Avian influenza (AI) virology surveillance is the most important method to monitor AI virus (AIV) in poultry so as to effectively prevent and control AI outbreaks. Monoclonal antibodies (MAb)-based assays are highly s...Avian influenza (AI) virology surveillance is the most important method to monitor AI virus (AIV) in poultry so as to effectively prevent and control AI outbreaks. Monoclonal antibodies (MAb)-based assays are highly sensitive and specific for AIV detection, and much practical and economic for test-in-field or onsite. Many such assays have been developed and are still in developing since the H5N1 highly pathogenic AI (HPAI) outbreaks occurred in South East Asia in 2003. A MAb-based dot-enzyme-linked immunosorbent assay (ELISA) has been developed in our lab during late 1990s and early 2000s. Meanwhile, AIV H7 and H5 subtype specific-MAbs have been successfully developed in our laboratory to enhance the Dot-ELISA and other MAb-based assays for AIV detection. Production and purification of the H7 and H5 MAbs were made to provide essential reagents for Dot-ELISA and other immunoassays, and the current development of a novel Biosensor technique for rapid detection of AIV from clinical and field specimens.展开更多
基金We thank Dr Bin Zhang (University of Michigan, USA) for providing the MCFD2 antibodies. This work was supported in part by the Life Science Special Fund of the Chinese Academy of Sciences for Human Genome Research (KJ95T-06 and KSCX1-Y02 to BML, NHJ and MLJ), the National Natural Science Foundation of China (30225023 and 30430240 to BML and 90208011, 30300174, 30470856, 30421005 and 30623003 to NHJ), the National Key Basic Research and Development Program of China (2006CB500807 to BML and 2002CB713802, 2005CB522704 and 2006CB943902 to NHJ), the National High-Tech Research and Development Program of China (2006AA02ZI99 to BML and 2006AA02Z186 to NHJ), the Shanghai Key Project of Basic Science Research (04DZ14005 to BML and 04DZ14005, 04DZ05608, 06DJI4001 and 06DZ22032 to NHJ), the Council of the Shanghai Municipal for Science and Technology (05814578 to NHJ), and the US National Institutes of Health (DA013471 and DA020555 to LY).
文摘Avian influenza (AI) virology surveillance is the most important method to monitor AI virus (AIV) in poultry so as to effectively prevent and control AI outbreaks. Monoclonal antibodies (MAb)-based assays are highly sensitive and specific for AIV detection, and much practical and economic for test-in-field or onsite. Many such assays have been developed and are still in developing since the H5N1 highly pathogenic AI (HPAI) outbreaks occurred in South East Asia in 2003. A MAb-based dot-enzyme-linked immunosorbent assay (ELISA) has been developed in our lab during late 1990s and early 2000s. Meanwhile, AIV H7 and H5 subtype specific-MAbs have been successfully developed in our laboratory to enhance the Dot-ELISA and other MAb-based assays for AIV detection. Production and purification of the H7 and H5 MAbs were made to provide essential reagents for Dot-ELISA and other immunoassays, and the current development of a novel Biosensor technique for rapid detection of AIV from clinical and field specimens.