The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V ...The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V CRISPR-Cpfl （also known as Cas12a） system is a two-component transcriptional unit （TCTU） in which the Cas9 or Cpf1 protein is expressed from an RNA polymerase （pol） II promoter, whereas the single guide RNA （sgRNA） is typically expressed from a Pol III promoter, such as U6 or U3 promoter.展开更多
Let I_(α,→b)be the multilinear commutators of the fractional integrals Iαwith the symbol→b=(b1,……,bk)We show that the constant of borderline weighted estimates for Iαis I/ξ,and for I_(α,→b)is with each b_(i)...Let I_(α,→b)be the multilinear commutators of the fractional integrals Iαwith the symbol→b=(b1,……,bk)We show that the constant of borderline weighted estimates for Iαis I/ξ,and for I_(α,→b)is with each b_(i)belongs to the Orlicz space Osc_(exp L^(si)).展开更多
Maintenance of cell wall integrity is of great importance not only for plant growth and development,but also for the adaptation of plants to adverse environments.However,how the cell wall integrity is modulated under ...Maintenance of cell wall integrity is of great importance not only for plant growth and development,but also for the adaptation of plants to adverse environments.However,how the cell wall integrity is modulated under salt stress is still poorly understood.Here,we report that a nuclear-localized Agenet domain-containing protein SWO1(SWOLLEN 1)is required for the maintenance of cell wall integrity in Arabidopsis under salt stress.Mutation in SWO1 gene results in swollen root tips,disordered root cell morphology,and root elongation inhibition under salt stress.The swo1 mutant accumulates less cellulose and pectin but more lignin under high salinity.RNA-seq and ChIP-seq assays reveal that SWO1 binds to the promoter of several cell wall-related genes and regulates their expression under saline conditions.Further study indicates that SWO1 interacts with importinɑIMPA1 and IMPA2,which are required for the import of nuclear-localized proteins.The impa1 impa2 double mutant also exhibits root growth inhibition under salt stress and mutations of these two genes aggravate the salt-hypersensitive phenotype of the swo1 mutant.Taken together,our data suggest that SWO1 functions together with importinɑto regulate the expression of cell wall-related genes,which enables plants to maintain cell wall integrity under high salinity.展开更多
文摘The class 2 clustered regularly interspaced short palindromic repeat （CRISPR） systems have been widely used for simultaneous modification of multiple loci in plants. Traditionally, the type II CRISPR-Cas9 or type V CRISPR-Cpfl （also known as Cas12a） system is a two-component transcriptional unit （TCTU） in which the Cas9 or Cpf1 protein is expressed from an RNA polymerase （pol） II promoter, whereas the single guide RNA （sgRNA） is typically expressed from a Pol III promoter, such as U6 or U3 promoter.
文摘Let I_(α,→b)be the multilinear commutators of the fractional integrals Iαwith the symbol→b=(b1,……,bk)We show that the constant of borderline weighted estimates for Iαis I/ξ,and for I_(α,→b)is with each b_(i)belongs to the Orlicz space Osc_(exp L^(si)).
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences,Grant XDB27040101(to J-K.Z.)Shanghai Pujiang Program,Grant 20PJ1414800(to C.Z.),and National Natural Science Foundation of China,Grant No.32070295(to C.Z.).
文摘Maintenance of cell wall integrity is of great importance not only for plant growth and development,but also for the adaptation of plants to adverse environments.However,how the cell wall integrity is modulated under salt stress is still poorly understood.Here,we report that a nuclear-localized Agenet domain-containing protein SWO1(SWOLLEN 1)is required for the maintenance of cell wall integrity in Arabidopsis under salt stress.Mutation in SWO1 gene results in swollen root tips,disordered root cell morphology,and root elongation inhibition under salt stress.The swo1 mutant accumulates less cellulose and pectin but more lignin under high salinity.RNA-seq and ChIP-seq assays reveal that SWO1 binds to the promoter of several cell wall-related genes and regulates their expression under saline conditions.Further study indicates that SWO1 interacts with importinɑIMPA1 and IMPA2,which are required for the import of nuclear-localized proteins.The impa1 impa2 double mutant also exhibits root growth inhibition under salt stress and mutations of these two genes aggravate the salt-hypersensitive phenotype of the swo1 mutant.Taken together,our data suggest that SWO1 functions together with importinɑto regulate the expression of cell wall-related genes,which enables plants to maintain cell wall integrity under high salinity.
