Sepsis is responsible for approximately 5.3 million deaths globally each year.Here,we constructed hierarchical chitin microspheres loaded with MOF-919(Ch/metal–organic frameworks[MOFs])for the rapid and efficient rem...Sepsis is responsible for approximately 5.3 million deaths globally each year.Here,we constructed hierarchical chitin microspheres loaded with MOF-919(Ch/metal–organic frameworks[MOFs])for the rapid and efficient removal of lipopolysaccharide(LPS)in complex blood environments.Furthermore,abun-dant active sites on MOF-919(Sc)also enable a record-high adsorption capacity of 9.56 mg/g in biomass-based adsorbents due to the coordination interactions between endotoxin and MOF-919(Sc).The LPS level of sepsis rabbits was less than 2 EU/mL(clearance rate>95%)after 90-min hemoperfusion,showing no adverse effect on the rabbit organs.Additionally,compared to the commonly used LPS scrubber Toraymyxin(polymethyl methacrylate),the chitin adsorbent is significantly more cost-effective and environmentally friendly.The prepara-tion strategy for hierarchical porous microspheres offers notable advantages in designability,recyclability,and renewability,providing a new approach to sepsis treatment and promising prospects for the biomedical application of sustainable biomass materials.展开更多
Repeated waves of influenza virus H7N9 epidemics after 2013 have caused severe influenza in humans,with mortality reaching approximately 40%–50%.To prevent possible pandemics,the development of highly effective vacci...Repeated waves of influenza virus H7N9 epidemics after 2013 have caused severe influenza in humans,with mortality reaching approximately 40%–50%.To prevent possible pandemics,the development of highly effective vaccines against influenza virus H7N9 is highly desired.In the present study,by taking advantage of the D-tetra-peptide adjuvant(G^(D)F^(D)F^(D)Y),we reported a simple method to prepare H7N9 vaccines.Naproxen(Npx),with good anti inflammatory and broad anti-viral effects,was employed as an N-terminal capping group to construct a hydrogel precursor,Npx-G^(D)F^(D)F^(D)Y.The hydrogel adjuvant was prepared using a routine heating cooling protocol and the final vaccine was ready after mixing with the split A/Zhejiang/DTID-ZJU01/2013(H7N9)antigen by vortexing.Compared with the traditional Al(OH)_(3) adjuvant vaccine and the split vaccine,our hydrogel adjuvant vaccine showed the best preventive effects against H7N9 infection.A mechanistic study illustrated that higher antibody responses and variations in cytokine expression might account for its increased protective effects.Our strategy demonstrated the advantages of a peptide hydrogel adjuvant in the application of vaccines against H7N9 and demonstrated its potential application in vaccines against emerging threats from other viruses.展开更多
基金National Natural Science Foundation of China,Grant/Award Number:22075215Key Research and Development Program of Hubei Province,Grant/Award Number:2021BCA122+1 种基金Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University,Grant/Award Number:ZNJC202238Fundamental Research Funds for the Central Universities,Grant/Award Number:691000003。
文摘Sepsis is responsible for approximately 5.3 million deaths globally each year.Here,we constructed hierarchical chitin microspheres loaded with MOF-919(Ch/metal–organic frameworks[MOFs])for the rapid and efficient removal of lipopolysaccharide(LPS)in complex blood environments.Furthermore,abun-dant active sites on MOF-919(Sc)also enable a record-high adsorption capacity of 9.56 mg/g in biomass-based adsorbents due to the coordination interactions between endotoxin and MOF-919(Sc).The LPS level of sepsis rabbits was less than 2 EU/mL(clearance rate>95%)after 90-min hemoperfusion,showing no adverse effect on the rabbit organs.Additionally,compared to the commonly used LPS scrubber Toraymyxin(polymethyl methacrylate),the chitin adsorbent is significantly more cost-effective and environmentally friendly.The prepara-tion strategy for hierarchical porous microspheres offers notable advantages in designability,recyclability,and renewability,providing a new approach to sepsis treatment and promising prospects for the biomedical application of sustainable biomass materials.
基金the support from the Medical and Health Science and Technology Program of Zhejiang Province,China(No.2020379356)the China Postdoctoral Science Foundation(No.2020T130102ZX)。
文摘Repeated waves of influenza virus H7N9 epidemics after 2013 have caused severe influenza in humans,with mortality reaching approximately 40%–50%.To prevent possible pandemics,the development of highly effective vaccines against influenza virus H7N9 is highly desired.In the present study,by taking advantage of the D-tetra-peptide adjuvant(G^(D)F^(D)F^(D)Y),we reported a simple method to prepare H7N9 vaccines.Naproxen(Npx),with good anti inflammatory and broad anti-viral effects,was employed as an N-terminal capping group to construct a hydrogel precursor,Npx-G^(D)F^(D)F^(D)Y.The hydrogel adjuvant was prepared using a routine heating cooling protocol and the final vaccine was ready after mixing with the split A/Zhejiang/DTID-ZJU01/2013(H7N9)antigen by vortexing.Compared with the traditional Al(OH)_(3) adjuvant vaccine and the split vaccine,our hydrogel adjuvant vaccine showed the best preventive effects against H7N9 infection.A mechanistic study illustrated that higher antibody responses and variations in cytokine expression might account for its increased protective effects.Our strategy demonstrated the advantages of a peptide hydrogel adjuvant in the application of vaccines against H7N9 and demonstrated its potential application in vaccines against emerging threats from other viruses.
