Recent studies have identified olfactory ecto-mesenchymal stem cells （OE-MSCs） as a new type of resident stem cell in the olfactory lamina propria. However, it remains unclear whether OE-MSCs possess any immunoregul...Recent studies have identified olfactory ecto-mesenchymal stem cells （OE-MSCs） as a new type of resident stem cell in the olfactory lamina propria. However, it remains unclear whether OE-MSCs possess any immunoregulatory functions. In this study, we found that mouse OE-MSCs expressed higher transforming growth factor-beta and interleukin-10 levels than bone marrow-derived MSCs. In culture, OE-MSCs exerted their immunosuppressive capacity via directly suppressing effector T-cell proliferation and increasing regulatory T （Treg） cell expansion. In mice with collagen-induced arthritis, adoptive transfer of OE-MSCs markedly suppressed arthritis onset and disease severity, which was accompanied by increased Treg cells and reduced Th1/Th17 cell responses in vivo. Taken together, our findings identified a novel function of OE-MSCs in regulating T-cell responses, indicating that OE-MSCs may represent a new cell therapy for the treatment of rheumatoid arthritis and other autoimmune diseases.展开更多
Hepatitis E virus(HEV)infection is a common public health problem in developing countries.However,the current prevalence of HEV and the relationship of HEV genotype between swine and human within high-density pig-farm...Hepatitis E virus(HEV)infection is a common public health problem in developing countries.However,the current prevalence of HEV and the relationship of HEV genotype between swine and human within high-density pig-farming areas in central China are still inadequately understood.Here,cross-sectional serological and genotypic surveys of HEV among the 1232 general population,273 workers occupationally exposed to swine,and 276 pigs in a high-density pig-breeding area,were undertaken by ELISA and nested RT-PCR methods.Anti-HEV IgG was detected in 26.22%of general population and 48.35%of occupational workers.The prevalence of swine serum HEV-Ag was 6.52%.The prevalence of anti-HEV IgG was significantly higher among the workers occupationally exposed to swine than among the general population.An increased HEV seropositivity risk among the general population was associated with either being a peasant or male and was very strongly associated with the increase of age.Among the occupationally exposed group,the prevalence of anti-HEV IgG antibodies increased with age and working years.Among the 30 HEV-IgM-positive people,the infection rates of clerks in the public,peasants,pork retailers,and pig farmers were higher than those of others.A phylogenetic analysis revealed that all the isolates belonged to subgenotype 4d,and four people and four pigs shared 97.04%-100%sequence homology.This study revealed a high HEV seroprevalence among the general population and workers occupationally exposed to swine in the Anlu City,and supports the notion that swine are a source of human HEV infection.展开更多
On the basis of the proposed structures of jiangrines C and D, a synthetic strategy was initiated from D-glyceraldehyde acetonide,a readily available chiral material. Through a linear seven-step synthesis, the target ...On the basis of the proposed structures of jiangrines C and D, a synthetic strategy was initiated from D-glyceraldehyde acetonide,a readily available chiral material. Through a linear seven-step synthesis, the target molecules were accomplished. However, all characteristic data of the synthetic 3 and 4 were found to be different from those of natural jiangrines C and D. Accordingly, the molecular structures of jiangrines should be revised and a possible molecular skeleton for them was proposed.展开更多
基金This work was supported by grants from the National Natural Science Foundation of China （Grant Nos. 31470881, 81072453）, the Specialized Research Fund for the Doctoral Program of Higher Education （Grant No. 20133227110008）, the Health Department Foundation of Jiangsu Province （Grant No. Z201312）, the Graduate Student Research and the Innovation Program of Jiangsu Province （Grant No. KYLX_1074）, the Jiangsu Province ＇333＇ Project （Grant No. BRA2015197 ）, the Priority Academic Program Development of Jiangsu Higher Education Institutions, the National Basic Research Program of China （2014CB541904）, and the Hong Kong Croucher Foundation.
文摘Recent studies have identified olfactory ecto-mesenchymal stem cells （OE-MSCs） as a new type of resident stem cell in the olfactory lamina propria. However, it remains unclear whether OE-MSCs possess any immunoregulatory functions. In this study, we found that mouse OE-MSCs expressed higher transforming growth factor-beta and interleukin-10 levels than bone marrow-derived MSCs. In culture, OE-MSCs exerted their immunosuppressive capacity via directly suppressing effector T-cell proliferation and increasing regulatory T （Treg） cell expansion. In mice with collagen-induced arthritis, adoptive transfer of OE-MSCs markedly suppressed arthritis onset and disease severity, which was accompanied by increased Treg cells and reduced Th1/Th17 cell responses in vivo. Taken together, our findings identified a novel function of OE-MSCs in regulating T-cell responses, indicating that OE-MSCs may represent a new cell therapy for the treatment of rheumatoid arthritis and other autoimmune diseases.
基金partly supported by General Projects of Health and Family Planning Commission of Hubei Province of China no. WJ2017M174, and WJ2017M240Occupational Hazard and Identification Control of Hubei Provincial Key Laboratory Open Fund, no. OCHI2017G02
文摘Hepatitis E virus(HEV)infection is a common public health problem in developing countries.However,the current prevalence of HEV and the relationship of HEV genotype between swine and human within high-density pig-farming areas in central China are still inadequately understood.Here,cross-sectional serological and genotypic surveys of HEV among the 1232 general population,273 workers occupationally exposed to swine,and 276 pigs in a high-density pig-breeding area,were undertaken by ELISA and nested RT-PCR methods.Anti-HEV IgG was detected in 26.22%of general population and 48.35%of occupational workers.The prevalence of swine serum HEV-Ag was 6.52%.The prevalence of anti-HEV IgG was significantly higher among the workers occupationally exposed to swine than among the general population.An increased HEV seropositivity risk among the general population was associated with either being a peasant or male and was very strongly associated with the increase of age.Among the occupationally exposed group,the prevalence of anti-HEV IgG antibodies increased with age and working years.Among the 30 HEV-IgM-positive people,the infection rates of clerks in the public,peasants,pork retailers,and pig farmers were higher than those of others.A phylogenetic analysis revealed that all the isolates belonged to subgenotype 4d,and four people and four pigs shared 97.04%-100%sequence homology.This study revealed a high HEV seroprevalence among the general population and workers occupationally exposed to swine in the Anlu City,and supports the notion that swine are a source of human HEV infection.
基金supported by the National Natural Science Foundation of China (21290180, 21322205, 21321061)the Open Fund of State Key Laboratory of Natural Medicines (SKLNMKF201601)
文摘On the basis of the proposed structures of jiangrines C and D, a synthetic strategy was initiated from D-glyceraldehyde acetonide,a readily available chiral material. Through a linear seven-step synthesis, the target molecules were accomplished. However, all characteristic data of the synthetic 3 and 4 were found to be different from those of natural jiangrines C and D. Accordingly, the molecular structures of jiangrines should be revised and a possible molecular skeleton for them was proposed.
