Isolation, Identification and Antimicrobial Test in vitro of Aeromonas from Hemibarbus maculatus Bleeker

[Objective] The aim was to quickly find high free drugs to prevent Hemibarbus maculates Bleeker from dying. [Method] The conventional biochemical methods and molecular biological identification method was used to do the isolation and identification of bacteria from H. maculates, and agar diffusion method was used for the susceptibility test and in vitro becteriostasis experiment. The toxins were detected using the plate method, and the intraperitoneal injection method was used to do animal experiments. [Result] Twelve strains of isolated bacteria were obtained, which could uniformly decompose glucose, maltose, but were unable to break down lactose, and they could reduce the nitrate, and were negative in hydrogen sulfide and indole. A clear band was found in 685 bp of the isolated bacteria. And the isolated bacterial all generated hemolysins. Some strains produced protease. The isolated bacterial were resistant to penicillin G, amoxicillin, but sensitive to gentamicin, ciprofloxacin, and could be inhibited by the Bacillus amyloliquefaciens. All experimental animals died within 12 hours. [Conclusion] The 12 isolates were Aeromonas veronii, Aeromonas caviae and Aeromonas hydrophila, respectively, and the deaths of H. maculates were caused by the mixed infection of Aeromonas veronii, Aeromonas caviae and Aeromonas hydrophila. The first choice drug for the treatment was ciprefloxacin, and Bacillus amyloliquefeciens could be used as the drug for ecological prevention and control.

Hybrid graphene metasurfaces for highspeed mid-infrared light modulation and single-pixel imaging

During the past decades,major advances have been made in both the generation and detection of infrared light;however,its efficient wavefront manipulation and information processing still encounter great challenges.Efficient and fast optoelectronic modulators and spatial light modulators are required for mid-infrared imaging,sensing,security screening,communication and navigation,to name a few.However,their development remains elusive,and prevailing methods reported so far have suffered from drawbacks that significantly limit their practical applications.In this study,by leveraging graphene and metasurfaces,we demonstrate a high-performance free-space mid-infrared modulator operating at gigahertz speeds,low gate voltage and room temperature.We further pixelate the hybrid graphene metasurface to form a prototype spatial light modulator for high frame rate single-pixel imaging,suggesting orders of magnitude improvement over conventional liquid crystal or micromirror-based spatial light modulators.This work opens up the possibility of exploring wavefront engineering for infrared technologies for which fast temporal and spatial modulations are indispensable.