Plastid phylogenomics provides new insights into the systematics,diversification,and biogeography of Cymbidium(Orchidaceae)

Cymbidium(Orchidaceae:Epidendroideae),with around 60 species,is widely-distributed across Southeast Asia,providing a nice system for studying the processes that underlie patterns of biodiversity in the region.However,phylogenetic relationships of Cymbidium have not been well resolved,hampering investigations of species diversification and the biogeographical history of this genus.In this study,we construct a plastome phylogeny of 56 Cymbidium species,with four well-resolved major clades,which provides a framework for biogeographical and diversification rate analyses.Molecular dating and biogeographical analyses show that Cymbidium likely originated in the region spanning northern IndoBurma to the eastern Himalayas during the early Miocene(~21.10 Ma).It then rapidly diversified into four major clades in East Asia within approximately a million years during the middle Miocene.Cymbidium spp.migration to the adjacent regions(Borneo,Philippines,and Sulawesi)primarily occurred during the Pliocene-Pleistocene period.Our analyses indicate that the net diversification rate of Cymbidium has decreased since its origin,and is positively associated with changes in temperature and monsoon intensity.Favorable hydrothermal conditions brought by monsoon intensification in the early Miocene possibly contributed to the initial rapid diversification,after which the net diversification rate was reduced with the cooling climate after the middle Miocene.The transition from epiphytic to terrestrial habits may have enabled adaptation to cooler environments and colonization of northern niches,yet without a significant effect on diversification rates.This study provides new insights into how monsoon activity and temperature changes affected the diversification dynamics of plants in Southeast Asia.

Recurrent multisystem Langerhans cell histiocytosis involving the female genitalia: A case report

BACKGROUND Langerhans cell histiocytosis(LCH)is a histiocytic proliferative disease caused by clonal proliferation of Langerhans cells,which is currently defined as an inflam-matory myeloid tumor.It is rare in adults,with an incidence of 1–2 per million,and is highly heterogeneous in clinical presentation,with unpredictable disease progression and outcome.CASE SUMMARY A 52-year-old postmenopausal female patient presented to the gynecology department in July 2023 with bilateral vulvar masses.She was diagnosed with recurrent multisystem LCH.The patient had previously been diagnosed with a single-system and single-focal LCH in October 2021 due to a right maxillofacial mass,which resolved after surgical treatment.A chemotherapy regimen was developed after multidisciplinary consultation.Six cycles of chemotherapy resulted in partial remission,and maintenance chemotherapy is currently being administered.CONCLUSION Recurrent LCH involving the bilateral vulva has been poorly reported.Compre-hensive imaging and pathological evaluation is important for diagnosis.The model of joint multidisciplinary specialist diagnosis and treatment is worthy of clinical application.

Hemophagocytic lymphohistiocytosis after autologous stem cell transplantation in angioimmunoblastic T-cell lymphoma:A case report

BACKGROUND Angioimmunoblastic T-cell lymphoma(AITL), a unique subtype of peripheral Tcell lymphoma, has relatively poor outcomes. High-dose chemotherapy with autologous stem cell transplantation(ASCT) can achieve complete remission and improve outcomes. Unfortunately, subsequent T-cell lymphoma-triggered hemophagocytic lymphohistiocytosis(HLH) has a worse prognosis than B-cell lymphoma-triggered HLH.CASE SUMMARY We here report a 50-year-old woman with AITL who achieved a favorable outcome after developing HLH 2 mo after receiving high-dose chemotherapy/ASCT. The patient was initially admitted to our hospital because of multiple enlarged lymph nodes. The final pathologic diagnosis, made on biopsy of a left axillary lymph node was AITL(Stage Ⅳ, Group A). Four cycles of the following chemotherapy regimen were administered: Cyclophosphamide 1.3 g, doxorubicin 86 mg, and vincristine 2 mg on day 1;prednisone 100 mg on days 1-5;and lenalidomide 25 mg on days 1-14. The interval between each cycle was 21 d. The patient received a conditioning regimen(busulfan, cyclophosphamide, and etoposide) followed by peripheral blood stem cell infusion. Unfortunately, she developed sustained fever and a low platelet count 17 d after ACST, leading to a diagnosis of HLH after ASCT. During treatment, she experienced thrombocytopenia and Pneumocystis carinii pneumonia. The patient was successfully treated with etoposide and glucocorticoids.CONCLUSION It is possible that development of HLH is related to immune reconstitution after ASCT.

Four new species and a new record of Orchidinae(Orchidaceae:Orchideae)from China

Four new species of Orchidaceae from China,Heminium lijiangense,Peristylus fasciculatus,Platanthera milinensis,and Ponerorchis gongshanensis,together with a new country record,Peristylus tenuicallus,are described and illustrated based on morphological and/or phylogenetic analyses.Heminium lijiangense is closely related to H.elisabethae but differs from it by having the dorsal sepal ovate-orbicular and lip midlobe distinctly shorter than lateral lobes.P.fasciculatus is close to Peristylus tradescantifolius but is distinguished from it by having several fascicled and straight,root-like tubers(vs.one or two oblongoid tubers),old stems usually persistent,middle lobe of lip narrowly ligulate-lanceolate and half as long as the lateral lobes(vs.middle lobe deltoid,about a third as long as the lateral lobes or less),a raised callus at the base of each lateral lobe(vs.callus absent),spur gradually attenuate toward the apex(vs.spur clavate).Platanthera milinensis is similar to P.stenochila by sharing small green flowers and lip without a spur,but differs in having a creeping rhizome,a corymbose inflorescence,and a broadly ovate and slightly 3-lobed lip.Ponerochis gongshanensis is similar to P.faberi in its small flowers,but differs in having a linear leaf c.3 mm wide(vs.leaf 5-13 mm wide),in the lip having collar-like raised margins on the sides of the spur entrance,and a mid-lobe which is notched at the apex but not divided into two divergent lobules that are nearly as large as the lateral lobes,as in P.faberi.All the proposed species obtained high support in phylogenetic analysis as new species.The recently described genus Apetalanthe is reduced to synonymy of Ponerorchis and a new combination is made.

Three new species of Liparis s.l.(Orchidaceae:Malaxideae)from Southwest China based on morphological characters and phylogenetic evidence

Liparis aureolabella and L mengziensis,two new species from the karst region of southwestern China,and L bingzhongluoensis,a new species from montane region in Yunnan,are described and illustrated.L aureolabella is easily distinguished from its relatives by having abaxially purple leave with purple reticulate veins prominent adaxially,a lip auriculate at base,and falcate-lanceolate pollinia.Liparis mengziensis is closely related to L.petiolata and L.auriculata,but differs from them by having an ovate to broadly ovate leaf,purple lip and apex connate along the margins.Liparis bingzhongluoensis is similar to Liparis nanlingensis,but the new species is characterized by having a lip with two transparent ridges on its disc,longitudinally concave basal callus and triangular column wings.Phylogenetic analyses based on nuclear ribosomal ITS and plastid matK sequences showed that L aureolabella and L mengziensis are nested with L petiolata or L auriculata in a monophyletic clade.L bingzhongluoensis is sister to a clade formed by L.nanlingensis,L.tsii,L sasakii and L.krameri.Moreover,morphological comparisons strongly support that the three species as separated species newly to science.

Simultaneous determination of human plasma protein binding of bioactive favonoids in Polygonum orientale by equilibrium dialysis combined with UPLC–MS/MS

A simple and selective ultra performance liquid chromatography--electrospray ionization tandem mass spectrometry （UPLC-ESI-MS/MS） assay was developed for the determination of the human plasma protein binding of four bioactive ftavonoids （such as orientin and vitexin） in Polygonum orientale. Protein precipitation was used for sample preparation. Equilibrium dialysis technique was applied to determine the plasma protein binding under physiological conditions. The separation was achieved through a Waters C i s column with a mobile phase composed of 0.1% formic acid in acetonitrile and 0.1% aqueous formic acid using step gradient elution at a flow rate of 0.35 mL/min. A Waters ACQUITYTM TQD system was operated under the multiple reaction monitoring （MRM） mode of positive electrospray ionization. All of the recovery, precision, accuracy and stability of the method met the requirements. Good correlations （r 〉 0.99） of the four compounds were found, which suggested that these compounds can be simultaneously determined with acceptable accuracy. Results showed that the plasma protein bindings of the four bioactive flavonoids were in the range of 74-89% over the six concentrations studied. The binding parameters containing protein binding affinity, protein binding dissociation constant, and protein binding site were studied. The maximum ability to bind with protein was also determined in the assay in order to understand the drug-protein binding of each compound better.

Two genetically distinct units of Sinomanglietia glauca (Magnoliaceae) detected by chloroplast PCR-SSCP

Sinomanglietia glauca is a critically endangered species described from Jiangxi Province in the 1990s. Recently two populations were discovered from Yongshun County of west Hunan Province, about 450 km away from those in Jiangxi. Because of the new findings and the poor reproducibility inherent to RAPD and ISSR markers of previous studies, the population structure of this rare species was reanalyzed with chloroplast PCR-SSCP （single-stranded conformation polymorphism）, including all of four recorded populations. The results showed that two distinct haplotypes characterized Jiangxi and Hunan populations separately, with no genetic variation occurring within regions. We postulated that this surprising pattern might result from habitat fragmenta- tion and demographic bottlenecks during and/or after the Quaternary glaciation. On the basis of the pronounced genetic structure, two evolutionarily significant units （ESUs） were recommended for effective conservation of S. glauca.

Extracorporeal membrane oxygenation treatment for high-risk pulmonary embolism with cardiac arrest in a young adult male

Dear editor,A 21-year-old male student was admitted to the emergency department of our hospital due to chest distress,dyspnea for 1.5 hours, and loss of consciousness for one minute. Before admission, the patient had been advised rest for two months because of left ankle sprain, leading to less activity. At admission, the patient was unconscious,with facial cyanosis, and his limbs were cold.

Performance analysis of GaN-based high-electron-mobility transistors with postpassivation plasma treatment

AlGaN/GaN high-electron-mobility transistors(HEMTs)with postpassivation plasma treatment are demonstrated and investigated for the first time.The results show that postpassivation plasma treatment can reduce the gate leakage and enhance the drain current.Comparing with the conventional devices,the gate leakage of Al Ga N/Ga N HEMTs with postpassivation plasma decreases greatly while the drain current increases.Capacitance-voltage measurement and frequencydependent conductance method are used to study the surface and interface traps.The mechanism analysis indicates that the surface traps in the access region can be reduced by postpassivation plasma treatment and thus suppress the effect of virtual gate,which can explain the improvement of DC characteristics of devices.Moreover,the density and time constant of interface traps under the gate are extracted and analyzed.

Molecular Dynamics Simulation of MgO Melting at High Pressure

Molecular dynamics simulation was used to study the melting of MgO at high pressures. The melting temperature of MgO was accurately obtained at elevated temperature and high pressure after corrections based on the modern theory of melting. The calculated melting curve was compared with the available experimental data and other theoretical results at the pressure range of 0-135 GPa. The corrected melting temperature of MgO is in good agreement with the results from Lindemann melting equation and the two- phase simulated results below 15 GPa.

Traumatic tension pneumocephalus:A case report

Dear editor,Traumatic pneumocephalus describes the presence of gas in the brain cavity,with an incidence of 0.5%–9.7%.^([1])It was first reported by Luckett.^([2])It is primarily self-limiting.However,traumatic tension pneumocephalus(TTP)due to increased intracranial gas is extremely rare.It is a neurological emergency manifested by headaches,seizures,loss of consciousness,and even death resulting from high intracranial pressure.

Nuclear phylogenomics of Asteraceae with increased sampling provides new insights into convergent morphological and molecular evolution

Convergent morphological evolution is widespread in flowering plants,and understanding this phenomenon relies on well-resolved phylogenies.Nuclear phylogenetic reconstruction using transcriptome datasets has been successful in various angiosperm groups,but it is limited to taxa with available fresh materials.Asteraceae,which are one of the two largest angiosperm families and are important for both ecosystems and human livelihood,show multiple examples of convergent evolution.Nuclear Asteraceae phylogenies have resolved relationships among most subfamilies and many tribes,but many phylogenetic and evolutionary questions regarding subtribes and genera remain,owing to limited sampling.Here,we increased the sampling for Asteraceae phylogenetic reconstruction using transcriptomes and genome-skimming datasets and produced nuclear phylogenetic trees with 706 species representing two-thirds of recognized subtribes.Ancestral character reconstruction supports multiple convergent evolutionary events in Asteraceae,with gains and losses of bilateral floral symmetry correlated with diversification of some subfamilies and smaller groups,respectively.Presence of the calyx-related pappus may have been especially important for the success of some subtribes and genera.Molecular evolutionary analyses support the likely contribution of duplications of MADS-box and TCP floral regulatory genes to innovations in floral morphology,including capitulum inflorescences and bilaterally symmetric flowers,potentially promoting the diversification of Asteraceae.Subsequent divergences and reductions in CYC2 gene expression are related to the gain and loss of zygomorphic flowers.This phylogenomic work with greater taxon sampling through inclusion of genome-skimming datasets reveals the feasibility of expanded evolutionary analyses using DNA samples for understanding convergent evolution.

An injectable micelle-hydrogel hybrid for localized and prolonged drug delivery in the management of renal fibrosis

Localized delivery,comparing to systemic drug administration,offers a unique alternative to enhance efficacy,lower dosage,and minimize systemic tissue toxicity by releasing therapeutics locally and specifically to the site of interests.Herein,a localized drug delivery platform ("plum?pudding"structure) with controlled release and long-acting features is developed through an injectable hydrogel ("pudding") crosslinked via self-assembled triblock polymeric micelles ("plum") to help reduce renal interstitial fibrosis.This strategy achieves controlled and prolonged release of model therapeutics in the kidney for up to three weeks in mice.Following a single injection,local treatments containing either anti-inflammatory small molecule celastrol or anti-TGFb antibody effectively minimize inflammation while alleviating fibrosis via inhibiting NF-k B signaling pathway or neutralizing TGF-b1 locally.Importantly,the micelle-hydrogel hybrid based localized therapy shows enhanced efficacy without local or systemic toxicity,which may represent a clinically relevant delivery platform in the management of renal interstitial fibrosis.

An integral calculation approach for numerical simulation of cavitating flow around a marine propeller behind the ship hull

In this paper, the unsteady cavitating turbulent flow around a marine propeller is simulated based on the unsteady Reynolds averaged Navier-Stokes(URANS) with emphasis on the hull-propeller interaction by an integral calculation approach, which means the propeller and hull are treated as a whole when the cavitating flow is calculated. The whole calculational domain is split to an inner rotating domain containing a propeller and an outer domain containing a hull. And the two split sections are connected together in ANSYS CFX by using the GGI interfaces and the transient rotor stator frame change/mixing model. The alternate rotation model is employed for the advection term in the momentum equations in order to reduce the numerical error. Comparison of predictions with measurements shows that the propeller thrust coefficient can be predicted satisfactorily. The unsteady cavitating flow around the propeller behind the ship hull wake shows quasi-periodic features including cavity inception, growth and shrinking. These features are effectively reproduced in the simulations which compare well to available experimental data. In addition, significant pressure fluctuations on the ship hull surface induced by the unsteady propeller cavitation are compared with experimental data at monitoring points on the hull surface. The predicted amplitudes of the first components corresponding to the first blade passing frequencies match well with the experimental data. The maximum error between the predictions and the experimental data for the pressure pulsations is around 8%, which is acceptable in most engineering applications.