DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years.In this study,we developed a single-molecule desktop sequencer,GenoCare 1600(GenoCare),which utilizes amplificatio...DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years.In this study,we developed a single-molecule desktop sequencer,GenoCare 1600(GenoCare),which utilizes amplification-free library preparation and two-color sequencing-by-synthesis chemistry,making it more user-friendly compared with previous single-molecule sequencing platforms for clinical use.Using the GenoCare platform,we sequenced an Escherichia coli standard sample and achieved a consensus accuracy exceeding 99.99%.We also evaluated the sequencing performance of this platform in microbial mixtures and coronavirus disease 2019(COVID-19)samples from throat swabs.Our findings indicate that the GenoCare platform allows for microbial quantitation,sensitive identification of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus,and accurate detection of virus mutations,as confirmed by Sanger sequencing,demonstrating its remarkable potential in clinical application.展开更多
Fibrous porous ceramics are attractive for use as thermal insulation materials.However,the intrinsic brit-tleness of rigid materials has remained challenging and severely restricts their applications.Here,we demonstra...Fibrous porous ceramics are attractive for use as thermal insulation materials.However,the intrinsic brit-tleness of rigid materials has remained challenging and severely restricts their applications.Here,we demonstrated a facile method for fabricating elastic fibrous porous ceramics(EFPCs)with high com-pressive strength and low thermal conductivity through ordinary press filtration and subsequent heat treatment.The quasi-layered structure and the well-bonded bridging fibers between layers are the key points for the elasticity of EFPCs.The advanced EFPCs exhibited low density(∼0.126 g cm^(−3)),high com-pressive stress(∼0.356 MPa),and low thermal conductivity(∼0.026 W m^(−1) K^(−1)).Compared with rigid porous fibrous materials,the EFPCs had deformability and excellent shape recovery.In contrast to flexible materials,the EFPCs possessed high compressive stress,thus endowing them with good resistance to de-formation.The emergence of this fascinating material may provide new insights for candidate materials in thermal insulation and other fields.展开更多
文摘DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years.In this study,we developed a single-molecule desktop sequencer,GenoCare 1600(GenoCare),which utilizes amplification-free library preparation and two-color sequencing-by-synthesis chemistry,making it more user-friendly compared with previous single-molecule sequencing platforms for clinical use.Using the GenoCare platform,we sequenced an Escherichia coli standard sample and achieved a consensus accuracy exceeding 99.99%.We also evaluated the sequencing performance of this platform in microbial mixtures and coronavirus disease 2019(COVID-19)samples from throat swabs.Our findings indicate that the GenoCare platform allows for microbial quantitation,sensitive identification of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)virus,and accurate detection of virus mutations,as confirmed by Sanger sequencing,demonstrating its remarkable potential in clinical application.
基金financially supported by the National Key Research and Development of China (No.2021YFB3400200)the National Natural Science Foundation of China (Nos.12090031 and 11602125)the Beijing Institute of Technology Research Fund Program for Young Scholars.
文摘Fibrous porous ceramics are attractive for use as thermal insulation materials.However,the intrinsic brit-tleness of rigid materials has remained challenging and severely restricts their applications.Here,we demonstrated a facile method for fabricating elastic fibrous porous ceramics(EFPCs)with high com-pressive strength and low thermal conductivity through ordinary press filtration and subsequent heat treatment.The quasi-layered structure and the well-bonded bridging fibers between layers are the key points for the elasticity of EFPCs.The advanced EFPCs exhibited low density(∼0.126 g cm^(−3)),high com-pressive stress(∼0.356 MPa),and low thermal conductivity(∼0.026 W m^(−1) K^(−1)).Compared with rigid porous fibrous materials,the EFPCs had deformability and excellent shape recovery.In contrast to flexible materials,the EFPCs possessed high compressive stress,thus endowing them with good resistance to de-formation.The emergence of this fascinating material may provide new insights for candidate materials in thermal insulation and other fields.
