Influence of spinal cord injury on core regions of motor function

Functional electrical stimulation is an effective way to rebuild hindlimb motor function after spinal cord injury.However,no site map exists to serve as a reference for implanting stimulator electrodes.In this study,rat models of thoracic spinal nerve 9 contusion were established by a heavy-impact method and rat models of T6/8/9 spinal cord injury were established by a transection method.Intraspinal microstimulation was performed to record motion types,site coordinates,and threshold currents induced by stimulation.After transection(complete injury),the core region of hip flexion migrated from the T13 to T12 vertebral segment,and the core region of hip extension migrated from the L1 to T13 vertebral segment.Migration was affected by post-transection time,but not transection segment.Moreover,the longer the post-transection time,the longer the distance of migration.This study provides a reference for spinal electrode implantation after spinal cord injury.This study was approved by the Institutional Animal Care and Use Committee of Nantong University,China(approval No.20190225-008)on February 26,2019.

Real-time and wearable functional electrical stimulation system for volitional hand motor function control using the electromyography bridge method

Voluntary participation of hemiplegic patients is crucial for functional electrical stimulation therapy.A wearable functional electrical stimulation system has been proposed for real-time volitional hand motor function control using the electromyography bridge method.Through a series of novel design concepts,including the integration of a detecting circuit and an analog-to-digital converter,a miniaturized functional electrical stimulation circuit technique,a low-power super-regeneration chip for wireless receiving,and two wearable armbands,a prototype system has been established with reduced size,power,and overall cost.Based on wrist joint torque reproduction and classification experiments performed on six healthy subjects,the optimized surface electromyography thresholds and trained logistic regression classifier parameters were statistically chosen to establish wrist and hand motion control with high accuracy.Test results showed that wrist flexion/extension,hand grasp,and finger extension could be reproduced with high accuracy and low latency.This system can build a bridge of information transmission between healthy limbs and paralyzed limbs,effectively improve voluntary participation of hemiplegic patients,and elevate efficiency of rehabilitation training.

Quantitative evaluation of extrinsic factors influencing electrical excitability in neuronal networks： Voltage Threshold Measurement Method(VTMM)

The electrical excitability of neural networks is influenced by different environmental factors. Effective and simple methods are required to objectively and quantitatively evaluate the influence of such factors, including variations in temperature and pharmaceutical dosage. The aim of this paper was to introduce ‘the voltage threshold measurement method＇, which is a new method using microelectrode arrays that can quantitatively evaluate the influence of different factors on the electrical excitability of neural networks. We sought to verify the feasibility and efficacy of the method by studying the effects of acetylcholine, ethanol, and temperature on hippocampal neuronal networks and hippocampal brain slices. First, we determined the voltage of the stimulation pulse signal that elicited action potentials in the two types of neural networks under normal conditions. Second, we obtained the voltage thresholds for the two types of neural networks under different concentrations of acetylcholine, ethanol, and different temperatures. Finally, we obtained the relationship between voltage threshold and the three influential factors. Our results indicated that the normal voltage thresholds of the hippocampal neuronal network and hippocampal slice preparation were 56 and 31 m V, respectively. The voltage thresholds of the two types of neural networks were inversely proportional to acetylcholine concentration, and had an exponential dependency on ethanol concentration. The curves of the voltage threshold and the temperature of the medium for the two types of neural networks were U-shaped. The hippocampal neuronal network and hippocampal slice preparations lost their excitability when the temperature of the medium decreased below 34 and 33°C or increased above 42 and 43°C, respectively. These results demonstrate that the voltage threshold measurement method is effective and simple for examining the performance/excitability of neuronal networks.

A flexible electrode array for determining regions of motor function activated by epidural spinal cord stimulation in rats with spinal cord injury

Epidural stimulation of the spinal cord is a promising technique for the recovery of motor function after spinal cord injury.The key challenges within the reconstruction of motor function for paralyzed limbs are the precise control of sites and parameters of stimulation.To activate lower-limb muscles precisely by epidural spinal cord stimulation,we proposed a high-density,flexible electrode array.We determined the regions of motor function that were activated upon epidural stimulation of the spinal cord in a rat model with complete spinal cord,which was established by a transection method.For evaluating the effect of stimulation,the evoked potentials were recorded from bilateral lowerlimb muscles,including the vastus lateralis,semitendinosus,tibialis anterior,and medial gastrocnemius.To determine the appropriate stimulation sites and parameters of the lower muscles,the stimulation characteristics were studied within the regions in which motor function was activated upon spinal cord stimulation.In the vastus lateralis and medial gastrocnemius,these regions were symmetrically located at the lateral site of L1 and the medial site of L2 vertebrae segment,respectively.The tibialis anterior and semitendinosus only responded to stimulation simultaneously with other muscles.The minimum and maximum stimulation threshold currents of the vastus lateralis were higher than those of the medial gastrocnemius.Our results demonstrate the ability to identify specific stimulation sites of lower muscles using a high-density and flexible array.They also provide a reference for selecting the appropriate conditions for implantable stimulation for animal models of spinal cord injury.This study was approved by the Animal Research Committee of Southeast University,China(approval No.20190720001) on July 20,2019.

Ka-band ultra low voltage miniature sub-harmonic resistive mixer with a new broadside coupled Marchand balun in 0.18-μm CMOS technology

A Ka-band sub-harmonically pumped resistive mixer(SHPRM) was designed and fabricated using the standard 0.18-μm complementary metal-oxide-semiconductor(CMOS) technology.An area-effective asymmetric broadside coupled spiral Marchand balance-to-unbalance(balun) with magnitude and phase imbalance compensation is used in the mixer to transform local oscillation(LO) signal from single to differential mode.The results showed that the SHPRM achieves the conversion gain of-15--12.5 dB at fixed fIF=0.5 GHz with 8 dBm LO input power for the radio frequency(RF) bandwidth of 28-35 GHz.The in-band LO-intermediate freqency(IF),RF-IF,and LO-RF isolations are better than 31,34,and 36 dB,respectively.Besides,the 2LO-IF and 2LO-RF isolations are better than 60 and 45 dB,respectively.The measured input referred P1dB and 3rd-order inter-modulation intercept point(IIP3) are 0.5 and 10.5 dBm,respectively.The measurement is performed under a gate bias voltage as low as 0.1 V and the whole chip only occupies an area of 0.33 mm2 including pads.

Folded down-conversion mixer for a 60 GHz receiver architecture in 65-nm CMOS technology

We present the design of a folded down-conversion mixer which is incorporated at the final down-conversion stage of a 60 GHz receiver. The mixer employs an ac-coupled current reuse transconductance stage. It performs well under low supply voltages, and is less sensitive to temperature variations and process spread. The mixer operates at an input radio frequency(RF) band ranging from 10.25 to 13.75 GHz, with a fixed local oscillator(LO) frequency of 12 GHz, which down-converts the RF band to an intermediate frequency(IF) band ranging from dc to 1.75 GHz. The mixer is designed in a 65 nm low power(LP) CMOS process with an active chip area of only 0.0179 mm2. At a nominal supply voltage of 1.2 V and an IF of 10 MHz, a maximum voltage conversion gain(VCG) of 9.8 d B, a double sideband noise figure(DSB-NF) of 11.6 d B, and a linearity in terms of input 1 d B compression point(Pin,1d B) of-13 d Bm are measured. The mixer draws a current of 5 m A from a 1.2 V supply dissipating a power of only 6 m W.

A 37 GHz wide-band programmable divide-by-N frequency divider for millimeter-wave silicon-based phase-locked loop frequency synthesizers

A 37 GHz wide-band programmable divide-by-N frequency divider(FD) composed of a divide-by-2 divider(acting as the first stage) and a divider with a division ratio range of 273–330(acting as the second stage) has been designed and fabricated using standard 90 nm CMOS technology. The second stage divider consists of a high-speed divide-by-8/9 dual-modulus prescaler, a pulse counter, and a swallow counter. Both the first stage divider(with high speed) and the divide-by-8/9 prescaler employ dynamic current-mode logic(DCML) structure to improve the operating performance. The first stage divider can work from 2 to 40 GHz and the whole divider covers a wide frequency range from 25 to 37 GHz. The input sensitivity is as low as-20 d Bm at 32 GHz and the phase noise at 37 GHz is less than-130 d Bc/Hz at an offset of 1 MHz. The whole chip dissipates 17.88 m W at a supply voltage of 1.2 V and occupies an area of only 730 μm×475 μm.

A 31–45.5 GHz injection-locked frequency divider in 90-nm CMOS technology

We present a 31–45.5 GHz injection-locked frequency divider(ILFD) implemented in a standard 90-nm CMOS process. To reduce parasitic capacitance and increase the operating frequency, an NMOS-only cross-coupled pair is adopted to provide negative resistance. Acting as an adjustable resistor, an NMOS transistor with a tunable gate bias voltage is connected to the differential output terminals for locking range extension. Measurements show that the designed ILFD can be fully functional in a wide locking range and provides a good figure-of-merit. Under a 1 V tunable bias voltage, the self-resonant frequency of the divider is 19.11 GHz and the maximum locking range is 37.7% at 38.5 GHz with an input power of 0 d Bm. The power consumption is 2.88 m W under a supply voltage of 1.2 V. The size of the chip including the pads is 0.62 mm×0.42 mm.