Dear Editor,This letter proposes to integrate dendritic learnable network architecture with Vision Transformer to improve the accuracy of image recognition.In this study,based on the theory of dendritic neurons in neu...Dear Editor,This letter proposes to integrate dendritic learnable network architecture with Vision Transformer to improve the accuracy of image recognition.In this study,based on the theory of dendritic neurons in neuroscience,we design a network that is more practical for engineering to classify visual features.Based on this,we propose a dendritic learning-incorporated vision Transformer(DVT),which out-performs other state-of-the-art methods on three image recognition benchmarks.展开更多
Accurately predicting fluid forces acting on the sur-face of a structure is crucial in engineering design.However,this task becomes particularly challenging in turbulent flow,due to the complex and irregular changes i...Accurately predicting fluid forces acting on the sur-face of a structure is crucial in engineering design.However,this task becomes particularly challenging in turbulent flow,due to the complex and irregular changes in the flow field.In this study,we propose a novel deep learning method,named mapping net-work-coordinated stacked gated recurrent units(MSU),for pre-dicting pressure on a circular cylinder from velocity data.Specifi-cally,our coordinated learning strategy is designed to extract the most critical velocity point for prediction,a process that has not been explored before.In our experiments,MSU extracts one point from a velocity field containing 121 points and utilizes this point to accurately predict 100 pressure points on the cylinder.This method significantly reduces the workload of data measure-ment in practical engineering applications.Our experimental results demonstrate that MSU predictions are highly similar to the real turbulent data in both spatio-temporal and individual aspects.Furthermore,the comparison results show that MSU predicts more precise results,even outperforming models that use all velocity field points.Compared with state-of-the-art methods,MSU has an average improvement of more than 45%in various indicators such as root mean square error(RMSE).Through comprehensive and authoritative physical verification,we estab-lished that MSU’s prediction results closely align with pressure field data obtained in real turbulence fields.This confirmation underscores the considerable potential of MSU for practical applications in real engineering scenarios.The code is available at https://github.com/zhangzm0128/MSU.展开更多
Dear Editor,This letter presents a novel segmentation approach that leverages dendritic neurons to tackle the challenges of medical imaging segmentation.In this study,we enhance the segmentation accuracy based on a Se...Dear Editor,This letter presents a novel segmentation approach that leverages dendritic neurons to tackle the challenges of medical imaging segmentation.In this study,we enhance the segmentation accuracy based on a SegNet variant including an encoder-decoder structure,an upsampling index,and a deep supervision method.Furthermore,we introduce a dendritic neuron-based convolutional block to enable nonlinear feature mapping,thereby further improving the effectiveness of our approach.展开更多
Human activities and industrialization have significantly increased soil nutrients,such as nitrogen(N)and phos-phorus(P),profoundly impacting the composition and structure of plant community,as well as the ecosystem fu...Human activities and industrialization have significantly increased soil nutrients,such as nitrogen(N)and phos-phorus(P),profoundly impacting the composition and structure of plant community,as well as the ecosystem functions,especially in nutrient-limited ecosystems.However,as the key propagule pool of perennial grasslands,how belowground bud bank and its relationship with aboveground vegetation respond to short-term changes in soil nutrients was still unclear.In this study,we conducted a short-term(2021–2022)soil fertilization experiment with N addition(10 g N m^(-2) yr^(-1))and P addition(5 g N m^(-2) yr^(-1))in the meadow steppe of Inner Mongolia,China,to explore the responses of belowground bud bank,aboveground shoot population and their relationships(represented by the ratio of bud to shoot density-meristem limitation index(MLI))for the whole community and three plant functional groups(perennial rhizomatous grasses-PR,perennial bunchgrasses-PB,and perennial forbs-PF)to nutrient addition.The short-term nutrient addition had no significant influences on belowground bud density,aboveground shoot density,and MLI of the whole plant community.Plant functional groups showed different responses to soil fertilization.Specifically,N addition significantly increased the bud density and shoot density of PR,especially in combination with P addition.N addition reduced the shoot density of PF but had no influence on its bud density and MLI.Nutrient addition had significant effects on the three indicators of PB.Our study indicates that the belowground bud bank and its relationship with aboveground vegetation in temperate meadow steppe are insensitive to short-term soil fertilization,but plant functional groups exhibit specific responses in terms of population regeneration,which implies that plant community composition and ecosystem functions will be changed under the ongoing global change.展开更多
Wind energy has been widely applied in power generation to alleviate climate problems.The wind turbine layout of a wind farm is a primary factor of impacting power conversion efficiency due to the wake effect that red...Wind energy has been widely applied in power generation to alleviate climate problems.The wind turbine layout of a wind farm is a primary factor of impacting power conversion efficiency due to the wake effect that reduces the power outputs of wind turbines located in downstream.Wind farm layout optimization(WFLO)aims to reduce the wake effect for maximizing the power outputs of the wind farm.Nevertheless,the wake effect among wind turbines increases significantly as the number of wind turbines increases in the wind farm,which severely affect power conversion efficiency.Conventional heuristic algorithms suffer from issues of low solution quality and local optimum for large-scale WFLO under complex wind scenarios.Thus,a chaotic local search-based genetic learning particle swarm optimizer(CGPSO)is proposed to optimize large-scale WFLO problems.CGPSO is tested on four larger-scale wind farms under four complex wind scenarios and compares with eight state-of-the-art algorithms.The experiment results indicate that CGPSO significantly outperforms its competitors in terms of performance,stability,and robustness.To be specific,a success and failure memories-based selection is proposed to choose a chaotic map for chaotic search local.It improves the solution quality.The parameter and search pattern of chaotic local search are also analyzed for WFLO problems.展开更多
In arid and semi-arid sand dune ecosystems,belowground bud bank plays an important role in population regeneration and vegetation restoration.However,the responses of belowground bud bank size and composition to sand ...In arid and semi-arid sand dune ecosystems,belowground bud bank plays an important role in population regeneration and vegetation restoration.However,the responses of belowground bud bank size and composition to sand burial and its induced changes in soil environmental factors have been rarely studied.In arid sand dunes of Northwestern China,we investigated belowground bud bank size and composition of the typical rhizomatous psammophyte Psammochloa villosa as well as three key soil environmental factors(soil moisture,total carbon and total nitrogen)under different depths of sand burial.Total buds and rhizome buds increased significantly with increasing burial depth,whereas tiller buds first increased and then decreased,with a peak value at the depth of 20-30 cm.Soil moisture increased significantly with sand burial depth,and was positively correlated with the number of all buds and rhizome buds.Soil total carbon concentration first increased and then decreased with sand burial depth,and total nitrogen concentration was significantly lower under deep sand burial than those at shallow depths,and only the number of tiller buds was positively correlated with soil total nitrogen concentration.These results indicate that soil moisture rather than soil nutrient might regulate the belowground bud bank of P.villosa,and that clonal psammophytes could regulate their belowground bud bank in response to sand burial and the most important environmental stress(i.e.,soil moisture).These responses,as the key adaptive strategy,may ensure clonal plant population regeneration and vegetation restoration in arid sand dunes.展开更多
This study proposed an effective plastic deformation technique,rotating backward extrusion(RBE),for producing high performance AZ80 magnesium alloy cup-shaped pieces.The RBE process was carried on the Gleeble-3500 tes...This study proposed an effective plastic deformation technique,rotating backward extrusion(RBE),for producing high performance AZ80 magnesium alloy cup-shaped pieces.The RBE process was carried on the Gleeble-3500 test machine at 653 K,and the conventional backward extrusion(CBE)was also conducted for comparison.A detailed microstructure analysis was performed using the optical microscopy(OM)and electron back-scatter diffraction(EBSD).The results shown that the equivalent strain and deformation uniformity of the cup pieces could be substantially increased by the RBE process compared with the CBE process.Furthermore,the RBE process could significantly improve the grain refining capacity and the proportion of dynamic recrystallization(DRX),of which the maximum reduction of grain size was 88.60%,and the maximum increase of DRX proportion was 55.30%in the cup bottom.The main deformation mechanism of the RBE process was the discontinuous DRX(DDRX),while the continuous DRX(CDRX)was also occurred in the cup transition.Compared with the CBE sample,the texture of the cup bottom was weakened for the RBE sample.The microhardness value of the RBE sample was higher than that of the CBE sample,which can be attributed to the grain refinement strengthening.展开更多
There has been growing attention to intraspecific variation in trait-based plant ecology. However, studies on these changes across ontogenetic stages and the potential trade-offs with interspecific traits along enviro...There has been growing attention to intraspecific variation in trait-based plant ecology. However, studies on these changes across ontogenetic stages and the potential trade-offs with interspecific traits along environmental gradients are rare. In this study, we measured six wood and bark traits of 1030 trees of six species(Castanopsis nigrescens; C. carlesii; Lithocarpus polystachyus; L. synbalanos; Ormosia glaberrima; O. pachycarpa) from a10-ha plot in a subtropical forest. Mean intraspecific variation in bark thickness and bark percentage to DBH was more than twice that for wood density and bark density.Bark thickness and bark percentage showed a consistent trend with increasing tree size. Small-tree traits were more variable than the same traits in larger trees. Altitude,convexity and soil nutrients explained the majority of the variations in the six traits, while sibling species had similar relationships between traits and environmental variables.Trees with dense wood and thin bark were usually found on steep slopes at lower altitudes. Our findings show intraspecific trait variability has different spatial patterns compared with interspecific variabilities along an environmental gradient.展开更多
Photocatalytic water splitting and carbon dioxide photoreduction are considered eff ective strategies for alleviating the energy crisis and environmental pollution.Polynuclear metal-oxo clusters possess excellent elec...Photocatalytic water splitting and carbon dioxide photoreduction are considered eff ective strategies for alleviating the energy crisis and environmental pollution.Polynuclear metal-oxo clusters possess excellent electron storage/release ability and unique catalytic properties via intermetallic synergy,which enables them with great potential in environmentally friendly photosynthesis.Importantly,metal-oxo clusters with precise structure can not only act as high-effi ciency catalysts but also provide well-defi ned structural models for exploring structure-activity relationships.In this review,we systematically sum-marize recent progress in the catalytic application of polynuclear metal-oxo clusters,including polyoxometalate clusters,low-cost transition metal clusters,and metal-oxo-cluster-based metal-organic frameworks for water splitting and CO_(2)reduction.Furthermore,we discuss the challenges and solutions to the problems of polynuclear metal-oxo clusters in photocatalysis.展开更多
Gibberella ear rot(GER)caused by Fusarium graminearum(teleomorph Gibberella zeae)is a common maize disease that not only severely reduces grain yield but also contaminates maize grain with mycotoxins.We investigated t...Gibberella ear rot(GER)caused by Fusarium graminearum(teleomorph Gibberella zeae)is a common maize disease that not only severely reduces grain yield but also contaminates maize grain with mycotoxins.We investigated the molecular mechanism underlying the host defense responses against pathogen infection using comparative transcriptomic analysis.We injected F.graminearum spore suspensions into plants of resistant(IBM-81)and a susceptible(IBM-85)maize inbred line after pollination and performed RNA-seq 48,72,and 96 h after inoculation.Respectively 487 and 410 differentially expressed genes(DEGs)were induced in the resistant and susceptible lines across three time points,indicating that a stronger defense response was activated in the resistant than in the susceptible line.Among them,198 genes commonly induced in the two lines were subjected to pathway analysis,revealing that most of the DEGs were closely associated with defense and a wide range of metabolic activities.DEGs associated with pathogenesis-related protein 1(PR1)and regulation of salicylic acid were significantly enriched during F.graminearum infection,suggesting that these DEGs play dominant roles in maize resistance to GER.Our results provide a resource for future gene discovery and facilitate elucidation of the complex defense mechanisms involved in resistance to GER.展开更多
Cerium oxide nanoparticles(CeO_(2) NPs)are likely to have dramatic impacts on plant performances,yet the effects of CeO_(2) NPs on seed germination and seedling growth have not been fully explored.In this study,the se...Cerium oxide nanoparticles(CeO_(2) NPs)are likely to have dramatic impacts on plant performances,yet the effects of CeO_(2) NPs on seed germination and seedling growth have not been fully explored.In this study,the seed germination and seedling growth of subshrub species Vitex negundo under different concentrations of CeO_(2) NPs(low-1 mg/L,medium-100 mg/L,high-500 mg/L)have been discussed.Results showed that:(1)The seed germination rate reduces by 11.25%and 2.5%under the low and medium concentrations of CeO_(2) NPs,respectively,but increased by 7.08%under the high concentration;(2)CeO_(2) NPs had significant effects on the growth traits(root length,shoot height and biomass)of seedlings,being the highest under the medium concentration and the lowest under the highest concentration;(3)The superoxide dismutase activity was the maximum(355.91 U/g),but the protein concentration was the minimum(3.85 ug/mL)under the high concentration of CeO_(2) NPs.Our results indicated that the effects of CeO_(2) NPs on seed germination and seedling growth are concentration-dependency,i.e.,low and medium concentrations inhibited while high concentration promoted seed germination,however,seedling growth showed opposite responses.Therefore,appropriate CeO_(2) NPs concentrations are beneficial to the seed germination and seedling growth of Vitex negundo and improve the physiological performance of seedlings and enhance their adaptability to environmental adversity.展开更多
[Objective] This study was conducted to screen the best variety and optimum planting density for constructing early-bearing high-yielding model plantation of Camellia oleifera. [Methed] This survey was carried out in ...[Objective] This study was conducted to screen the best variety and optimum planting density for constructing early-bearing high-yielding model plantation of Camellia oleifera. [Methed] This survey was carried out in two demonstration bases, one for investigating the relationship between different cultivated varieties with growth and production, the other for investigating the relationship between different planting densities with growth and production. [Result] For different cultivated varieties, Cenruan 2 showed tree height, ground diameter and crown product significantly higher than other two varieties, besides, the per unit area fruit yield of Cenruan 2 was also significantly higher than other two varieties. From flower bud differentiation,the best variety was Changlin 4, which showed number of differentiated flower buds significantly higher than Changlin 27, but there were no significant differences between Cenruan 2 with Changlin 4 and Changlin 27. For different planting densities, plant height and crown product at planting density of 1.5 m×2 m were significantly higher than those of other two planting densities, but from ground diam- eter, the best planting density was 1.5 m×1.0 m, with a ground diameter significantly higher than other two planting densities. From per unit area fruit yield, the best planting density was 1.5 m×2.0 m, under which early plant growth and fruit production were better. Therefore, the best planting density is 2.0 m ×3.0 m when constructing early-bearing high-yielding model plantations. For variety Cenruan 3, it's fruit production is high in the early pedod, and the best planting density is 1.5 m× 2.0 m or 1.0 m×1.5 m when constructing early-bearing high-yielding model plantations, and after 4-6 years when it put into production, the plants could be transplanted.展开更多
Given the important roles of miRNAs in post-transcriptional gene regulation, identification of differentially expressed miRNAs will facilitate the elucidation of molecular mechanisms underlying kernel development. In ...Given the important roles of miRNAs in post-transcriptional gene regulation, identification of differentially expressed miRNAs will facilitate the elucidation of molecular mechanisms underlying kernel development. In this study, we constructed a small RNA library to comprehensively represent the full complement of individual small RNAs and to characterize miRNA expression profiles in pooled ears of maize(Zea mays L.) at 10, 15,20, 22, 25 and 30 days after pollination(DAP). At least 21 miRNAs were differentially expressed. The differential expression of three of these miRNAs, i.e., miR528a, miR167a and miR160b, at each stage was verified by qRT-PCR. The results indicated that these miRNAs might be involved in kernel development. In addition, the predicted functions of target genes indicated that most of the target genes are involved in signal transduction and cell communication pathways, particularly the auxin signaling pathway. The expression of candidate germination-associated miRNAs was analyzed by hybridization to a maize genome microarray, and revealed differential expression of genes involved in plant hormone signaling pathways. This finding suggests that phytohormones play a critical role in the development of maize kernels. We found that in combination with other miRNAs, miR528a regulated a putative laccase, a Ring-H2 zinc finger protein and a MADS box-like protein, whereas miR167a and miR160b regulated multiple target genes,including ARF(auxin response factor), a member of the B3 transcription factor family. All three miRNAs are important for ear germination, development and physiology. The small RNA transcriptomes and mRNA obtained in this study will help us gain a betterunderstanding of the expression and function of small RNAs in the development of maize kernel.展开更多
The leaching experiments of soils contaminated by heavy metal Pb or Cd were conducted with four kinds of environmental materials, polymer absorbent material (PAM), coal-based nutrient (CBN), mineral adsorption mat...The leaching experiments of soils contaminated by heavy metal Pb or Cd were conducted with four kinds of environmental materials, polymer absorbent material (PAM), coal-based nutrient (CBN), mineral adsorption material (MAM) and mineral chemical material (MCM) to explore Pb and Cd solidification effects. Then the Fourier Transform Infrared Spectrometer (FTIR) analysis was used to explain the solidification mechanism of the effective environmental materials, and the Scanning Electron Microscope (SEM) test was applied to demonstrate their solidifi- cation morphology. The results showed that MCM was the best material for Pb solidification, and Pb concentration in the leachate accounted for 68.6% of that in CK; MAM was the best material for Cd solidification, and the Cd concentration accounted for 74.6% of that in CK. The FTIR analysis showed the functional groups, such as C-OH in MCM and COOH in MAM, could explain the adsorption mechanism by ion exchange and chemical bond force. The SEM images indicated there were obvious adsorption morphological changes in MCM and MAM after adsorbing Pb and Cd respectively.展开更多
This study was conducted to investigate the growth and development laws of different mink varieties. The weights of six mink varieties were determined at different growth and development stages, respectively. The grow...This study was conducted to investigate the growth and development laws of different mink varieties. The weights of six mink varieties were determined at different growth and development stages, respectively. The growth curves were drawn, and the differences in 150-day-old weight were analyzed. The results showed that the weight of each mink variety grew rapidly after weaning to adult weight; the weight of sliver blue mink was significantly higher than those of imported regal white mink and imported palomino mink; and there were no significant differences between other varieties.展开更多
The authors cloned and identified a new maize serine carboxypeptidase gene named ZmSCP from R15 inbred lines seedlings which were induced by Rhizoctonia solani AGI-IA. ZmSCP encodes a 332 amino acid protein with a pre...The authors cloned and identified a new maize serine carboxypeptidase gene named ZmSCP from R15 inbred lines seedlings which were induced by Rhizoctonia solani AGI-IA. ZmSCP encodes a 332 amino acid protein with a predicted molecular mass of 36.5 kDa and pI of 4.75. Phylogenetic analysis revealed that ZmSCP showed closer kinship with Oryza sativa and sorghum, which belong to the same evolutionary branch. Amino acid sequence analysis revealed that there are four types of amino acids in ZmSCP, the percentages of them are 43.1%, 26.9%, 13.9% and 13.1%. The authors subsequently purified the recombinant protein which expressed in Escherichia coli BL21 and analyzed its antimicrobial activities in vitro. Results showed that the recombinant protein inhibited hyphal growth of Rhizoctonia solani. The study suggests that the expression of ZmSCP is closely related to maize sheath blight resistance caused by Rhizoctonia solani. Further, the antifungal activity showed that ZmSCP may play at role in the disease resistance response.展开更多
Strategies to improve T cell therapy efficacy in solid tumors such as hepatocellular carcinoma(HCC)are urgently needed.The common cytokine receptorγchain(γc)family cytokines such as IL-2,IL-7,IL-15 and IL-21 play fu...Strategies to improve T cell therapy efficacy in solid tumors such as hepatocellular carcinoma(HCC)are urgently needed.The common cytokine receptorγchain(γc)family cytokines such as IL-2,IL-7,IL-15 and IL-21 play fundamental roles in T cell development,differentiation and effector phases.This study aims to determine the combination effects of IL-21 in T cell therapy against HCC and investigate optimized strategies to utilize the effect of IL-21 signal in T cell therapy.The antitumor function of AFP-specific T cell receptor-engineered T cells(TCR-T)was augmented by exogenous IL-21 in vitro and in vivo.IL-21 enhanced proliferation capacity,promoted memory differentiation,downregulated PD-1 expression and alleviated apoptosis in TCR-T after activation.A novel engineered IL-21 receptor was established,and TCR-T armed with the novel engineered IL-21 receptors(IL-21R-TCR-T)showed upregulated phosphorylated STAT3 expression without exogenous IL-21 ligand.IL-21R-TCR-T showed better proliferation upon activation and superior antitumor function in vitro and in vivo.IL-21R-TCR-T exhibited a less differentiated,exhausted and apoptotic phenotype than conventional TCR-T upon repetitive tumor antigen stimulation.The novel IL-21 receptor in our study programs powerful TCR-T and can avoid side effects induced by IL-21 systemic utilization.The novel IL-21 receptor creates new opportunities for next-generation TCR-T against HCC.展开更多
基金partially supported by the Japan Society for the Promotion of Science(JSPS)KAKENHI(JP22H03643)Japan Science and Technology Agency(JST)Support for Pioneering Research Initiated by the Next Generation(SPRING)(JPMJSP2145)JST through the Establishment of University Fellowships towards the Creation of Science Technology Innovation(JPMJFS2115)。
文摘Dear Editor,This letter proposes to integrate dendritic learnable network architecture with Vision Transformer to improve the accuracy of image recognition.In this study,based on the theory of dendritic neurons in neuroscience,we design a network that is more practical for engineering to classify visual features.Based on this,we propose a dendritic learning-incorporated vision Transformer(DVT),which out-performs other state-of-the-art methods on three image recognition benchmarks.
基金supported by the Japan Society for the Promotion of Science(JSPS)KAKENHI(JP22H03643)Japan Science and Technology Agency(JST)Support for Pioneering Research Initiated by the Next Generation(SPRING)(JPMJSP2145)+2 种基金JST Through the Establishment of University Fellowships Towards the Creation of Science Technology Innovation(JPMJFS2115)the National Natural Science Foundation of China(52078382)the State Key Laboratory of Disaster Reduction in Civil Engineering(CE19-A-01)。
文摘Accurately predicting fluid forces acting on the sur-face of a structure is crucial in engineering design.However,this task becomes particularly challenging in turbulent flow,due to the complex and irregular changes in the flow field.In this study,we propose a novel deep learning method,named mapping net-work-coordinated stacked gated recurrent units(MSU),for pre-dicting pressure on a circular cylinder from velocity data.Specifi-cally,our coordinated learning strategy is designed to extract the most critical velocity point for prediction,a process that has not been explored before.In our experiments,MSU extracts one point from a velocity field containing 121 points and utilizes this point to accurately predict 100 pressure points on the cylinder.This method significantly reduces the workload of data measure-ment in practical engineering applications.Our experimental results demonstrate that MSU predictions are highly similar to the real turbulent data in both spatio-temporal and individual aspects.Furthermore,the comparison results show that MSU predicts more precise results,even outperforming models that use all velocity field points.Compared with state-of-the-art methods,MSU has an average improvement of more than 45%in various indicators such as root mean square error(RMSE).Through comprehensive and authoritative physical verification,we estab-lished that MSU’s prediction results closely align with pressure field data obtained in real turbulence fields.This confirmation underscores the considerable potential of MSU for practical applications in real engineering scenarios.The code is available at https://github.com/zhangzm0128/MSU.
基金partially supported by the Japan Society for the Promotion of Science(JSPS)KAKENHI(JP22H03643)Japan Science and Technology Agency(JST)Support for Pioneering Research Initiated by the Next Generation(SPRING)(JPMJSP2145)JST through the Establishment of University Fellowships Towards the Creation of Science Technology Innovation(JPMJFS2115)。
文摘Dear Editor,This letter presents a novel segmentation approach that leverages dendritic neurons to tackle the challenges of medical imaging segmentation.In this study,we enhance the segmentation accuracy based on a SegNet variant including an encoder-decoder structure,an upsampling index,and a deep supervision method.Furthermore,we introduce a dendritic neuron-based convolutional block to enable nonlinear feature mapping,thereby further improving the effectiveness of our approach.
基金support from the National Natural Science Foundation of China(41877542).
文摘Human activities and industrialization have significantly increased soil nutrients,such as nitrogen(N)and phos-phorus(P),profoundly impacting the composition and structure of plant community,as well as the ecosystem functions,especially in nutrient-limited ecosystems.However,as the key propagule pool of perennial grasslands,how belowground bud bank and its relationship with aboveground vegetation respond to short-term changes in soil nutrients was still unclear.In this study,we conducted a short-term(2021–2022)soil fertilization experiment with N addition(10 g N m^(-2) yr^(-1))and P addition(5 g N m^(-2) yr^(-1))in the meadow steppe of Inner Mongolia,China,to explore the responses of belowground bud bank,aboveground shoot population and their relationships(represented by the ratio of bud to shoot density-meristem limitation index(MLI))for the whole community and three plant functional groups(perennial rhizomatous grasses-PR,perennial bunchgrasses-PB,and perennial forbs-PF)to nutrient addition.The short-term nutrient addition had no significant influences on belowground bud density,aboveground shoot density,and MLI of the whole plant community.Plant functional groups showed different responses to soil fertilization.Specifically,N addition significantly increased the bud density and shoot density of PR,especially in combination with P addition.N addition reduced the shoot density of PF but had no influence on its bud density and MLI.Nutrient addition had significant effects on the three indicators of PB.Our study indicates that the belowground bud bank and its relationship with aboveground vegetation in temperate meadow steppe are insensitive to short-term soil fertilization,but plant functional groups exhibit specific responses in terms of population regeneration,which implies that plant community composition and ecosystem functions will be changed under the ongoing global change.
基金partially supported by the Japan Society for the Promotion of Science(JSPS)KAKENHI(JP22H03643)Japan Science and Technology Agency(JST)Support for Pioneering Research Initiated by the Next Generation(SPRING)(JPMJSP2145)JST through the Establishment of University Fellowships towards the Creation of Science Technology Innovation(JPMJFS2115)。
文摘Wind energy has been widely applied in power generation to alleviate climate problems.The wind turbine layout of a wind farm is a primary factor of impacting power conversion efficiency due to the wake effect that reduces the power outputs of wind turbines located in downstream.Wind farm layout optimization(WFLO)aims to reduce the wake effect for maximizing the power outputs of the wind farm.Nevertheless,the wake effect among wind turbines increases significantly as the number of wind turbines increases in the wind farm,which severely affect power conversion efficiency.Conventional heuristic algorithms suffer from issues of low solution quality and local optimum for large-scale WFLO under complex wind scenarios.Thus,a chaotic local search-based genetic learning particle swarm optimizer(CGPSO)is proposed to optimize large-scale WFLO problems.CGPSO is tested on four larger-scale wind farms under four complex wind scenarios and compares with eight state-of-the-art algorithms.The experiment results indicate that CGPSO significantly outperforms its competitors in terms of performance,stability,and robustness.To be specific,a success and failure memories-based selection is proposed to choose a chaotic map for chaotic search local.It improves the solution quality.The parameter and search pattern of chaotic local search are also analyzed for WFLO problems.
基金This work was financially supported by the National Natural Science Foundation of China(41877542,41907411).
文摘In arid and semi-arid sand dune ecosystems,belowground bud bank plays an important role in population regeneration and vegetation restoration.However,the responses of belowground bud bank size and composition to sand burial and its induced changes in soil environmental factors have been rarely studied.In arid sand dunes of Northwestern China,we investigated belowground bud bank size and composition of the typical rhizomatous psammophyte Psammochloa villosa as well as three key soil environmental factors(soil moisture,total carbon and total nitrogen)under different depths of sand burial.Total buds and rhizome buds increased significantly with increasing burial depth,whereas tiller buds first increased and then decreased,with a peak value at the depth of 20-30 cm.Soil moisture increased significantly with sand burial depth,and was positively correlated with the number of all buds and rhizome buds.Soil total carbon concentration first increased and then decreased with sand burial depth,and total nitrogen concentration was significantly lower under deep sand burial than those at shallow depths,and only the number of tiller buds was positively correlated with soil total nitrogen concentration.These results indicate that soil moisture rather than soil nutrient might regulate the belowground bud bank of P.villosa,and that clonal psammophytes could regulate their belowground bud bank in response to sand burial and the most important environmental stress(i.e.,soil moisture).These responses,as the key adaptive strategy,may ensure clonal plant population regeneration and vegetation restoration in arid sand dunes.
基金financially supported by the National Natural Science Foundation of China(No.51775520)the National Key Research and Development Program(No.2016YFB0301103-3)+1 种基金the Key R&D program of Shanxi Province(International Cooperation)(No.201903D421036)the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(No.2018002)。
文摘This study proposed an effective plastic deformation technique,rotating backward extrusion(RBE),for producing high performance AZ80 magnesium alloy cup-shaped pieces.The RBE process was carried on the Gleeble-3500 test machine at 653 K,and the conventional backward extrusion(CBE)was also conducted for comparison.A detailed microstructure analysis was performed using the optical microscopy(OM)and electron back-scatter diffraction(EBSD).The results shown that the equivalent strain and deformation uniformity of the cup pieces could be substantially increased by the RBE process compared with the CBE process.Furthermore,the RBE process could significantly improve the grain refining capacity and the proportion of dynamic recrystallization(DRX),of which the maximum reduction of grain size was 88.60%,and the maximum increase of DRX proportion was 55.30%in the cup bottom.The main deformation mechanism of the RBE process was the discontinuous DRX(DDRX),while the continuous DRX(CDRX)was also occurred in the cup transition.Compared with the CBE sample,the texture of the cup bottom was weakened for the RBE sample.The microhardness value of the RBE sample was higher than that of the CBE sample,which can be attributed to the grain refinement strengthening.
基金funded by the National Natural Science Foundation of China(Key Projects 31230013,31361140363)the Zhang-Hongda Science Foundation at Sun Yat-sen University
文摘There has been growing attention to intraspecific variation in trait-based plant ecology. However, studies on these changes across ontogenetic stages and the potential trade-offs with interspecific traits along environmental gradients are rare. In this study, we measured six wood and bark traits of 1030 trees of six species(Castanopsis nigrescens; C. carlesii; Lithocarpus polystachyus; L. synbalanos; Ormosia glaberrima; O. pachycarpa) from a10-ha plot in a subtropical forest. Mean intraspecific variation in bark thickness and bark percentage to DBH was more than twice that for wood density and bark density.Bark thickness and bark percentage showed a consistent trend with increasing tree size. Small-tree traits were more variable than the same traits in larger trees. Altitude,convexity and soil nutrients explained the majority of the variations in the six traits, while sibling species had similar relationships between traits and environmental variables.Trees with dense wood and thin bark were usually found on steep slopes at lower altitudes. Our findings show intraspecific trait variability has different spatial patterns compared with interspecific variabilities along an environmental gradient.
基金supported by National Natural Science Foundationof China(Grant No.21671113)the Science and Technology of Henan province in 2018(No.182102310873)+2 种基金2019 Special Project of Nanyang Normal University(Nos.2019ZX009 and 2019QN011)Project of Young Backbone Teachers in Colleges and Universities of Henan Province(No.2020GGJS180)2019 Henan Higher Education Teaching Reform Research and Practice Project(No.2019SJGLX093Y).
文摘Photocatalytic water splitting and carbon dioxide photoreduction are considered eff ective strategies for alleviating the energy crisis and environmental pollution.Polynuclear metal-oxo clusters possess excellent electron storage/release ability and unique catalytic properties via intermetallic synergy,which enables them with great potential in environmentally friendly photosynthesis.Importantly,metal-oxo clusters with precise structure can not only act as high-effi ciency catalysts but also provide well-defi ned structural models for exploring structure-activity relationships.In this review,we systematically sum-marize recent progress in the catalytic application of polynuclear metal-oxo clusters,including polyoxometalate clusters,low-cost transition metal clusters,and metal-oxo-cluster-based metal-organic frameworks for water splitting and CO_(2)reduction.Furthermore,we discuss the challenges and solutions to the problems of polynuclear metal-oxo clusters in photocatalysis.
基金supported by the National Natural Science Foundation of China(31471513,31601316)Innovation Training Program of Sichuan Agricultural University(201710626050)
文摘Gibberella ear rot(GER)caused by Fusarium graminearum(teleomorph Gibberella zeae)is a common maize disease that not only severely reduces grain yield but also contaminates maize grain with mycotoxins.We investigated the molecular mechanism underlying the host defense responses against pathogen infection using comparative transcriptomic analysis.We injected F.graminearum spore suspensions into plants of resistant(IBM-81)and a susceptible(IBM-85)maize inbred line after pollination and performed RNA-seq 48,72,and 96 h after inoculation.Respectively 487 and 410 differentially expressed genes(DEGs)were induced in the resistant and susceptible lines across three time points,indicating that a stronger defense response was activated in the resistant than in the susceptible line.Among them,198 genes commonly induced in the two lines were subjected to pathway analysis,revealing that most of the DEGs were closely associated with defense and a wide range of metabolic activities.DEGs associated with pathogenesis-related protein 1(PR1)and regulation of salicylic acid were significantly enriched during F.graminearum infection,suggesting that these DEGs play dominant roles in maize resistance to GER.Our results provide a resource for future gene discovery and facilitate elucidation of the complex defense mechanisms involved in resistance to GER.
基金This study was supported by the Science and Technology Innovation Foundation of Henan Agricultural University(30601458)the Scientific Research Foundation for the Doctor of Henan Agriculture University(30500848).
文摘Cerium oxide nanoparticles(CeO_(2) NPs)are likely to have dramatic impacts on plant performances,yet the effects of CeO_(2) NPs on seed germination and seedling growth have not been fully explored.In this study,the seed germination and seedling growth of subshrub species Vitex negundo under different concentrations of CeO_(2) NPs(low-1 mg/L,medium-100 mg/L,high-500 mg/L)have been discussed.Results showed that:(1)The seed germination rate reduces by 11.25%and 2.5%under the low and medium concentrations of CeO_(2) NPs,respectively,but increased by 7.08%under the high concentration;(2)CeO_(2) NPs had significant effects on the growth traits(root length,shoot height and biomass)of seedlings,being the highest under the medium concentration and the lowest under the highest concentration;(3)The superoxide dismutase activity was the maximum(355.91 U/g),but the protein concentration was the minimum(3.85 ug/mL)under the high concentration of CeO_(2) NPs.Our results indicated that the effects of CeO_(2) NPs on seed germination and seedling growth are concentration-dependency,i.e.,low and medium concentrations inhibited while high concentration promoted seed germination,however,seedling growth showed opposite responses.Therefore,appropriate CeO_(2) NPs concentrations are beneficial to the seed germination and seedling growth of Vitex negundo and improve the physiological performance of seedlings and enhance their adaptability to environmental adversity.
基金Supported by Scientific Research and Development Planning Project of Guilin City(20130108-6-1)~~
文摘[Objective] This study was conducted to screen the best variety and optimum planting density for constructing early-bearing high-yielding model plantation of Camellia oleifera. [Methed] This survey was carried out in two demonstration bases, one for investigating the relationship between different cultivated varieties with growth and production, the other for investigating the relationship between different planting densities with growth and production. [Result] For different cultivated varieties, Cenruan 2 showed tree height, ground diameter and crown product significantly higher than other two varieties, besides, the per unit area fruit yield of Cenruan 2 was also significantly higher than other two varieties. From flower bud differentiation,the best variety was Changlin 4, which showed number of differentiated flower buds significantly higher than Changlin 27, but there were no significant differences between Cenruan 2 with Changlin 4 and Changlin 27. For different planting densities, plant height and crown product at planting density of 1.5 m×2 m were significantly higher than those of other two planting densities, but from ground diam- eter, the best planting density was 1.5 m×1.0 m, with a ground diameter significantly higher than other two planting densities. From per unit area fruit yield, the best planting density was 1.5 m×2.0 m, under which early plant growth and fruit production were better. Therefore, the best planting density is 2.0 m ×3.0 m when constructing early-bearing high-yielding model plantations. For variety Cenruan 3, it's fruit production is high in the early pedod, and the best planting density is 1.5 m× 2.0 m or 1.0 m×1.5 m when constructing early-bearing high-yielding model plantations, and after 4-6 years when it put into production, the plants could be transplanted.
基金supported by grants from the Educational Commission of Sichuan Province (No. 2006J13-039)the Doctoral Program Foundation of Institutions of Higher Education of China (No. 20095103120002)the National Natural Science Foundation of China (No. 30900901)
文摘Given the important roles of miRNAs in post-transcriptional gene regulation, identification of differentially expressed miRNAs will facilitate the elucidation of molecular mechanisms underlying kernel development. In this study, we constructed a small RNA library to comprehensively represent the full complement of individual small RNAs and to characterize miRNA expression profiles in pooled ears of maize(Zea mays L.) at 10, 15,20, 22, 25 and 30 days after pollination(DAP). At least 21 miRNAs were differentially expressed. The differential expression of three of these miRNAs, i.e., miR528a, miR167a and miR160b, at each stage was verified by qRT-PCR. The results indicated that these miRNAs might be involved in kernel development. In addition, the predicted functions of target genes indicated that most of the target genes are involved in signal transduction and cell communication pathways, particularly the auxin signaling pathway. The expression of candidate germination-associated miRNAs was analyzed by hybridization to a maize genome microarray, and revealed differential expression of genes involved in plant hormone signaling pathways. This finding suggests that phytohormones play a critical role in the development of maize kernels. We found that in combination with other miRNAs, miR528a regulated a putative laccase, a Ring-H2 zinc finger protein and a MADS box-like protein, whereas miR167a and miR160b regulated multiple target genes,including ARF(auxin response factor), a member of the B3 transcription factor family. All three miRNAs are important for ear germination, development and physiology. The small RNA transcriptomes and mRNA obtained in this study will help us gain a betterunderstanding of the expression and function of small RNAs in the development of maize kernel.
基金Supported by the National " Twelfth Five-Year" Plan for Science & Technology Support ( 2011AA100503)
文摘The leaching experiments of soils contaminated by heavy metal Pb or Cd were conducted with four kinds of environmental materials, polymer absorbent material (PAM), coal-based nutrient (CBN), mineral adsorption material (MAM) and mineral chemical material (MCM) to explore Pb and Cd solidification effects. Then the Fourier Transform Infrared Spectrometer (FTIR) analysis was used to explain the solidification mechanism of the effective environmental materials, and the Scanning Electron Microscope (SEM) test was applied to demonstrate their solidifi- cation morphology. The results showed that MCM was the best material for Pb solidification, and Pb concentration in the leachate accounted for 68.6% of that in CK; MAM was the best material for Cd solidification, and the Cd concentration accounted for 74.6% of that in CK. The FTIR analysis showed the functional groups, such as C-OH in MCM and COOH in MAM, could explain the adsorption mechanism by ion exchange and chemical bond force. The SEM images indicated there were obvious adsorption morphological changes in MCM and MAM after adsorbing Pb and Cd respectively.
基金Supported by National Natural Science Foundation of China(31501958)Jilin Department of Science and Technology(20150101113JC)Special Animal Genetic Resource Innovation Team Fund of Chinese Academy of Agricultural Sciences
文摘This study was conducted to investigate the growth and development laws of different mink varieties. The weights of six mink varieties were determined at different growth and development stages, respectively. The growth curves were drawn, and the differences in 150-day-old weight were analyzed. The results showed that the weight of each mink variety grew rapidly after weaning to adult weight; the weight of sliver blue mink was significantly higher than those of imported regal white mink and imported palomino mink; and there were no significant differences between other varieties.
基金Acknowledgments This research was financially supported by the Natural Science Foundation (30900901), the Science and Technology Department Application Foundation of Sichuan province (2006J13-039), and the Agriculture Project of Ministry (2011ZX08003-003).
文摘The authors cloned and identified a new maize serine carboxypeptidase gene named ZmSCP from R15 inbred lines seedlings which were induced by Rhizoctonia solani AGI-IA. ZmSCP encodes a 332 amino acid protein with a predicted molecular mass of 36.5 kDa and pI of 4.75. Phylogenetic analysis revealed that ZmSCP showed closer kinship with Oryza sativa and sorghum, which belong to the same evolutionary branch. Amino acid sequence analysis revealed that there are four types of amino acids in ZmSCP, the percentages of them are 43.1%, 26.9%, 13.9% and 13.1%. The authors subsequently purified the recombinant protein which expressed in Escherichia coli BL21 and analyzed its antimicrobial activities in vitro. Results showed that the recombinant protein inhibited hyphal growth of Rhizoctonia solani. The study suggests that the expression of ZmSCP is closely related to maize sheath blight resistance caused by Rhizoctonia solani. Further, the antifungal activity showed that ZmSCP may play at role in the disease resistance response.
基金The study was sponsored by the National Key Research and Development Program of China(No.2022YFC2303600)the National Natural Science Foundation of China(82073360 and 81802449)+1 种基金the Guangdong Basic and Applied Basic Research Foundation(2023A1515012169,2021A1515220100,2020A1515011313)the Outstanding Youth Development Scheme of Nanfang Hospital,Southern Medical University(2020J004).
文摘Strategies to improve T cell therapy efficacy in solid tumors such as hepatocellular carcinoma(HCC)are urgently needed.The common cytokine receptorγchain(γc)family cytokines such as IL-2,IL-7,IL-15 and IL-21 play fundamental roles in T cell development,differentiation and effector phases.This study aims to determine the combination effects of IL-21 in T cell therapy against HCC and investigate optimized strategies to utilize the effect of IL-21 signal in T cell therapy.The antitumor function of AFP-specific T cell receptor-engineered T cells(TCR-T)was augmented by exogenous IL-21 in vitro and in vivo.IL-21 enhanced proliferation capacity,promoted memory differentiation,downregulated PD-1 expression and alleviated apoptosis in TCR-T after activation.A novel engineered IL-21 receptor was established,and TCR-T armed with the novel engineered IL-21 receptors(IL-21R-TCR-T)showed upregulated phosphorylated STAT3 expression without exogenous IL-21 ligand.IL-21R-TCR-T showed better proliferation upon activation and superior antitumor function in vitro and in vivo.IL-21R-TCR-T exhibited a less differentiated,exhausted and apoptotic phenotype than conventional TCR-T upon repetitive tumor antigen stimulation.The novel IL-21 receptor in our study programs powerful TCR-T and can avoid side effects induced by IL-21 systemic utilization.The novel IL-21 receptor creates new opportunities for next-generation TCR-T against HCC.