4种检测方法对骨结核的诊断价值比较

目的探讨涂片抗酸染色、TB-DNA、Xpert MTB/RIF、结核菌培养等4种检测方法对骨结核诊断的价值对比。方法对骨外科1 a内住院骨质破坏患者的病灶周围脓液,用4种检测方法进行检测,并对结果进行统计分析,指标包括:灵敏度、特异度、阳性预测值、阴性预测值。结果研究对象中以临床诊断为金标准,脓液涂片查抗酸杆菌灵敏度为31.75%,特异度为100.00%,阳性预测值100.00%,阴性预测值53.74%,约登指数为0.32;TB-DNA灵敏度为88.89%,特异度为98.00%,阳性预测值98.25%,阴性预测值87.50%,约登指数为0.87;Xpert MTB/RIF灵敏度为95.23%,特异度为68.00%,阳性预测值78.95%,阴性预测值91.90%,约登指数为0.63;脓液结核菌培养灵敏度为41.27%,特异度为100.00%,阳性预测值100.00%,阴性预测值57.47%,约登指数为0.41,(χ^(2)=77.354,P<0.005)4种检测方法差异具有统计学意义。结论4种检测方法中,TB-DNA灵敏度和特异度较好,Xpert MTB/RIF灵敏度较好,TB-DNA和Xpert MTB/RIF的真实性较好,同时阳性预测值和阴性预测值较高,对骨结核的诊断具有较好的价值。

Compact SPAD pixels with fast and accurate photon counting in the analog domain

A compact pixel for single-photon detection in the analog domain is presented. The pixel integrates a single-photon avalanche diode(SPAD), a passive quenching & active recharging circuit(PQARC), and an analog counter for fast and accurate sensing and counting of photons. Fabricated in a standard 0.18 μm CMOS technology, the simulated and experimental results reveal that the dead time of the PQARC is about 8 ns and the maximum photon-counting rate can reach 125 Mcps(counting per second). The analog counter can achieve an 8-bit counting range with a voltage step of 6.9 mV. The differential nonlinearity(DNL) and integral nonlinearity(INL) of the analog counter are within the ± 0.6 and ± 1.2 LSB, respectively, indicating high linearity of photon counting. Due to its simple circuit structure and compact layout configuration, the total area occupation of the presented pixel is about 1500 μm^(2), leading to a high fill factor of 9.2%. The presented in-pixel front-end circuit is very suitable for the high-density array integration of SPAD sensors.

Application of Ultimate Oncoplastic Surgery in Breast-Conserving Surgery

Objective To investigate the application of oncoplastic surgery in breast-conserving surgery.Methods We retrospectively analyzed the clinical data of 103 breast cancer patients who underwent breast-conserving surgery in the First Affiliated Hospital of Henan University.All the patients were female whose tumor volume-to-breast volume ratio was greater than 20%.Fifty-two patients were treated with oncoplastic breast-conversing surgery(observation group),and 51 patients were treated with traditional breast-conserving surgery(control group).The volume of resected tissue,subjective satisfaction with breast shape,objective score of breast shape,and follow-up were compared between the two groups.Results In the observation group,the weight of resected breast tissue was 64.2–172.1 g,with a median of 98.7 g.In the control group,the weight of resected breast tissue was 67.5–175.7 g,with a median of 102.3 g.After 12 months of follow-up,the subjective satisfaction rate and objective score of breast shape in the observation group were significantly better than those in the traditional breast-conserving surgery group(P<0.05).There was no recurrence,metastasis,or death in the two groups.There was no significant difference in postoperative complications between the two groups(P>0.05).Conclusion Oncoplastic breast-conserving surgery leads to better cosmetic results and a more satisfactory clinical results.

紫檀芪抗肿瘤作用机制研究进展

紫檀芪(3,5-二甲氧基-4’-羟基二苯乙烯)是一种主要存在于蓝莓、葡萄和花榈木中的多酚类化合物。已有的研究发现紫檀芪具有抗肺癌、乳腺癌、胃癌、结肠癌等多种肿瘤的抗癌作用。其作用机制涉及调控影响多种肿瘤生物学特性。此外,紫檀芪具有比白藜芦醇更高的生物利用度和生物活性,其抗肿瘤作用和机制值得深入探讨和研究。

全科医生临床能力培训效果评价及影响因素分析

目的:探讨临床能力培训对全科医生综合水平提高的效果并分析其可能的影响因素。方法:从2020年2月份起到次年9月份期间某社区的全科医生中随机抽取84名,由当地三级综合医院职称在副主任及以上的全科医师对其就临床能力进行相关培训。观察比较全科医生培训前后职业素质、病史采集、病例分析、体格检查、基本操作和辅助检查等临床能力的改变及其所服务的患者的满意度。结果:全科医生在培训后在职业素质、病史采集、病例分析、体格检查、基本操作和辅助检查上的得分均高于培训前(P<0.05),其主要影响因素有工作年限,培训前临床能力得分及学历有关,其所服务患者的满意度在培训后有较明显的提高(P<0.05)。结论:对全科医生进行继续教育,临床能力的再次培训,能有效提高其临床实践能力,为患者提供更好的服务,建议推广使用。

Arabidopsis AtBECLIN 1/AtAtg6/AtVps30 is essential for pollen germination and plant development

兼容丑恶纸巾的表面上的花粉萌芽是为植物授精的必要的步。这里，我们报导 Arabidopsis 变异的 bcl1 是由于花粉萌芽的失败不育的男性。我们证明 bcl1 异种等位基因不能被男配偶体播送，没有同型结合的 bcl1 异种被获得。花粉发育阶段的分析显示 bcl1 变化影响花粉萌芽然而并非花粉成熟。分子的分析证明花粉萌芽的失败被 AtBECLIN 1 的混乱引起。AtBECLIN 1 在成熟花粉主要被表示并且与重要相同编码蛋白质到在酵母为 autophagyand vacuolar 蛋白质排序的过程(VPS ) 要求的 Beclin1/Atg6/Vps30。我们也证明 AtBECLIN 1 为正常被要求植物开发，和那与 autophagy， VPS 和 glycosylphosphatidylinositolanchor 系统有关的基因，被 AtBECLIN 1 的缺乏影响。

Grain refinement of Mg-Al alloys inoculated by MgAl_(2)O_(4)powder

As a potent nucleating substrate forα-Mg grain,MgAl_(2)O_(4) powder was used to inoculate the Mg-Al melt in this study.The effects of MgAl_(2)O_(4)amount,holding time and Al content on the grain size and grain refining ratio of the inoculated Mg-Al alloys are systematically investigated.The results show that the minimum grain size of Mg-3Al alloy is achieved by adding 2wt.%MgAl_(2)O_(4)powder and this alloy exhibits higher grain refining ratio than Mg-5Al and Mg-8Al alloys.The crystallographic misfit calculation indicates the wellmatching and possible orientation relationships(ORs)betweenα-Mg and MgAl_(2)O_(4).Among these predicted ORs,[10–10]α−Mg//[110]MgAl2O4 in(0002)α−Mg//(1–13)MgAl2O4 possesses the smallest misfit,i.e.,2.34%(fr).Both results of the experiment and crystallographic calculation demonstrate that the grain refinement of Mg-Al alloys is attributed to the MgAl_(2)O_(4)particles acting as the heterogeneous nucleation substrates forα-Mg grains.

Accumulation of residual soil microbial carbon in Chinese fir plantation soils after nitrogen and phosphorus additions

Nitrogen （N） and phosphorus （P） additions can affect soil microbial carbon （C） accumulation. However, the mechanisms that drive the changes in residual microbial C that occur after N and P additions have not been well-defined for Chinese fir plantations in subtropical China. We set up six different treatments, viz. a control （CK）, two N treatments （NI： 50kgha-1 a-1; N2： 100 kg ha-1 a-1）, one P treatment （P： 50 kg ha-1 a-1）, and two combined N and P treatments （NIP： 50kgha-1a-1 of N ＋50kgha-1a-1 of P; N2P：100 kg ha-1 a-1 of N ＋ 50 kg ha-1 a-1 of P）. We then investigated the influences of N and P additions on residual microbial C. The results showed that soil pH and microbial biomass decreased after N additions, while microbial biomass increased after P additions. Soil organic carbon （SOC） and residual microbial C contents increased in the N and P treatments but not in the control. Residual microbial C accumulation varied according to treatment and declined in the order： N2P 〉 N1P 〉 N2 〉 N1 〉 P 〉 CK. Residual microbial C contents were positively correlated with available N, P, and SOC contents, but were negatively correlated with soil pH. The ratio of residual fungal C to residual bacterial C increased under P additions, but declined under combined N1P additions. The ratio of residual microbial C to SOC increased from 11 to 14% under the N1P and N2P treatments, respectively. Our results suggest that the concentrations of residual microbial C and the stability of SOC would increase under combined applications of N and P fertilizers in subtropical Chinese fir plantation soils.

Assay for the developmental toxicity of safflower (Carthamus tinctorius L.) to zebrafish embryos/larvae

Objective:To explore the developmental toxicity and the potential toxicological mechanism of safflower (Carthamus tinctorius L.) on zebrafish embryos/larvae.Methods:Mortality,malformations and increased apoptosis induced by safflower were assessed in zebraflsh embryos from 6 to 96 hours post-fertilization.Enzymes and genes in the anti-oxidative and apoptotic pathways were also assayed.Results:The lethal concentration 50 of safflower to zebrafish embryos was 345.6 mg/L.Hatching inhibition,abnormal spontaneous movement,depressed heart rate,pericardial edema,yolk sac edema,abnormal head-trunk angle,inhibition of melanin release,enlarged yolk,and short body length were observed in safflower-treated zebrafish.Additional apoptotic cells mainly appeared around the heart.Safflower exposure changed the activities of defense enzymes (SOD T,CAT↑,MDA↑,GPX without trend),increased MDA content,decreased caspase-3 activity,and altered mRNA levels of related genes (ogg1 ↓,p53↓,Cu/Zn-sod↑,Mn-sod↓,cat↓,gpx↑).Conclusion:Safflower exhibits developmental toxicity for zebrafish embryos/larvae.The developing heart was speculated as the target organ of toxicity.Oxidative stress and increased apoptosis have roles in the developmental toxicity of safflower.This article provides a novel method to research the teratogenicity and possible mechanisms of toxicity of traditional Chinese medicines that are prohibited or contraindicated in pregnant women.

An Explicit-Implicit Mixed Staggered Asynchronous Step Integration Algorithm in Structural Dynamics

Many engineering applications need to analyse the system dynamics on the macro and micro level,which results in a larger computational effort.An explicit-implicit asynchronous step algorithm is introduced to solve the structural dynamics in multi-scale both the space domain and time domain.The discrete FEA model is partitioned into explicit and implicit parts using the nodal partition method.Multiple boundary node method is adopted to handle the interface coupled problem.In coupled region,the implicit Newmark coupled with an explicit predictor corrector Newmark whose predictive wave propagates into the implicit mesh.During the explicit subcycling process,the variables of boundary nodes are solved directly by dynamics equilibrium equation.The dissipation energy is dynamically determined in accordance with the energy balance checking.A cantilever beam and a building two numerical examples are proposed to verify that the method can greatly reduce the computing time while maintaining a high accuracy.

A deformability-based biochip for precise label-free stratification of metastatic subtypes using deep learning

Cellular deformability is a promising biomarker for evaluating the physiological state of cells in medical applications.Microfluidics has emerged as a powerful technique for measuring cellular deformability.However,existing microfluidic-based assays for measuring cellular deformability rely heavily on image analysis,which can limit their scalability for high-throughput applications.Here,we develop a parallel constriction-based microfluidic flow cytometry device and an integrated computational framework(ATMQcD).The ATMQcD framework includes automatic training set generation,multiple object tracking,segmentation,and cellular deformability quantification.The system was validated using cancer cell lines of varying metastatic potential,achieving a classification accuracy of 92.4%for invasiveness assessment and stratifying cancer cells before and after hypoxia treatment.The ATMQcD system also demonstrated excellent performance in distinguishing cancer cells from leukocytes(accuracy=89.5%).We developed a mechanical model based on power-law rheology to quantify stiffness,which was fitted with measured data directly.The model evaluated metastatic potentials for multiple cancer types and mixed cell populations,even under realworld clinical conditions.Our study presents a highly robust and transferable computational framework for multiobject tracking and deformation measurement tasks in microfluidics.We believe that this platform has the potential to pave the way for high-throughput analysis in clinical applications,providing a powerful tool for evaluating cellular deformability and assessing the physiological state of cells.

Implementation of Dijkstra’s Token Circulation on Sensor Network

Sensor networks can consist of large number of sensors. Often, sensors networks use low cost units and thus a subject to malfunctions that can bring the system to inconsistent states. After deployment, the system can be situated in places that are hard to reach and therefore manual reboot operations are undesirable and even unfeasible. Therefore, it is imperative to consider the eventual recovery of arbitrary fault when designing sensor networks. Dijkstra’s algorithm is an important foundation of self-managing computer system and fault-tolerance computing system in distributed systems, since it allows a distributed system to recover from arbitrary starting state within a finite time. The arbitrary starting state ca model arbitrary failure (as long as the code segment stays correct).Another key advantage of Dijkstra’s asynchronous algorithm is that no global clock is needed. This project tests an implementation of Dijkstra’s algorithm using snapshotting techniques that we developed in an earlier work. These sensors can initiate from any state but they come into a consistent one after several cycles of running. We demonstrate the usefulness of our testing technique.

Exquisite structure of the lateral line system in eyeless cavefish Sinocyclocheilus tianlinensis contrast to eyed Sinocyclocheilus macrophthalmus(Cypriniformes:Cyprinidae)

In this study,the lateral line systems in Chinese cavefish eyeless Sinocyclocheilus tianlinensis and eyed Sinocyclocheilus macrophthalmus were investigated to reveal their morphological changes to survive in harsh environments.Compared with the eyed cavefish S.macrophthalmus(atypical),the lateral line system in the eyeless cave-fish S.tianlinensis(typical)has certain features to adapt to the dark cave environments:the superficial lateral line system in the eyeless species possesses a higher number of superficial neuromasts and more hair cells within an individual neuromast,and the trunk lateral line canal system in S.tianlinensis exhibits larger canal pores,higher canal diameter and more pronounced constrictions.Fluid–structure interaction analysis suggested that the trunk lateral line canal system in the eyeless S.tianlinensis should be more sensitive than that in the eyed S.macrophthalmus.These morphological features of the lateral line system in the eyeless S.tianlinensis probably enhance the functioning of the lateral line system and compensate for the lack of eyes.The revelation of the form–function relationship in the cavefish lateral line system provides inspiration for the design of sensitive artificial flow sensors.

Influence of circulation types on temporal and spatial variations of ozone in Beijing

This study analyzes the impact of circulation types(CTs)on ozone(O_(3))pollution in Beijing.The easterly high-pressure(SWW)circulation occurred most frequently(30%;276 day),followed by northwesterly high-pressure(AN)circulation(24.3%;224 day).The SWW type had the highest O_(3) anomaly of+17.28μg/m^(3),which was caused by excellent photochemical reactions,poor diffusion ability and regional transport.Due to the higher humidity and precipitation in the low-pressure type(C),the O_(3) increase(+8.02μg/m^(3))was less than that in the SWW type.Good diffusion/wet deposition and weak formation ability contributed to O_(3) decrease in AN(-12.54μg/m^(3))and northerly high-pressure(ESN)CTs(-12.26μg/m^(3)).The intra-area transport of O_(3) was significant in polluted circulations(SWW-and C-CTs).In addition,higher temperature,radiation and less rainfall also contributed to higher O_(3) in northern Beijing under the SWW type.For the clean CTs(AN and ESN CTs),precursor amount and intra-area transport played a dominant role in O_(3) distribution.Under the northeasterly low-pressure CT,better formation conditions and higher precursor amount combined with the intra-area southerly transport to cause higher O_(3) values in the south than in the north.The higher O_(3) in the northwestern area under the northeasterly high-pressure type was influenced by weaker titration loss and high O_(3) concentration in previous day.Annual variation in the CTs contributed up to 86.1%of the annual variation in O_(3).About 78%-83%of the diurnal variation in O_(3) resulted from local meteorological factors.

Emerging role of ubiquitination/deubiquitination modification of PD-1/PD-L1 in cancer immunotherapy

As members of the immune checkpoint family, PD-1 and its ligand PD-L1 play critical roles in maintaining the balance between autoimmunity and tolerance. The interaction of PD-1/PD-L1 is also involved in tumor evasion inside the tumor microenvironment, caused by reduced T cell activation, proliferation, cytotoxic secretion, and survival. Previous research has shown that the expression level of PD-1/PD-L1 may be regulated by ubiquitin-mediated proteasome degradation, which is an important mode of post-translational modification (PTM). PD-1/PD-L1 ubiquitin modification research in tumor immunotherapy is the subject of the present review, which aims to assess the most recent developments in this area. We offer a short explanation of PD-1/PD-L1 as well as some basic background information on the UPS system and discuss many routes that target E3s and DUBs, respectively, in the regulation of PD-1/PD-L1 in tumor immunotherapy. In addition, we offer numerous innovative prospective research areas for the future, as well as novel immunotherapy concepts and ideas. Taken together, the information compiled herein should serve as a comprehensive repository of information about tumor immunotherapy that is currently available, and it should be useful in the design of future studies, as well as the development of potential targets and strategies for future tumor immunotherapy.

Variation in Sunshine Duration and Related Aerosol Influences at Shangdianzi GAW Station, China: 1958–2021

Sunshine duration(SD) is adopted widely to study global dimming/brightening. However, long-term simultaneous measurements of SD and closely related impact factors require further analysis to elucidate how and why SD has varied during the past decades. In this study, a long-term(1958–2021) SD data series obtained from the Shangdianzi Global Atmosphere Watch(GAW) station in China was analyzed to detect linear trends, climatic jumps, and climatic periods in SD using linear fitting, the Mann–Kendall trend test, and the continuous wavelet transform method. Annual SD exhibited steady dimming(-67.3 h decade-1) before 2010, followed by a period of brightening(189.9 h decade-1)during 2011–2020. An abrupt jump in annual SD occurred in 1995, and the annual SD anomaly exhibited significant oscillation with ~3-yr periodicity during 1960–1978. Partial least squares analysis revealed that annual SD anomaly was associated with variations in relative humidity, gale days, cloud cover, and black carbon(BC). Further analysis of the clear-sky daily sunshine percentage(DSP) and simultaneous measurements of aerosol properties, including aerosol optical depth, aerosol extinction coefficient, single scattering albedo(SSA), BC, and total suspended particulates, suggested that variation in DSP was affected primarily by aerosol scattering and absorption. Furthermore, the hourly clear-sky SD at high aerosol loading was approximately 60% and 56% of that at middle and low aerosol loadings, respectively. The pattern of diurnal variation in clear-sky hourly SD, as well as the actual values, can be affected by the fine particulate concentration, aerosol extinction coefficient, and SSA.

Outlier Detection Model Based on Autoencoder and Data Augmentation for High-Dimensional Sparse Data

This paper aims to address the problems of data imbalance,parame-ter adjustment complexity,and low accuracy in high-dimensional data anomaly detection.To address these issues,an autoencoder and data augmentation-based anomaly detection model for high-dimensional sparse data is proposed(SEAOD).First,the model solves the problem of imbalanced data by using the weighted SMOTE algorithm and ENN algorithm tofill in the minority class samples and generate a new dataset.Then,an attention mechanism is employed to calculate the feature similarity and determine the structure of the neural network so that the model can learn the data features.Finally,the data are dimensionally reduced based on the autoencoder,and the sparse high-dimensional data are mapped to a low-dimensional space for anomaly detection,overcoming the impact of the curse of dimensionality on detection algorithms.The experimental results show that on 15 public datasets,this model outperforms other comparison algorithms.Furthermore,it was validated on industrial air quality datasets and achieved the expected results with practicality.

我国大气本底观测站创新发展的思考和建议

大气本底监测是指在远离人类活动影响的地方对经过充分混合的、不受局地污染影响的大气成分进行长期定点的观测.由于大气本底监测能在全球或区域尺度上反映出人类活动所造成的大气成分的长期变化,因此对社会经济建设和科学研究等都具有非常重要的作用[1~3].自20世纪50年代开始,国外的一些观测台站进行了二氧化碳、臭氧及大气重金属等主要大气成分的本底观测[4~8].

智能时代教师设计思维培养:逻辑向度与困境纾解

在智能时代,技术赋能教育教学的方式与内容不断更新,智能教育创新发展在一定程度上呼唤教师设计思维的有效培育。基于教师设计思维的内涵诠释,提出智能时代教师设计思维培养的逻辑向度,发现智能时代教师设计思维培养面临一定困境:一是机器学习机械化挖掘认知经验,忽视教师设计思维的情境化培养;二是过度依赖智能运算技术,易生成僵硬的线性思维;三是智能教育资源同质化推送,削弱教师创造能动性与热情;四是智能空间感知受困于浅层情感捕捉,忽视教师的情感互动和体验。据此,提出智能时代背景下教师设计思维的培养建议:一是在反思性情境中培养教师的创新知能,构建与直接经验关联的设计思维模式;二是增强教师对智能教育的理解与批判力,培育兼具“软”“硬”技能的设计者;三是树立理性批判与价值融合的资源观,构建“智能+资源设计”服务体系;四是加强情感互动与人文关怀,设计共享创意的智能教育空间。