Hazelnut is popular for its flavor,and it has also been suggested that hazelnut is beneficial to cardiovascular health because it is rich in oleic acid.Here,we report the first high-quality chromosome-scale genome for...Hazelnut is popular for its flavor,and it has also been suggested that hazelnut is beneficial to cardiovascular health because it is rich in oleic acid.Here,we report the first high-quality chromosome-scale genome for the hazelnut species Corylus mandshurica(2n=22),which has a high concentration of oleic acid in its nuts.The assembled genome is 367.67Mb in length,and the contig N50 is 14.85 Mb.All contigs were assembled into 11 chromosomes,and 28,409 protein-coding genes were annotated.We reconstructed the evolutionary trajectories of the genomes of Betulaceae species and revealed that the 11 chromosomes of the hazelnut genus were derived from the most ancestral karyotype in Betula pendula,which has 14 protochromosomes,by inferring homology among five Betulaceae genomes.We identified 96 candidate genes involved in oleic acid biosynthesis,and 10 showed rapid evolution or positive selection.These findings will help us to understand the mechanisms of lipid synthesis and storage in hazelnuts.Several gene families related to salicylic acid metabolism and stress responses experienced rapid expansion in this hazelnut species,which may have increased its stress tolerance.The reference genome presented here constitutes a valuable resource for molecular breeding and genetic improvement of the important agronomic properties of hazelnut.展开更多
Endoparasitoid wasps introduce venom into their host insects during the egglaying stage.Venom proteins play various roles in the host physiology,development,immunity,and behavior manipulation and regulation.In this st...Endoparasitoid wasps introduce venom into their host insects during the egglaying stage.Venom proteins play various roles in the host physiology,development,immunity,and behavior manipulation and regulation.In this study,we identified a venom protein,MmRhol,a small guanine nucleotide-binding protein derived from ovary in the endoparasitoid wasp Microplitis mediator and found that knockdown of its expression by RNA interference caused down-regulation of vitellogenin and juvenile hormone,egg production,and cocoons formation in the female wasps.We demonstrated that MmRho1 entered the cotton bollworm's(host)hemocytes and suppressed cellular immune responses after parasitism using immunofluorescence staining.Furthermore,wasp MmRhol interacted with the cotton bollworm's actin cytoskeleton rearrangement regulator diaphanous by yeast 2-hybrid and glutathione s-transferase pull-down.In conclusion,this study indicates that MmRho1 plays dual roles in wasp development and the suppression of the host insect cellular immune responses.展开更多
As the major bitter-and astringent-tasting components in tea leaves,catechins play an essential role in determining flavor characteristics and health benefits of tea products.This study analyzed the circadian rhythm e...As the major bitter-and astringent-tasting components in tea leaves,catechins play an essential role in determining flavor characteristics and health benefits of tea products.This study analyzed the circadian rhythm effects on the accumulation pattern of catechins in tender leaves of three tea cultivars,'Huangkui'(HK),'Longjing43'(LJ43)and'Fuding Dabaicha'(FD).There were two major factors involved in the catechin regulation in tea plants:genetic background of the cultivar-specific regulation,and temporal effects of the circadian rhythm.Etiolated leaves of'HK'contained lower levels of catechins than'LJ43'and'FD'.The composition of EGCG in total catechins was significantly higher in'LJ43'leaves(65%)when compared with that in'HK'and'FD'(45%).The accumulation of individual catechins increased during the daytime but decreased overnight,especially over the time period of 22:00−02:00.The correlation analysis between catechins and environmental factors indicated catechin accumulation in tea leaves potentially resulted from a combined regulation of light and temperature.These findings provide new insights into our understanding of circadian rhythm regulation on flavor-important secondary metabolites in tea leaves.展开更多
For this research,electroencephalography(EEG)was analyzed to investigate the perception ability of the brain for moving objects at different speeds.In this experiment,total six kinds of videos regarding license plates...For this research,electroencephalography(EEG)was analyzed to investigate the perception ability of the brain for moving objects at different speeds.In this experiment,total six kinds of videos regarding license plates were created,moving at distinct speed of 0.26 m/s,0.36 m/s,0.46 m/s,0.56 m/s,0.66 m/s,and 0.76 m/s,respectively.In the semantic priming paradigm,the N400 effect was analyzed for each speed.The ERP results demonstrated that the N400 amplitude gradually reduced with increasing speed.At the three lower speeds,N400 was evoked evidently and mainly distributed in the centro-posterior region.At the three higher speeds,no significant N400 effect was found.The results concluded that the perception ability of the brain declined with the acceleration of the object’s moving speed and that the brain recognized the detailed information of the moving object when its speed was lower than 0.46 m/s.展开更多
It is increasingly realized that homoploid hybrid speciation(HHS),which involves no change in chromosome number,is an important mechanism of speciation.HHS will likely increase in frequency as ecological and geographi...It is increasingly realized that homoploid hybrid speciation(HHS),which involves no change in chromosome number,is an important mechanism of speciation.HHS will likely increase in frequency as ecological and geographical barriers between species are continuing to be disrupted by human activities.HHS requires the establishment of reproductive isolation between a hybrid and its parents,but the underlying genes and genetic mechanisms remain largely unknown.In this study,we reveal by integrated approaches that reproductive isolation originates in one homoploid hybrid plant species through the inheritance of altemate alleles at genes that determine parental premating isolation.The parent species of this hybrid species are reproductively isolated by differences in flowering time and survivorship on soils containing high concentrations of iron.We found that the hybrid species inherits alleles of parental isolating major genes related to flowering time from one parent and alleles of major genes related to iron tolerance from the other parent.In this way,it became reproductively isolated from one parent by the difference in flowering time and from the other by habitat adaptation(iron tolerance).These findings and further modeling results suggest that HHS may occur relatively easily via the inheritance of alternate parental premating isolating genes and barriers.展开更多
Peptidoglycan recognition proteins (PGRPs) are a family of innate immune receptors that specifically recognize peptidoglycans (PGNs) on the surface of a number of pathogens. Here, we have identified and characteri...Peptidoglycan recognition proteins (PGRPs) are a family of innate immune receptors that specifically recognize peptidoglycans (PGNs) on the surface of a number of pathogens. Here, we have identified and characterized six PGRPs from endoparasitoid wasp, Microplitis mediator (MmePGRPs). To understand the roles of PGRPs in parasitoid wasps, we analyzed their evolutionary relationship and orthology, expression profiles during different developmental stages, and transcriptional expression following infection with Gram-positive and -negative bacteria and a fungus. MmePGRP-S1 was significantly induced in response to pathogenic infection. This prompted us to evaluate the effects of RNA interference mediated gene specific knockdown ofMmePGRP-S1. The knockdown of MmePGRP-S1 (iMmePGRP-S1) dramatically affected wasps' survival following challenge by Micrococcus luteus, indicating the involvement of this particular PGRP in immune responses against Gram-positive bacteria. This action is likely to be mediated by the Toll pathway, but the mechanism remains to be determined. MmePGRP-S 1 does not play a significant role in anti-fungal immunity as indicated by the survival rate of iMmePGRP-S wasps. This study provides a comprehensive characterization of PGRPs in the economically important hymenopteran species M. mediator.展开更多
Bacterial infection causes enhanced reactive oxygen species (ROS) levels in insects. Oxidation resistance 1 (OXR1) plays an antioxidant role in eukaryotic organisms, including insects. In this report, we demonstra...Bacterial infection causes enhanced reactive oxygen species (ROS) levels in insects. Oxidation resistance 1 (OXR1) plays an antioxidant role in eukaryotic organisms, including insects. In this report, we demonstrated that Pseudomonas aeruginosa and Staphylococcus aureus infection and hydrogen peroxide (H2O2) injection induced the expression of specific transcriptional isoforms of OXR1 in larval silkworms. We further showed that a Jun kinase (JNK) pathway inhibitor, SP600125, down-regulated expression of OXRI during infection, leading to elevated H2O2 levels in the hemolymph, resulting in lower viability of the injected bacteria inside the silkworm larvae. Our study suggests that OXR1 participates in protecting larval silkworms from oxidative stress and bacterial infection through the JNK pathway.展开更多
To analyze the effect of energy conservation policies on energy consumption of residential buildings, the characteristics of energy consumption and indoor thermal comfort were investigated in detail in Tianjin, China,...To analyze the effect of energy conservation policies on energy consumption of residential buildings, the characteristics of energy consumption and indoor thermal comfort were investigated in detail in Tianjin, China, based on official statistical yearbook and field survey data. A comprehensive survey of 305 households indicates that the mean electricity consumption per household is 3215kWh/a, in which annual cooling electricity consumption is 344kWh/a, and the mean natural gas consumption for cooking is 103.2m3/a. Analysis of 3966 households data shows that space heating average intensity of residential buildings designed before 1996 is 133.7kWh/(m^2.a), that of buildings designed between 1996 and 2004 is 117.2 kWh/(m^2.a), and that of buildings designed after 2004 is 105.0kWh/(m^2.a). Apparently, enhancing the performance of envelops is effective in reducing space heating intensity. Furthermore, the results of questionnaires show that 18% of the residents feel slightly warm and hot respectively, while 3% feel slightly cold in winter. Therefore, the electricity consumption in summer will rise for meeting indoor thermal comfort.展开更多
基金the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB31010300)the National Key Research and Development Program of China(2017YFC0505203)the National Natural Science Foundation of China(31590821 and 31900201)。
文摘Hazelnut is popular for its flavor,and it has also been suggested that hazelnut is beneficial to cardiovascular health because it is rich in oleic acid.Here,we report the first high-quality chromosome-scale genome for the hazelnut species Corylus mandshurica(2n=22),which has a high concentration of oleic acid in its nuts.The assembled genome is 367.67Mb in length,and the contig N50 is 14.85 Mb.All contigs were assembled into 11 chromosomes,and 28,409 protein-coding genes were annotated.We reconstructed the evolutionary trajectories of the genomes of Betulaceae species and revealed that the 11 chromosomes of the hazelnut genus were derived from the most ancestral karyotype in Betula pendula,which has 14 protochromosomes,by inferring homology among five Betulaceae genomes.We identified 96 candidate genes involved in oleic acid biosynthesis,and 10 showed rapid evolution or positive selection.These findings will help us to understand the mechanisms of lipid synthesis and storage in hazelnuts.Several gene families related to salicylic acid metabolism and stress responses experienced rapid expansion in this hazelnut species,which may have increased its stress tolerance.The reference genome presented here constitutes a valuable resource for molecular breeding and genetic improvement of the important agronomic properties of hazelnut.
基金the National Natural Science Foundation of China(grants numbers 31772530,31970467 and 31872298)Strategic Priority Research Program of Chinese Academy of Sciences(Grant No.XDPB16).
文摘Endoparasitoid wasps introduce venom into their host insects during the egglaying stage.Venom proteins play various roles in the host physiology,development,immunity,and behavior manipulation and regulation.In this study,we identified a venom protein,MmRhol,a small guanine nucleotide-binding protein derived from ovary in the endoparasitoid wasp Microplitis mediator and found that knockdown of its expression by RNA interference caused down-regulation of vitellogenin and juvenile hormone,egg production,and cocoons formation in the female wasps.We demonstrated that MmRho1 entered the cotton bollworm's(host)hemocytes and suppressed cellular immune responses after parasitism using immunofluorescence staining.Furthermore,wasp MmRhol interacted with the cotton bollworm's actin cytoskeleton rearrangement regulator diaphanous by yeast 2-hybrid and glutathione s-transferase pull-down.In conclusion,this study indicates that MmRho1 plays dual roles in wasp development and the suppression of the host insect cellular immune responses.
基金the National Natural Science Foundation of China(32072623,31700611)the Natural Science Foundation of Anhui Province-Outstanding Youth Project(2008085J18).
文摘As the major bitter-and astringent-tasting components in tea leaves,catechins play an essential role in determining flavor characteristics and health benefits of tea products.This study analyzed the circadian rhythm effects on the accumulation pattern of catechins in tender leaves of three tea cultivars,'Huangkui'(HK),'Longjing43'(LJ43)and'Fuding Dabaicha'(FD).There were two major factors involved in the catechin regulation in tea plants:genetic background of the cultivar-specific regulation,and temporal effects of the circadian rhythm.Etiolated leaves of'HK'contained lower levels of catechins than'LJ43'and'FD'.The composition of EGCG in total catechins was significantly higher in'LJ43'leaves(65%)when compared with that in'HK'and'FD'(45%).The accumulation of individual catechins increased during the daytime but decreased overnight,especially over the time period of 22:00−02:00.The correlation analysis between catechins and environmental factors indicated catechin accumulation in tea leaves potentially resulted from a combined regulation of light and temperature.These findings provide new insights into our understanding of circadian rhythm regulation on flavor-important secondary metabolites in tea leaves.
基金This work was supported by the Key Research and Development Program of Guangdong Province(No.2018B030339001)Beijing Science and Technology Program(No.Z201100004420015)+1 种基金the National Natural Science Foundation of China(Nos.61601028 and 61431007)the National Key Research and Development Program of China(No.2017YFB1002505).
文摘For this research,electroencephalography(EEG)was analyzed to investigate the perception ability of the brain for moving objects at different speeds.In this experiment,total six kinds of videos regarding license plates were created,moving at distinct speed of 0.26 m/s,0.36 m/s,0.46 m/s,0.56 m/s,0.66 m/s,and 0.76 m/s,respectively.In the semantic priming paradigm,the N400 effect was analyzed for each speed.The ERP results demonstrated that the N400 amplitude gradually reduced with increasing speed.At the three lower speeds,N400 was evoked evidently and mainly distributed in the centro-posterior region.At the three higher speeds,no significant N400 effect was found.The results concluded that the perception ability of the brain declined with the acceleration of the object’s moving speed and that the brain recognized the detailed information of the moving object when its speed was lower than 0.46 m/s.
基金This work was equally supported by the National Natural Science Foundation of China(31590821)the Strategic Priority Research Program of the Chinese Academy of Sciences(grant no.XDB31010300)+3 种基金the National Key Research and Development Program of China(2017YFC0505203)further by the Second Tibetan Plateau Scientific Expedition and Research(STEP)program(2019QZKK0502)National Natural Science Foundation of China(91731301,41471042,and 31561123001)Fundamental Research Funds for the Central Universities(2020SCUNL207,SCU2018D006,and SCU2019D013).
文摘It is increasingly realized that homoploid hybrid speciation(HHS),which involves no change in chromosome number,is an important mechanism of speciation.HHS will likely increase in frequency as ecological and geographical barriers between species are continuing to be disrupted by human activities.HHS requires the establishment of reproductive isolation between a hybrid and its parents,but the underlying genes and genetic mechanisms remain largely unknown.In this study,we reveal by integrated approaches that reproductive isolation originates in one homoploid hybrid plant species through the inheritance of altemate alleles at genes that determine parental premating isolation.The parent species of this hybrid species are reproductively isolated by differences in flowering time and survivorship on soils containing high concentrations of iron.We found that the hybrid species inherits alleles of parental isolating major genes related to flowering time from one parent and alleles of major genes related to iron tolerance from the other parent.In this way,it became reproductively isolated from one parent by the difference in flowering time and from the other by habitat adaptation(iron tolerance).These findings and further modeling results suggest that HHS may occur relatively easily via the inheritance of alternate parental premating isolating genes and barriers.
基金Acknowledgments This work was supported by National Basic Research Program of China (No. 2014CB138405), Strategic Priority Research Program of CAS (No. XDB 11030600), National Natural Science Foundation of China (No. 31472008, 31401804, 31272497), Open Research Fund Program of State Key Laboratory of Integrated Pest Management (Chinese IPM1407, 1304).
文摘Peptidoglycan recognition proteins (PGRPs) are a family of innate immune receptors that specifically recognize peptidoglycans (PGNs) on the surface of a number of pathogens. Here, we have identified and characterized six PGRPs from endoparasitoid wasp, Microplitis mediator (MmePGRPs). To understand the roles of PGRPs in parasitoid wasps, we analyzed their evolutionary relationship and orthology, expression profiles during different developmental stages, and transcriptional expression following infection with Gram-positive and -negative bacteria and a fungus. MmePGRP-S1 was significantly induced in response to pathogenic infection. This prompted us to evaluate the effects of RNA interference mediated gene specific knockdown ofMmePGRP-S1. The knockdown of MmePGRP-S1 (iMmePGRP-S1) dramatically affected wasps' survival following challenge by Micrococcus luteus, indicating the involvement of this particular PGRP in immune responses against Gram-positive bacteria. This action is likely to be mediated by the Toll pathway, but the mechanism remains to be determined. MmePGRP-S 1 does not play a significant role in anti-fungal immunity as indicated by the survival rate of iMmePGRP-S wasps. This study provides a comprehensive characterization of PGRPs in the economically important hymenopteran species M. mediator.
基金Acknowledgments This study was financially supported by the National Natural Science Foundation of China (No. 31272497).
文摘Bacterial infection causes enhanced reactive oxygen species (ROS) levels in insects. Oxidation resistance 1 (OXR1) plays an antioxidant role in eukaryotic organisms, including insects. In this report, we demonstrated that Pseudomonas aeruginosa and Staphylococcus aureus infection and hydrogen peroxide (H2O2) injection induced the expression of specific transcriptional isoforms of OXR1 in larval silkworms. We further showed that a Jun kinase (JNK) pathway inhibitor, SP600125, down-regulated expression of OXRI during infection, leading to elevated H2O2 levels in the hemolymph, resulting in lower viability of the injected bacteria inside the silkworm larvae. Our study suggests that OXR1 participates in protecting larval silkworms from oxidative stress and bacterial infection through the JNK pathway.
文摘To analyze the effect of energy conservation policies on energy consumption of residential buildings, the characteristics of energy consumption and indoor thermal comfort were investigated in detail in Tianjin, China, based on official statistical yearbook and field survey data. A comprehensive survey of 305 households indicates that the mean electricity consumption per household is 3215kWh/a, in which annual cooling electricity consumption is 344kWh/a, and the mean natural gas consumption for cooking is 103.2m3/a. Analysis of 3966 households data shows that space heating average intensity of residential buildings designed before 1996 is 133.7kWh/(m^2.a), that of buildings designed between 1996 and 2004 is 117.2 kWh/(m^2.a), and that of buildings designed after 2004 is 105.0kWh/(m^2.a). Apparently, enhancing the performance of envelops is effective in reducing space heating intensity. Furthermore, the results of questionnaires show that 18% of the residents feel slightly warm and hot respectively, while 3% feel slightly cold in winter. Therefore, the electricity consumption in summer will rise for meeting indoor thermal comfort.