Prostate cancer risk and aggressiveness associated with the CYPIB1 4326C/G （Leu432Val） polymorphism： a meta-analysis of 2788 cases and 2968 controls

To derive a precise estimation of the associations between the cytochrome P450 1B 1 （CYPIB1） 4326C/G variants and prostate cancer （PCa） risk or aggressiveness, a meta-analysis was performed using all eligible published studies. Odds ratios （ORs） with 95% confidence intervals （CIs） were estimated to assess the association in seven literature studies with 2788 cases and 2968 controls. In the overall analysis, no significant association was found between the CYPIB1 4326C/G polymorphism and PCa risk, but ethnicity subgroup analyses and a case-source analysis revealed significant associations. The 4326G allele showed a significant association with increased PCa risk in Asians （OR= 1.52, 95% Ch 1.20-1.92）, and significant associations were also observed in a heterozygote comparison （OR= 1.40, 95% Ch 1.03-1.89）, a homozygote comparison （0R=2.38, 95% Ch 1.31-4.33） and in a dominant genetic model （OR = 1.52, 95% Ch 1.14-2.01）. Moreover, the 4326G allele was also significantly correlated with an increased risk of sporadic PCa （OR= 1.13, 95% Ch 1.04-1.24）, and significant associations were observed in a heterozygote comparison （OR= 1.16, 95% Ch 1.02-1.33）, a homozygote comparison （OR= 1.24, 95% Ch 1.03-1.49） and a dominant genetic model （OR= 1.19, 95% Ch 1.05- 1.34）. The overall analyses and all subgroup analyses showed no significant association between the 4326C/G polymorphism and PCa aggressiveness. Our meta-analysis showed that CYPIB1 4326G allele is significantly associated with an increased PCa risk in Asians and in sporadic PCa cases.

Aberrant DNA-PKcs and ERGIC1 expression may be involved in initiation of gastric cancer

AIM to investigate the molecular mechanisms of gastric carcinogenesis.METHODS We used label-free quantification technology integrated with liquid chromatography-tandem mass spectrometry(Lc-m S/m S) analysis to identify differentially expressed proteins in 160 specimens of normal gastric mucosa,gastric mucosa with mild dysplasia,moderate dysplasia,severe dysplasia,and early mucosal gastric cancer(Gc) collected at the Second Hospital of Lanzhou University from 2010 to 2015. Immunohistochemistry was used to verify the differentially expressed proteins detected by Lc-m S/m S.RESULTS With a threshold of a 1.2-fold change and a P-value< 0.05 between mild dysplasia,moderate dysplasia,severe dysplasia or early mucosal Gc and matched normal gastric mucosa tissues,proteomic analysis identified 365 significantly differentially expressed proteins. Er GIc1 expression decreased,while DNAPKcs expression increased gradually along with different stages of Gc initiation based on the tendency of fold change. the expression patterns of Er GIc1 and DNA-PKcs revealed by immunohistochemistry were consistent with the Lc-m S/m S results.CONCLUSION the results suggest that aberrant Er GIc1 and DNAPKcs expression may be involved in Gc initiation.