Dear Editor,I am Dr Xing-Chao Shentu,from the Eye Center of the Second Affiliated Hospital,Medical College of Zhejiang University,Hangzhou,China.I write to present case series of differences in intraocular lens (IOL)p...Dear Editor,I am Dr Xing-Chao Shentu,from the Eye Center of the Second Affiliated Hospital,Medical College of Zhejiang University,Hangzhou,China.I write to present case series of differences in intraocular lens (IOL)power calculation by partial coherence interferometry (PCI)and ultrasound A-scan biometry with sub-foveal choroidal neovascularization (CNV).展开更多
Aim The piezoelectric properties and cytotoxicity of a porous lead-free piezoelectric ceramic for use as a direct bone substitute were investigated. Methodology Cold isostatic pressing （CIP） was applied to fabricate...Aim The piezoelectric properties and cytotoxicity of a porous lead-free piezoelectric ceramic for use as a direct bone substitute were investigated. Methodology Cold isostatic pressing （CIP） was applied to fabricate porous lithium sodium potassium niobate （Li0.06Na0.5K0.44） NbO3 specimens using a pore-forming method. The morphologies of the CIP-processed specimens were characterized and compared to those of specimens made by from conventional pressing procedures. The effects of the ceramic on the attachment and proliferation of osteoblasts isolated from the cranium of 1-day-old Sprague- Dawley rats were examined by a scanning electron microscopy （SEM） and metbylthiazol tetrazolium （MTT） assay. Results The results showed that CIP enhanced piezoelectricity and biological performance of the niobate specimen, and also promoted an extracellular matrix-like topography of it. In vitro studies showed that the CIP-enhanced material had positive effects on the attachment and proliferation of osteoblasts. Conclusion Niobate ceramic generated by CIP shows a promise for being a piezoelectric composite bone substitute.展开更多
BACKGROUND Coronary microembolization(CME)is a complicated problem that commonly arises in the context of coronary angioplasty.MicroRNAs play crucial roles in cardiovascular diseases.However,the role and mechanism of ...BACKGROUND Coronary microembolization(CME)is a complicated problem that commonly arises in the context of coronary angioplasty.MicroRNAs play crucial roles in cardiovascular diseases.However,the role and mechanism of miR-181a-5p in CME-induced myocardial injury remains unclear.METHODS We established CME rat models.Cardiac function was detected by echocardiography.Haematoxylin-basic fuchsin-picric acid staining was used to measure micro-infarction size.Serum samples and cell culture supernatants were evaluated via enzyme-linked immunosorbent assay.Cellular reactive oxygen species were determined by dichloro-dihydro-fluorescein diacetate assay,and the other oxidative stress related parameters were assayed by spectrophotometry.The dual-luciferase reporter(DLR)assay and RNA pulldown were conducted to validate the association between miR-181a-5p and X-linked inhibitor of apoptosis protein(XIAP).The expression of miR-181a-5p and XIAP mRNA were determined by quantitative reverse transcription polymerase chain reaction.Proteins were evaluated via immunoblotting.The viability of the cell was evaluated via cell counting kit-8 assay.RESULTS The miR-181a-5p level was significantly increased in CME myocardial tissues.Downregulation of miR-181a-5p improved CME-induced cardiac dysfunction and alleviated myocardial oxidative stress and inflammatory injury,whereas miR-181a-5p exhibited the opposite effects.Then,the DLR assay and RNA pulldown results revealed that miR-181a-5p directly targeting on XIAP.The XIAP level was found to be remarkably decreased after CME.XIAP overexpression attenuated CME-induced myocardial oxidative stress and inflammatory injury.Finally,in vitro rescue experiments revealed that knockdown of XIAP could abolish the protective effects of miR-181a-5p knockdown on hypoxia-induced cardiomyocyte oxidative stress and inflammatory injury.CONCLUSIONS Downregulation of miR-181a-5p alleviates CME-induced myocardial damage by suppressing myocardial oxidative stress and inflammation through directly targeting XIAP.展开更多
Dear sir,I am Dr Zhi-Qing Chen, from the Department of Ophthalmology of the Second Affiliated Hospital ofZhejiang University in Hangzhou, Zhejiang Province, China. I write to present two cases of neovascular age-relat...Dear sir,I am Dr Zhi-Qing Chen, from the Department of Ophthalmology of the Second Affiliated Hospital ofZhejiang University in Hangzhou, Zhejiang Province, China. I write to present two cases of neovascular age-related macular degeneration （AMD） occurred retinal pigment epithelium （RPE） tears after different treatment.展开更多
Choroidal neovascularization(CNV)is an uncommon complication associated with a macular hole.In this case report of a rare condition,we present a pathologic myopia patient with a co-existent macular hole and choroidal ...Choroidal neovascularization(CNV)is an uncommon complication associated with a macular hole.In this case report of a rare condition,we present a pathologic myopia patient with a co-existent macular hole and choroidal neovascular membrane.The patient was treated with photodynamic therapy for CNV,and then vitreous surgery for the retinal detachment and macular hole.At the end of 4 years follow-up,her visual acuity was improved to 0.1 while the macular hole remained open.Optical coherence tomography is a useful inspection method of the diagnosis of CNV and macular hole.展开更多
Dear Editor,I am Dr.Zhi-Tao Su,from the Eye Center,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou,China.I write to present a case of spontaneous resolution of a traumatic cataract resulted ...Dear Editor,I am Dr.Zhi-Tao Su,from the Eye Center,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou,China.I write to present a case of spontaneous resolution of a traumatic cataract resulted in good visual rehabilitation after removal of a ferrous intravitreal foreign body(IVFB).展开更多
基金the foundation from the National Key Research and Development Program of China (No. 2020YFB1505901)the support by Xiao-qin ZENG Expert Workstation in Yunnan Province, China (No. 202005AF150059)。
基金Supported by the National Natural Science Foundation of China (No.81500766 No.81371000 No.81670834)+1 种基金Natural Science Foundation of Zhejiang Province (No.LY16H120002)the Foundation from Health and Family Planning Commission of Zhejiang Province (No.201347434)
文摘Dear Editor,I am Dr Xing-Chao Shentu,from the Eye Center of the Second Affiliated Hospital,Medical College of Zhejiang University,Hangzhou,China.I write to present case series of differences in intraocular lens (IOL)power calculation by partial coherence interferometry (PCI)and ultrasound A-scan biometry with sub-foveal choroidal neovascularization (CNV).
基金supported by the National Natural Science Foundation of China (59972020)
文摘Aim The piezoelectric properties and cytotoxicity of a porous lead-free piezoelectric ceramic for use as a direct bone substitute were investigated. Methodology Cold isostatic pressing （CIP） was applied to fabricate porous lithium sodium potassium niobate （Li0.06Na0.5K0.44） NbO3 specimens using a pore-forming method. The morphologies of the CIP-processed specimens were characterized and compared to those of specimens made by from conventional pressing procedures. The effects of the ceramic on the attachment and proliferation of osteoblasts isolated from the cranium of 1-day-old Sprague- Dawley rats were examined by a scanning electron microscopy （SEM） and metbylthiazol tetrazolium （MTT） assay. Results The results showed that CIP enhanced piezoelectricity and biological performance of the niobate specimen, and also promoted an extracellular matrix-like topography of it. In vitro studies showed that the CIP-enhanced material had positive effects on the attachment and proliferation of osteoblasts. Conclusion Niobate ceramic generated by CIP shows a promise for being a piezoelectric composite bone substitute.
基金This study was supported by the National Natural Science Foundation of China(No.81770346)the Project for Innovative Research Team in Guangxi Natural Science Foundation(2018GXNSFGA281006).
文摘BACKGROUND Coronary microembolization(CME)is a complicated problem that commonly arises in the context of coronary angioplasty.MicroRNAs play crucial roles in cardiovascular diseases.However,the role and mechanism of miR-181a-5p in CME-induced myocardial injury remains unclear.METHODS We established CME rat models.Cardiac function was detected by echocardiography.Haematoxylin-basic fuchsin-picric acid staining was used to measure micro-infarction size.Serum samples and cell culture supernatants were evaluated via enzyme-linked immunosorbent assay.Cellular reactive oxygen species were determined by dichloro-dihydro-fluorescein diacetate assay,and the other oxidative stress related parameters were assayed by spectrophotometry.The dual-luciferase reporter(DLR)assay and RNA pulldown were conducted to validate the association between miR-181a-5p and X-linked inhibitor of apoptosis protein(XIAP).The expression of miR-181a-5p and XIAP mRNA were determined by quantitative reverse transcription polymerase chain reaction.Proteins were evaluated via immunoblotting.The viability of the cell was evaluated via cell counting kit-8 assay.RESULTS The miR-181a-5p level was significantly increased in CME myocardial tissues.Downregulation of miR-181a-5p improved CME-induced cardiac dysfunction and alleviated myocardial oxidative stress and inflammatory injury,whereas miR-181a-5p exhibited the opposite effects.Then,the DLR assay and RNA pulldown results revealed that miR-181a-5p directly targeting on XIAP.The XIAP level was found to be remarkably decreased after CME.XIAP overexpression attenuated CME-induced myocardial oxidative stress and inflammatory injury.Finally,in vitro rescue experiments revealed that knockdown of XIAP could abolish the protective effects of miR-181a-5p knockdown on hypoxia-induced cardiomyocyte oxidative stress and inflammatory injury.CONCLUSIONS Downregulation of miR-181a-5p alleviates CME-induced myocardial damage by suppressing myocardial oxidative stress and inflammation through directly targeting XIAP.
基金Supported by the Natural Science Foundation of Zhejiang Province(No.LY 16H 120002)Zhejiang Province Medical Platform Program Grant(No.2013RCB008)
文摘Dear sir,I am Dr Zhi-Qing Chen, from the Department of Ophthalmology of the Second Affiliated Hospital ofZhejiang University in Hangzhou, Zhejiang Province, China. I write to present two cases of neovascular age-related macular degeneration （AMD） occurred retinal pigment epithelium （RPE） tears after different treatment.
基金Public Welfare Technology Project of Science Technology Department of Zhejiang Province,China(No.2012C23068)
文摘Choroidal neovascularization(CNV)is an uncommon complication associated with a macular hole.In this case report of a rare condition,we present a pathologic myopia patient with a co-existent macular hole and choroidal neovascular membrane.The patient was treated with photodynamic therapy for CNV,and then vitreous surgery for the retinal detachment and macular hole.At the end of 4 years follow-up,her visual acuity was improved to 0.1 while the macular hole remained open.Optical coherence tomography is a useful inspection method of the diagnosis of CNV and macular hole.
基金Supported by the National Natural Science Foundation of China(No.81800877)the Natural Science Foundation of Zhejiang Province(No.LY15H120001No.LQ17H120008)。
文摘Dear Editor,I am Dr.Zhi-Tao Su,from the Eye Center,Second Affiliated Hospital,School of Medicine,Zhejiang University,Hangzhou,China.I write to present a case of spontaneous resolution of a traumatic cataract resulted in good visual rehabilitation after removal of a ferrous intravitreal foreign body(IVFB).
