Nucleophosmin/B23 (NPM) is a universally expressed nucleolar phosphoprotein that participates in proliferation, apoptosis, ribosome assembly, and centrosome duplication; however, the role of NPM in cell cycle regulati...Nucleophosmin/B23 (NPM) is a universally expressed nucleolar phosphoprotein that participates in proliferation, apoptosis, ribosome assembly, and centrosome duplication; however, the role of NPM in cell cycle regulation is not well characterized. We investigated the mechanism by which NPM is involved in cell cycle regulation. NPM was knocked down using siRNA in HepG2 hepatoblastoma cells. NPM translocation following actinomycin D (ActD) treatment was investigated using immunofluorescent staining. Expression of NPM and other factors involved in cell cycle regulation was examined by Western blotting. Cell cycle distribution was measured using flow cytometry to detect 5-ethynyl-2′-deoxyuridine (EdU) incorporation. Cell proliferation was quantified by the MTT assay. Knockdown of NPM increased the percentage of HepG2 cells in S phase and led to decreased expression of P53 and P21Cip1/WAF1. S-phase arrest in HepG2 cells was significantly enhanced by ActD treatment. Furthermore, knockdown of NPM abrogated ActD-induced G2/M phase cell cycle arrest. Taken together, these data demonstrate that inhibition of NPM has a significant effect on the cell cycle.展开更多
Background： Population-based cancer registry collects the data on cancer incidence and mortality deaths from covered population to describe and survey the epidemics in certain areas. The aim of this study was to esti...Background： Population-based cancer registry collects the data on cancer incidence and mortality deaths from covered population to describe and survey the epidemics in certain areas. The aim of this study was to estimate the cancer incidence and mortality in Wuwei, Gansu province, Northwestern China from 2003 to 2012. The goal is to better understand cancer distribution and long-term development of cancer prevention and treatment in Wuwei. Methods： Data were collected from the Wuwei Cancer Registry between 2003 and 2012. In this registry, data from 46 cancer report centers were included in this analysis. Incidence/mortality rates, age-specific incidence/mortality rates, age-standardized incidence/mortality rates, and cumulative incidence/mortality rates were calculated. Totally, 9,836,740 person-years （5,110,342 for males and 4,726,398 for females） had been monitored over this time period. The gender ratio of male/female was 1.08：1. The number of new cancer cases and related deaths was 24,705 and 17,287 from 2003 to 2012, respectively. Results： The proportion of morphological verification was 74.43%. The incidence of cases identified through death certification only was 1.21%, and the mortality to incidence ratio was 0.70. The average crude incidence was 251.15/100,000 persons （310.61 and 186.87 for males and females per 100,000 persons, respectively）. The age-standardized rates by Chinese standard population （ASR-China） and by world standard population （ASR-world） were 207.76 and 245.42 per 100,000 persons, respectively. The crude cancer mortality was 175.74/100,000 persons （228.34 and 118.86 for males and females per 100,000 persons）. ASR for China and the world was 149.57 and 175.13/100,000 persons, respectively. The most common cancers and leading causes of cancer-related deaths in Wuwei were as follows： cancers of stomach, esophagus, liver, lung, colorectum, breast, cervix, lymphoma, blood （leukemia）, brain, and central nervous system. In Wuwei, during 2003 and 2012, cancer incidence and mortality rates increased by 1.32% and 1.3 l%/year, respectively. During this time, colorectum cancer incidence and mortality rates increased by 2.69% and 7.54%/year, respectively, in Wuwei. The incidence and mortality of other gastric, esophageal, liver, and lung cancers also all increased. Conclusions： The results of this study report a more accurate cancer burden among the population of Wuwei, China. Active research of cancers etiology and effective prevention should be established to reduce the incidence and mortality associated with cancers.展开更多
Osteoarthritis(OA)is currently considered as more than a degenerative disorder of weight-bearing joints,instead one organ disease of“all-joint”due to cause(s)of aging,injury,inflammation,metabolic disorders,etc.Its ...Osteoarthritis(OA)is currently considered as more than a degenerative disorder of weight-bearing joints,instead one organ disease of“all-joint”due to cause(s)of aging,injury,inflammation,metabolic disorders,etc.Its prevalence is high:9.6%in men and 18.0%in women aged over 60 years.Most health professionals say that OA is incurable and focus exclusively on palliative treatment,as we lack effective disease modifying osteoarthritis drugs(DMOADs).[1]But should we hope that treating OA precisely in China is possible through intensive research and drug development?展开更多
基金supported by grants from theNational Natural Science Foundation of China (No.81172345 and No. 30973506)the National High Technology Research and Development Program of China (863 Program) (2006AA02A404 to Y-X.Z.)
文摘Nucleophosmin/B23 (NPM) is a universally expressed nucleolar phosphoprotein that participates in proliferation, apoptosis, ribosome assembly, and centrosome duplication; however, the role of NPM in cell cycle regulation is not well characterized. We investigated the mechanism by which NPM is involved in cell cycle regulation. NPM was knocked down using siRNA in HepG2 hepatoblastoma cells. NPM translocation following actinomycin D (ActD) treatment was investigated using immunofluorescent staining. Expression of NPM and other factors involved in cell cycle regulation was examined by Western blotting. Cell cycle distribution was measured using flow cytometry to detect 5-ethynyl-2′-deoxyuridine (EdU) incorporation. Cell proliferation was quantified by the MTT assay. Knockdown of NPM increased the percentage of HepG2 cells in S phase and led to decreased expression of P53 and P21Cip1/WAF1. S-phase arrest in HepG2 cells was significantly enhanced by ActD treatment. Furthermore, knockdown of NPM abrogated ActD-induced G2/M phase cell cycle arrest. Taken together, these data demonstrate that inhibition of NPM has a significant effect on the cell cycle.
文摘Background： Population-based cancer registry collects the data on cancer incidence and mortality deaths from covered population to describe and survey the epidemics in certain areas. The aim of this study was to estimate the cancer incidence and mortality in Wuwei, Gansu province, Northwestern China from 2003 to 2012. The goal is to better understand cancer distribution and long-term development of cancer prevention and treatment in Wuwei. Methods： Data were collected from the Wuwei Cancer Registry between 2003 and 2012. In this registry, data from 46 cancer report centers were included in this analysis. Incidence/mortality rates, age-specific incidence/mortality rates, age-standardized incidence/mortality rates, and cumulative incidence/mortality rates were calculated. Totally, 9,836,740 person-years （5,110,342 for males and 4,726,398 for females） had been monitored over this time period. The gender ratio of male/female was 1.08：1. The number of new cancer cases and related deaths was 24,705 and 17,287 from 2003 to 2012, respectively. Results： The proportion of morphological verification was 74.43%. The incidence of cases identified through death certification only was 1.21%, and the mortality to incidence ratio was 0.70. The average crude incidence was 251.15/100,000 persons （310.61 and 186.87 for males and females per 100,000 persons, respectively）. The age-standardized rates by Chinese standard population （ASR-China） and by world standard population （ASR-world） were 207.76 and 245.42 per 100,000 persons, respectively. The crude cancer mortality was 175.74/100,000 persons （228.34 and 118.86 for males and females per 100,000 persons）. ASR for China and the world was 149.57 and 175.13/100,000 persons, respectively. The most common cancers and leading causes of cancer-related deaths in Wuwei were as follows： cancers of stomach, esophagus, liver, lung, colorectum, breast, cervix, lymphoma, blood （leukemia）, brain, and central nervous system. In Wuwei, during 2003 and 2012, cancer incidence and mortality rates increased by 1.32% and 1.3 l%/year, respectively. During this time, colorectum cancer incidence and mortality rates increased by 2.69% and 7.54%/year, respectively, in Wuwei. The incidence and mortality of other gastric, esophageal, liver, and lung cancers also all increased. Conclusions： The results of this study report a more accurate cancer burden among the population of Wuwei, China. Active research of cancers etiology and effective prevention should be established to reduce the incidence and mortality associated with cancers.
基金The study was supported by grants from the National Natural Science Foundation of China(No.81771748)the Shenzhen Science and Technology Project(No.JCYJ20180504170414637)the Sanming Project of Medicine in Shenzhen(No.SZSM201602087)。
文摘Osteoarthritis(OA)is currently considered as more than a degenerative disorder of weight-bearing joints,instead one organ disease of“all-joint”due to cause(s)of aging,injury,inflammation,metabolic disorders,etc.Its prevalence is high:9.6%in men and 18.0%in women aged over 60 years.Most health professionals say that OA is incurable and focus exclusively on palliative treatment,as we lack effective disease modifying osteoarthritis drugs(DMOADs).[1]But should we hope that treating OA precisely in China is possible through intensive research and drug development?
