Circular RNA hsacirc0000745 may serve as a diagnostic marker for gastric cancer

AIM To determine whether circular RNAs(circ RNAs) are involved in pathological processes of gastric cancer(GC).METHODS Three circ RNAs with differential expression in GC and colorectal cancer were randomly selected for validation by quantitative reverse transcription-polymerase chain reaction(q RT-PCR), using 20 pairs of gastric tissues and normal tissues. Based on the predicted circ RNAmi RNA network, we then focused on hsa_circ_0000745, which was found to be down-regulated in 20 GC tissues compared with normal tissues. The hsa_circ_0000745 levels were further analyzed by q RT-PCR in 60 GC tissues and paired adjacent non-tumor tissues, as well as 60 plasma samples from GC patients and 60 plasma samples from healthy controls. The associations between the levels of hsa_circ_0000745 and the clinicopathological features of GC patients were statistically assessed. A receiver operating characteristic(ROC) curve was used to evaluate the diagnostic value of hsa_circ_0000745 in GC.RESULTS Hsa_circ_0000745 was down-regulated in GC tissues vs non-tumorous tissues(P < 0.001) and in plasma samples from patients with GC vs healthy controls(P < 0.001). The expression level of hsa_circ_0000745 in GC tissues correlated with tumor differentiation, while the expression level in plasma correlated with tumor-nodemetastasis stage. The area under the ROC curve(AUC) of hsa_circ_0000745 in plasma was 0.683, suggesting good diagnostic value. Plasma hsa_circ_0000745 level combined with carcinoembryogenic antigen(CEA) level increased the AUC to 0.775.CONCLUSION Hsa_circ_0000745 plays an important role in GC and its expression level in plasma in combination with CEA level is a promising diagnostic marker for this malignancy.

Experiment and analysis on flow rate and temperature of liquid PbBi in PREKY

Flow rate and temperature are important parameters for design and operation of liquid lead–bismuth(Pb Bi) experimental loop. The PREKY facility was designed to study test technique of flow rate and local temperature of Pb Bi loop. In this work, flow rate monitoring of molten Pb Bi was performed, and temperatures of the Pb Bi and pipe surface were measured. The results show that the flow rate of the venturi-nozzle flow meter had an uncertainty of ±5 % in the range of 0.6–2.0 m/s, and the maximum temperature difference between the Pb Bi and pipe surface was about 8 ℃.

Variation in copper and zinc tolerance and accumulation in 12 willow clones： implications for phytoextraction

研究目的:柳树(Salix spp.)已广泛用于修复重金属污染,而且修复效率与树种及无性系有关。目前涉及柳树研究主要为铜(Cu)和锌(Zn)的毒性效应,而不是富集能力。本研究以中国常见的柳树无性系评价对Cu和Zn耐性与富集能力。创新要点:目前大多数研究仍集中Cu和Zn对柳树的毒性效应,尤其是Cu,对它们富集能力仍没有评价。中国为柳树的主要分布区。本文以12种优良的常见柳树无性系,通过温室营养液法评价对Cu和Zn耐性及富集潜力差异。利用柳树无性系的耐性与富集变异,旨在选择高耐性与高富集的无性系,提高环境修复效率和加快修复进程。研究方法:选择高生物量12种柳树无性系,采用温室营养液培养法添加Cu或Zn处理,测定生物量参数并计算耐性指数,估计耐性差异;用原子吸收法测定组织中金属浓度并计算金属位移系数,评价富集差异。重要结论:无性系对Cu和Zn耐性显著不同,生物量变化从无影响、抑制到促进生长。无性系对Cu耐性高于对Zn耐性,无性系对Zn富集变异幅度大于对Cu富集变异幅度。Cu主要富集于根部,而Zn较易运输到地上部分。总之测定的大部分柳树无性系为Cu与Zn高富集植物,显示出较高的植物提取能力。