Botryosphaeria dieback is a serious disease problem for table and grape wine production worldwide.The disease however,has been less well-studied in China.In this study,we surveyed Botryosphaeria dieback in 72 vineyard...Botryosphaeria dieback is a serious disease problem for table and grape wine production worldwide.The disease however,has been less well-studied in China.In this study,we surveyed Botryosphaeria dieback in 72 vineyards of 20 grape-growing regions in China and found that Botryosphaeria dieback occurs in 18 out of 20 provinces.Morphological and multi-gene phylogenetic analyses confirmed that Botryosphaeria dothidea,Diplodia seriata,Lasiodiplodia theobromae and Neofusicoccum parvum are associated with different grapevine dieback symptoms.This study also revealed considerable differences in the geographical distribution of Botryosphaeriaceae species in China with Lasiodiplodia theobromae and Neofusicoccum parvum occurring only in subtropical monsoon climate regions,Diplodia seriata occurring only in temperate monsoon climate regions,and Botryosphaeria dothidea occurring in both temperate and subtropical monsoon climate regions.Analysis of 26 isolates showed that there is little genetic variation within species.Koch’s postulates were satisfied for all species,and pathogenicity testing showed that among the 25 major cultivars growing in China,none was resistant to the four taxa.The current paper represents the first detailed report on Botryosphaeria dieback of grapevines in China.展开更多
Grapevine anthracnose is an important disease,responsible for mild to severe yield losses in grape production,and is also an important post harvest disease.The disease was studied in vineyards in six provinces in Chin...Grapevine anthracnose is an important disease,responsible for mild to severe yield losses in grape production,and is also an important post harvest disease.The disease was studied in vineyards in six provinces in China,with 34 isolates obtained from diseased grapes.Multi-gene(ACT,ITS,GAPDH,TUB2 and CHS)analysis coupled with morphology showed that Colletotrichum aenigma,C.hebeiense sp.nov.and C.viniferum were associated with grapevine anthracnose in China.Colletotrichum aenigma is reported for the first time as associated with grapevine anthracnose.Colletotrichum hebeiense is a new species introduced here.Pathogenicity testing showed that all species can infect grapes,causing anthracnose however,virulence of species and isolates showed great variation.Phylogenetic analysis showed that C.viniferum is a cryptic species and its taxonomy needs to be resolved in the future.展开更多
文摘Botryosphaeria dieback is a serious disease problem for table and grape wine production worldwide.The disease however,has been less well-studied in China.In this study,we surveyed Botryosphaeria dieback in 72 vineyards of 20 grape-growing regions in China and found that Botryosphaeria dieback occurs in 18 out of 20 provinces.Morphological and multi-gene phylogenetic analyses confirmed that Botryosphaeria dothidea,Diplodia seriata,Lasiodiplodia theobromae and Neofusicoccum parvum are associated with different grapevine dieback symptoms.This study also revealed considerable differences in the geographical distribution of Botryosphaeriaceae species in China with Lasiodiplodia theobromae and Neofusicoccum parvum occurring only in subtropical monsoon climate regions,Diplodia seriata occurring only in temperate monsoon climate regions,and Botryosphaeria dothidea occurring in both temperate and subtropical monsoon climate regions.Analysis of 26 isolates showed that there is little genetic variation within species.Koch’s postulates were satisfied for all species,and pathogenicity testing showed that among the 25 major cultivars growing in China,none was resistant to the four taxa.The current paper represents the first detailed report on Botryosphaeria dieback of grapevines in China.
基金The study is funded by CARS-30.There are many people to thank for their assistance with this work.The authors would like to thank Miss.Qin Tian for the help given to perform the pathogenicity tests and Miss.K.W.T.Chethana for the help given in phylogenetic analysis.Authors would like to thank the grape cultivators who provided the diseased fruits.K.D.Hyde thanks the National Research Council of Thailand,Colletotrichum grant number 54201020003 and The Chinese Academy of Sciences,project number 2013T2S0030,for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany.This work was also supported by a grant from the National Plan of Science and Technology,King Abdulaziz City of Science and Technology,Riyadh,Saudi Arabia(10-Bio-965-02).M.M.R.S.Jayawardena would like to thank H.A.Ariyawansa,D.Udayanga for the support given.
文摘Grapevine anthracnose is an important disease,responsible for mild to severe yield losses in grape production,and is also an important post harvest disease.The disease was studied in vineyards in six provinces in China,with 34 isolates obtained from diseased grapes.Multi-gene(ACT,ITS,GAPDH,TUB2 and CHS)analysis coupled with morphology showed that Colletotrichum aenigma,C.hebeiense sp.nov.and C.viniferum were associated with grapevine anthracnose in China.Colletotrichum aenigma is reported for the first time as associated with grapevine anthracnose.Colletotrichum hebeiense is a new species introduced here.Pathogenicity testing showed that all species can infect grapes,causing anthracnose however,virulence of species and isolates showed great variation.Phylogenetic analysis showed that C.viniferum is a cryptic species and its taxonomy needs to be resolved in the future.
