color Doppler diasonograph was used to determine the levels of cardiac function parameters and ventricular remodeling parameters, and RIA method was used to determine the levels if peripheral blood neuroendocrine factors.Results: Before treatment, the differences in cardiac function, ventricular remodeling and neuroendocrine factor levels were not statistically significant between two groups of patients. After 3 months of treatment, cardiac function parameters LVEDd and LVESD levels of observation group were lower than those of control group while LVEF level was higher than that of control group, and ventricular remodeling parameters LVPWT, IVSS, PWD, PWS and LVMI levels were lower than those of control group;peripheral blood neuroendocrine factors NE, ALD, AngⅡ, ANP and ET contents of observation group were lower than those of control group.Conclusion:Trimetazidine combined with bisoprolol can optimize the cardiac function, suppress the ventricular remodeling process and regulate the neuroendocrine factor secretion in patients with chronic heart failure, and it contributes to the patients' overall optimization.
基金supported by.the National Military Research Project of China(No.JGXM201507).
文摘Objective:The aim of this study was to investigate the biological effects of occupational extremely low-frequency electromagnetic field(ELF-EMF)exposure on the thyroid gland.Methods:We conducted a prospective analysis of 85 workers(exposure group)exposed to an ELF-EMF(100μT,10-100 Hz)produced by the electromagnetic aircraft launch system and followed up on thyroid function indices,immunological indices,and color Doppler images for 3 years.Additionally,116 healthy volunteers were randomly selected as controls(control group),the thyroid function of whom was compared to the exposure group.Results:No significant difference was observed in thyroid function between the exposure and control groups.During the follow-up of the exposure group,the serum free triiodothyronine(FT3)level was found to slowly decrease and free thyroxine(FT4)level slowly increase with increasing exposure time.However,no significant difference was found in thyroid-stimulating hormone(TSH)over the three years,and no significant difference was observed in the FT3,FT4 and TSH levels between different exposure subgroups.Furthermore,no significant changes were observed in thyroid autoantibody levels and ultrasound images between subgroups or over time.Conclusion:Long-term exposure to ELF-EMF may promote thyroid secretion of T4 and inhibit deiodination of T4 to T3.ELF-EMF has no significant effect on thyroid immune function and morphology.
文摘Objective:To explore the expressions of IL-1β and COX-2 in the gingiva tissues in rat periodontitis model with different estrogen levels, and the effect of estrogen level on periodontitis.Methods:A total of 40 female Wistar rats were randomized into 4 groups, i.e. normal control group (n=10), periodontitis group (n=10), castraction periodontitis group (n=10), and estrogen therapy group (n=10). RT-PCR was used to detect the expressions of IL-1β and COX-2 in the gingiva tissues in each group.Results:The expression intensity of IL-1β and COX-2 in the estrogen therapy group was significantly lower than that in the castraction periodontitis group (P<0.05).Conclusions:Estrogen can significantly down regulate the expressions of IL-1β and COX-2 in order to alleviate the symptoms of periodontitis.
