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Cellular automaton simulation of dynamic recrystallization behavior in V-10Cr-5Ti alloy under hot deformation conditions 被引量:5
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作者 Zhuo-han CAO Yu SUN +4 位作者 Chen ZHOU zhi-peng wan Wen-hua YANG Li-li REN Lian-xi HU 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2019年第1期98-111,共14页
The deformation behavior of V-10Cr-5Ti alloy was studied on the Gleeble-1500 thermomechanical simulator at the temperatures of 950-1350℃, and the strain rates of 0.01-10 s^-1. Based on the Arrhenius model, dislocatio... The deformation behavior of V-10Cr-5Ti alloy was studied on the Gleeble-1500 thermomechanical simulator at the temperatures of 950-1350℃, and the strain rates of 0.01-10 s^-1. Based on the Arrhenius model, dislocation density model, nucleation model and grain growth model, a numerical cellular automaton (CA) model coupling simulation of hot deformation is established to simulate and characterize the microstructural evolution during DRX. The results show that the flow stress is fairly sensitive to the strain rate and deformation temperature. The error between the predicted stress by the Arrhenius model and the actual measured value is less than 8%. The initial average grain size calculated by the CA model is 86.25 μm, which is close to the experimental result (85.63 μm). The simulations show that the effect of initial grain size on the dynamic recrystallization microstructure evolution is not significant, while increasing the strain rate or reducing the temperature can refine the recrystallized grains. 展开更多
关键词 V-10Cr-5Ti alloy hot deformation dynamic recrystallization cellular automaton MICROSTRUCTURE numerical simulation grain refinement
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Establishment of a novel constitutive model considering dynamic recrystallization behavior of Ti-22Al-25Nb alloy during hot deformation 被引量:7
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作者 Yu SUN Heng ZHANG +2 位作者 zhi-peng wan Li-li REN Lian-xi HU 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2019年第3期546-557,共12页
The hot deformation behavior of Ti-22Al-25Nb alloy fabricated by hot compressed sintering was investigated under various conditions of compression tests in the deformation temperature range of 975-1075 °C with 20... The hot deformation behavior of Ti-22Al-25Nb alloy fabricated by hot compressed sintering was investigated under various conditions of compression tests in the deformation temperature range of 975-1075 °C with 20 °C intervals and the strain rate range of 0.001-1.0 s^-1. Based on the experimental data, a novel constitutive relation combining a series of models was developed, including Zener-Hollomon parameter (Z), DRX critical model and kinetics model. The results show that the hot-deformed activation energy Q is calculated to be 410.172 kJ/mol, the ratio of critical strain (εc) to peak strain (εp) is a constant value of about 0.67. The predicted stress obtained by the established constitutive equations matches well with the true stress from experimental data. Despite large errors occur at the stage where strain rate is 0.1 s^-1 and the values of true strain are less than 0.1, the stage of large strain should be more concerned during plastic forming. Furthermore, the predicting accuracy with the DRX kinetics model was testified by an electron back-scattered diffraction (EBSD) technique. 展开更多
关键词 Ti-22Al-25Nb hot deformation constitutive relation EBSD technique
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Toll样受体4(TLR4)基因剔除小鼠构建及初步表型分析 被引量:5
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作者 郭洋 万颖寒 +7 位作者 王珏 龚慧 周宇 慈磊 万志鹏 孙瑞林 费俭 沈如凌 《中国生物工程杂志》 CAS CSCD 北大核心 2020年第6期1-9,共9页
目的:利用CRISPR/Cas9技术构建Toll样受体4(TLR4)基因敲除小鼠模型,并观察突变小鼠对革兰氏阴性细菌脂多糖(LPS)刺激响应的变化。方法:针对TLR4基因外显子2设计并合成1对sgRNA片段,与编码Cas9的mRNA混合后通过受精卵显微注射方法,建立T... 目的:利用CRISPR/Cas9技术构建Toll样受体4(TLR4)基因敲除小鼠模型,并观察突变小鼠对革兰氏阴性细菌脂多糖(LPS)刺激响应的变化。方法:针对TLR4基因外显子2设计并合成1对sgRNA片段,与编码Cas9的mRNA混合后通过受精卵显微注射方法,建立TLR4基因敲除小鼠,通过繁育获得基因敲除纯合子小鼠(TLR4-/-小鼠);通过LPS刺激,分析TLR4-/-小鼠对炎症应激的反应情况,并在分子和病理水平上和野生型对照(WT)进行比较。结果:PCR及测序检测表明TLR4基因外显子2在小鼠基因中被成功敲除;给予LPS刺激后,IL1β、IL6、My D88、i NOS及TNFa等炎症因子的表达在野生型小鼠的心、肝和肺组织中显著上调,而在TLR4-/-小鼠中则几乎没有变化;血生化指标显示LPS刺激后WT小鼠血清中的尿素(Urea)和肌酐(Cre)水平显著升高,而TLR4-/-小鼠刺激前后无显著变化,病理分析同样发现TLR4-/-小鼠能够抵抗LPS对肾组织的损伤。结论:利用CRISPR/Cas9技术成功构建了TLR4基因剔除小鼠模型,TLR4的缺失能够降低IL1β、IL6、My D88、i NOS及TNFa炎症因子对LPS刺激的响应,抑制LPS引起的炎症反应及对组织的损伤。 展开更多
关键词 Toll样受体4(TLR4) CRISPR/Cas9系统 基因敲除 LPS
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