Objective To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV). Methods U251 cells were cultured in RPMI 1640 culture medium and ...Objective To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV). Methods U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD 169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV. Results The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P〈0.05). Conclusion HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.展开更多
A spectrum of neuropsychiatric disorders is a common complication from stroke.Neuropsychiatric disorders after stroke have negative effects on functional recovery,increasing the rate of mortality and disability of str...A spectrum of neuropsychiatric disorders is a common complication from stroke.Neuropsychiatric disorders after stroke have negative effects on functional recovery,increasing the rate of mortality and disability of stroke survivors.Given the vital significance of maintaining physical and mental health in stroke patients,neuropsychiatric issues after stroke have raised concerns by clinicians and researchers.This mini-review focuses on the most common non-cognitive functional neuropsychiatric disorders seen after stroke,including depressive disorders,anxiety disorders,post-traumatic stress disorder,psychosis,and psychotic disorders.For each condition,the clinical performance,epidemiology,identification of the therapeutic implication,and strategies are reviewed and discussed;the main opinions and perspectives presented here are based on the latest controlled studies,meta-analysis,or updated systematic reviews.In the absence of data from controlled studies,consensus recommendations were provided accordingly.展开更多
Human cytomegalovirus(HCMV) is the most common cause of congenital infection,resulting in birth defects such as microcephaly. In this study,RT-PCR and Western Blotting were performed to quantify the regulation of endo...Human cytomegalovirus(HCMV) is the most common cause of congenital infection,resulting in birth defects such as microcephaly. In this study,RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia cells by HCMV infection. The results showed that basal,endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells.展开更多
Objective:To discuss the effect of dimethyl fumarate(DMF) on rats with L-arginine induced chronic pancreatitis(CP).Methods:Male Wistar rats were given DMF treatment(25 mg/kg) by oral lavage method;then Wistar rats wer...Objective:To discuss the effect of dimethyl fumarate(DMF) on rats with L-arginine induced chronic pancreatitis(CP).Methods:Male Wistar rats were given DMF treatment(25 mg/kg) by oral lavage method;then Wistar rats were given the intraperitoneal injection of L-arginine for 5times(250 mg/100 kg,twice per time,each interval of 1 h) for building of CP model.Rats were divided into control group,CP group,DMF group and CP+DMF group.Rats in CP+DMF group were given the oral intragastric administration of DMF(25 mg/kg),while rats in control group and CP group were given the equal volume of normal saline.The weight of rats was evaluated and the intraperitoneal glucose tolerance test was performed(IPGTT,2 g/kg).The islet of rats was isolated and then flow cytometry was employed to evaluate the quality and activity of islets.Meanwhile,the histology of non-endocrine tissues and levels of myeloperoxidase(MPO) and malondialdchyde(MDA) were detected.Results:Compared with control group,the weight of rats in CP group was significantly reduced at week 2,4 and 6;the blood glucose significantly increased,AUC increased,the histopathological scores of pancreatic atrophy,acinar injury,edema and cellular infiltration increased,levels of MDA and MPO increased,the islet equivalent and islet activity decreased at 0.30,60,120 and 180 min.Compared with CP group,the weight of rats in CP+DMF group significantly increased at week 2,4 and 6;the blood glucose significantly decreased.AUC decreased,the histopathological scores of pancreatic atrophy,acinar injury,edema and cellular infiltration decreased,levels of MDA and MPO decreased,the islet equivalent and islet activity increased at 0,30.60,120 and 180 min.Conclusions:DMF treatment can improve CP induced by L-argininc and islet function in rats.展开更多
BACKGROUND Empyema is a severe complication following pneumonectomy that is associated with high morbidity and mortality rates.Although there are a wide variety of treatment options,successful management remains chall...BACKGROUND Empyema is a severe complication following pneumonectomy that is associated with high morbidity and mortality rates.Although there are a wide variety of treatment options,successful management remains challenging when this condition is combined with a large cavity in very thin patients who had previously undergone a posterolateral thoracotomy.CASE SUMMARY We reported the case of a thin,63-year-old man with a progressive pulmonary cyst who underwent left pneumonectomy via posterolateral thoracotomy 23 years ago.After an initially uneventful postoperative course,he was readmitted with empyema and a large cavity 21 years after surgery.He was successfully treated with limited thoracoplasty,followed by free vastus lateralis musculocutaneous flap transposition.CONCLUSION This case highlights that the treatment mode of limited thoracoplasty and free vastus lateralis musculocutaneous flap transposition is safe and effective for the management of postpneumonectomy empyema with a large cavity in thin patients who had previously undergone a posterolateral thoracotomy.展开更多
The different influences of the general grain boundary and the twin boundary on the cavity formation were investigated in copper irradiated by helium at room temperature. Large number of the cavities with sizes smalle...The different influences of the general grain boundary and the twin boundary on the cavity formation were investigated in copper irradiated by helium at room temperature. Large number of the cavities with sizes smaller than 5 nm formed after irradiation, which were almost homogenously distributed inside the grains. However, the distribution of the cavities varied near the general grain boundary and the twin boundary. Cavities-depleted zone was readily observed near the general grain boundaries, which was rarely observed near the twin boundaries. Meanwhile, decoration of dense cavities within the grain boundary plane was observed. The results suggested the limited influence of the twin boundaries as the point defect sinks compared with the general grain boundaries.展开更多
Endotracheal/endobronchial metastasis (EEM) generally originates from nonpulmonary malignancies such as cancers of the breast, colon, and kidney. Although the incidence of EEM has been reported to be between 2.0% an...Endotracheal/endobronchial metastasis (EEM) generally originates from nonpulmonary malignancies such as cancers of the breast, colon, and kidney. Although the incidence of EEM has been reported to be between 2.0% and 50.0%, cases diagnosed after radical resection of primary lung cancer are extremely rare. We hereby presented a patient of squamous cell lung carcinoma who was confirmed to have EEM 3 years alter his radical resection of primary tumor.展开更多
To the Editor:Asthma control is the focus of modern asthma management,while only 2% of Chinese patients are controlled.[1] A good patient-doctor relationship is one of the first steps in the successful management of...To the Editor:Asthma control is the focus of modern asthma management,while only 2% of Chinese patients are controlled.[1] A good patient-doctor relationship is one of the first steps in the successful management of asthma;patients can know more information about their condition and the processes of their health through talking thoroughly with doctors.Due to large numbers of outpatients,most physicians in China spend no more than 5 min with a patient in clinics,limiting the opportunity to give the appropriate treatment and discuss further knowledge about asthma with the patients.展开更多
A 9% Cr ferritic steel weld metal containing 1% Co, partially substituted for nickel, was prepared by submerged arc welding (SAW) processing. The microstructure and creep properties of the weld metal were investigated...A 9% Cr ferritic steel weld metal containing 1% Co, partially substituted for nickel, was prepared by submerged arc welding (SAW) processing. The microstructure and creep properties of the weld metal were investigated. The microstructure exhibited a fully tempered martensitic structure free of δ-ferrite. The creep properties of the obtained weld metal were inferior to those of the P92 base metal at 600 and 650 °C. The values of A and n for weld metal in the Norton power law constitution at 650 °C are 1.1×10?21 and 8.1, respectively.展开更多
Background:Although many causative genes of hereditary spastic paraplegia(HSP)have been uncovered in recent years,there are still approximately 50% of HSP patients without genetically diagnosis,especially in autosomal...Background:Although many causative genes of hereditary spastic paraplegia(HSP)have been uncovered in recent years,there are still approximately 50% of HSP patients without genetically diagnosis,especially in autosomal recessive(AR)HSP patients.Rare studies have been performed to determine the genetic spectrum and clinical profiles of recessive HSP patients in the Chinese population.Methods:In this study,we investigated 24 Chinese index AR/sporadic patients by targeted next-generation sequencing(NGS),Sanger sequencing and multiplex ligation-dependent probe amplification(MLPA).Further functional studies were performed to identify pathogenicity of those uncertain significance variants.Results:We identified 11 mutations in HSP related genes including 7 novel mutations,including two(p.V1979_L1980delinsX,p.F2343 fs)in SPG11,two(p.T55 M,p.S308 T)in AP5Z1,one(p.S242N)in ALDH18A1,one(p.D597fs)in GBA2,and one(p.Q486X)in ATP13A2 in 8 index patients and their family members.Mutations in ALDH18A1,AP5Z1,CAPN1 and ATP13A2 genes were firstly reported in the Chinese population.Furthermore,the clinical phenotypes of the patients carrying mutations were described in detail.The mutation(p.S242 N)in ALDH18A1 decreased enzyme activity of P5CS and mutations(p.T55 M,p.S308 T)in AP5Z1 induced lysosomal dysfunction.Conclusion:Our results expanded the genetic spectrum and clinical profiles of AR-HSP patients and further demonstrated the efficiency and reliability of targeted NGS diagnosing suspected HSP patients.展开更多
基金supported by the National Natural Science Foundation of China, (No. 30770105)Mt. Tai Scholar Construction Engineering Special Foundation of Shandong Province
文摘Objective To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV). Methods U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD 169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV. Results The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P〈0.05). Conclusion HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.
基金National Natural Science Foundation of China,No.81801712,No.81801710,No.81771271the Science and Technology Project Funds from Education Department of Liaoning Province of China,No.LK2016022.
文摘A spectrum of neuropsychiatric disorders is a common complication from stroke.Neuropsychiatric disorders after stroke have negative effects on functional recovery,increasing the rate of mortality and disability of stroke survivors.Given the vital significance of maintaining physical and mental health in stroke patients,neuropsychiatric issues after stroke have raised concerns by clinicians and researchers.This mini-review focuses on the most common non-cognitive functional neuropsychiatric disorders seen after stroke,including depressive disorders,anxiety disorders,post-traumatic stress disorder,psychosis,and psychotic disorders.For each condition,the clinical performance,epidemiology,identification of the therapeutic implication,and strategies are reviewed and discussed;the main opinions and perspectives presented here are based on the latest controlled studies,meta-analysis,or updated systematic reviews.In the absence of data from controlled studies,consensus recommendations were provided accordingly.
基金National Natural Science Foundation of China (30770105)Mt. Tai Scholar Construction Engineering Special Foundation of Shandong province.
文摘Human cytomegalovirus(HCMV) is the most common cause of congenital infection,resulting in birth defects such as microcephaly. In this study,RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia cells by HCMV infection. The results showed that basal,endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells.
基金supported by the College Science Technology Research Project of Hebei Province(Grant No:ZD20131002)
文摘Objective:To discuss the effect of dimethyl fumarate(DMF) on rats with L-arginine induced chronic pancreatitis(CP).Methods:Male Wistar rats were given DMF treatment(25 mg/kg) by oral lavage method;then Wistar rats were given the intraperitoneal injection of L-arginine for 5times(250 mg/100 kg,twice per time,each interval of 1 h) for building of CP model.Rats were divided into control group,CP group,DMF group and CP+DMF group.Rats in CP+DMF group were given the oral intragastric administration of DMF(25 mg/kg),while rats in control group and CP group were given the equal volume of normal saline.The weight of rats was evaluated and the intraperitoneal glucose tolerance test was performed(IPGTT,2 g/kg).The islet of rats was isolated and then flow cytometry was employed to evaluate the quality and activity of islets.Meanwhile,the histology of non-endocrine tissues and levels of myeloperoxidase(MPO) and malondialdchyde(MDA) were detected.Results:Compared with control group,the weight of rats in CP group was significantly reduced at week 2,4 and 6;the blood glucose significantly increased,AUC increased,the histopathological scores of pancreatic atrophy,acinar injury,edema and cellular infiltration increased,levels of MDA and MPO increased,the islet equivalent and islet activity decreased at 0.30,60,120 and 180 min.Compared with CP group,the weight of rats in CP+DMF group significantly increased at week 2,4 and 6;the blood glucose significantly decreased.AUC decreased,the histopathological scores of pancreatic atrophy,acinar injury,edema and cellular infiltration decreased,levels of MDA and MPO decreased,the islet equivalent and islet activity increased at 0,30.60,120 and 180 min.Conclusions:DMF treatment can improve CP induced by L-argininc and islet function in rats.
文摘BACKGROUND Empyema is a severe complication following pneumonectomy that is associated with high morbidity and mortality rates.Although there are a wide variety of treatment options,successful management remains challenging when this condition is combined with a large cavity in very thin patients who had previously undergone a posterolateral thoracotomy.CASE SUMMARY We reported the case of a thin,63-year-old man with a progressive pulmonary cyst who underwent left pneumonectomy via posterolateral thoracotomy 23 years ago.After an initially uneventful postoperative course,he was readmitted with empyema and a large cavity 21 years after surgery.He was successfully treated with limited thoracoplasty,followed by free vastus lateralis musculocutaneous flap transposition.CONCLUSION This case highlights that the treatment mode of limited thoracoplasty and free vastus lateralis musculocutaneous flap transposition is safe and effective for the management of postpneumonectomy empyema with a large cavity in thin patients who had previously undergone a posterolateral thoracotomy.
基金supported by the National Natural Science Foundation of China(No.11275023)the National Magnetic Confinement Fusion Program(No.2011GB110004)
文摘The different influences of the general grain boundary and the twin boundary on the cavity formation were investigated in copper irradiated by helium at room temperature. Large number of the cavities with sizes smaller than 5 nm formed after irradiation, which were almost homogenously distributed inside the grains. However, the distribution of the cavities varied near the general grain boundary and the twin boundary. Cavities-depleted zone was readily observed near the general grain boundaries, which was rarely observed near the twin boundaries. Meanwhile, decoration of dense cavities within the grain boundary plane was observed. The results suggested the limited influence of the twin boundaries as the point defect sinks compared with the general grain boundaries.
文摘Endotracheal/endobronchial metastasis (EEM) generally originates from nonpulmonary malignancies such as cancers of the breast, colon, and kidney. Although the incidence of EEM has been reported to be between 2.0% and 50.0%, cases diagnosed after radical resection of primary lung cancer are extremely rare. We hereby presented a patient of squamous cell lung carcinoma who was confirmed to have EEM 3 years alter his radical resection of primary tumor.
文摘To the Editor:Asthma control is the focus of modern asthma management,while only 2% of Chinese patients are controlled.[1] A good patient-doctor relationship is one of the first steps in the successful management of asthma;patients can know more information about their condition and the processes of their health through talking thoroughly with doctors.Due to large numbers of outpatients,most physicians in China spend no more than 5 min with a patient in clinics,limiting the opportunity to give the appropriate treatment and discuss further knowledge about asthma with the patients.
基金Project (No 51074113) supported by the National Natural ScienceFoundation of China
文摘A 9% Cr ferritic steel weld metal containing 1% Co, partially substituted for nickel, was prepared by submerged arc welding (SAW) processing. The microstructure and creep properties of the weld metal were investigated. The microstructure exhibited a fully tempered martensitic structure free of δ-ferrite. The creep properties of the obtained weld metal were inferior to those of the P92 base metal at 600 and 650 °C. The values of A and n for weld metal in the Norton power law constitution at 650 °C are 1.1×10?21 and 8.1, respectively.
基金This study was supported by a grant from the National Natural Science Foundation of China to Zhi-Ying Wu(81125009)the research foundation for distinguished scholar of Zhejiang University to Zhi-Ying Wu(188020-193810101/089)the Fundamental Research Funds for the Central Universities(2019XZZX001-01-04).
文摘Background:Although many causative genes of hereditary spastic paraplegia(HSP)have been uncovered in recent years,there are still approximately 50% of HSP patients without genetically diagnosis,especially in autosomal recessive(AR)HSP patients.Rare studies have been performed to determine the genetic spectrum and clinical profiles of recessive HSP patients in the Chinese population.Methods:In this study,we investigated 24 Chinese index AR/sporadic patients by targeted next-generation sequencing(NGS),Sanger sequencing and multiplex ligation-dependent probe amplification(MLPA).Further functional studies were performed to identify pathogenicity of those uncertain significance variants.Results:We identified 11 mutations in HSP related genes including 7 novel mutations,including two(p.V1979_L1980delinsX,p.F2343 fs)in SPG11,two(p.T55 M,p.S308 T)in AP5Z1,one(p.S242N)in ALDH18A1,one(p.D597fs)in GBA2,and one(p.Q486X)in ATP13A2 in 8 index patients and their family members.Mutations in ALDH18A1,AP5Z1,CAPN1 and ATP13A2 genes were firstly reported in the Chinese population.Furthermore,the clinical phenotypes of the patients carrying mutations were described in detail.The mutation(p.S242 N)in ALDH18A1 decreased enzyme activity of P5CS and mutations(p.T55 M,p.S308 T)in AP5Z1 induced lysosomal dysfunction.Conclusion:Our results expanded the genetic spectrum and clinical profiles of AR-HSP patients and further demonstrated the efficiency and reliability of targeted NGS diagnosing suspected HSP patients.