Chronic heart failure(CHF)is a public health problem that seriously affects the quality of life of patients with poor prognosis.Western medicine has been mature in the treatment of CHF,but it still has some limitation...Chronic heart failure(CHF)is a public health problem that seriously affects the quality of life of patients with poor prognosis.Western medicine has been mature in the treatment of CHF,but it still has some limitations and adverse reactions in improving the clinical symptoms of CHF.TCM has a long history of understanding of CHF,and in recent years,TCM has summarized and developed the experience of predecessors in the treatment of CHF,In particular,diuretic therapy,combined with Warming Yang,tonifying Qi,promoting blood circulation and removing blood stasis,has achieved good curative effect in treating CHF.When combined with basic treatment of Western medicine,it can reduce the adverse reactions of Western medicine.This review is to summarize.展开更多
Objective: To study the correlation of TGF-β and IGF-1 signaling pathway expression in nephroblastoma tissue with cell proliferation activity and invasion activity. Methods:Nephroblastoma tumor tissue and adjacent ti...Objective: To study the correlation of TGF-β and IGF-1 signaling pathway expression in nephroblastoma tissue with cell proliferation activity and invasion activity. Methods:Nephroblastoma tumor tissue and adjacent tissue surgically removed in Renmin Hospital of Wuhan University between May 2014 and March 2017 were selected, the expression levels of TGF-β and IGF-1 signal pathway molecules were determined after protein extraction, and the mRNA expression levels of cell proliferation genes and invasion genes were determined after RNA extraction. Results: TGFBR1, IGF1R, p-ERK, Smad2/3 and p-AKT protein expression levels in nephroblastoma tumor tissue were significantly higher than those in adjacent tissue;DNp73, Survivin, Ki-67, TUBB3, MMP2 and N-cadherin mRNA expression levels in nephroblastoma tumor tissue were significantly higher than those in adjacent tissue and positively correlated with TGFBR1, IGF1R, p-ERK, Smad2/3 and p-AKT protein expression levels while Bmi, TAp73, TIMP1, TIMP2 and E-cadherin mRNA expression levels were significantly lower than those in adjacent tissue and negatively correlated with TGFBR1, IGF1R, p-ERK, Smad2/3 and p-AKT protein expression levels. Conclusion: The high expression of TGF-β and IGF-1 signaling pathways in nephroblastoma tissue can promote the proliferation and invasion of tumor cells.展开更多
基金National Natural Science Foundation of China(No.81673891,81560760)Project of Health Department of Guangxi Zhuang Autonomous Region(No.GZZJ16-03)+1 种基金Project of Health and Family Planning Commission of Guangxi Zhuang Autonomous Region(No.S201532)Second Batch of Special Scientific Research Projects for the Construction of the National TCM Clinical Research Base(No.JDZX2015146)。
文摘Chronic heart failure(CHF)is a public health problem that seriously affects the quality of life of patients with poor prognosis.Western medicine has been mature in the treatment of CHF,but it still has some limitations and adverse reactions in improving the clinical symptoms of CHF.TCM has a long history of understanding of CHF,and in recent years,TCM has summarized and developed the experience of predecessors in the treatment of CHF,In particular,diuretic therapy,combined with Warming Yang,tonifying Qi,promoting blood circulation and removing blood stasis,has achieved good curative effect in treating CHF.When combined with basic treatment of Western medicine,it can reduce the adverse reactions of Western medicine.This review is to summarize.
基金Hubei Provincial Natural Science Foundation Project No:2013CFB244.
文摘Objective: To study the correlation of TGF-β and IGF-1 signaling pathway expression in nephroblastoma tissue with cell proliferation activity and invasion activity. Methods:Nephroblastoma tumor tissue and adjacent tissue surgically removed in Renmin Hospital of Wuhan University between May 2014 and March 2017 were selected, the expression levels of TGF-β and IGF-1 signal pathway molecules were determined after protein extraction, and the mRNA expression levels of cell proliferation genes and invasion genes were determined after RNA extraction. Results: TGFBR1, IGF1R, p-ERK, Smad2/3 and p-AKT protein expression levels in nephroblastoma tumor tissue were significantly higher than those in adjacent tissue;DNp73, Survivin, Ki-67, TUBB3, MMP2 and N-cadherin mRNA expression levels in nephroblastoma tumor tissue were significantly higher than those in adjacent tissue and positively correlated with TGFBR1, IGF1R, p-ERK, Smad2/3 and p-AKT protein expression levels while Bmi, TAp73, TIMP1, TIMP2 and E-cadherin mRNA expression levels were significantly lower than those in adjacent tissue and negatively correlated with TGFBR1, IGF1R, p-ERK, Smad2/3 and p-AKT protein expression levels. Conclusion: The high expression of TGF-β and IGF-1 signaling pathways in nephroblastoma tissue can promote the proliferation and invasion of tumor cells.
